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The map label for this gene is gabT [C]

Identifier: 222525123

GI number: 222525123

Start: 2351756

End: 2353105

Strand: Reverse

Name: gabT [C]

Synonym: Chy400_1861

Alternate gene names: 222525123

Gene position: 2353105-2351756 (Counterclockwise)

Preceding gene: 222525127

Following gene: 222525122

Centisome position: 44.66

GC content: 58.44

Gene sequence:

>1350_bases
ATGACCACACACACTGAAACGGCACGGTTGCCAACCGATTACATGCCGGGCCCGGTTCCTGGCCCACGAGCGCAGGAGCT
GGTGGCTCGCGATCACACTGTAATTGCCCCACTCGGACGGGTGTACCCCCTGGTGATTGACCATGCGCAGGGCTGCGAGG
TCTGGGATGTCGATGGCAATCGCTTCCTCGATATGAATGCAGGCATTGCAGTTTTGGCTGCCGGTCATTGCCATCCGCGT
CTGATCCAGGTTGCCCAGGAACAGCTTCAACGCTTCACCCACATGGCAGGCACCGACTTCTACAACGAACCAATGGTGCG
CGCTGCCGAGAAGCTGGTCTCACTGATGCCCGGCGGCAAGGACTGGCAGGTTTTTTTCACGAACAGTGGCACGGAAGCAG
TTGAAGCCAGTATTAAACTGGCCCGTTATGCAACCGGTCGCCAGAACATTATTGCCTTTTACGGCGCATTTCACGGTCGC
AGCTACGGCAGCCTTTCGCTCACCGCGTCCAAACCACGCCAGCGCCAGGGGTTCTTCCCGCTGCTACCCGGCGTCAGCCA
TACCTACTATCCCAATTGTTACCGCTGCCCGATCAATCTTCGTTTTCCAGAGTGTGACATTGCCTGTCTTGATCTGATCG
AGCGCACGTTGTTCAAGACCACAACCCCCCCGGAAGAAGTTGCCGCCATCATTATCGAGCCGATCCAGGGTGAGGGTGGC
TATGTAGTGCCGCCTCCCGGAGCGCTGGCTCGTCTGCGCTCGATCTGTGATCGTTACGGCATTCTGCTCATCGTTGATGA
GGTGCAGAGTGGTGTGGGTCGTACCGGTAAGATGTGGGCTTTTGAACACGAGGGTATTGTGCCCGATATTGTGGCTTCGG
CCAAGGGACTGGGCGGTGGTTTACCGCTGGGTGCCATGATCGCCCGCAGCGAGCTGGCCCGCAAATGGCAACCCGGTTCG
CATGGCAATACGTATGGCGGGAATGGTCTGACCTGTGCGGTCGCGTATGAACTGCTCACGATGGTTGAAGAGGAGTTGAT
GGACAATGCCGCCACGGTTGGTGCCTATCTCCTCGAACAACTGCAAGCCCTTCAGCAGCGATTCCCGCAGATCGGAGCAG
TACGCGGGCGCGGCCTGATGATCGGTGTCGAGTTTGTCGATAGTCAGGGCAATCCCGCCGGTGCGCTGGCCGAGGCAGTG
ATGGTCGAGAGTTTTCGCAAAGGACTGCTGGTACTCACCTGCGGTGCCTCAACAATCCGCTTCTGCCCACCATTGACCCT
GACGAAAGCTCAGGCCGACGAGGCCGTAGCCCGGTTTGCACTGGCAATTGAAGCCTGTCTGACTGCGTAG

Upstream 100 bases:

>100_bases
CGCCTGAACTATACCGCCATTGACAATGCTTGACGCCTGATCTGCACGTCGGCTACAATGATGACCCTGACAATTGGAGT
TAGCCGCAAGGGAGCACATC

Downstream 100 bases:

>100_bases
CCTGGGCAGAAGGAGGGCAACCCGCGGTACATGTGGGTTGCCCCGTATGCAAAAATAAGCTGGTGGCAACGCCCTCATCC
CGGGTTAGATGATGCACATT

Product: class III aminotransferase

Products: succinate semialdehyde; L-glutamate

Alternate protein names: ORF B [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MTTHTETARLPTDYMPGPVPGPRAQELVARDHTVIAPLGRVYPLVIDHAQGCEVWDVDGNRFLDMNAGIAVLAAGHCHPR
LIQVAQEQLQRFTHMAGTDFYNEPMVRAAEKLVSLMPGGKDWQVFFTNSGTEAVEASIKLARYATGRQNIIAFYGAFHGR
SYGSLSLTASKPRQRQGFFPLLPGVSHTYYPNCYRCPINLRFPECDIACLDLIERTLFKTTTPPEEVAAIIIEPIQGEGG
YVVPPPGALARLRSICDRYGILLIVDEVQSGVGRTGKMWAFEHEGIVPDIVASAKGLGGGLPLGAMIARSELARKWQPGS
HGNTYGGNGLTCAVAYELLTMVEEELMDNAATVGAYLLEQLQALQQRFPQIGAVRGRGLMIGVEFVDSQGNPAGALAEAV
MVESFRKGLLVLTCGASTIRFCPPLTLTKAQADEAVARFALAIEACLTA

Sequences:

>Translated_449_residues
MTTHTETARLPTDYMPGPVPGPRAQELVARDHTVIAPLGRVYPLVIDHAQGCEVWDVDGNRFLDMNAGIAVLAAGHCHPR
LIQVAQEQLQRFTHMAGTDFYNEPMVRAAEKLVSLMPGGKDWQVFFTNSGTEAVEASIKLARYATGRQNIIAFYGAFHGR
SYGSLSLTASKPRQRQGFFPLLPGVSHTYYPNCYRCPINLRFPECDIACLDLIERTLFKTTTPPEEVAAIIIEPIQGEGG
YVVPPPGALARLRSICDRYGILLIVDEVQSGVGRTGKMWAFEHEGIVPDIVASAKGLGGGLPLGAMIARSELARKWQPGS
HGNTYGGNGLTCAVAYELLTMVEEELMDNAATVGAYLLEQLQALQQRFPQIGAVRGRGLMIGVEFVDSQGNPAGALAEAV
MVESFRKGLLVLTCGASTIRFCPPLTLTKAQADEAVARFALAIEACLTA
>Mature_448_residues
TTHTETARLPTDYMPGPVPGPRAQELVARDHTVIAPLGRVYPLVIDHAQGCEVWDVDGNRFLDMNAGIAVLAAGHCHPRL
IQVAQEQLQRFTHMAGTDFYNEPMVRAAEKLVSLMPGGKDWQVFFTNSGTEAVEASIKLARYATGRQNIIAFYGAFHGRS
YGSLSLTASKPRQRQGFFPLLPGVSHTYYPNCYRCPINLRFPECDIACLDLIERTLFKTTTPPEEVAAIIIEPIQGEGGY
VVPPPGALARLRSICDRYGILLIVDEVQSGVGRTGKMWAFEHEGIVPDIVASAKGLGGGLPLGAMIARSELARKWQPGSH
GNTYGGNGLTCAVAYELLTMVEEELMDNAATVGAYLLEQLQALQQRFPQIGAVRGRGLMIGVEFVDSQGNPAGALAEAVM
VESFRKGLLVLTCGASTIRFCPPLTLTKAQADEAVARFALAIEACLTA

Specific function: 4-aminobutyrate (GABA) degradation. [C]

COG id: COG0160

COG function: function code E; 4-aminobutyrate aminotransferase and related aminotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI13994255, Length=477, Percent_Identity=33.1236897274633, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI4557809, Length=425, Percent_Identity=31.5294117647059, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI24119277, Length=441, Percent_Identity=29.4784580498866, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI37574042, Length=412, Percent_Identity=30.3398058252427, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI226442709, Length=368, Percent_Identity=30.7065217391304, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI226442705, Length=412, Percent_Identity=29.6116504854369, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI284507298, Length=319, Percent_Identity=34.4827586206897, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI188536080, Length=460, Percent_Identity=28.0434782608696, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI38679950, Length=460, Percent_Identity=28.0434782608696, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI38679946, Length=460, Percent_Identity=28.0434782608696, Blast_Score=151, Evalue=1e-36,
Organism=Escherichia coli, GI1789016, Length=413, Percent_Identity=38.9830508474576, Blast_Score=289, Evalue=2e-79,
Organism=Escherichia coli, GI1787560, Length=393, Percent_Identity=42.7480916030534, Blast_Score=278, Evalue=7e-76,
Organism=Escherichia coli, GI1788044, Length=410, Percent_Identity=36.5853658536585, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI1789759, Length=423, Percent_Identity=35.4609929078014, Blast_Score=249, Evalue=4e-67,
Organism=Escherichia coli, GI145693181, Length=399, Percent_Identity=38.5964912280702, Blast_Score=238, Evalue=5e-64,
Organism=Escherichia coli, GI1786349, Length=379, Percent_Identity=31.1345646437995, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1786991, Length=334, Percent_Identity=32.6347305389222, Blast_Score=138, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI32564660, Length=424, Percent_Identity=36.3207547169811, Blast_Score=242, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI25144271, Length=422, Percent_Identity=32.4644549763033, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI25144274, Length=281, Percent_Identity=34.5195729537367, Blast_Score=158, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI71992977, Length=443, Percent_Identity=28.6681715575621, Blast_Score=157, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI17541228, Length=463, Percent_Identity=28.9416846652268, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI71981843, Length=177, Percent_Identity=29.3785310734463, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324432, Length=425, Percent_Identity=34.5882352941176, Blast_Score=228, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6323470, Length=417, Percent_Identity=32.8537170263789, Blast_Score=206, Evalue=8e-54,
Organism=Saccharomyces cerevisiae, GI6321456, Length=464, Percent_Identity=28.6637931034483, Blast_Score=143, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6324386, Length=350, Percent_Identity=25.7142857142857, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI28574759, Length=435, Percent_Identity=38.6206896551724, Blast_Score=241, Evalue=9e-64,
Organism=Drosophila melanogaster, GI161085790, Length=371, Percent_Identity=40.1617250673854, Blast_Score=231, Evalue=1e-60,
Organism=Drosophila melanogaster, GI21356575, Length=423, Percent_Identity=34.51536643026, Blast_Score=202, Evalue=5e-52,
Organism=Drosophila melanogaster, GI21357415, Length=426, Percent_Identity=30.7511737089202, Blast_Score=196, Evalue=3e-50,
Organism=Drosophila melanogaster, GI281366494, Length=466, Percent_Identity=27.4678111587983, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24667139, Length=466, Percent_Identity=27.4678111587983, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24667143, Length=466, Percent_Identity=27.4678111587983, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.19

Molecular weight: Translated: 48559; Mature: 48427

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTHTETARLPTDYMPGPVPGPRAQELVARDHTVIAPLGRVYPLVIDHAQGCEVWDVDGN
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEECHHHHHHHEEECCCCCEEEECCCC
RFLDMNAGIAVLAAGHCHPRLIQVAQEQLQRFTHMAGTDFYNEPMVRAAEKLVSLMPGGK
EEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
DWQVFFTNSGTEAVEASIKLARYATGRQNIIAFYGAFHGRSYGSLSLTASKPRQRQGFFP
CEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEHHCCCCCCCEEEEECCCHHHCCCCC
LLPGVSHTYYPNCYRCPINLRFPECDIACLDLIERTLFKTTTPPEEVAAIIIEPIQGEGG
CCCCCCCCCCCCEEECCEECCCCCCCHHHHHHHHHHHHCCCCCHHHEEEEEEEEECCCCC
YVVPPPGALARLRSICDRYGILLIVDEVQSGVGRTGKMWAFEHEGIVPDIVASAKGLGGG
EEECCCHHHHHHHHHHHHCCEEEEEEHHHHCCCCCCCEEEEECCCCCHHHHHHCCCCCCC
LPLGAMIARSELARKWQPGSHGNTYGGNGLTCAVAYELLTMVEEELMDNAATVGAYLLEQ
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQALQQRFPQIGAVRGRGLMIGVEFVDSQGNPAGALAEAVMVESFRKGLLVLTCGASTIR
HHHHHHHCCHHCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCEE
FCPPLTLTKAQADEAVARFALAIEACLTA
CCCCCCEEHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTHTETARLPTDYMPGPVPGPRAQELVARDHTVIAPLGRVYPLVIDHAQGCEVWDVDGN
CCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEECHHHHHHHEEECCCCCEEEECCCC
RFLDMNAGIAVLAAGHCHPRLIQVAQEQLQRFTHMAGTDFYNEPMVRAAEKLVSLMPGGK
EEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
DWQVFFTNSGTEAVEASIKLARYATGRQNIIAFYGAFHGRSYGSLSLTASKPRQRQGFFP
CEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEHHCCCCCCCEEEEECCCHHHCCCCC
LLPGVSHTYYPNCYRCPINLRFPECDIACLDLIERTLFKTTTPPEEVAAIIIEPIQGEGG
CCCCCCCCCCCCEEECCEECCCCCCCHHHHHHHHHHHHCCCCCHHHEEEEEEEEECCCCC
YVVPPPGALARLRSICDRYGILLIVDEVQSGVGRTGKMWAFEHEGIVPDIVASAKGLGGG
EEECCCHHHHHHHHHHHHCCEEEEEEHHHHCCCCCCCEEEEECCCCCHHHHHHCCCCCCC
LPLGAMIARSELARKWQPGSHGNTYGGNGLTCAVAYELLTMVEEELMDNAATVGAYLLEQ
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQALQQRFPQIGAVRGRGLMIGVEFVDSQGNPAGALAEAVMVESFRKGLLVLTCGASTIR
HHHHHHHCCHHCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCEE
FCPPLTLTKAQADEAVARFALAIEACLTA
CCCCCCEEHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal 5'-phosphate lysine-residue [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 3.3 {4-aminobutanoate}} 1.1 {4-aminobutanoate}} 1.3 {2-oxoglutarate}} 0.7 {2-oxoglutarate}} [C]

Substrates: 4-aminobutanoate; 2-oxoglutarate

Specific reaction: 4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate

General reaction: Amino group transfer [C]

Inhibitor: 2-Oxoglutarate; Lysyl reagents; SH-group reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA