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The map label for this gene is nadE [H]

Identifier: 222524771

GI number: 222524771

Start: 1894441

End: 1896534

Strand: Reverse

Name: nadE [H]

Synonym: Chy400_1498

Alternate gene names: 222524771

Gene position: 1896534-1894441 (Counterclockwise)

Preceding gene: 222524776

Following gene: 222524770

Centisome position: 35.99

GC content: 59.46

Gene sequence:

>2094_bases
ATGCAACCATTCTTCTCGATCTATCGCCACAATCTGGCCCGATTAGCGGTGGCTATTCCCGCAGTACGGATTGCCGAACC
GGTCTTCAACGCTGAACGAACGATTGCTCTGGCCCGGCGTGCTGCGGCTGAAGGGGCAATTCTCGTGCTGTTCCCCGAAC
TCGGCATCTCGGCCTACAGCAACGATGATCTCTTTCAGCAGTCAACCCTCCTCGATGCGGTTGAGCAGGCACTACTCACG
ATTGCTGCGGCGGCCAACGACATCAATGCCCTGCTGCTCGTCGGCGCTCCCCTTCGGCACGAAGGCCGGCTTTTCAACTG
TGCGGTAGCCATCTACCGCGGACAGATTATTGGCGTTACGCCGAAGAGTTATTTACCGAATTACCGGGAATTTTACGAAA
AACGCCATTTTGCCGCTGCCCGTGATGCTATCGGTGACACTATCCAACTGGCCGGTCAGAACGTACCTTTTGGCACCGAT
GTAATCTATGACGTTACAACCCTGCCCGGTCTTGCTGTACACGTTGAAATTTGTGAGGATTTGTGGACGCCGCTCCCACC
CAGTACCTTTGCGGCGCTAGCCGGGGCGACAGTTCTCTGCAATCTGTCGGCATCAAACATCACGATTGGCAAAGCCGATT
ACCGGCGGGCGTTGTGTATGAATCAATCGGCGCGCACCCTTGCCGCCTACCTCTACTCGGCTGCCGGGCCGGGTGAGAGT
ACGACCGACCTGGCGTGGGATGGACACGCGCTGATCTGCGAAAACGGCGAGCTGTTGGCCGAGTCGCAGCGCTTCTGCGA
CGAGGAACAGATCATTTTTGCCGATGTCGATCTGGAACGCCTGCTTCAGGAGCGCGCCCGCCAGACCAGCTTTAGCGACA
GTATTGGCGACTACCGCTCACACGTGCGTCACATGCGCCGTATCCCGCTCGCCATCACCCTGCCGGTTGTGACCGACCTG
CGGCGCAGTGTTGCCCGCTTCCCCTTTGTGCCCAACGATCCGGCGACCCGTGATGAACGCTGCTACGAAGCCTACAACAT
CCAGATGCACGGTCTCATGCAGCGCATGCGCAGTGCTGGAGTGGAAAAAGTGGTGATTGGTGTTTCGGGCGGTCTCGACA
GCACCCAGGCGCTGATCGTCGCGGCCCGCACGATGGATCGGCTGGGGTTGCCGCGTCAGAATGTCCTGGCCTACACGATG
CCCGGCTTTGCGACCAGCAGCCAAACCCGGCGCAACGCGCACGCGCTGATGACAGCGCTCGGCGTGAGTGCGCATGAAAT
TGACATCCGCCCCTCGGCGTTGCAAATGCTCCGCGATCTGGGCCATCCGGCAGGCGAAGGCGCAGCCGTCTACGATGTCA
CGTTTGAAAATGTACAGGCCGGCGAACGAACCTCGCATCTGTTTCGCCTCGCCAACTACCACAATGCGATTGTCGTCGGC
ACCGGTGATCTGAGTGAATTAGCACTCGGCTGGTGTACCTACGGAGTAGGCGATCAGATGGCGCACTACAACGTCAATGC
GTCGGTGCCGAAAACCCTCATCCAGCACCTGATTCGCTACGTCATCCGCAGCGGCGAACTGGGAGACGAAGCCAACCGGG
TGCTAGAGTCAATTCTGGCGACTGAAATCTCGCCTGAGCTGGTACCGGCTTCGAGCAGCGACGACAAACCGGCGCAACGC
ACCGAAGAGATTATCGGGCCATACGCGCTTCAGGATTTCAACCTCTACTACATCACCCGCTACGGCTTTCGTCCGGGTAA
AGTTGCCTTCCTCGCCATGCACGCCTGGGGTAATGCCGACGTTGGTGCCTGGCCAGAGGGGTTACCGGCAAACGAGCGGC
GCGCTTACGACCTGCCGACCATCCTGCACTGGTTACGGGTCTTCCTGTTCCGCTTCTTCCAGATCAGCCAGTTCAAGCGG
TCGGCCATGCCCAACGGTCCCAAAGTCGGCTCAGGTGGCTCGCTCTCGCCGCGCGGCGATTGGCGGGCACCGAGTGATGC
GCGGGCAACGGTGTGGCTGGCTGAACTTGATCGGGTGGTGGCGGAGGTGGGGGATACGCTTGAGAAAAAGCATTGGGATG
TGCGAAATGCATGA

Upstream 100 bases:

>100_bases
GAAATCACCTTTCTGGCAGGCCGACATTTCTTTCAGGTAACGGCTATCCTGATAGCACGCCAACCTGTTCATTAATCGCA
ACACAGCCGTGGATGATTAT

Downstream 100 bases:

>100_bases
TCGCACCGATCTGACGCATGAGAAAGTGAGATAATCACCAGCGAAGAGAAAATCGGTGTCATCCACGCAAAGCCATCACA
CCGTACCACAACGAGTCTGG

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 697; Mature: 697

Protein sequence:

>697_residues
MQPFFSIYRHNLARLAVAIPAVRIAEPVFNAERTIALARRAAAEGAILVLFPELGISAYSNDDLFQQSTLLDAVEQALLT
IAAAANDINALLLVGAPLRHEGRLFNCAVAIYRGQIIGVTPKSYLPNYREFYEKRHFAAARDAIGDTIQLAGQNVPFGTD
VIYDVTTLPGLAVHVEICEDLWTPLPPSTFAALAGATVLCNLSASNITIGKADYRRALCMNQSARTLAAYLYSAAGPGES
TTDLAWDGHALICENGELLAESQRFCDEEQIIFADVDLERLLQERARQTSFSDSIGDYRSHVRHMRRIPLAITLPVVTDL
RRSVARFPFVPNDPATRDERCYEAYNIQMHGLMQRMRSAGVEKVVIGVSGGLDSTQALIVAARTMDRLGLPRQNVLAYTM
PGFATSSQTRRNAHALMTALGVSAHEIDIRPSALQMLRDLGHPAGEGAAVYDVTFENVQAGERTSHLFRLANYHNAIVVG
TGDLSELALGWCTYGVGDQMAHYNVNASVPKTLIQHLIRYVIRSGELGDEANRVLESILATEISPELVPASSSDDKPAQR
TEEIIGPYALQDFNLYYITRYGFRPGKVAFLAMHAWGNADVGAWPEGLPANERRAYDLPTILHWLRVFLFRFFQISQFKR
SAMPNGPKVGSGGSLSPRGDWRAPSDARATVWLAELDRVVAEVGDTLEKKHWDVRNA

Sequences:

>Translated_697_residues
MQPFFSIYRHNLARLAVAIPAVRIAEPVFNAERTIALARRAAAEGAILVLFPELGISAYSNDDLFQQSTLLDAVEQALLT
IAAAANDINALLLVGAPLRHEGRLFNCAVAIYRGQIIGVTPKSYLPNYREFYEKRHFAAARDAIGDTIQLAGQNVPFGTD
VIYDVTTLPGLAVHVEICEDLWTPLPPSTFAALAGATVLCNLSASNITIGKADYRRALCMNQSARTLAAYLYSAAGPGES
TTDLAWDGHALICENGELLAESQRFCDEEQIIFADVDLERLLQERARQTSFSDSIGDYRSHVRHMRRIPLAITLPVVTDL
RRSVARFPFVPNDPATRDERCYEAYNIQMHGLMQRMRSAGVEKVVIGVSGGLDSTQALIVAARTMDRLGLPRQNVLAYTM
PGFATSSQTRRNAHALMTALGVSAHEIDIRPSALQMLRDLGHPAGEGAAVYDVTFENVQAGERTSHLFRLANYHNAIVVG
TGDLSELALGWCTYGVGDQMAHYNVNASVPKTLIQHLIRYVIRSGELGDEANRVLESILATEISPELVPASSSDDKPAQR
TEEIIGPYALQDFNLYYITRYGFRPGKVAFLAMHAWGNADVGAWPEGLPANERRAYDLPTILHWLRVFLFRFFQISQFKR
SAMPNGPKVGSGGSLSPRGDWRAPSDARATVWLAELDRVVAEVGDTLEKKHWDVRNA
>Mature_697_residues
MQPFFSIYRHNLARLAVAIPAVRIAEPVFNAERTIALARRAAAEGAILVLFPELGISAYSNDDLFQQSTLLDAVEQALLT
IAAAANDINALLLVGAPLRHEGRLFNCAVAIYRGQIIGVTPKSYLPNYREFYEKRHFAAARDAIGDTIQLAGQNVPFGTD
VIYDVTTLPGLAVHVEICEDLWTPLPPSTFAALAGATVLCNLSASNITIGKADYRRALCMNQSARTLAAYLYSAAGPGES
TTDLAWDGHALICENGELLAESQRFCDEEQIIFADVDLERLLQERARQTSFSDSIGDYRSHVRHMRRIPLAITLPVVTDL
RRSVARFPFVPNDPATRDERCYEAYNIQMHGLMQRMRSAGVEKVVIGVSGGLDSTQALIVAARTMDRLGLPRQNVLAYTM
PGFATSSQTRRNAHALMTALGVSAHEIDIRPSALQMLRDLGHPAGEGAAVYDVTFENVQAGERTSHLFRLANYHNAIVVG
TGDLSELALGWCTYGVGDQMAHYNVNASVPKTLIQHLIRYVIRSGELGDEANRVLESILATEISPELVPASSSDDKPAQR
TEEIIGPYALQDFNLYYITRYGFRPGKVAFLAMHAWGNADVGAWPEGLPANERRAYDLPTILHWLRVFLFRFFQISQFKR
SAMPNGPKVGSGGSLSPRGDWRAPSDARATVWLAELDRVVAEVGDTLEKKHWDVRNA

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=594, Percent_Identity=23.5690235690236, Blast_Score=99, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17538756, Length=259, Percent_Identity=29.3436293436293, Blast_Score=105, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6321865, Length=638, Percent_Identity=23.6677115987461, Blast_Score=107, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24641841, Length=647, Percent_Identity=23.6476043276662, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24641843, Length=647, Percent_Identity=23.6476043276662, Blast_Score=100, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 76720; Mature: 76720

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPFFSIYRHNLARLAVAIPAVRIAEPVFNAERTIALARRAAAEGAILVLFPELGISAYS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEECCCCCCCCC
NDDLFQQSTLLDAVEQALLTIAAAANDINALLLVGAPLRHEGRLFNCAVAIYRGQIIGVT
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEEECCEEEEEC
PKSYLPNYREFYEKRHFAAARDAIGDTIQLAGQNVPFGTDVIYDVTTLPGLAVHVEICED
CHHHCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCEEECCCCCCCEEEHHHHHH
LWTPLPPSTFAALAGATVLCNLSASNITIGKADYRRALCMNQSARTLAAYLYSAAGPGES
HCCCCCCHHHHHHCCCEEEEECCCCCEEECHHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
TTDLAWDGHALICENGELLAESQRFCDEEQIIFADVDLERLLQERARQTSFSDSIGDYRS
CCCEEECCCEEEECCCCHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHCCCHHHHHHHHH
HVRHMRRIPLAITLPVVTDLRRSVARFPFVPNDPATRDERCYEAYNIQMHGLMQRMRSAG
HHHHHHHCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
VEKVVIGVSGGLDSTQALIVAARTMDRLGLPRQNVLAYTMPGFATSSQTRRNAHALMTAL
CEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHH
GVSAHEIDIRPSALQMLRDLGHPAGEGAAVYDVTFENVQAGERTSHLFRLANYHNAIVVG
CCCCEEECCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEE
TGDLSELALGWCTYGVGDQMAHYNVNASVPKTLIQHLIRYVIRSGELGDEANRVLESILA
CCCHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
TEISPELVPASSSDDKPAQRTEEIIGPYALQDFNLYYITRYGFRPGKVAFLAMHAWGNAD
HCCCCCEECCCCCCCCHHHHHHHHHCCEEEECCCEEEEEEECCCCCCEEEEEEECCCCCC
VGAWPEGLPANERRAYDLPTILHWLRVFLFRFFQISQFKRSAMPNGPKVGSGGSLSPRGD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
WRAPSDARATVWLAELDRVVAEVGDTLEKKHWDVRNA
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQPFFSIYRHNLARLAVAIPAVRIAEPVFNAERTIALARRAAAEGAILVLFPELGISAYS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEECCCCCCCCC
NDDLFQQSTLLDAVEQALLTIAAAANDINALLLVGAPLRHEGRLFNCAVAIYRGQIIGVT
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEEECCEEEEEC
PKSYLPNYREFYEKRHFAAARDAIGDTIQLAGQNVPFGTDVIYDVTTLPGLAVHVEICED
CHHHCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCEEECCCCCCCEEEHHHHHH
LWTPLPPSTFAALAGATVLCNLSASNITIGKADYRRALCMNQSARTLAAYLYSAAGPGES
HCCCCCCHHHHHHCCCEEEEECCCCCEEECHHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
TTDLAWDGHALICENGELLAESQRFCDEEQIIFADVDLERLLQERARQTSFSDSIGDYRS
CCCEEECCCEEEECCCCHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHCCCHHHHHHHHH
HVRHMRRIPLAITLPVVTDLRRSVARFPFVPNDPATRDERCYEAYNIQMHGLMQRMRSAG
HHHHHHHCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
VEKVVIGVSGGLDSTQALIVAARTMDRLGLPRQNVLAYTMPGFATSSQTRRNAHALMTAL
CEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHH
GVSAHEIDIRPSALQMLRDLGHPAGEGAAVYDVTFENVQAGERTSHLFRLANYHNAIVVG
CCCCEEECCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCEEEEE
TGDLSELALGWCTYGVGDQMAHYNVNASVPKTLIQHLIRYVIRSGELGDEANRVLESILA
CCCHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
TEISPELVPASSSDDKPAQRTEEIIGPYALQDFNLYYITRYGFRPGKVAFLAMHAWGNAD
HCCCCCEECCCCCCCCHHHHHHHHHCCEEEECCCEEEEEEECCCCCCEEEEEEECCCCCC
VGAWPEGLPANERRAYDLPTILHWLRVFLFRFFQISQFKRSAMPNGPKVGSGGSLSPRGD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
WRAPSDARATVWLAELDRVVAEVGDTLEKKHWDVRNA
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]