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The map label for this gene is estA [H]

Identifier: 222525171

GI number: 222525171

Start: 2414105

End: 2414773

Strand: Reverse

Name: estA [H]

Synonym: Chy400_1909

Alternate gene names: 222525171

Gene position: 2414773-2414105 (Counterclockwise)

Preceding gene: 222525172

Following gene: 222525170

Centisome position: 45.83

GC content: 59.64

Gene sequence:

>669_bases
ATGACAACAATTCGTACTATTGAACGCAGGCCAATTATCGAGAGCGGACCGGCACCGTTGTTACTGATGCTGCACGGTTT
CGGGAGTCACGAACGCGATCTCTTCGAGCTTGCCGATCTGATTGACGACCGGATGCATATCGTGAGTGCCCGGGCACCGA
TTGCGTTGCCGTGGGGCGGCTTTGCCTGGTATGAGCTAAGTGGTACACCCGGTCGGCTCGTTCCCGATCCGGTGGGTCGG
GCGCAGGCCATCGAGTTGCTGATCAAGTTTGTCAGCGAATTGCCTGGCCGGATCGGAACTGATCCGCGGCGAACCTATCT
GTTCGGCTTTAGCCAGGGGGCGATTCTCAGTATGGCGCTGGCCTGGCGCATTCCCGAACACCTGGCCGGCGTGATTGCAG
CGAACGGCTACCTTGATCCGGCCCTGACCACACAACCACCCGCTGCCGGCATTGCCCGTCTGCCGATCTTGCAATTGCAC
GGCACCTACGATGAAGTCATTCCGGTTGAACAGGCACGAGCGACCCGTGATGTGCTGGCCCAATACGCACCACGCCATCG
CTATCATGAAGACCCGGTAGGACACAGTCTGCATCCAAATGGCTTGAGCCTGATGCAGCACTGGTTGGCCGAACAGCTCG
ACGCCCCGCCACCGCACGATCTCGCGTAG

Upstream 100 bases:

>100_bases
CTGAGCAATTTGGGGTTACAATTGAGGCACTCTTGCAAGCCAATAATCTCACTCCAGCCCAGGGGGATAACCTGCAAGTT
GGACAGGAACTGGTGATTCC

Downstream 100 bases:

>100_bases
GCAGGTAGGCTATGGCCGCTGATCATGTGCATTGTGCCAGTTGTGGACAGCCGCTTACTGGTAGTTATTATGTCTTGATC
AACCGGCCAGAACGGTTTTG

Product: phospholipase/carboxylesterase

Products: NA

Alternate protein names: Esterase I [H]

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MTTIRTIERRPIIESGPAPLLLMLHGFGSHERDLFELADLIDDRMHIVSARAPIALPWGGFAWYELSGTPGRLVPDPVGR
AQAIELLIKFVSELPGRIGTDPRRTYLFGFSQGAILSMALAWRIPEHLAGVIAANGYLDPALTTQPPAAGIARLPILQLH
GTYDEVIPVEQARATRDVLAQYAPRHRYHEDPVGHSLHPNGLSLMQHWLAEQLDAPPPHDLA

Sequences:

>Translated_222_residues
MTTIRTIERRPIIESGPAPLLLMLHGFGSHERDLFELADLIDDRMHIVSARAPIALPWGGFAWYELSGTPGRLVPDPVGR
AQAIELLIKFVSELPGRIGTDPRRTYLFGFSQGAILSMALAWRIPEHLAGVIAANGYLDPALTTQPPAAGIARLPILQLH
GTYDEVIPVEQARATRDVLAQYAPRHRYHEDPVGHSLHPNGLSLMQHWLAEQLDAPPPHDLA
>Mature_221_residues
TTIRTIERRPIIESGPAPLLLMLHGFGSHERDLFELADLIDDRMHIVSARAPIALPWGGFAWYELSGTPGRLVPDPVGRA
QAIELLIKFVSELPGRIGTDPRRTYLFGFSQGAILSMALAWRIPEHLAGVIAANGYLDPALTTQPPAAGIARLPILQLHG
TYDEVIPVEQARATRDVLAQYAPRHRYHEDPVGHSLHPNGLSLMQHWLAEQLDAPPPHDLA

Specific function: Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 24476; Mature: 24345

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIRTIERRPIIESGPAPLLLMLHGFGSHERDLFELADLIDDRMHIVSARAPIALPWGG
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCC
FAWYELSGTPGRLVPDPVGRAQAIELLIKFVSELPGRIGTDPRRTYLFGFSQGAILSMAL
EEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHH
AWRIPEHLAGVIAANGYLDPALTTQPPAAGIARLPILQLHGTYDEVIPVEQARATRDVLA
HHHCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCEEEECCCHHHHCCHHHHHHHHHHHH
QYAPRHRYHEDPVGHSLHPNGLSLMQHWLAEQLDAPPPHDLA
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TTIRTIERRPIIESGPAPLLLMLHGFGSHERDLFELADLIDDRMHIVSARAPIALPWGG
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCC
FAWYELSGTPGRLVPDPVGRAQAIELLIKFVSELPGRIGTDPRRTYLFGFSQGAILSMAL
EEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHH
AWRIPEHLAGVIAANGYLDPALTTQPPAAGIARLPILQLHGTYDEVIPVEQARATRDVLA
HHHCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCEEEECCCHHHHCCHHHHHHHHHHHH
QYAPRHRYHEDPVGHSLHPNGLSLMQHWLAEQLDAPPPHDLA
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA