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The map label for this gene is nagD [H]

Identifier: 222525441

GI number: 222525441

Start: 2770953

End: 2771759

Strand: Direct

Name: nagD [H]

Synonym: Chy400_2186

Alternate gene names: 222525441

Gene position: 2770953-2771759 (Clockwise)

Preceding gene: 222525440

Following gene: 222525442

Centisome position: 52.59

GC content: 60.47

Gene sequence:

>807_bases
ATGTTCTCCTTCAATACCATCCGCGCCGTTCTGTTCGATATGGACGGTGTGCTGTACCGGGGGCAGACGCCGTTGCCGGG
AGTTTCCGATCTGTTCCAGTTTCTCACCGAACAGCAGATTGCCTTTGCCTGTGCCACCAACAATGCCTCAATGACACCGC
AGCAATACGAGGCGAAGCTGGCAGCAATGGGGATTACGCTGCCGGCGGATCGGGTGATTACCTCGGCGCAGGCGACGGCT
CGGTATCTGCGCGACCAGTACCCCGCCGGTACCCGCGTCTTTGTGGTTGGGATGCAAGGGCTACGTGAAGCGCTCTTCGC
TGATGGCTACTTTGTCGAAGACGATCAATCCCCCGATCTGGTCGTGCAAGGGGCCGATTTTACGCTGACGTATGACCGCC
TCAAGCGGGCAACGCTGCATATCCGGCGTGGTGCACGCTTCATTTCCACCAACCCCGACCGCACCTTTCCCAGCGAAGAG
GGTCTCATTCCCGGCGCCGGTGCGGTTGCCGCAGCACTCAGTGCGGCAACCGATGTTACACCGCTCGTGATCGGCAAGCC
GTCGCCAACCATGTTTCTCATTGGTGCCACCTTGCTTGGCGCAACGCCGGCGCAGACGCTGGTCGTGGGTGATCGGCTCG
ATACCGATATTGCCGGTGCAATCGCCGCAAACATGCCTTCTGTTTTGGTCTTGACCGGCGTCAGTACGCTGGCTGAAGCG
ACAACCGGCCCCATCCGCCCCGACCTGATTGTCGCCGATCTGCCCGAACTGCTCGAGCGCTGGCAGGCGAGTTTGCGCAC
ATCTTGA

Upstream 100 bases:

>100_bases
ATACTTGCGATATGCTCAACAAAGAGATAACTTCTGTTCTGAACTGAGATGGTTTGCGAGACGCCAATATGCTCGCACTG
CTATCATGGAAAGGCTAAAG

Downstream 100 bases:

>100_bases
TATCAAGTCTGGTCGGCTCATGTTCAAACTCAGTGCAATGTAGTATATTCGCATGAGACCCGTCGTATCACAGGTGAGCC
ATGGCATTTCCGCTGAGTGA

Product: HAD-superfamily hydrolase

Products: 4-nitrophenol; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MFSFNTIRAVLFDMDGVLYRGQTPLPGVSDLFQFLTEQQIAFACATNNASMTPQQYEAKLAAMGITLPADRVITSAQATA
RYLRDQYPAGTRVFVVGMQGLREALFADGYFVEDDQSPDLVVQGADFTLTYDRLKRATLHIRRGARFISTNPDRTFPSEE
GLIPGAGAVAAALSAATDVTPLVIGKPSPTMFLIGATLLGATPAQTLVVGDRLDTDIAGAIAANMPSVLVLTGVSTLAEA
TTGPIRPDLIVADLPELLERWQASLRTS

Sequences:

>Translated_268_residues
MFSFNTIRAVLFDMDGVLYRGQTPLPGVSDLFQFLTEQQIAFACATNNASMTPQQYEAKLAAMGITLPADRVITSAQATA
RYLRDQYPAGTRVFVVGMQGLREALFADGYFVEDDQSPDLVVQGADFTLTYDRLKRATLHIRRGARFISTNPDRTFPSEE
GLIPGAGAVAAALSAATDVTPLVIGKPSPTMFLIGATLLGATPAQTLVVGDRLDTDIAGAIAANMPSVLVLTGVSTLAEA
TTGPIRPDLIVADLPELLERWQASLRTS
>Mature_268_residues
MFSFNTIRAVLFDMDGVLYRGQTPLPGVSDLFQFLTEQQIAFACATNNASMTPQQYEAKLAAMGITLPADRVITSAQATA
RYLRDQYPAGTRVFVVGMQGLREALFADGYFVEDDQSPDLVVQGADFTLTYDRLKRATLHIRRGARFISTNPDRTFPSEE
GLIPGAGAVAAALSAATDVTPLVIGKPSPTMFLIGATLLGATPAQTLVVGDRLDTDIAGAIAANMPSVLVLTGVSTLAEA
TTGPIRPDLIVADLPELLERWQASLRTS

Specific function: Unknown

COG id: COG0647

COG function: function code G; Predicted sugar phosphatases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. NagD family [H]

Homologues:

Organism=Homo sapiens, GI10092677, Length=276, Percent_Identity=35.5072463768116, Blast_Score=124, Evalue=6e-29,
Organism=Homo sapiens, GI108796653, Length=292, Percent_Identity=31.8493150684932, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI14149777, Length=239, Percent_Identity=27.1966527196653, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1786890, Length=252, Percent_Identity=32.9365079365079, Blast_Score=146, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17558880, Length=262, Percent_Identity=29.0076335877863, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17562458, Length=264, Percent_Identity=28.7878787878788, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17560956, Length=264, Percent_Identity=28.7878787878788, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI193210059, Length=260, Percent_Identity=31.9230769230769, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI86563050, Length=239, Percent_Identity=32.2175732217573, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17557870, Length=257, Percent_Identity=28.7937743190661, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71984613, Length=263, Percent_Identity=26.2357414448669, Blast_Score=77, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6319965, Length=235, Percent_Identity=29.3617021276596, Blast_Score=102, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24666141, Length=261, Percent_Identity=26.8199233716475, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24656326, Length=282, Percent_Identity=28.7234042553192, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24656330, Length=287, Percent_Identity=26.8292682926829, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI18859765, Length=277, Percent_Identity=27.7978339350181, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19920940, Length=233, Percent_Identity=26.1802575107296, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24666137, Length=299, Percent_Identity=28.0936454849498, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006357
- InterPro:   IPR006354
- InterPro:   IPR023215 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: 3.1.3.41

Molecular weight: Translated: 28626; Mature: 28626

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSFNTIRAVLFDMDGVLYRGQTPLPGVSDLFQFLTEQQIAFACATNNASMTPQQYEAKL
CCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHH
AAMGITLPADRVITSAQATARYLRDQYPAGTRVFVVGMQGLREALFADGYFVEDDQSPDL
HHEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCEEECCCCCCCE
VVQGADFTLTYDRLKRATLHIRRGARFISTNPDRTFPSEEGLIPGAGAVAAALSAATDVT
EEECCCEEEEHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
PLVIGKPSPTMFLIGATLLGATPAQTLVVGDRLDTDIAGAIAANMPSVLVLTGVSTLAEA
EEEEECCCCCEEEEEHHHHCCCCCCEEEECCCCCCHHHHHHHHCCCCEEEECCHHHHHHH
TTGPIRPDLIVADLPELLERWQASLRTS
CCCCCCCCEEHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFSFNTIRAVLFDMDGVLYRGQTPLPGVSDLFQFLTEQQIAFACATNNASMTPQQYEAKL
CCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHH
AAMGITLPADRVITSAQATARYLRDQYPAGTRVFVVGMQGLREALFADGYFVEDDQSPDL
HHEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCEEECCCCCCCE
VVQGADFTLTYDRLKRATLHIRRGARFISTNPDRTFPSEEGLIPGAGAVAAALSAATDVT
EEECCCEEEEHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
PLVIGKPSPTMFLIGATLLGATPAQTLVVGDRLDTDIAGAIAANMPSVLVLTGVSTLAEA
EEEEECCCCCEEEEEHHHHCCCCCCEEEECCCCCCHHHHHHHHCCCCEEEECCHHHHHHH
TTGPIRPDLIVADLPELLERWQASLRTS
CCCCCCCCEEHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-nitrophenyl phosphate; H2O

Specific reaction: 4-nitrophenyl phosphate + H2O = 4-nitrophenol + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA