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The map label for this gene is rfbD [H]

Identifier: 222525379

GI number: 222525379

Start: 2698959

End: 2699936

Strand: Reverse

Name: rfbD [H]

Synonym: Chy400_2124

Alternate gene names: 222525379

Gene position: 2699936-2698959 (Counterclockwise)

Preceding gene: 222525380

Following gene: 222525378

Centisome position: 51.24

GC content: 58.08

Gene sequence:

>978_bases
ATGCGGGTCTTTATTACCGGTATTACCGGGCCGGTCGGCAGTTTTCTGGCCGATTATCTGTTGACAATACCTGGCGTGGA
CATTCACGCCTTCAAGCGCTGGCGTAGTGATCCGCGTCCAATTGAACATTTGATCGGACGGATCACCATTCATGAGGGCG
ATATTGAAGATGCGTTTGCGATAGATCGGGCCATTGCCACCGCACGCCCAGATCGCATCTTTCACCTCGCAGCGCAGAGC
TACCCCAGTGCTTCCTGGGACGCGCCGATTCTGACGATGCGGGCTAATGTGGAAGGCACCATCAACCTGCTTGAAGCAGC
GCGCCGGCACGTGCCGAAGGCGCGTATTCATATTGCCGGTACCAGTGCCGAATATGGGCCGGTTCGCCCTGATGAGGTGC
CGATCAGCGAGGACCATCCCTTACGACCGGCCAGTCCATACGGGGTGAGCAAAGTTGCTGCTGAATTGAGTGGCCTGCAA
TACCACGCCAGTTACGGGTTGCACGTGGTGGTGACTCGTTCGTTTAACCATGTCGGCCCCCGCCAGGGAGATCGTTGCTC
GATCCAAACCTTCTGTCGCCAGATGGCGTTAATCGAGGCCGGGCAGCAGGAACCGGTTATGTATGTCGGCAATCTGTCAC
CCAAACGTGATTTCACCCATACCCGCGATGTTGCCCGGGCACTCTGGCTACTGCTGGAACACGGTACGCCGGGTGAGGTG
TATAACCTCTGTTCGGGGCAGGCGGTACGCATCGGTGATATTGTGGATATGGTGATTGCAATGGGGCGCGTACCGGTGAC
CGTACAGGTCGATCCGGCCCGCCTGCGCCCGGTCGATGAGCCGATCCTCCAGGGAGACAACCGCAAACTGCGCGCAGCTA
CCGGCTGGCAACCCGAAATCGGGATCGAGCAGATCGTGGCTGAAGTGCTTGATTACTGGCGTCAACAGATAGGCAGCAAC
CAGCGCACGCAGATTTGA

Upstream 100 bases:

>100_bases
CTTCGCCCCCACAACCCGCACAACCTGTTCAGATCGTCTCCTGCGCCTGCCTGCCGACCAAACGTGGTATGATAGGCGAC
GGCTGTAACGAGGAGGTGTT

Downstream 100 bases:

>100_bases
TGTATGGGAACACTCTACCTTGTTGCCACACCAATCGGCAATCTCGAAGACATCACGCTCCGCGCATTACGCATCCTGCG
CGAGGTACGTCTGATTGCTG

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MRVFITGITGPVGSFLADYLLTIPGVDIHAFKRWRSDPRPIEHLIGRITIHEGDIEDAFAIDRAIATARPDRIFHLAAQS
YPSASWDAPILTMRANVEGTINLLEAARRHVPKARIHIAGTSAEYGPVRPDEVPISEDHPLRPASPYGVSKVAAELSGLQ
YHASYGLHVVVTRSFNHVGPRQGDRCSIQTFCRQMALIEAGQQEPVMYVGNLSPKRDFTHTRDVARALWLLLEHGTPGEV
YNLCSGQAVRIGDIVDMVIAMGRVPVTVQVDPARLRPVDEPILQGDNRKLRAATGWQPEIGIEQIVAEVLDYWRQQIGSN
QRTQI

Sequences:

>Translated_325_residues
MRVFITGITGPVGSFLADYLLTIPGVDIHAFKRWRSDPRPIEHLIGRITIHEGDIEDAFAIDRAIATARPDRIFHLAAQS
YPSASWDAPILTMRANVEGTINLLEAARRHVPKARIHIAGTSAEYGPVRPDEVPISEDHPLRPASPYGVSKVAAELSGLQ
YHASYGLHVVVTRSFNHVGPRQGDRCSIQTFCRQMALIEAGQQEPVMYVGNLSPKRDFTHTRDVARALWLLLEHGTPGEV
YNLCSGQAVRIGDIVDMVIAMGRVPVTVQVDPARLRPVDEPILQGDNRKLRAATGWQPEIGIEQIVAEVLDYWRQQIGSN
QRTQI
>Mature_325_residues
MRVFITGITGPVGSFLADYLLTIPGVDIHAFKRWRSDPRPIEHLIGRITIHEGDIEDAFAIDRAIATARPDRIFHLAAQS
YPSASWDAPILTMRANVEGTINLLEAARRHVPKARIHIAGTSAEYGPVRPDEVPISEDHPLRPASPYGVSKVAAELSGLQ
YHASYGLHVVVTRSFNHVGPRQGDRCSIQTFCRQMALIEAGQQEPVMYVGNLSPKRDFTHTRDVARALWLLLEHGTPGEV
YNLCSGQAVRIGDIVDMVIAMGRVPVTVQVDPARLRPVDEPILQGDNRKLRAATGWQPEIGIEQIVAEVLDYWRQQIGSN
QRTQI

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=338, Percent_Identity=30.4733727810651, Blast_Score=154, Evalue=9e-38,
Organism=Homo sapiens, GI42516563, Length=338, Percent_Identity=27.2189349112426, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI7657641, Length=271, Percent_Identity=25.830258302583, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1788366, Length=347, Percent_Identity=30.2593659942363, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1788353, Length=341, Percent_Identity=26.099706744868, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI48994969, Length=355, Percent_Identity=27.3239436619718, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1786974, Length=342, Percent_Identity=26.6081871345029, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI133901788, Length=337, Percent_Identity=32.0474777448071, Blast_Score=165, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17539424, Length=337, Percent_Identity=32.0474777448071, Blast_Score=165, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17539422, Length=337, Percent_Identity=32.0474777448071, Blast_Score=165, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI133901786, Length=337, Percent_Identity=32.0474777448071, Blast_Score=165, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI133901790, Length=337, Percent_Identity=32.0474777448071, Blast_Score=165, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17507723, Length=337, Percent_Identity=34.1246290801187, Blast_Score=164, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI17539532, Length=338, Percent_Identity=27.2189349112426, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17568069, Length=347, Percent_Identity=25.9365994236311, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24158427, Length=338, Percent_Identity=29.2899408284024, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI21356223, Length=336, Percent_Identity=26.1904761904762, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 36061; Mature: 36061

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVFITGITGPVGSFLADYLLTIPGVDIHAFKRWRSDPRPIEHLIGRITIHEGDIEDAFA
CEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHCEEEEECCCCHHHHH
IDRAIATARPDRIFHLAAQSYPSASWDAPILTMRANVEGTINLLEAARRHVPKARIHIAG
HHHHHHHCCCCCEEEEHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEEEE
TSAEYGPVRPDEVPISEDHPLRPASPYGVSKVAAELSGLQYHASYGLHVVVTRSFNHVGP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEECCCCEEEEEEECCCCCCC
RQGDRCSIQTFCRQMALIEAGQQEPVMYVGNLSPKRDFTHTRDVARALWLLLEHGTPGEV
CCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHH
YNLCSGQAVRIGDIVDMVIAMGRVPVTVQVDPARLRPVDEPILQGDNRKLRAATGWQPEI
HHHCCCCEEEHHHHHHHHHHHCCCCEEEEECHHHCCCCCHHHHCCCCCEEEEECCCCCCC
GIEQIVAEVLDYWRQQIGSNQRTQI
CHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRVFITGITGPVGSFLADYLLTIPGVDIHAFKRWRSDPRPIEHLIGRITIHEGDIEDAFA
CEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHCEEEEECCCCHHHHH
IDRAIATARPDRIFHLAAQSYPSASWDAPILTMRANVEGTINLLEAARRHVPKARIHIAG
HHHHHHHCCCCCEEEEHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEEEE
TSAEYGPVRPDEVPISEDHPLRPASPYGVSKVAAELSGLQYHASYGLHVVVTRSFNHVGP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEECCCCEEEEEEECCCCCCC
RQGDRCSIQTFCRQMALIEAGQQEPVMYVGNLSPKRDFTHTRDVARALWLLLEHGTPGEV
CCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHH
YNLCSGQAVRIGDIVDMVIAMGRVPVTVQVDPARLRPVDEPILQGDNRKLRAATGWQPEI
HHHCCCCEEEHHHHHHHHHHHCCCCEEEEECHHHCCCCCHHHHCCCCCEEEEECCCCCCC
GIEQIVAEVLDYWRQQIGSNQRTQI
CHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1372980; 7540582; 10952301 [H]