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The map label for this gene is purF [H]

Identifier: 222525003

GI number: 222525003

Start: 2192653

End: 2194125

Strand: Reverse

Name: purF [H]

Synonym: Chy400_1738

Alternate gene names: 222525003

Gene position: 2194125-2192653 (Counterclockwise)

Preceding gene: 222525007

Following gene: 222525000

Centisome position: 41.64

GC content: 56.82

Gene sequence:

>1473_bases
ATGAACGATCAATCGCTGCCCGGCGTACCTGATCGTAATGATACCATTGCCGCTCAATGTATGCTTCCCACTGTATCTGA
TGACAAGCCGGGTCATGAGTGCGGTGTTTTCGGCATTGTCGCTGCCGATGCTGACGTTGCGCGCCTCACCTTTTTCGGTC
TCTACGCGCTTCAGCATCGTGGTCAAGAGAGTGCCGGTATCGCGGTGAGCAATGGGCGGAACATTCGTTACTACAAAAAC
ATGGGTTTAGTGGCGCAAGTGTTCGATGAAGAGAAGTTGCGCCCGCTCAGCGGTTATATGGCGATTGGGCATACGCGCTA
TTCAACCACCGGTTCATCAAAGCTGGAAAACGCCCAGCCGTTCGTTGTCGAGAGTGCATTAGGGCCACTGGCGGTTGGTC
ACAACGGTAATCTGACTAATGCTGCCGCATTACGACGTGAGTTGTTGCAACGCGGTGTCGGTCTGACCAGCAGCAGCGAT
AGTGAAGTCATTACGCAAATGCTGGCCGGTGGCGAAGGGCGTACCTGGGAAGAGAAGCTCAAGGTCTTCATGGTACGGGC
ACAGGGTGCGTACTGTCTTACCGTACTGACCCGCGATGCACTCTACGCGGTGCGCGATCCGTGGGGATTACACCCGCTCT
GCCTGGGTCAGCTCAGTGACAATGGTTGGGTGGTGGCTTCGGAAAGCTGTGCTTTGGGGACGATTGGCGCTGAATTCGTG
CGTGAGATCGAGCCGGGTGAAATTGTCAAAATTACGCTTGACGGCCCCCAGACCATCTCGCACCAACCGTCGCTGCGCAC
GGCAGCCTGTCTGTTTGAGTACATCTATTTCGCGCGTCCCGACAGCATGCTGCATGGAACGGTTCTCCACGCCATCCGGG
TCGCGCAGGGGCGAGAACTGGCCCGCGAAGCGCCGTGCGATGCCGATATTGTGATTGCGGTTCCCGACAGTGCAACGCCG
GCAGCAATCGGTTATGCACAAGAGTCGGGTATCCCCTACTCGGAAGGGTTGATTAAGAACCGCTACATCGGACGAACGTT
CATTCAGCCTGATGATCGATTGCGTCAGCTTGGTATTGCGCTCAAATTCAACGCCCTGACCGATAATCTGGCCGGCAAAC
GGGTGGTGTTGGTTGATGATAGTATCGTGCGGGGCAACACCAGTGGGCCAATCGTGCGTCTGCTGCGTGAGGCCGGTGCG
CGCGAGGTTCACGTGCGCGTCTCGTCACCACCCATTCGCCACCCCTGCTTCCTTGGCGTCGATATGGCAACCTATCCTGA
GCTGATTGCCCATCGCCTGACCATCGAAGGCATTCGCCAGCATCTCGGTGCCGACAGCCTGGCCTACCTGAGCCTCGAAG
GCTTGATTCGCTCAACCGGACGTGATCCTTCTACTTTTTGCACCGGCTGCTTCACCGGTCAATACCCGGTTGAGGTTGAG
CTGGTTGACAAAGAGGTGTTTGAGGTGCGCTGA

Upstream 100 bases:

>100_bases
TCTGTCGTTAGGTTGTCTGAAGGTTTCATAATCCGTGATATACTTTAATCAGCAATGATGCGAACTGTCCCCAAATCTGC
GCGCAGGAGGATAACTTCTC

Downstream 100 bases:

>100_bases
AGATAGATAGGCCCCGCCGACGCATATGTGGGGGAGTAGGGAGTGGGGAATTGGCATGTTCCGTATGGCGCCTGGTCACC
TTGTTTCCGCCCCCCTCCCG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MNDQSLPGVPDRNDTIAAQCMLPTVSDDKPGHECGVFGIVAADADVARLTFFGLYALQHRGQESAGIAVSNGRNIRYYKN
MGLVAQVFDEEKLRPLSGYMAIGHTRYSTTGSSKLENAQPFVVESALGPLAVGHNGNLTNAAALRRELLQRGVGLTSSSD
SEVITQMLAGGEGRTWEEKLKVFMVRAQGAYCLTVLTRDALYAVRDPWGLHPLCLGQLSDNGWVVASESCALGTIGAEFV
REIEPGEIVKITLDGPQTISHQPSLRTAACLFEYIYFARPDSMLHGTVLHAIRVAQGRELAREAPCDADIVIAVPDSATP
AAIGYAQESGIPYSEGLIKNRYIGRTFIQPDDRLRQLGIALKFNALTDNLAGKRVVLVDDSIVRGNTSGPIVRLLREAGA
REVHVRVSSPPIRHPCFLGVDMATYPELIAHRLTIEGIRQHLGADSLAYLSLEGLIRSTGRDPSTFCTGCFTGQYPVEVE
LVDKEVFEVR

Sequences:

>Translated_490_residues
MNDQSLPGVPDRNDTIAAQCMLPTVSDDKPGHECGVFGIVAADADVARLTFFGLYALQHRGQESAGIAVSNGRNIRYYKN
MGLVAQVFDEEKLRPLSGYMAIGHTRYSTTGSSKLENAQPFVVESALGPLAVGHNGNLTNAAALRRELLQRGVGLTSSSD
SEVITQMLAGGEGRTWEEKLKVFMVRAQGAYCLTVLTRDALYAVRDPWGLHPLCLGQLSDNGWVVASESCALGTIGAEFV
REIEPGEIVKITLDGPQTISHQPSLRTAACLFEYIYFARPDSMLHGTVLHAIRVAQGRELAREAPCDADIVIAVPDSATP
AAIGYAQESGIPYSEGLIKNRYIGRTFIQPDDRLRQLGIALKFNALTDNLAGKRVVLVDDSIVRGNTSGPIVRLLREAGA
REVHVRVSSPPIRHPCFLGVDMATYPELIAHRLTIEGIRQHLGADSLAYLSLEGLIRSTGRDPSTFCTGCFTGQYPVEVE
LVDKEVFEVR
>Mature_490_residues
MNDQSLPGVPDRNDTIAAQCMLPTVSDDKPGHECGVFGIVAADADVARLTFFGLYALQHRGQESAGIAVSNGRNIRYYKN
MGLVAQVFDEEKLRPLSGYMAIGHTRYSTTGSSKLENAQPFVVESALGPLAVGHNGNLTNAAALRRELLQRGVGLTSSSD
SEVITQMLAGGEGRTWEEKLKVFMVRAQGAYCLTVLTRDALYAVRDPWGLHPLCLGQLSDNGWVVASESCALGTIGAEFV
REIEPGEIVKITLDGPQTISHQPSLRTAACLFEYIYFARPDSMLHGTVLHAIRVAQGRELAREAPCDADIVIAVPDSATP
AAIGYAQESGIPYSEGLIKNRYIGRTFIQPDDRLRQLGIALKFNALTDNLAGKRVVLVDDSIVRGNTSGPIVRLLREAGA
REVHVRVSSPPIRHPCFLGVDMATYPELIAHRLTIEGIRQHLGADSLAYLSLEGLIRSTGRDPSTFCTGCFTGQYPVEVE
LVDKEVFEVR

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=506, Percent_Identity=49.2094861660079, Blast_Score=474, Evalue=1e-134,
Organism=Escherichia coli, GI1788651, Length=468, Percent_Identity=40.3846153846154, Blast_Score=301, Evalue=6e-83,
Organism=Escherichia coli, GI1790167, Length=231, Percent_Identity=32.9004329004329, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17554892, Length=480, Percent_Identity=44.1666666666667, Blast_Score=381, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6323958, Length=481, Percent_Identity=37.6299376299376, Blast_Score=273, Evalue=5e-74,
Organism=Drosophila melanogaster, GI24659598, Length=482, Percent_Identity=50.207468879668, Blast_Score=485, Evalue=1e-137,
Organism=Drosophila melanogaster, GI28573187, Length=486, Percent_Identity=49.7942386831276, Blast_Score=479, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24659604, Length=461, Percent_Identity=50.7592190889371, Blast_Score=472, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 53189; Mature: 53189

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDQSLPGVPDRNDTIAAQCMLPTVSDDKPGHECGVFGIVAADADVARLTFFGLYALQHR
CCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHC
GQESAGIAVSNGRNIRYYKNMGLVAQVFDEEKLRPLSGYMAIGHTRYSTTGSSKLENAQP
CCCCCCEEEECCCEEEEEECCCHHEEHHCHHHCCCCCCEEEECCEEECCCCCCCCCCCCC
FVVESALGPLAVGHNGNLTNAAALRRELLQRGVGLTSSSDSEVITQMLAGGEGRTWEEKL
EEEECCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHE
KVFMVRAQGAYCLTVLTRDALYAVRDPWGLHPLCLGQLSDNGWVVASESCALGTIGAEFV
EEEEEECCCCEEEEEEHHHHHHEECCCCCCCCEEEEEECCCCEEEEECCCCEECHHHHHH
REIEPGEIVKITLDGPQTISHQPSLRTAACLFEYIYFARPDSMLHGTVLHAIRVAQGREL
HHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHH
AREAPCDADIVIAVPDSATPAAIGYAQESGIPYSEGLIKNRYIGRTFIQPDDRLRQLGIA
HHHCCCCCEEEEECCCCCCCCEEEEHHHCCCCHHHCCHHCCCCCCEECCCHHHHHHCCEE
LKFNALTDNLAGKRVVLVDDSIVRGNTSGPIVRLLREAGAREVHVRVSSPPIRHPCFLGV
EEEECHHCCCCCCEEEEECCCEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCCCEEECC
DMATYPELIAHRLTIEGIRQHLGADSLAYLSLEGLIRSTGRDPSTFCTGCFTGQYPVEVE
CHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCEEEEEECCCCCEEEE
LVDKEVFEVR
EECCHHHHCC
>Mature Secondary Structure
MNDQSLPGVPDRNDTIAAQCMLPTVSDDKPGHECGVFGIVAADADVARLTFFGLYALQHR
CCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHC
GQESAGIAVSNGRNIRYYKNMGLVAQVFDEEKLRPLSGYMAIGHTRYSTTGSSKLENAQP
CCCCCCEEEECCCEEEEEECCCHHEEHHCHHHCCCCCCEEEECCEEECCCCCCCCCCCCC
FVVESALGPLAVGHNGNLTNAAALRRELLQRGVGLTSSSDSEVITQMLAGGEGRTWEEKL
EEEECCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHE
KVFMVRAQGAYCLTVLTRDALYAVRDPWGLHPLCLGQLSDNGWVVASESCALGTIGAEFV
EEEEEECCCCEEEEEEHHHHHHEECCCCCCCCEEEEEECCCCEEEEECCCCEECHHHHHH
REIEPGEIVKITLDGPQTISHQPSLRTAACLFEYIYFARPDSMLHGTVLHAIRVAQGREL
HHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCHHH
AREAPCDADIVIAVPDSATPAAIGYAQESGIPYSEGLIKNRYIGRTFIQPDDRLRQLGIA
HHHCCCCCEEEEECCCCCCCCEEEEHHHCCCCHHHCCHHCCCCCCEECCCHHHHHHCCEE
LKFNALTDNLAGKRVVLVDDSIVRGNTSGPIVRLLREAGAREVHVRVSSPPIRHPCFLGV
EEEECHHCCCCCCEEEEECCCEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCCCEEECC
DMATYPELIAHRLTIEGIRQHLGADSLAYLSLEGLIRSTGRDPSTFCTGCFTGQYPVEVE
CHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCEEEEEECCCCCEEEE
LVDKEVFEVR
EECCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8809759 [H]