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The map label for this gene is tetA [H]

Identifier: 222524983

GI number: 222524983

Start: 2166359

End: 2167642

Strand: Direct

Name: tetA [H]

Synonym: Chy400_1718

Alternate gene names: 222524983

Gene position: 2166359-2167642 (Clockwise)

Preceding gene: 222524974

Following gene: 222524985

Centisome position: 41.12

GC content: 55.69

Gene sequence:

>1284_bases
ATGAAATCACGCTCGCCGCTTGTCTTTATCTTTCTGACCATTTTCATTGATCTGCTCGGCATCGGTATCGTCTTACCGTT
GCTGCCTGAATATGTCAAAATTGTTGAACAATCAACATGGCCGTGGCTGGCCGAAAATCGAGCATTCATTGTCGGGGCGT
TAACCGCTTCGTATGCGTTGATGCAATTTCTCTTCGCCCCGGTTTTGGGCGCACTTGGTGATCGCTTTGGCCGACGGCCG
GTACTACTTCTGAGCCTGGTTGGCGCTGGGGTGAGCTATCTGGTCTTTGCTCTGGCCGAACAGTTAACGTTTCTCGGTGT
TGAGACGGTGATCGGGCTGTTGTTTCTGGCCCGCATCGCCGCCGGAATCACCGGTGCCAGCATCAGTACAGCTCAGGCAT
ATATTGCCGACGTCACACCACCAAACGAGCGTGCTCGTGGTTTGGGAATGATTGGTGCCGCCTTCGGTCTTGGCTTTATG
CTTGGCCCGGCCCTGGGCGGGTTGCTCGCTAATGTCAGTTTGCACGCCCCAGCCCTCTTCGCAGCAGCGCTCAGCTTTGC
CAACGCGACCTTCGGCTTCTTTCGTCTGCCTGAGTCGCTGCCACCGGAAAAGCGAGTACAGTCGCACGTCCGTGATCTCA
ATCCCATCAAGCGGTTGCTGGCCGTCGCCGGTGATCAGCGTGTTCAGCCATTCATTCTTGGTAGCGTCCTGTTCAATCTG
GCCTTTGCCGGACTGCAAAGCAATTTTCCGGTCTATAGCGACGAACGTTTTGGTTTCAGTCCACAGCAAAATGCGTTTGT
ATTCGCATTTATTGGCCTGATTGCAGTCGTTGTGCAGGGATTTTTGATCAGGAAGCTAGTGGCCCGCTTCGGTGAAGCCC
GCCTGACGATAGCCGGTCTGATTCTGATGGCAATTGGCTTCGCGGCTACCGGTCTGGCTTCCGCCGGATGGATGCTCTTC
CCGGCTATTGGTCTGGTGGCTCTCGGTGGCGGTATGGTCACCCCGTCACTGACAAGTCTGGTATCGCAGTCGGTATCAGC
CCAGGAGCAAGGCGCGACCCTGGGTGGTGTGCAGTCGTACAACAGTTTGATGATGGTAGCCGGGCCGTTGCTGGCCGGGA
TGCTATTCGATCTGGTTGGACAGACTGCACCCTATTTGATCGGTGCCGGTCTGCTGGCAGCTTCGCTGGTGGTGTTGTAT
GGTACGTTACGTGAGCGGTTTACCGGGACACCACAGCCGACTACTACCACCGTTTCTACCGAGACGATGGTGCAGGTTGA
GTAA

Upstream 100 bases:

>100_bases
AATCTGTGCAAAGTATTGACAAAAACTATGAATCGTCATATCATTTGCATGGCAAACTTTTCGACTTGCTATTTACTTCG
ATAGATCGGGAACTGGCACT

Downstream 100 bases:

>100_bases
CTCTCCTCAGCTCAGCACCATTGATCATGAGTGAGCAGGCACCAGACAAGGCCAATCACATCCTTGCCGGGAACCTGTTG
TGAGCGTTGTAGCCTGGATG

Product: major facilitator superfamily protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: TetA(C) [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MKSRSPLVFIFLTIFIDLLGIGIVLPLLPEYVKIVEQSTWPWLAENRAFIVGALTASYALMQFLFAPVLGALGDRFGRRP
VLLLSLVGAGVSYLVFALAEQLTFLGVETVIGLLFLARIAAGITGASISTAQAYIADVTPPNERARGLGMIGAAFGLGFM
LGPALGGLLANVSLHAPALFAAALSFANATFGFFRLPESLPPEKRVQSHVRDLNPIKRLLAVAGDQRVQPFILGSVLFNL
AFAGLQSNFPVYSDERFGFSPQQNAFVFAFIGLIAVVVQGFLIRKLVARFGEARLTIAGLILMAIGFAATGLASAGWMLF
PAIGLVALGGGMVTPSLTSLVSQSVSAQEQGATLGGVQSYNSLMMVAGPLLAGMLFDLVGQTAPYLIGAGLLAASLVVLY
GTLRERFTGTPQPTTTTVSTETMVQVE

Sequences:

>Translated_427_residues
MKSRSPLVFIFLTIFIDLLGIGIVLPLLPEYVKIVEQSTWPWLAENRAFIVGALTASYALMQFLFAPVLGALGDRFGRRP
VLLLSLVGAGVSYLVFALAEQLTFLGVETVIGLLFLARIAAGITGASISTAQAYIADVTPPNERARGLGMIGAAFGLGFM
LGPALGGLLANVSLHAPALFAAALSFANATFGFFRLPESLPPEKRVQSHVRDLNPIKRLLAVAGDQRVQPFILGSVLFNL
AFAGLQSNFPVYSDERFGFSPQQNAFVFAFIGLIAVVVQGFLIRKLVARFGEARLTIAGLILMAIGFAATGLASAGWMLF
PAIGLVALGGGMVTPSLTSLVSQSVSAQEQGATLGGVQSYNSLMMVAGPLLAGMLFDLVGQTAPYLIGAGLLAASLVVLY
GTLRERFTGTPQPTTTTVSTETMVQVE
>Mature_427_residues
MKSRSPLVFIFLTIFIDLLGIGIVLPLLPEYVKIVEQSTWPWLAENRAFIVGALTASYALMQFLFAPVLGALGDRFGRRP
VLLLSLVGAGVSYLVFALAEQLTFLGVETVIGLLFLARIAAGITGASISTAQAYIADVTPPNERARGLGMIGAAFGLGFM
LGPALGGLLANVSLHAPALFAAALSFANATFGFFRLPESLPPEKRVQSHVRDLNPIKRLLAVAGDQRVQPFILGSVLFNL
AFAGLQSNFPVYSDERFGFSPQQNAFVFAFIGLIAVVVQGFLIRKLVARFGEARLTIAGLILMAIGFAATGLASAGWMLF
PAIGLVALGGGMVTPSLTSLVSQSVSAQEQGATLGGVQSYNSLMMVAGPLLAGMLFDLVGQTAPYLIGAGLLAASLVVLY
GTLRERFTGTPQPTTTTVSTETMVQVE

Specific function: Resistance to tetracycline by an active tetracycline efflux. This is an energy-dependent process that decreases the accumulation of the antibiotic in whole cells. This protein functions as a metal-tetracycline/H(+) antiporter [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Homo sapiens, GI225703104, Length=364, Percent_Identity=32.6923076923077, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI225703102, Length=364, Percent_Identity=32.6923076923077, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI39753965, Length=380, Percent_Identity=27.1052631578947, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI94721311, Length=324, Percent_Identity=28.0864197530864, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI217416366, Length=420, Percent_Identity=25.4761904761905, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI166064062, Length=359, Percent_Identity=28.4122562674095, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI166064060, Length=359, Percent_Identity=28.4122562674095, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1787291, Length=423, Percent_Identity=25.0591016548463, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17551570, Length=370, Percent_Identity=27.2972972972973, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71996331, Length=344, Percent_Identity=27.6162790697674, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71985371, Length=374, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17738085, Length=365, Percent_Identity=29.3150684931507, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI21357569, Length=328, Percent_Identity=28.9634146341463, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI161080926, Length=328, Percent_Identity=28.9634146341463, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI161080924, Length=328, Percent_Identity=28.9634146341463, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24656463, Length=328, Percent_Identity=28.9634146341463, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24656468, Length=328, Percent_Identity=28.9634146341463, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI221513297, Length=329, Percent_Identity=26.4437689969605, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24667557, Length=228, Percent_Identity=25.8771929824561, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24667561, Length=198, Percent_Identity=28.2828282828283, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44991; Mature: 44991

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CEEEEEC
>Mature Secondary Structure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CEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 383387; 6337373; 6307828; 1517220 [H]