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The map label for this gene is iscS [H]

Identifier: 222524845

GI number: 222524845

Start: 2005094

End: 2006260

Strand: Reverse

Name: iscS [H]

Synonym: Chy400_1572

Alternate gene names: 222524845

Gene position: 2006260-2005094 (Counterclockwise)

Preceding gene: 222524846

Following gene: 222524842

Centisome position: 38.08

GC content: 59.04

Gene sequence:

>1167_bases
ATGAATAGTCGCCTGATTTACCTTGACTACGCTGCCACAACACCGCTCGATCCACGAGTACTGGAAGCGATGATGCCATT
CCTGACCGGCATGAGTGGAAACGCATCCAGCATCCATCAGGTTGGCCGGGCTGCATTGCAGGCGTTGGACGATGCGCGTG
AGCAGGTAGCGCTGGTGTTAGGGTGTCAGCCAAAAGAGATTGTCTTTACCGGCGGCGGGAGTGAAAGCATTAATCTGGCC
ATCAAAGGGGTGGCCATGGCGCTACGGGCCCAGGGCAAAACCCATCTCATTAGCAGTACCATCGAACATCACGCCGTTCT
CCATGCACTCGACTACCTGGTGGAATATGAGGGGTTTACGGTAACCCTGCTACCGGTTGATCGCACCGGTCGCGTCCAAC
CTGCCGATCTCAGTACTGCAATCCGCCCCGAAACCGCGCTCGTCTCGGTGATGTATGCCAACAATGAGACGGGTGTCATC
CAGCCGATCACCGAGCTGGCAGCCATCTGCCGCGAGCGTGGCGTGCTCTTTCACACGGATGCAGTGCAGGCACCCGGCCA
GTTACCGCTGGATGTGCATGCGTTGGGGGTTGACCTCCTGAGTCTAACCGCCCACAAGTTTTACGGGCCGCAGGGGGTAG
GTGTATTGTATCTGCGCCGGGGAACACCGCTGGTGCCACAGATCAACGGTGGCGCTCAGGAACGCCGCCGCCGGGCCGGT
ACGGAGAACATCGCCGGTATTGTGGGGTTGGCCAAAGCCTTGAGCATTGCCGAGAGCGAGCGTACCGCGCACGCGAATCG
ATTACGAGCATTGAGTGAACGCCTGATTGACGGGGTGCTTACCCGCATTCCACAATCGTGGCTGAATGGCGACCGCCAGA
CCCGACTACCGGGCATCGTTAATGTAGGTTTCGCCTGTATTGAGACCGAAAGCCTCTTACTGCTGCTCGACCAGCGCGGC
ATCTGTGCCAGTTCAGGAAGTGCCTGCACCTCTGGCTCACTCGAACCGTCGCACGTCTTACTGGCAATGGGTCTCTCACC
CGACGAAGCCAACGGCTCGATCCGCTTCTCCCTGGGCCGCCAGACCACCGATGAGCAGATTGACACTCTGCTCGATCTGT
TACCCGATCTGGTCGCGCAATTGCGAACGCTGACGCCGTGTGCGTGA

Upstream 100 bases:

>100_bases
TTGCCGCCTCGCTACGAAGTGGCCGAAATACTCAATCTCCAGGTGCTACGCGACGAAGCACGTACCCGTCGTGCTCAATA
ACCTTGCCGGAGAACCGTCT

Downstream 100 bases:

>100_bases
TGCCGGGAGTGCATACCTGGCGTGCATTGTTTCTATGCTCTCTGTTTGATGAGAATATCTGCCCACCCATCAATCAAACC
GGCAAAGGCTAGCGGGACAC

Product: cysteine desulfurase NifS

Products: NA

Alternate protein names: NifS protein homolog [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MNSRLIYLDYAATTPLDPRVLEAMMPFLTGMSGNASSIHQVGRAALQALDDAREQVALVLGCQPKEIVFTGGGSESINLA
IKGVAMALRAQGKTHLISSTIEHHAVLHALDYLVEYEGFTVTLLPVDRTGRVQPADLSTAIRPETALVSVMYANNETGVI
QPITELAAICRERGVLFHTDAVQAPGQLPLDVHALGVDLLSLTAHKFYGPQGVGVLYLRRGTPLVPQINGGAQERRRRAG
TENIAGIVGLAKALSIAESERTAHANRLRALSERLIDGVLTRIPQSWLNGDRQTRLPGIVNVGFACIETESLLLLLDQRG
ICASSGSACTSGSLEPSHVLLAMGLSPDEANGSIRFSLGRQTTDEQIDTLLDLLPDLVAQLRTLTPCA

Sequences:

>Translated_388_residues
MNSRLIYLDYAATTPLDPRVLEAMMPFLTGMSGNASSIHQVGRAALQALDDAREQVALVLGCQPKEIVFTGGGSESINLA
IKGVAMALRAQGKTHLISSTIEHHAVLHALDYLVEYEGFTVTLLPVDRTGRVQPADLSTAIRPETALVSVMYANNETGVI
QPITELAAICRERGVLFHTDAVQAPGQLPLDVHALGVDLLSLTAHKFYGPQGVGVLYLRRGTPLVPQINGGAQERRRRAG
TENIAGIVGLAKALSIAESERTAHANRLRALSERLIDGVLTRIPQSWLNGDRQTRLPGIVNVGFACIETESLLLLLDQRG
ICASSGSACTSGSLEPSHVLLAMGLSPDEANGSIRFSLGRQTTDEQIDTLLDLLPDLVAQLRTLTPCA
>Mature_388_residues
MNSRLIYLDYAATTPLDPRVLEAMMPFLTGMSGNASSIHQVGRAALQALDDAREQVALVLGCQPKEIVFTGGGSESINLA
IKGVAMALRAQGKTHLISSTIEHHAVLHALDYLVEYEGFTVTLLPVDRTGRVQPADLSTAIRPETALVSVMYANNETGVI
QPITELAAICRERGVLFHTDAVQAPGQLPLDVHALGVDLLSLTAHKFYGPQGVGVLYLRRGTPLVPQINGGAQERRRRAG
TENIAGIVGLAKALSIAESERTAHANRLRALSERLIDGVLTRIPQSWLNGDRQTRLPGIVNVGFACIETESLLLLLDQRG
ICASSGSACTSGSLEPSHVLLAMGLSPDEANGSIRFSLGRQTTDEQIDTLLDLLPDLVAQLRTLTPCA

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=390, Percent_Identity=46.9230769230769, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI156713448, Length=413, Percent_Identity=37.046004842615, Blast_Score=245, Evalue=5e-65,
Organism=Escherichia coli, GI48994898, Length=387, Percent_Identity=48.578811369509, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI1787970, Length=389, Percent_Identity=26.2210796915167, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1789175, Length=392, Percent_Identity=28.0612244897959, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI25143064, Length=385, Percent_Identity=47.5324675324675, Blast_Score=343, Evalue=6e-95,
Organism=Caenorhabditis elegans, GI17533177, Length=320, Percent_Identity=32.5, Blast_Score=153, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6319831, Length=387, Percent_Identity=45.7364341085271, Blast_Score=325, Evalue=9e-90,
Organism=Drosophila melanogaster, GI20129463, Length=386, Percent_Identity=46.6321243523316, Blast_Score=321, Evalue=6e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 41484; Mature: 41484

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSRLIYLDYAATTPLDPRVLEAMMPFLTGMSGNASSIHQVGRAALQALDDAREQVALVL
CCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEE
GCQPKEIVFTGGGSESINLAIKGVAMALRAQGKTHLISSTIEHHAVLHALDYLVEYEGFT
CCCCCEEEEECCCCCEEEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
VTLLPVDRTGRVQPADLSTAIRPETALVSVMYANNETGVIQPITELAAICRERGVLFHTD
EEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEC
AVQAPGQLPLDVHALGVDLLSLTAHKFYGPQGVGVLYLRRGTPLVPQINGGAQERRRRAG
CCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCC
TENIAGIVGLAKALSIAESERTAHANRLRALSERLIDGVLTRIPQSWLNGDRQTRLPGIV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHH
NVGFACIETESLLLLLDQRGICASSGSACTSGSLEPSHVLLAMGLSPDEANGSIRFSLGR
HCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECC
QTTDEQIDTLLDLLPDLVAQLRTLTPCA
CCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNSRLIYLDYAATTPLDPRVLEAMMPFLTGMSGNASSIHQVGRAALQALDDAREQVALVL
CCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEE
GCQPKEIVFTGGGSESINLAIKGVAMALRAQGKTHLISSTIEHHAVLHALDYLVEYEGFT
CCCCCEEEEECCCCCEEEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
VTLLPVDRTGRVQPADLSTAIRPETALVSVMYANNETGVIQPITELAAICRERGVLFHTD
EEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEC
AVQAPGQLPLDVHALGVDLLSLTAHKFYGPQGVGVLYLRRGTPLVPQINGGAQERRRRAG
CCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHCC
TENIAGIVGLAKALSIAESERTAHANRLRALSERLIDGVLTRIPQSWLNGDRQTRLPGIV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHH
NVGFACIETESLLLLLDQRGICASSGSACTSGSLEPSHVLLAMGLSPDEANGSIRFSLGR
HCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECC
QTTDEQIDTLLDLLPDLVAQLRTLTPCA
CCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10930739 [H]