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The map label for this gene is bfmBAA [H]

Identifier: 222525096

GI number: 222525096

Start: 2322328

End: 2323356

Strand: Reverse

Name: bfmBAA [H]

Synonym: Chy400_1834

Alternate gene names: 222525096

Gene position: 2323356-2322328 (Counterclockwise)

Preceding gene: 222525097

Following gene: 222525095

Centisome position: 44.1

GC content: 58.89

Gene sequence:

>1029_bases
ATGACCCTCTCAATCAATGAAATTAGCACCGATCCAGGATTTGGGCACGATCTACCACCGCACACGCTACGCGATATGCT
GCGGTATATGATGCTGGCGCGAGCGCTCGACGAGCGTATGTGGGTGCTCAATCGGGCCGGCAAAGCGCCCTTTGTGATTA
GTTGTCAGGGCCACGAAGCGGCTCAGGTTGGCGCCGCGTTTGCCTTAATGCGTGGTAAAGACTTCATTCTGCCCTACTAT
CGCGGGCTGGCAACCGTCCTGGTGATGGGTATGACACCGACGGAAGTGATGCTCGGTCTGTTCGCCCGCGCCACCGATCC
CAGTTCAGGTGGACGACAGATGCCGGCCCACTACGGCTGTCGGCGCCTGAAGATTGTGACCCAATCCTCGCCGGTCGGCA
CCCAGATACCGCACGCGGCAGGGATTGGTCTGGCCGAAAAGATTAAAGGCGGTGACGCGGTAGTATGGGTATCCTTCGGT
GAGGGCACCACCAGCCAGGGTGATTTTCACGAAGGAGTCAATCTGGCAGCCGTCCACCGGCTGCCGGTTATTTTCCAATG
CGAAAATAATGAATACGCGATCAGTGTTCATCAACGCCAGCAAATGGCCATTGGCAGCGTTGCCGAACGGGCTGCGGCCT
ACGGCATTCCCGGCATTAGCGTTGATGGAACCGATGTCCTCGCCGTCTATGAAGTGACTCGCCGTGCAGTTGAACGGGCA
CGCCGTGGTGATGGCCCTACGCTGATCGAAGCACGAGTCGTGCGCATGACGGCCCACTCCAGTGACGACAACGACCGCAC
CTACCGCCCACCGCACGAGATTGCCCTTGTTCGTCATCAAGACCCAATTGTGCGCTTTGTGGCCCAGTTGCGTGAGCACG
GTATCTTGAGTGAAGCCGAAGAACAAGAGATGCGTGCCGAGGTGAAGGCAATCGTTGATCGCGCCACCGAAGAGGCCGAG
CGAGCACCGATGCCAGAACCGGAAACCCTCTACGACCACGTCTACGCGCCGTTGCGCCAGCGTCGCTGA

Upstream 100 bases:

>100_bases
AAGCGTGAGACAGCATACGCCTGCGTTGGTGCGTCCTGCTACGGCGAACCGTGGCGGGACTTTCGTTTTCAGGATATCCG
TTTAGAGAAAGGGAGCGCCG

Downstream 100 bases:

>100_bases
TATGCGGGGAGGATAAATATGCCTGAGATGAACTTGCTGGAAGCTATTCGCCAGGGCCTCGATGAAGCCATGGCTGCCGA
TTCACGGGTATTCATTTTCG

Product: 3-methyl-2-oxobutanoate dehydrogenase

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; BCKDH E1-alpha [H]

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MTLSINEISTDPGFGHDLPPHTLRDMLRYMMLARALDERMWVLNRAGKAPFVISCQGHEAAQVGAAFALMRGKDFILPYY
RGLATVLVMGMTPTEVMLGLFARATDPSSGGRQMPAHYGCRRLKIVTQSSPVGTQIPHAAGIGLAEKIKGGDAVVWVSFG
EGTTSQGDFHEGVNLAAVHRLPVIFQCENNEYAISVHQRQQMAIGSVAERAAAYGIPGISVDGTDVLAVYEVTRRAVERA
RRGDGPTLIEARVVRMTAHSSDDNDRTYRPPHEIALVRHQDPIVRFVAQLREHGILSEAEEQEMRAEVKAIVDRATEEAE
RAPMPEPETLYDHVYAPLRQRR

Sequences:

>Translated_342_residues
MTLSINEISTDPGFGHDLPPHTLRDMLRYMMLARALDERMWVLNRAGKAPFVISCQGHEAAQVGAAFALMRGKDFILPYY
RGLATVLVMGMTPTEVMLGLFARATDPSSGGRQMPAHYGCRRLKIVTQSSPVGTQIPHAAGIGLAEKIKGGDAVVWVSFG
EGTTSQGDFHEGVNLAAVHRLPVIFQCENNEYAISVHQRQQMAIGSVAERAAAYGIPGISVDGTDVLAVYEVTRRAVERA
RRGDGPTLIEARVVRMTAHSSDDNDRTYRPPHEIALVRHQDPIVRFVAQLREHGILSEAEEQEMRAEVKAIVDRATEEAE
RAPMPEPETLYDHVYAPLRQRR
>Mature_341_residues
TLSINEISTDPGFGHDLPPHTLRDMLRYMMLARALDERMWVLNRAGKAPFVISCQGHEAAQVGAAFALMRGKDFILPYYR
GLATVLVMGMTPTEVMLGLFARATDPSSGGRQMPAHYGCRRLKIVTQSSPVGTQIPHAAGIGLAEKIKGGDAVVWVSFGE
GTTSQGDFHEGVNLAAVHRLPVIFQCENNEYAISVHQRQQMAIGSVAERAAAYGIPGISVDGTDVLAVYEVTRRAVERAR
RGDGPTLIEARVVRMTAHSSDDNDRTYRPPHEIALVRHQDPIVRFVAQLREHGILSEAEEQEMRAEVKAIVDRATEEAER
APMPEPETLYDHVYAPLRQRR

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCKDHA family [H]

Homologues:

Organism=Homo sapiens, GI11386135, Length=328, Percent_Identity=38.109756097561, Blast_Score=199, Evalue=4e-51,
Organism=Homo sapiens, GI258645172, Length=328, Percent_Identity=38.109756097561, Blast_Score=194, Evalue=7e-50,
Organism=Homo sapiens, GI4885543, Length=296, Percent_Identity=33.1081081081081, Blast_Score=138, Evalue=8e-33,
Organism=Homo sapiens, GI291084742, Length=295, Percent_Identity=33.2203389830508, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI4505685, Length=295, Percent_Identity=33.2203389830508, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI291084744, Length=214, Percent_Identity=37.8504672897196, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI291084757, Length=201, Percent_Identity=34.8258706467662, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI86563357, Length=338, Percent_Identity=35.7988165680473, Blast_Score=183, Evalue=9e-47,
Organism=Caenorhabditis elegans, GI86563355, Length=338, Percent_Identity=35.7988165680473, Blast_Score=183, Evalue=9e-47,
Organism=Caenorhabditis elegans, GI17536047, Length=289, Percent_Identity=32.8719723183391, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI32564172, Length=289, Percent_Identity=32.8719723183391, Blast_Score=128, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6321026, Length=333, Percent_Identity=31.2312312312312, Blast_Score=142, Evalue=9e-35,
Organism=Drosophila melanogaster, GI21355903, Length=337, Percent_Identity=32.9376854599407, Blast_Score=175, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24639746, Length=287, Percent_Identity=33.1010452961672, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24639748, Length=287, Percent_Identity=33.4494773519164, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24639740, Length=286, Percent_Identity=33.2167832167832, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24639744, Length=286, Percent_Identity=33.2167832167832, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI28571106, Length=286, Percent_Identity=33.2167832167832, Blast_Score=129, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 37772; Mature: 37640

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSINEISTDPGFGHDLPPHTLRDMLRYMMLARALDERMWVLNRAGKAPFVISCQGHEA
CEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCH
AQVGAAFALMRGKDFILPYYRGLATVLVMGMTPTEVMLGLFARATDPSSGGRQMPAHYGC
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHCC
RRLKIVTQSSPVGTQIPHAAGIGLAEKIKGGDAVVWVSFGEGTTSQGDFHEGVNLAAVHR
EEEEEEECCCCCCCCCCCHHCCCHHHHCCCCCEEEEEEECCCCCCCCCHHCCCCEEEEEC
LPVIFQCENNEYAISVHQRQQMAIGSVAERAAAYGIPGISVDGTDVLAVYEVTRRAVERA
CCEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
RRGDGPTLIEARVVRMTAHSSDDNDRTYRPPHEIALVRHQDPIVRFVAQLREHGILSEAE
HCCCCCCEEHHEEEEEEECCCCCCCCCCCCHHHEEEEECCCHHHHHHHHHHHCCCCCHHH
EQEMRAEVKAIVDRATEEAERAPMPEPETLYDHVYAPLRQRR
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLSINEISTDPGFGHDLPPHTLRDMLRYMMLARALDERMWVLNRAGKAPFVISCQGHEA
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCH
AQVGAAFALMRGKDFILPYYRGLATVLVMGMTPTEVMLGLFARATDPSSGGRQMPAHYGC
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHCC
RRLKIVTQSSPVGTQIPHAAGIGLAEKIKGGDAVVWVSFGEGTTSQGDFHEGVNLAAVHR
EEEEEEECCCCCCCCCCCHHCCCHHHHCCCCCEEEEEEECCCCCCCCCHHCCCCEEEEEC
LPVIFQCENNEYAISVHQRQQMAIGSVAERAAAYGIPGISVDGTDVLAVYEVTRRAVERA
CCEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
RRGDGPTLIEARVVRMTAHSSDDNDRTYRPPHEIALVRHQDPIVRFVAQLREHGILSEAE
HCCCCCCEEHHEEEEEEECCCCCCCCCCCCHHHEEEEECCCHHHHHHHHHHHCCCCCHHH
EQEMRAEVKAIVDRATEEAERAPMPEPETLYDHVYAPLRQRR
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377 [H]