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The map label for this gene is gatA [H]

Identifier: 222525002

GI number: 222525002

Start: 2190660

End: 2192219

Strand: Direct

Name: gatA [H]

Synonym: Chy400_1737

Alternate gene names: 222525002

Gene position: 2190660-2192219 (Clockwise)

Preceding gene: 222525001

Following gene: 222525004

Centisome position: 41.58

GC content: 61.73

Gene sequence:

>1560_bases
ATGACGTTCAACGATCCCACCAGTTCCATCGGAACGCTGGCTTACGAAGCGACCATTGCCGATCTTCAGGCGGCAATGGC
AGCCGGTACCCTTACCGCAGAAGCGCTCACAATGGCCTGCCTTGAGCGGATCAATGCGCTGAATCGGGCCGGGCCGTGCC
TGAATGCGCTGATCGAAGTCAGCCCAAGCGCCCTCGAAACCGCAATTGCCCTCGACGCTGAACGTGACGTGCGCGGCCCG
CGCAGCCCGCTCCACGGCATTCCAATCGTGCTCAAGGACAACATCGATACGCTCGACGACACTGCCACGACTGCCGGTTC
CCTGGCCCTGATCGGCTCGCGACCGGCTGCTGAAGCAACGGTTGCCGCCCGTCTACGAGCTGCCGGTGCCGTGCTGTTGG
GTAAAGCTAACTTGAGCGAATGGGCGAATTTCCGCAGTACCAGCTCATCAAGTGGCTGGAGTGCGCGGGGTGGTCAGGCG
CGCAACCCCTATGTGCTGAGCCGGAGTCCGTGTGGCTCCAGTTCGGGGTCGGCCATCGCAGTGGCAGCCAGTATGTGTGT
AGCGGCGATTGGTACCGAAACCGATGGTTCTATTTCGTGCCCATCGGCGATGTGTGGTGTGGTGGGAATTAAACCGACGG
TTGGCCTGACCAGCCGCGCCGGTGTGATCCCAATCAGCTCCACCCAGGACACCGTCGGCCCGCACGCCCGTTGTGTAGCC
GACGCCGCCACGGTGCTGGGGATCATTGCCGGCCCTGACCCGCACGATCCGGCGACCACAGCCGCCGCCGGACACGTTCG
CCCGGATTACCGCACCTGTCTTCAGGCCGATGCACTGCGTGGCGCACGGATCGGGGTGTTGCGCAGTGATCGCTTCGCCG
GCTTTGGCCGCCACGTTGAACAGGCATTTGCGGCAGCACTGACAGCAATGCGCGATGCCGGTGCTCATGTGGTTGATCCT
GTGACATTTCCTGATGAGTTGCTGGCATTTAATGAAGCCGAGTTAACGGTTCTGCTGTACGAATTCAAAGCCACGCTCAA
CCGCTACCTTGCCAGCCGCGTGCCTGATCCGCAGGCAGCCACCCCTGCGCCACGTTCGTTAGAGGAATTGATCGCCTTTA
ACGAACAACAGGCCGAACACGAGTTACGCTTCTTCGGTCAGGAACTGCTTGTCCAGGCCGCCGCCGTTGGCGACCTCGAT
GATCCGGCGTATCAACAGGCCCTGGCTGCCAGTCGTGATGCTACTCGTCAGGCGCTCGACGCCCTGCTCTACGAACAGCA
GCTCGATGCCCTGGTCGCGCCGGCAACCGGTCTGGCCTGGCCGATTGATTTGATCGGCGGCGATCGCTACCCTGGCGGGA
GCAGTTCGCTCGCGGCGCGAGCCGGCTATCCAATGGTGACGGTACCGGCAGGGATGGCGTTCGGTTTACCAATCGCGATC
AACTTCATCGGCACCGCATGGTCTGAACCGATGCTGATCCGATTGGCTTACGCTTTCGAGCAGGCAACGAAACTCCGGCG
ACCACCGGTGTATCGTCAATGGATTGTTGGCGATGAGTAG

Upstream 100 bases:

>100_bases
AGGTACTGTAGCGCCTGACAGGCTATCCCTGCCCAACCCACGTGAGCCGGGCAGGGATAGTTAATATTATCTGCCGGTGA
TGCCTGGAAATGAGGATGCT

Downstream 100 bases:

>100_bases
GGGGTAGGGGGGAGGAGATAGTAGATAGGAGATAGGAGATAGAAGATAGGAGATAGAAGATAGGAGATAGAAGATAGGAG
ATAGAAGATAGGAGATAGAA

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MTFNDPTSSIGTLAYEATIADLQAAMAAGTLTAEALTMACLERINALNRAGPCLNALIEVSPSALETAIALDAERDVRGP
RSPLHGIPIVLKDNIDTLDDTATTAGSLALIGSRPAAEATVAARLRAAGAVLLGKANLSEWANFRSTSSSSGWSARGGQA
RNPYVLSRSPCGSSSGSAIAVAASMCVAAIGTETDGSISCPSAMCGVVGIKPTVGLTSRAGVIPISSTQDTVGPHARCVA
DAATVLGIIAGPDPHDPATTAAAGHVRPDYRTCLQADALRGARIGVLRSDRFAGFGRHVEQAFAAALTAMRDAGAHVVDP
VTFPDELLAFNEAELTVLLYEFKATLNRYLASRVPDPQAATPAPRSLEELIAFNEQQAEHELRFFGQELLVQAAAVGDLD
DPAYQQALAASRDATRQALDALLYEQQLDALVAPATGLAWPIDLIGGDRYPGGSSSLAARAGYPMVTVPAGMAFGLPIAI
NFIGTAWSEPMLIRLAYAFEQATKLRRPPVYRQWIVGDE

Sequences:

>Translated_519_residues
MTFNDPTSSIGTLAYEATIADLQAAMAAGTLTAEALTMACLERINALNRAGPCLNALIEVSPSALETAIALDAERDVRGP
RSPLHGIPIVLKDNIDTLDDTATTAGSLALIGSRPAAEATVAARLRAAGAVLLGKANLSEWANFRSTSSSSGWSARGGQA
RNPYVLSRSPCGSSSGSAIAVAASMCVAAIGTETDGSISCPSAMCGVVGIKPTVGLTSRAGVIPISSTQDTVGPHARCVA
DAATVLGIIAGPDPHDPATTAAAGHVRPDYRTCLQADALRGARIGVLRSDRFAGFGRHVEQAFAAALTAMRDAGAHVVDP
VTFPDELLAFNEAELTVLLYEFKATLNRYLASRVPDPQAATPAPRSLEELIAFNEQQAEHELRFFGQELLVQAAAVGDLD
DPAYQQALAASRDATRQALDALLYEQQLDALVAPATGLAWPIDLIGGDRYPGGSSSLAARAGYPMVTVPAGMAFGLPIAI
NFIGTAWSEPMLIRLAYAFEQATKLRRPPVYRQWIVGDE
>Mature_518_residues
TFNDPTSSIGTLAYEATIADLQAAMAAGTLTAEALTMACLERINALNRAGPCLNALIEVSPSALETAIALDAERDVRGPR
SPLHGIPIVLKDNIDTLDDTATTAGSLALIGSRPAAEATVAARLRAAGAVLLGKANLSEWANFRSTSSSSGWSARGGQAR
NPYVLSRSPCGSSSGSAIAVAASMCVAAIGTETDGSISCPSAMCGVVGIKPTVGLTSRAGVIPISSTQDTVGPHARCVAD
AATVLGIIAGPDPHDPATTAAAGHVRPDYRTCLQADALRGARIGVLRSDRFAGFGRHVEQAFAAALTAMRDAGAHVVDPV
TFPDELLAFNEAELTVLLYEFKATLNRYLASRVPDPQAATPAPRSLEELIAFNEQQAEHELRFFGQELLVQAAAVGDLDD
PAYQQALAASRDATRQALDALLYEQQLDALVAPATGLAWPIDLIGGDRYPGGSSSLAARAGYPMVTVPAGMAFGLPIAIN
FIGTAWSEPMLIRLAYAFEQATKLRRPPVYRQWIVGDE

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=545, Percent_Identity=27.8899082568807, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI195972892, Length=221, Percent_Identity=28.5067873303167, Blast_Score=82, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556264, Length=300, Percent_Identity=26, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17543272, Length=184, Percent_Identity=33.1521739130435, Blast_Score=80, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17537465, Length=255, Percent_Identity=27.843137254902, Blast_Score=78, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6319685, Length=183, Percent_Identity=34.4262295081967, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323950, Length=294, Percent_Identity=26.8707482993197, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648113, Length=519, Percent_Identity=30.4431599229287, Blast_Score=135, Evalue=8e-32,
Organism=Drosophila melanogaster, GI24644968, Length=258, Percent_Identity=29.4573643410853, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI161078093, Length=512, Percent_Identity=23.828125, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356731, Length=233, Percent_Identity=30.0429184549356, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648435, Length=262, Percent_Identity=25.5725190839695, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648437, Length=262, Percent_Identity=25.5725190839695, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45550774, Length=258, Percent_Identity=25.5813953488372, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 54146; Mature: 54015

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFNDPTSSIGTLAYEATIADLQAAMAAGTLTAEALTMACLERINALNRAGPCLNALIEV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
SPSALETAIALDAERDVRGPRSPLHGIPIVLKDNIDTLDDTATTAGSLALIGSRPAAEAT
CCHHHHHHHHCCCHHCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCHHHH
VAARLRAAGAVLLGKANLSEWANFRSTSSSSGWSARGGQARNPYVLSRSPCGSSSGSAIA
HHHHHHHCCEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHH
VAASMCVAAIGTETDGSISCPSAMCGVVGIKPTVGLTSRAGVIPISSTQDTVGPHARCVA
HHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHH
DAATVLGIIAGPDPHDPATTAAAGHVRPDYRTCLQADALRGARIGVLRSDRFAGFGRHVE
HHHHHHHEECCCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHH
QAFAAALTAMRDAGAHVVDPVTFPDELLAFNEAELTVLLYEFKATLNRYLASRVPDPQAA
HHHHHHHHHHHHCCCCEECCCCCCHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCC
TPAPRSLEELIAFNEQQAEHELRFFGQELLVQAAAVGDLDDPAYQQALAASRDATRQALD
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
ALLYEQQLDALVAPATGLAWPIDLIGGDRYPGGSSSLAARAGYPMVTVPAGMAFGLPIAI
HHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCHHHCCCCEEEECCCCHHCCCEEE
NFIGTAWSEPMLIRLAYAFEQATKLRRPPVYRQWIVGDE
EEHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCC
>Mature Secondary Structure 
TFNDPTSSIGTLAYEATIADLQAAMAAGTLTAEALTMACLERINALNRAGPCLNALIEV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
SPSALETAIALDAERDVRGPRSPLHGIPIVLKDNIDTLDDTATTAGSLALIGSRPAAEAT
CCHHHHHHHHCCCHHCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCHHHH
VAARLRAAGAVLLGKANLSEWANFRSTSSSSGWSARGGQARNPYVLSRSPCGSSSGSAIA
HHHHHHHCCEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHH
VAASMCVAAIGTETDGSISCPSAMCGVVGIKPTVGLTSRAGVIPISSTQDTVGPHARCVA
HHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHH
DAATVLGIIAGPDPHDPATTAAAGHVRPDYRTCLQADALRGARIGVLRSDRFAGFGRHVE
HHHHHHHEECCCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHH
QAFAAALTAMRDAGAHVVDPVTFPDELLAFNEAELTVLLYEFKATLNRYLASRVPDPQAA
HHHHHHHHHHHHCCCCEECCCCCCHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCC
TPAPRSLEELIAFNEQQAEHELRFFGQELLVQAAAVGDLDDPAYQQALAASRDATRQALD
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
ALLYEQQLDALVAPATGLAWPIDLIGGDRYPGGSSSLAARAGYPMVTVPAGMAFGLPIAI
HHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCHHHCCCCEEEECCCCHHCCCEEE
NFIGTAWSEPMLIRLAYAFEQATKLRRPPVYRQWIVGDE
EEHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA