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The map label for this gene is grpE [H]

Identifier: 222525489

GI number: 222525489

Start: 2829341

End: 2829940

Strand: Reverse

Name: grpE [H]

Synonym: Chy400_2237

Alternate gene names: 222525489

Gene position: 2829940-2829341 (Counterclockwise)

Preceding gene: 222525490

Following gene: 222525488

Centisome position: 53.71

GC content: 57.0

Gene sequence:

>600_bases
ATGCCAGACACTGAGGAAGTGAAACCTGAGACAACCACCGAAGCTGCTGCACCTCAGTCAGCAGACAAGGCTGTAGCCGA
AACTCCTGACACGACGGTAGAGGCTGCTACGTCTGAAGCCGCTGATCCGGCAGCCGTCATTGCCGATCTGCAAAACCGCC
TGGCGCAGGCCGAAGCCCAGGCTGCCGAGTACAAGGATCAGTGGATGCGAGCGGTCGCCGATTACCGCAATTTCAAGCGC
CGCACCGAAACTGAACGCACCGAGCTGGTGCGAAATGCTGGCGCAGCCCTGATCTTGAAGCTGCTGCCGGTGCTCGATGA
TTTCGAGCGCGCTATTGCCAATATTCCACCCGACATTGCCGAAACACCGTGGTGGCAGGGCACGCAACTCATTGCCCAGA
AATTGCGTACCATCCTCGAAAGTGAGGGAGTTAAGCCGATTGAGGCATTGGGGCAAGAGTTTAACCCCAACCTCCACGAA
GCGGTGATCTATGAAGATGCTGAGGGTCAGGAAGGTAAGGTCATCGCCGAATTGCAGCGTGGTTATCTGCTGCACGACCG
CGTGATCCGTCCGAGTATGGTCAAGGTAGGTCGCGGATAA

Upstream 100 bases:

>100_bases
TAATCTGCTGGCCGATCTGTACCACGTTAATACCCGTCCTTCTGATATAGAACCTGCCGCTGAACTATAGGCGGTGTATA
GTAGATAATGAGAGGAACCT

Downstream 100 bases:

>100_bases
AGTCGCCAAAAGCCTGTTTAAGGTAAAGGGTAGTTACCAATGCCAAAAGTGATTGGGATTGATCTGGGGACAACGAATTC
GGTGGTTGCAGTGGTTGAAG

Product: GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MPDTEEVKPETTTEAAAPQSADKAVAETPDTTVEAATSEAADPAAVIADLQNRLAQAEAQAAEYKDQWMRAVADYRNFKR
RTETERTELVRNAGAALILKLLPVLDDFERAIANIPPDIAETPWWQGTQLIAQKLRTILESEGVKPIEALGQEFNPNLHE
AVIYEDAEGQEGKVIAELQRGYLLHDRVIRPSMVKVGRG

Sequences:

>Translated_199_residues
MPDTEEVKPETTTEAAAPQSADKAVAETPDTTVEAATSEAADPAAVIADLQNRLAQAEAQAAEYKDQWMRAVADYRNFKR
RTETERTELVRNAGAALILKLLPVLDDFERAIANIPPDIAETPWWQGTQLIAQKLRTILESEGVKPIEALGQEFNPNLHE
AVIYEDAEGQEGKVIAELQRGYLLHDRVIRPSMVKVGRG
>Mature_198_residues
PDTEEVKPETTTEAAAPQSADKAVAETPDTTVEAATSEAADPAAVIADLQNRLAQAEAQAAEYKDQWMRAVADYRNFKRR
TETERTELVRNAGAALILKLLPVLDDFERAIANIPPDIAETPWWQGTQLIAQKLRTILESEGVKPIEALGQEFNPNLHEA
VIYEDAEGQEGKVIAELQRGYLLHDRVIRPSMVKVGRG

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=169, Percent_Identity=26.6272189349112, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1788967, Length=199, Percent_Identity=29.1457286432161, Blast_Score=72, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324806, Length=185, Percent_Identity=28.1081081081081, Blast_Score=73, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24653432, Length=171, Percent_Identity=26.9005847953216, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 21958; Mature: 21827

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDTEEVKPETTTEAAAPQSADKAVAETPDTTVEAATSEAADPAAVIADLQNRLAQAEAQ
CCCCCCCCCCCCHHHCCCCCHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
AAEYKDQWMRAVADYRNFKRRTETERTELVRNAGAALILKLLPVLDDFERAIANIPPDIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ETPWWQGTQLIAQKLRTILESEGVKPIEALGQEFNPNLHEAVIYEDAEGQEGKVIAELQR
CCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHEEECCCCCCCCHHHHHHHH
GYLLHDRVIRPSMVKVGRG
CHHHHHHHCCHHHHHCCCC
>Mature Secondary Structure 
PDTEEVKPETTTEAAAPQSADKAVAETPDTTVEAATSEAADPAAVIADLQNRLAQAEAQ
CCCCCCCCCCCHHHCCCCCHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
AAEYKDQWMRAVADYRNFKRRTETERTELVRNAGAALILKLLPVLDDFERAIANIPPDIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ETPWWQGTQLIAQKLRTILESEGVKPIEALGQEFNPNLHEAVIYEDAEGQEGKVIAELQR
CCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHEEECCCCCCCCHHHHHHHH
GYLLHDRVIRPSMVKVGRG
CHHHHHHHCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA