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The map label for this gene is rpfG [H]

Identifier: 222525081

GI number: 222525081

Start: 2297099

End: 2298580

Strand: Direct

Name: rpfG [H]

Synonym: Chy400_1818

Alternate gene names: 222525081

Gene position: 2297099-2298580 (Clockwise)

Preceding gene: 222525080

Following gene: 222525082

Centisome position: 43.6

GC content: 53.98

Gene sequence:

>1482_bases
ATGGCGGACTACGCAAACTCTTCCCCTGAGCGCCCCCGGATTCTCATTGTGGAGGACGAAGTCAATATCCGTGAATTCTG
TCGTCTGCTCCTCCGTCGGCATTACGATGTCGTTGCGGTTGGGGATGGTCTCGCAGCGATAGAGTTGCTGTCTACGCAGA
CGTTTGATCTGGTGTTGACCGATCTGCAAATGCCGAATCTGGATGGGATTGCGCTTGTTCAATACTTGCGTGCCCATCAT
CTGGAAACCGATGCGGTCGTTATGACGGCACATGCGACGGTCGAAACTGCACGCGAAGCACTTAAGCTCGGTGCTCTTGA
CTACATTGCCAAACCTGTTGATGCCGAGCAATTACAAAAGACAGTACGAACGGCACTGGAATTGCGTCGTGTGCGCCGCG
AGAAGGAGCGTCTCTCCGACCTGGTGCTAATGTATCAGTTTAGCCAGGTGATTACTGCTTCGCTCGATGTTCAGGAACAA
TCCGCTCACCTACTTGAATTCGTCGGTCGTCGTTTTGCCCCAAGCAACATCGGATTGTCGCTAATTGACGCTGATGGTGA
TACGCTGGTGGTGCTGATCGGTCCCGAACCCGGCCAGAGTCTGTCCCTGAGCGAACGTGACGAACAGAGTCTGCGAGCGG
CGCACTACCAGTTGATCGAATGTCCGACCACGTCATCAGATCGCCTCATCGAAGTAGTCTTGCGGAGTCGCGACCATGCC
ATTGGCTTCCTCAATTTGACCCGCAATAGTGAACAACCCTCGTTCGATGCCGCTGATCGGCATCTGATCGAAATTTTTGC
TTCGCAGATTGCCGCTGCGCTGGACAATGCGCGTCTGTATCGTGCCCTCAAAGATCAATACGAGCAGATGATTGCTGCGC
TGGCCGGGGCAATCGAAGCCCGTGATGCCTATACCTATGGCCATTCGCGCCAGGTTAGTCGCTACGCTGTCCGCCTGGCG
CAAGAATTGGGTCTCAGTCCTGCGGAGATTGAGCGCATTCACTATGCCGGTCTCTTGCATGATATTGGTAAGATTGGTAT
TCGCGACGATATCTTACTCAAACCGGGTCCTCTTTCACCTGAAGAGCTGACCCAGATGCGCCGCCATCCGCAAATCGGAG
TACGTATTCTTGAGCAGATCAGTGGCTTACGTGATATATTGCCGATTATCGCTGCCCACCACGAACGTTTCGATGGTACC
GGCTATCCATACGGTTTGAAGGGAGAAGAGATACCATTCGAGGCGCGTATTCTAGCGGTGGCCGATACCTTCGAGGCGCT
AACCGCTGACCGCGCCTACCGTTCGGCGATGAGTCCTGAAGCGGTGCTCCAGATCATCCAGGATGGTAGGGGTACCCATT
GGGATCCGCAGGTGGTTGATGCTTTTTTGCGACTGATGCGCCGTGAGCGGTTATGGGAACAATCACCACGGTTGCGGCAG
GTGATACAACCACCATTGTCGGTAGAGTCAATTTCTTCGTGA

Upstream 100 bases:

>100_bases
CGGTCATTGTAGCCTTTATGCAAACTTAGCTTGCTACATTACAAACGAAATAGCCGGCAATCTGCTCTGTGCAGATGTGC
ATGTCTGGGGAGCCTGATCT

Downstream 100 bases:

>100_bases
CATAAGCTCATTCCGCGTTAGCGACCATCTACGATGCAATCAGCCGCTGGATTGGTTGCCTTGCGCGCTCGTCATGCCCG
TAGCGTATCATTTGGCGTGC

Product: response regulator receiver modulated metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MADYANSSPERPRILIVEDEVNIREFCRLLLRRHYDVVAVGDGLAAIELLSTQTFDLVLTDLQMPNLDGIALVQYLRAHH
LETDAVVMTAHATVETAREALKLGALDYIAKPVDAEQLQKTVRTALELRRVRREKERLSDLVLMYQFSQVITASLDVQEQ
SAHLLEFVGRRFAPSNIGLSLIDADGDTLVVLIGPEPGQSLSLSERDEQSLRAAHYQLIECPTTSSDRLIEVVLRSRDHA
IGFLNLTRNSEQPSFDAADRHLIEIFASQIAAALDNARLYRALKDQYEQMIAALAGAIEARDAYTYGHSRQVSRYAVRLA
QELGLSPAEIERIHYAGLLHDIGKIGIRDDILLKPGPLSPEELTQMRRHPQIGVRILEQISGLRDILPIIAAHHERFDGT
GYPYGLKGEEIPFEARILAVADTFEALTADRAYRSAMSPEAVLQIIQDGRGTHWDPQVVDAFLRLMRRERLWEQSPRLRQ
VIQPPLSVESISS

Sequences:

>Translated_493_residues
MADYANSSPERPRILIVEDEVNIREFCRLLLRRHYDVVAVGDGLAAIELLSTQTFDLVLTDLQMPNLDGIALVQYLRAHH
LETDAVVMTAHATVETAREALKLGALDYIAKPVDAEQLQKTVRTALELRRVRREKERLSDLVLMYQFSQVITASLDVQEQ
SAHLLEFVGRRFAPSNIGLSLIDADGDTLVVLIGPEPGQSLSLSERDEQSLRAAHYQLIECPTTSSDRLIEVVLRSRDHA
IGFLNLTRNSEQPSFDAADRHLIEIFASQIAAALDNARLYRALKDQYEQMIAALAGAIEARDAYTYGHSRQVSRYAVRLA
QELGLSPAEIERIHYAGLLHDIGKIGIRDDILLKPGPLSPEELTQMRRHPQIGVRILEQISGLRDILPIIAAHHERFDGT
GYPYGLKGEEIPFEARILAVADTFEALTADRAYRSAMSPEAVLQIIQDGRGTHWDPQVVDAFLRLMRRERLWEQSPRLRQ
VIQPPLSVESISS
>Mature_492_residues
ADYANSSPERPRILIVEDEVNIREFCRLLLRRHYDVVAVGDGLAAIELLSTQTFDLVLTDLQMPNLDGIALVQYLRAHHL
ETDAVVMTAHATVETAREALKLGALDYIAKPVDAEQLQKTVRTALELRRVRREKERLSDLVLMYQFSQVITASLDVQEQS
AHLLEFVGRRFAPSNIGLSLIDADGDTLVVLIGPEPGQSLSLSERDEQSLRAAHYQLIECPTTSSDRLIEVVLRSRDHAI
GFLNLTRNSEQPSFDAADRHLIEIFASQIAAALDNARLYRALKDQYEQMIAALAGAIEARDAYTYGHSRQVSRYAVRLAQ
ELGLSPAEIERIHYAGLLHDIGKIGIRDDILLKPGPLSPEELTQMRRHPQIGVRILEQISGLRDILPIIAAHHERFDGTG
YPYGLKGEEIPFEARILAVADTFEALTADRAYRSAMSPEAVLQIIQDGRGTHWDPQVVDAFLRLMRRERLWEQSPRLRQV
IQPPLSVESISS

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG2206

COG function: function code T; HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=157, Percent_Identity=35.031847133758, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790437, Length=114, Percent_Identity=42.9824561403509, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1790299, Length=118, Percent_Identity=30.5084745762712, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI145693157, Length=136, Percent_Identity=31.6176470588235, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786784, Length=115, Percent_Identity=34.7826086956522, Blast_Score=62, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6322000, Length=106, Percent_Identity=35.8490566037736, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 55361; Mature: 55230

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADYANSSPERPRILIVEDEVNIREFCRLLLRRHYDVVAVGDGLAAIELLSTQTFDLVLT
CCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCHHHHHEE
DLQMPNLDGIALVQYLRAHHLETDAVVMTAHATVETAREALKLGALDYIAKPVDAEQLQK
CCCCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
TVRTALELRRVRREKERLSDLVLMYQFSQVITASLDVQEQSAHLLEFVGRRFAPSNIGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEE
LIDADGDTLVVLIGPEPGQSLSLSERDEQSLRAAHYQLIECPTTSSDRLIEVVLRSRDHA
EEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCCCE
IGFLNLTRNSEQPSFDAADRHLIEIFASQIAAALDNARLYRALKDQYEQMIAALAGAIEA
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDAYTYGHSRQVSRYAVRLAQELGLSPAEIERIHYAGLLHDIGKIGIRDDILLKPGPLSP
HHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCH
EELTQMRRHPQIGVRILEQISGLRDILPIIAAHHERFDGTGYPYGLKGEEIPFEARILAV
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEH
ADTFEALTADRAYRSAMSPEAVLQIIQDGRGTHWDPQVVDAFLRLMRRERLWEQSPRLRQ
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
VIQPPLSVESISS
HHCCCCCHHCCCC
>Mature Secondary Structure 
ADYANSSPERPRILIVEDEVNIREFCRLLLRRHYDVVAVGDGLAAIELLSTQTFDLVLT
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCHHHHHEE
DLQMPNLDGIALVQYLRAHHLETDAVVMTAHATVETAREALKLGALDYIAKPVDAEQLQK
CCCCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
TVRTALELRRVRREKERLSDLVLMYQFSQVITASLDVQEQSAHLLEFVGRRFAPSNIGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEE
LIDADGDTLVVLIGPEPGQSLSLSERDEQSLRAAHYQLIECPTTSSDRLIEVVLRSRDHA
EEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCCCE
IGFLNLTRNSEQPSFDAADRHLIEIFASQIAAALDNARLYRALKDQYEQMIAALAGAIEA
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDAYTYGHSRQVSRYAVRLAQELGLSPAEIERIHYAGLLHDIGKIGIRDDILLKPGPLSP
HHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCH
EELTQMRRHPQIGVRILEQISGLRDILPIIAAHHERFDGTGYPYGLKGEEIPFEARILAV
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEH
ADTFEALTADRAYRSAMSPEAVLQIIQDGRGTHWDPQVVDAFLRLMRRERLWEQSPRLRQ
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
VIQPPLSVESISS
HHCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]