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The map label for this gene is hisF [H]

Identifier: 222525238

GI number: 222525238

Start: 2510665

End: 2511444

Strand: Direct

Name: hisF [H]

Synonym: Chy400_1978

Alternate gene names: 222525238

Gene position: 2510665-2511444 (Clockwise)

Preceding gene: 222525237

Following gene: 222525242

Centisome position: 47.65

GC content: 59.74

Gene sequence:

>780_bases
ATGCTGACGCGACGAATCATTCCCTGTCTTGATGTCAAAGCAGGACGTGTGGTGAAGGGCGTGAAATTCCTGAATCACCG
TGATGCCGGTGATCCGGTAGAGCTGGCGGCTGCCTACAATGCTGCCGGGGCCGATGAACTGGTCTTCTACGACATCACGG
CATCCAGCGACGAGCGGGCCATTATGGTCGAGGTTGTAGAACGCACGGCTGCCGAAGTATTTATTCCGCTCACGGTTGGT
GGTGGGTTACGCAGCGTTGACGATATGTACCGCATGCTGCGGGCCGGTGCCGACAAGGTCAGCCTGAATACAGCAGCGGT
CTACAACCCGCAGCTCATTGCCGAGGGCGCCCGGCGGTTTGGCAGCCAATGCATTGTGCTGTCGGTTGATGCCAAACGGG
TGAACGCGCCAGGCGAACCACCACGCTGGGAAGTCTTTACCCATACCGGGGCCAATCCGCGCCCGACGGGGCTTGACGCT
ATCGAGTGGATCAAGCGAGGGATCGATCTGGGGGCCGGTGAAATCTGTATCAATAGCATGGACGCCGACGGTGCGCGTAC
CGGGTATGATCTGGAGCTATTACAGGCGATTACCGCCATTTCCCCGGTACCGGTCATCGCGTCCGGTGGTGTCGGTTCGC
CGCACGACATGTATCGCGGCATCGTCGAAGGCGGTGCCGATGCGGTACTGGCCGCGTCCATCTTCCACTTCGGCGACTAC
TCAGTGGCCGATGTCAAACGCTACCTGGCTGAACGGGGGGTGGCGGTGAGGATGATATAA

Upstream 100 bases:

>100_bases
GATGCGTACAGGAAGGTGGTCGCGCACAACAACGACCACCAACTCAGCGATGGTTTACAGGCCATTACTGCGATTTCCGA
ACGTTACCGGGGACGAACAT

Downstream 100 bases:

>100_bases
GGAAGTTTACCTGCTTCTACCCACCCAATTCCTCATCCTCATCCACGTGCTGCACCCGTAGTTGCCAGAGCAAACCGCCG
AGCACCGGGCCAAAGCGATC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MLTRRIIPCLDVKAGRVVKGVKFLNHRDAGDPVELAAAYNAAGADELVFYDITASSDERAIMVEVVERTAAEVFIPLTVG
GGLRSVDDMYRMLRAGADKVSLNTAAVYNPQLIAEGARRFGSQCIVLSVDAKRVNAPGEPPRWEVFTHTGANPRPTGLDA
IEWIKRGIDLGAGEICINSMDADGARTGYDLELLQAITAISPVPVIASGGVGSPHDMYRGIVEGGADAVLAASIFHFGDY
SVADVKRYLAERGVAVRMI

Sequences:

>Translated_259_residues
MLTRRIIPCLDVKAGRVVKGVKFLNHRDAGDPVELAAAYNAAGADELVFYDITASSDERAIMVEVVERTAAEVFIPLTVG
GGLRSVDDMYRMLRAGADKVSLNTAAVYNPQLIAEGARRFGSQCIVLSVDAKRVNAPGEPPRWEVFTHTGANPRPTGLDA
IEWIKRGIDLGAGEICINSMDADGARTGYDLELLQAITAISPVPVIASGGVGSPHDMYRGIVEGGADAVLAASIFHFGDY
SVADVKRYLAERGVAVRMI
>Mature_259_residues
MLTRRIIPCLDVKAGRVVKGVKFLNHRDAGDPVELAAAYNAAGADELVFYDITASSDERAIMVEVVERTAAEVFIPLTVG
GGLRSVDDMYRMLRAGADKVSLNTAAVYNPQLIAEGARRFGSQCIVLSVDAKRVNAPGEPPRWEVFTHTGANPRPTGLDA
IEWIKRGIDLGAGEICINSMDADGARTGYDLELLQAITAISPVPVIASGGVGSPHDMYRGIVEGGADAVLAASIFHFGDY
SVADVKRYLAERGVAVRMI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=260, Percent_Identity=44.6153846153846, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI87082028, Length=216, Percent_Identity=27.3148148148148, Blast_Score=76, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319725, Length=315, Percent_Identity=36.1904761904762, Blast_Score=164, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27644; Mature: 27644

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRRIIPCLDVKAGRVVKGVKFLNHRDAGDPVELAAAYNAAGADELVFYDITASSDERA
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCC
IMVEVVERTAAEVFIPLTVGGGLRSVDDMYRMLRAGADKVSLNTAAVYNPQLIAEGARRF
HHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHCCCCEEECCEEEEECHHHHHHHHHHH
GSQCIVLSVDAKRVNAPGEPPRWEVFTHTGANPRPTGLDAIEWIKRGIDLGAGEICINSM
CCEEEEEEECHHCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCEEEECC
DADGARTGYDLELLQAITAISPVPVIASGGVGSPHDMYRGIVEGGADAVLAASIFHFGDY
CCCCCCCCCCHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCC
SVADVKRYLAERGVAVRMI
CHHHHHHHHHHCCEEEEEC
>Mature Secondary Structure
MLTRRIIPCLDVKAGRVVKGVKFLNHRDAGDPVELAAAYNAAGADELVFYDITASSDERA
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCC
IMVEVVERTAAEVFIPLTVGGGLRSVDDMYRMLRAGADKVSLNTAAVYNPQLIAEGARRF
HHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHCCCCEEECCEEEEECHHHHHHHHHHH
GSQCIVLSVDAKRVNAPGEPPRWEVFTHTGANPRPTGLDAIEWIKRGIDLGAGEICINSM
CCEEEEEEECHHCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCEEEECC
DADGARTGYDLELLQAITAISPVPVIASGGVGSPHDMYRGIVEGGADAVLAASIFHFGDY
CCCCCCCCCCHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCC
SVADVKRYLAERGVAVRMI
CHHHHHHHHHHCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA