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The map label for this gene is ada [H]

Identifier: 222524801

GI number: 222524801

Start: 1947408

End: 1947971

Strand: Reverse

Name: ada [H]

Synonym: Chy400_1528

Alternate gene names: 222524801

Gene position: 1947971-1947408 (Counterclockwise)

Preceding gene: 222524802

Following gene: 222524799

Centisome position: 36.97

GC content: 59.57

Gene sequence:

>564_bases
ATGCCAAATCACGACTCTCAAGCACGAGATGATAAACGTGCTGCTCGTGTCAGACCGATACGAGAGGTTGCATATGCAGA
TAGCCCCCTCGGTCGAGTGTTGGTAGCTGCGACCGATAGCGGTCTCTGTGCGGTCTATTTAGGTGATGATGACGCAGCAC
TGCTGGCTACACTCCACGCTAGCTTTCCGGTAGCGGGCACTGCCGCTGGTGTTCTGGCGGAGACTGCGGCATCGGCAATT
GCAGCCTATCTGCGCAACGAGGCACCGCTCCCCGCCTTCCCACTTGATCCAATTGGTACCCCCTTTCAACAACTGGTCTG
GCAGGCGCTTTGTACGATCCCGCGTGGGAGCACCCGTACCTACGGTCAGCTAGCTCAGGAGTTAGGATTACCAACCGGCG
CTGCACGGGCAGTCGGACGGGCCTGTGCGAGCAATTCACTGGCGATAGTGGTGCCCTGTCATCGTGCCCTGGGGAGCGAT
GGCAGGCTCCATGGCTTTCGCTGGGGGATCGAGCGCAAGCGTGCGTTGCTGGCCCTTGAAGGGGTATTCCTGCCGCTTCT
GTAG

Upstream 100 bases:

>100_bases
TGGGCTTGTCTGGCAAACGATGGTTGAATACGTGCCAGAGACATATCGCACGGGGTTGCAGTCGGGTGATGCTGGCAATA
GCTGATCGGAAGTTGCCCCC

Downstream 100 bases:

>100_bases
TATCAAATCTGGTGCTACAGCGGTCATACATGCTGGTACCGCACCTGATCACAGAGGCGTGGAACCAGTGTAATCGCTGT
ACGTGTCGTGGGATCACACG

Product: methylated-DNA/protein-cysteine methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 187; Mature: 186

Protein sequence:

>187_residues
MPNHDSQARDDKRAARVRPIREVAYADSPLGRVLVAATDSGLCAVYLGDDDAALLATLHASFPVAGTAAGVLAETAASAI
AAYLRNEAPLPAFPLDPIGTPFQQLVWQALCTIPRGSTRTYGQLAQELGLPTGAARAVGRACASNSLAIVVPCHRALGSD
GRLHGFRWGIERKRALLALEGVFLPLL

Sequences:

>Translated_187_residues
MPNHDSQARDDKRAARVRPIREVAYADSPLGRVLVAATDSGLCAVYLGDDDAALLATLHASFPVAGTAAGVLAETAASAI
AAYLRNEAPLPAFPLDPIGTPFQQLVWQALCTIPRGSTRTYGQLAQELGLPTGAARAVGRACASNSLAIVVPCHRALGSD
GRLHGFRWGIERKRALLALEGVFLPLL
>Mature_186_residues
PNHDSQARDDKRAARVRPIREVAYADSPLGRVLVAATDSGLCAVYLGDDDAALLATLHASFPVAGTAAGVLAETAASAIA
AYLRNEAPLPAFPLDPIGTPFQQLVWQALCTIPRGSTRTYGQLAQELGLPTGAARAVGRACASNSLAIVVPCHRALGSDG
RLHGFRWGIERKRALLALEGVFLPLL

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=162, Percent_Identity=30.2469135802469, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1788542, Length=159, Percent_Identity=49.685534591195, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1787596, Length=121, Percent_Identity=44.6280991735537, Blast_Score=95, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI115533068, Length=171, Percent_Identity=38.5964912280702, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI115533070, Length=131, Percent_Identity=40.4580152671756, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6320001, Length=114, Percent_Identity=46.4912280701754, Blast_Score=102, Evalue=3e-23,
Organism=Drosophila melanogaster, GI17137554, Length=89, Percent_Identity=48.314606741573, Blast_Score=67, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19647; Mature: 19516

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNHDSQARDDKRAARVRPIREVAYADSPLGRVLVAATDSGLCAVYLGDDDAALLATLHA
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCHHHHHHHHH
SFPVAGTAAGVLAETAASAIAAYLRNEAPLPAFPLDPIGTPFQQLVWQALCTIPRGSTRT
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHH
YGQLAQELGLPTGAARAVGRACASNSLAIVVPCHRALGSDGRLHGFRWGIERKRALLALE
HHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECHHHCCCCCCCCHHHHCHHHHHHHHHHH
GVFLPLL
HHHHCCC
>Mature Secondary Structure 
PNHDSQARDDKRAARVRPIREVAYADSPLGRVLVAATDSGLCAVYLGDDDAALLATLHA
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCHHHHHHHHH
SFPVAGTAAGVLAETAASAIAAYLRNEAPLPAFPLDPIGTPFQQLVWQALCTIPRGSTRT
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHH
YGQLAQELGLPTGAARAVGRACASNSLAIVVPCHRALGSDGRLHGFRWGIERKRALLALE
HHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECHHHCCCCCCCCHHHHCHHHHHHHHHHH
GVFLPLL
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]