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The map label for this gene is glnA [H]

Identifier: 222524844

GI number: 222524844

Start: 2003185

End: 2004606

Strand: Direct

Name: glnA [H]

Synonym: Chy400_1571

Alternate gene names: 222524844

Gene position: 2003185-2004606 (Clockwise)

Preceding gene: 222524843

Following gene: 222524847

Centisome position: 38.02

GC content: 55.41

Gene sequence:

>1422_bases
ATGACGATGGATGCGAAGGGCGTTATCGAATTGATCAAAGCCAAGGGAATTCAAATAGTTGATACCCGCTTTACCGATCT
CTTTGGTGGGTGGCAGCACTATTCGCTGCCGGCCTCGCGGCTGACTGAAGATATGATTCGCGAGGGTTTGGGCTTTGATG
GGTCGTCGATCAAGGGTTTTCAAGCCATCAACGAGAGCGATATGTTGATGGTGCCCGATCCATCAACGGCATTTATCGAT
CCCACCCTCAGTGTGCCAACACTGGTACTGATCTGTGACATTATCGATCCCATCACCCGCCAGCCCTACAGCCGCGACCC
GCGCACGGTTGCTAAAAAGGCCGAGGCCTATCTCAAGACGACCGGTATTGCCGATACCGCCTACTTCGGGCCGGAAGCTG
AATTCTTCCTCTTCAGTGATGTACGCTTCGGTCAGGGAGCCAACCACGGTTTCTACTATATCGACAGCCCGGAAGCGGTC
TGGAATACCGGCGCTGAAATACCGGGTGGCAACAAGGGCTACCGCATCCGCCACAAAGAGGGCTACTTCCCGGTTCCACC
CACCGATACCCTTCAGGATATTCGCTCAGAGATGATCCTGAAGATGATGGAGATCGGGATCGAGATTGAGCTGCACCATC
ACGAAGTGGCAACCGCCGGTCAGTGCGAGATCGATATGCGCTTTGACTCGCTGGTCAATATGGCCGACAAGGTGCAGAAG
TACAAGTATATTGTGCGCAATGTCGCTCGTGCGCACGGCTACAGTGCTACCTTTATGCCAAAGCCGATCTTCGGCGACAA
TGGATCGGGTATGCACACCCACCAAAGCCTCTGGAAAGATGGCGAGCCGCTCTTCTTCGATGAGACGCAATACGCTCTGC
TCTCGAAGACGGCCCAGTACTACATCGGCGGTATTCTCAAGCACGCTCCGGCGCTGCTGGCAATTTGTGCCCCGACCACC
AATAGCTATCGCCGGCTGGTACCGGGCTTCGAGGCTCCGATCAATCTTGTCTACTCGATGCGCAATCGCTCGGCGGCGAT
CCGTATTCCGACCTATAGCTCATCACCCAAGGCGCGCCGTATCGAATTCCGGGCGCCGGATGCGATGTGCAACCCGTATC
TCGCCTTCGCTGCTATGATGCTGGCCGGTCTCGACGGTATCCAGAACAAGATTGAGCCGCCACCGCCGCTCGATGTTGAC
ATCTACGAACTCTCACCGGAAGAGAAGGGCGACATTCGCAGCACACCCGGCTCGCTCACCGAAGCCCTTGATGCACTCGA
ACAGGATTATCACTTCCTGCTCAAGGGCAATGTCTTCACGACCGATCTGCTCGAAACGTACATCGAAACCAAGCGCAAAG
AGGCTGTCGGTCTGTCGCTGCGCCCGCATCCGTATGAGTTCATGATGTATTACGACGCTTAA

Upstream 100 bases:

>100_bases
GCCGCTGGTCACAAATGTACCACGGCGTCGTTGCCTGTTTTAGGACCACGTTCGTTCTGCAGGACCGCGTTCGTTCTAGT
GTAGCAAGGAGTAGACTTGT

Downstream 100 bases:

>100_bases
TAGCCCGCTACATACGCAACAGAAGGGGCGCATTCCAGGTGCGCCCCTTCTGTTTTGCTCACGCGAGCCTGCTATGAATA
CCGCCCCCATCCGAAGCATT

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MTMDAKGVIELIKAKGIQIVDTRFTDLFGGWQHYSLPASRLTEDMIREGLGFDGSSIKGFQAINESDMLMVPDPSTAFID
PTLSVPTLVLICDIIDPITRQPYSRDPRTVAKKAEAYLKTTGIADTAYFGPEAEFFLFSDVRFGQGANHGFYYIDSPEAV
WNTGAEIPGGNKGYRIRHKEGYFPVPPTDTLQDIRSEMILKMMEIGIEIELHHHEVATAGQCEIDMRFDSLVNMADKVQK
YKYIVRNVARAHGYSATFMPKPIFGDNGSGMHTHQSLWKDGEPLFFDETQYALLSKTAQYYIGGILKHAPALLAICAPTT
NSYRRLVPGFEAPINLVYSMRNRSAAIRIPTYSSSPKARRIEFRAPDAMCNPYLAFAAMMLAGLDGIQNKIEPPPPLDVD
IYELSPEEKGDIRSTPGSLTEALDALEQDYHFLLKGNVFTTDLLETYIETKRKEAVGLSLRPHPYEFMMYYDA

Sequences:

>Translated_473_residues
MTMDAKGVIELIKAKGIQIVDTRFTDLFGGWQHYSLPASRLTEDMIREGLGFDGSSIKGFQAINESDMLMVPDPSTAFID
PTLSVPTLVLICDIIDPITRQPYSRDPRTVAKKAEAYLKTTGIADTAYFGPEAEFFLFSDVRFGQGANHGFYYIDSPEAV
WNTGAEIPGGNKGYRIRHKEGYFPVPPTDTLQDIRSEMILKMMEIGIEIELHHHEVATAGQCEIDMRFDSLVNMADKVQK
YKYIVRNVARAHGYSATFMPKPIFGDNGSGMHTHQSLWKDGEPLFFDETQYALLSKTAQYYIGGILKHAPALLAICAPTT
NSYRRLVPGFEAPINLVYSMRNRSAAIRIPTYSSSPKARRIEFRAPDAMCNPYLAFAAMMLAGLDGIQNKIEPPPPLDVD
IYELSPEEKGDIRSTPGSLTEALDALEQDYHFLLKGNVFTTDLLETYIETKRKEAVGLSLRPHPYEFMMYYDA
>Mature_472_residues
TMDAKGVIELIKAKGIQIVDTRFTDLFGGWQHYSLPASRLTEDMIREGLGFDGSSIKGFQAINESDMLMVPDPSTAFIDP
TLSVPTLVLICDIIDPITRQPYSRDPRTVAKKAEAYLKTTGIADTAYFGPEAEFFLFSDVRFGQGANHGFYYIDSPEAVW
NTGAEIPGGNKGYRIRHKEGYFPVPPTDTLQDIRSEMILKMMEIGIEIELHHHEVATAGQCEIDMRFDSLVNMADKVQKY
KYIVRNVARAHGYSATFMPKPIFGDNGSGMHTHQSLWKDGEPLFFDETQYALLSKTAQYYIGGILKHAPALLAICAPTTN
SYRRLVPGFEAPINLVYSMRNRSAAIRIPTYSSSPKARRIEFRAPDAMCNPYLAFAAMMLAGLDGIQNKIEPPPPLDVDI
YELSPEEKGDIRSTPGSLTEALDALEQDYHFLLKGNVFTTDLLETYIETKRKEAVGLSLRPHPYEFMMYYDA

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=371, Percent_Identity=24.7978436657682, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI74271826, Length=347, Percent_Identity=27.3775216138329, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI74271837, Length=347, Percent_Identity=27.3775216138329, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI19923206, Length=347, Percent_Identity=27.3775216138329, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1790301, Length=469, Percent_Identity=54.3710021321962, Blast_Score=523, Evalue=1e-149,
Organism=Escherichia coli, GI87081870, Length=414, Percent_Identity=27.2946859903382, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 53026; Mature: 52895

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMDAKGVIELIKAKGIQIVDTRFTDLFGGWQHYSLPASRLTEDMIREGLGFDGSSIKGF
CCCCHHHHHHHHHHCCCEEEECHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHH
QAINESDMLMVPDPSTAFIDPTLSVPTLVLICDIIDPITRQPYSRDPRTVAKKAEAYLKT
EECCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
TGIADTAYFGPEAEFFLFSDVRFGQGANHGFYYIDSPEAVWNTGAEIPGGNKGYRIRHKE
CCCCCCCCCCCCCCEEEEECEECCCCCCCCEEEECCCHHHHCCCCCCCCCCCCEEEEECC
GYFPVPPTDTLQDIRSEMILKMMEIGIEIELHHHEVATAGQCEIDMRFDSLVNMADKVQK
CCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHH
YKYIVRNVARAHGYSATFMPKPIFGDNGSGMHTHQSLWKDGEPLFFDETQYALLSKTAQY
HHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCHHHHHHCCCCCEEECCHHHHHHHHHHHH
YIGGILKHAPALLAICAPTTNSYRRLVPGFEAPINLVYSMRNRSAAIRIPTYSSSPKARR
HHHHHHHHCCEEEEEECCCCCCHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCEE
IEFRAPDAMCNPYLAFAAMMLAGLDGIQNKIEPPPPLDVDIYELSPEEKGDIRSTPGSLT
EEEECCCHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCHHHH
EALDALEQDYHFLLKGNVFTTDLLETYIETKRKEAVGLSLRPHPYEFMMYYDA
HHHHHHHHCCEEEEECCEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECC
>Mature Secondary Structure 
TMDAKGVIELIKAKGIQIVDTRFTDLFGGWQHYSLPASRLTEDMIREGLGFDGSSIKGF
CCCHHHHHHHHHHCCCEEEECHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHH
QAINESDMLMVPDPSTAFIDPTLSVPTLVLICDIIDPITRQPYSRDPRTVAKKAEAYLKT
EECCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
TGIADTAYFGPEAEFFLFSDVRFGQGANHGFYYIDSPEAVWNTGAEIPGGNKGYRIRHKE
CCCCCCCCCCCCCCEEEEECEECCCCCCCCEEEECCCHHHHCCCCCCCCCCCCEEEEECC
GYFPVPPTDTLQDIRSEMILKMMEIGIEIELHHHEVATAGQCEIDMRFDSLVNMADKVQK
CCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHH
YKYIVRNVARAHGYSATFMPKPIFGDNGSGMHTHQSLWKDGEPLFFDETQYALLSKTAQY
HHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCHHHHHHCCCCCEEECCHHHHHHHHHHHH
YIGGILKHAPALLAICAPTTNSYRRLVPGFEAPINLVYSMRNRSAAIRIPTYSSSPKARR
HHHHHHHHCCEEEEEECCCCCCHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCEE
IEFRAPDAMCNPYLAFAAMMLAGLDGIQNKIEPPPPLDVDIYELSPEEKGDIRSTPGSLT
EEEECCCHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCHHHH
EALDALEQDYHFLLKGNVFTTDLLETYIETKRKEAVGLSLRPHPYEFMMYYDA
HHHHHHHHCCEEEEECCEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7678591 [H]