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The map label for this gene is lcfB [H]

Identifier: 222525456

GI number: 222525456

Start: 2793916

End: 2795418

Strand: Reverse

Name: lcfB [H]

Synonym: Chy400_2204

Alternate gene names: 222525456

Gene position: 2795418-2793916 (Counterclockwise)

Preceding gene: 222525457

Following gene: 222525455

Centisome position: 53.05

GC content: 58.02

Gene sequence:

>1503_bases
ATGTACCGTACCGTGTATGATCTCCTGACAACTGGAAGCGATACCGATCCTGCGCTGATTGCACCGGGTGGCCCGACCTG
GAACTTTGCCGATCTGCGTGCCCAGGTGTACCGTCTGGCCGAGTGGCTCCAGAACCATGGACTGGGACGCGGCGACCGGA
TTGCGATTGCGCTCGGCAACGGCCCGGCGATGGCGATCACCTTTCTGGCTGCGGCTACTGCGGCGACGGCTGCACCACTT
AATCCCAAATATCGGCGGGACGAATTTGCCTTCTACTATGAAGACACCCAGGCCCGCGCATTAATTGTTGCCCCTGGCGA
GGGCGACGAAGCCCGGGCTGCATTGCGTTCAGACATGCTGCTGATCGAAGCCGCCTTCGACAGCAACCATCAGCTTACCT
TCTCGACATCTGGTACCGCCGGATCACCACGCCGCGATGGCCTGGCCACACCCGACGACATCGCCATGATCTTGCACACC
AGTGGCACCACCAGCCGGCCTAAGCGGGTACCTATTCGCCATCGTAATCTGGCCGCCTCGACCAGCAACATCATCACTAC
CTATCAATTGAGCGCGGCGGATCGTTCGCTCTGCGTGATGCCGCTCTTCCACATTCACGGCATTGTTGCCTCGTTGCTAA
GCCAGTTAGCCGCCGGTGGAGCTGTTATCTGTCCACCAGGTTTTGATGGTCTCAAATTCTGGAGCTGGGTCGAACAAGAG
CGTCCAACCTGGTACTCGGCCGTCCCGACGATGCACCAGGTCCTCCTGGCCCGAGCCGGGCGCAACACTGCTATAATCAA
AGCTAACCCATTCCGCTTCATCCGCTCCTCAAGTGCACCTCTACCTCCGGTTGTGATGGAGCAAATGGAAGAGGTCTTCG
CGGCGCCGGTGATTGAAAGCTACGGCATGACGGAGGCTTCACATCAGATGACCTCGAATCCGTTACCACCCGGTCGGCGA
AAACCCGGCTCGGTGGGGATTGGATTCGGGGTAGAAGTGGGTATCATGGACGAACACGGCCAGTTGTTGCCGGCAGGTGT
GAAGGGCGAGGTCGTAGTGCGCGGGCCGAATGTCGTTGATGGGTACGAAAATAATCCTGAAGCCAATGCCACAGCATTTG
TGAATGGATGGTTTCGGACCGGTGACCAGGGGTATCTCGACGAAGATGGCTATCTCTGTCTGACCGGTCGTATCAAAGAG
TTAATCAACCGCGGGGGCGAAAAGATTTCGCCCCTGGAGATTGATGACGTACTGCTCCGTCATCCGGCAGTCGCCGAAGC
CCTGGCCTTTGCCGTGCCACATCCAACGCTGGGTGAAGAGGTGCATGCCGCCGTTGTATTACGCGAAGGCATGAGTGCTG
ACGAGCGTGAATTACGCGAGCACTGTGCCCGCCTGCTGGCCGATTTCAAAGTGCCTCGTGCAATCCATATTCTGAGCGCA
CTACCACGCGGTGCCACCGGAAAACTACAACGGATCACGATGGCGAAAACACTGGGATTGTAA

Upstream 100 bases:

>100_bases
GCTATGTAGCAGGTACTGAACCTCGTTGTGATGGGCTGGCAATTGGATGGTGATCATTACCGACGGCATACGAAACAATA
AACGATGGGGGATGAAGACG

Downstream 100 bases:

>100_bases
GCAACGGGAGAAAGACAGGAACCGGCATGATCCTGAAGTCAGAATTTGAACGCCGCCTTTCACCATACATGAGTTTTTCG
CGCTCAGAATGGGCAGCACT

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MYRTVYDLLTTGSDTDPALIAPGGPTWNFADLRAQVYRLAEWLQNHGLGRGDRIAIALGNGPAMAITFLAAATAATAAPL
NPKYRRDEFAFYYEDTQARALIVAPGEGDEARAALRSDMLLIEAAFDSNHQLTFSTSGTAGSPRRDGLATPDDIAMILHT
SGTTSRPKRVPIRHRNLAASTSNIITTYQLSAADRSLCVMPLFHIHGIVASLLSQLAAGGAVICPPGFDGLKFWSWVEQE
RPTWYSAVPTMHQVLLARAGRNTAIIKANPFRFIRSSSAPLPPVVMEQMEEVFAAPVIESYGMTEASHQMTSNPLPPGRR
KPGSVGIGFGVEVGIMDEHGQLLPAGVKGEVVVRGPNVVDGYENNPEANATAFVNGWFRTGDQGYLDEDGYLCLTGRIKE
LINRGGEKISPLEIDDVLLRHPAVAEALAFAVPHPTLGEEVHAAVVLREGMSADERELREHCARLLADFKVPRAIHILSA
LPRGATGKLQRITMAKTLGL

Sequences:

>Translated_500_residues
MYRTVYDLLTTGSDTDPALIAPGGPTWNFADLRAQVYRLAEWLQNHGLGRGDRIAIALGNGPAMAITFLAAATAATAAPL
NPKYRRDEFAFYYEDTQARALIVAPGEGDEARAALRSDMLLIEAAFDSNHQLTFSTSGTAGSPRRDGLATPDDIAMILHT
SGTTSRPKRVPIRHRNLAASTSNIITTYQLSAADRSLCVMPLFHIHGIVASLLSQLAAGGAVICPPGFDGLKFWSWVEQE
RPTWYSAVPTMHQVLLARAGRNTAIIKANPFRFIRSSSAPLPPVVMEQMEEVFAAPVIESYGMTEASHQMTSNPLPPGRR
KPGSVGIGFGVEVGIMDEHGQLLPAGVKGEVVVRGPNVVDGYENNPEANATAFVNGWFRTGDQGYLDEDGYLCLTGRIKE
LINRGGEKISPLEIDDVLLRHPAVAEALAFAVPHPTLGEEVHAAVVLREGMSADERELREHCARLLADFKVPRAIHILSA
LPRGATGKLQRITMAKTLGL
>Mature_500_residues
MYRTVYDLLTTGSDTDPALIAPGGPTWNFADLRAQVYRLAEWLQNHGLGRGDRIAIALGNGPAMAITFLAAATAATAAPL
NPKYRRDEFAFYYEDTQARALIVAPGEGDEARAALRSDMLLIEAAFDSNHQLTFSTSGTAGSPRRDGLATPDDIAMILHT
SGTTSRPKRVPIRHRNLAASTSNIITTYQLSAADRSLCVMPLFHIHGIVASLLSQLAAGGAVICPPGFDGLKFWSWVEQE
RPTWYSAVPTMHQVLLARAGRNTAIIKANPFRFIRSSSAPLPPVVMEQMEEVFAAPVIESYGMTEASHQMTSNPLPPGRR
KPGSVGIGFGVEVGIMDEHGQLLPAGVKGEVVVRGPNVVDGYENNPEANATAFVNGWFRTGDQGYLDEDGYLCLTGRIKE
LINRGGEKISPLEIDDVLLRHPAVAEALAFAVPHPTLGEEVHAAVVLREGMSADERELREHCARLLADFKVPRAIHILSA
LPRGATGKLQRITMAKTLGL

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=377, Percent_Identity=34.4827586206897, Blast_Score=192, Evalue=9e-49,
Organism=Homo sapiens, GI187761343, Length=377, Percent_Identity=34.4827586206897, Blast_Score=192, Evalue=9e-49,
Organism=Homo sapiens, GI156151445, Length=514, Percent_Identity=27.8210116731518, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI58082049, Length=491, Percent_Identity=27.0875763747454, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI157311624, Length=494, Percent_Identity=26.3157894736842, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI157311622, Length=494, Percent_Identity=26.3157894736842, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI115511026, Length=491, Percent_Identity=24.847250509165, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI42544132, Length=529, Percent_Identity=24.3856332703214, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI122937307, Length=505, Percent_Identity=25.5445544554455, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI38505220, Length=490, Percent_Identity=26.1224489795918, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI28416953, Length=524, Percent_Identity=22.9007633587786, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI8923896, Length=366, Percent_Identity=26.775956284153, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI253970489, Length=366, Percent_Identity=26.775956284153, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI13375727, Length=528, Percent_Identity=24.2424242424242, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI13236579, Length=492, Percent_Identity=25.2032520325203, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI195947378, Length=407, Percent_Identity=23.8329238329238, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI221142682, Length=508, Percent_Identity=28.5433070866142, Blast_Score=195, Evalue=7e-51,
Organism=Escherichia coli, GI145693145, Length=500, Percent_Identity=28.2, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI1786810, Length=518, Percent_Identity=26.4478764478764, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1788107, Length=510, Percent_Identity=26.4705882352941, Blast_Score=149, Evalue=6e-37,
Organism=Escherichia coli, GI1790505, Length=517, Percent_Identity=23.5976789168279, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1789201, Length=368, Percent_Identity=26.3586956521739, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1786801, Length=422, Percent_Identity=23.696682464455, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786529, Length=523, Percent_Identity=25.0478011472275, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788595, Length=457, Percent_Identity=23.8512035010941, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17558820, Length=492, Percent_Identity=29.8780487804878, Blast_Score=199, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI32563687, Length=498, Percent_Identity=29.5180722891566, Blast_Score=190, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI71994694, Length=545, Percent_Identity=27.3394495412844, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI71994703, Length=545, Percent_Identity=27.3394495412844, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI71994690, Length=545, Percent_Identity=27.3394495412844, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17557194, Length=493, Percent_Identity=26.369168356998, Blast_Score=141, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI17560140, Length=489, Percent_Identity=24.9488752556237, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17559526, Length=363, Percent_Identity=29.2011019283747, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17531443, Length=494, Percent_Identity=24.2914979757085, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17538037, Length=498, Percent_Identity=26.5060240963855, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17560308, Length=520, Percent_Identity=25.3846153846154, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71982997, Length=525, Percent_Identity=24.5714285714286, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI71983001, Length=525, Percent_Identity=24.5714285714286, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI71996755, Length=481, Percent_Identity=24.5322245322245, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71985884, Length=354, Percent_Identity=26.5536723163842, Blast_Score=86, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6319699, Length=484, Percent_Identity=43.801652892562, Blast_Score=398, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6319264, Length=543, Percent_Identity=23.3885819521179, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323182, Length=525, Percent_Identity=22.8571428571429, Blast_Score=90, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6320852, Length=202, Percent_Identity=27.7227722772277, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24581924, Length=544, Percent_Identity=28.125, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI18859661, Length=490, Percent_Identity=29.5918367346939, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI21355181, Length=501, Percent_Identity=25.748502994012, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24656500, Length=507, Percent_Identity=27.0216962524655, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19922652, Length=526, Percent_Identity=25.6653992395437, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI62472339, Length=528, Percent_Identity=24.0530303030303, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24667955, Length=527, Percent_Identity=24.0986717267552, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24648260, Length=475, Percent_Identity=26.5263157894737, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648253, Length=487, Percent_Identity=25.0513347022587, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21356947, Length=478, Percent_Identity=25.3138075313808, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24667959, Length=365, Percent_Identity=26.027397260274, Blast_Score=106, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24648255, Length=487, Percent_Identity=24.8459958932238, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI20130357, Length=491, Percent_Identity=23.4215885947047, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648257, Length=348, Percent_Identity=27.2988505747126, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI281365686, Length=487, Percent_Identity=23.8193018480493, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21356441, Length=501, Percent_Identity=23.9520958083832, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21358303, Length=477, Percent_Identity=24.1090146750524, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24653035, Length=492, Percent_Identity=25.4065040650407, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI221329755, Length=399, Percent_Identity=23.0576441102757, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI161076582, Length=495, Percent_Identity=24.4444444444444, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21355225, Length=368, Percent_Identity=24.1847826086957, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI19921316, Length=359, Percent_Identity=25.3481894150418, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 54026; Mature: 54026

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRTVYDLLTTGSDTDPALIAPGGPTWNFADLRAQVYRLAEWLQNHGLGRGDRIAIALGN
CCHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
GPAMAITFLAAATAATAAPLNPKYRRDEFAFYYEDTQARALIVAPGEGDEARAALRSDML
CCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCHHHHHHHHHCEE
LIEAAFDSNHQLTFSTSGTAGSPRRDGLATPDDIAMILHTSGTTSRPKRVPIRHRNLAAS
EEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCC
TSNIITTYQLSAADRSLCVMPLFHIHGIVASLLSQLAAGGAVICPPGFDGLKFWSWVEQE
CCCEEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHC
RPTWYSAVPTMHQVLLARAGRNTAIIKANPFRFIRSSSAPLPPVVMEQMEEVFAAPVIES
CCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHEECCCCCCCHHHHHHHHHHHHHHHHHH
YGMTEASHQMTSNPLPPGRRKPGSVGIGFGVEVGIMDEHGQLLPAGVKGEVVVRGPNVVD
CCCCHHHHHHCCCCCCCCCCCCCCEEECCCEEEEEECCCCCEEECCCCCCEEEECCCEEC
GYENNPEANATAFVNGWFRTGDQGYLDEDGYLCLTGRIKELINRGGEKISPLEIDDVLLR
CCCCCCCCCEEEEEEEEEECCCCCCCCCCCEEEECHHHHHHHHCCCCCCCCCCHHHHHHC
HPAVAEALAFAVPHPTLGEEVHAAVVLREGMSADERELREHCARLLADFKVPRAIHILSA
CCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
LPRGATGKLQRITMAKTLGL
CCCCCCCCHHEEEHHHHHCC
>Mature Secondary Structure
MYRTVYDLLTTGSDTDPALIAPGGPTWNFADLRAQVYRLAEWLQNHGLGRGDRIAIALGN
CCHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
GPAMAITFLAAATAATAAPLNPKYRRDEFAFYYEDTQARALIVAPGEGDEARAALRSDML
CCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCHHHHHHHHHCEE
LIEAAFDSNHQLTFSTSGTAGSPRRDGLATPDDIAMILHTSGTTSRPKRVPIRHRNLAAS
EEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCC
TSNIITTYQLSAADRSLCVMPLFHIHGIVASLLSQLAAGGAVICPPGFDGLKFWSWVEQE
CCCEEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHC
RPTWYSAVPTMHQVLLARAGRNTAIIKANPFRFIRSSSAPLPPVVMEQMEEVFAAPVIES
CCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHEECCCCCCCHHHHHHHHHHHHHHHHHH
YGMTEASHQMTSNPLPPGRRKPGSVGIGFGVEVGIMDEHGQLLPAGVKGEVVVRGPNVVD
CCCCHHHHHHCCCCCCCCCCCCCCEEECCCEEEEEECCCCCEEECCCCCCEEEECCCEEC
GYENNPEANATAFVNGWFRTGDQGYLDEDGYLCLTGRIKELINRGGEKISPLEIDDVLLR
CCCCCCCCCEEEEEEEEEECCCCCCCCCCCEEEECHHHHHHHHCCCCCCCCCCHHHHHHC
HPAVAEALAFAVPHPTLGEEVHAAVVLREGMSADERELREHCARLLADFKVPRAIHILSA
CCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
LPRGATGKLQRITMAKTLGL
CCCCCCCCHHEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]