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The map label for this gene is paaF [C]

Identifier: 222524741

GI number: 222524741

Start: 1863650

End: 1864456

Strand: Reverse

Name: paaF [C]

Synonym: Chy400_1468

Alternate gene names: 222524741

Gene position: 1864456-1863650 (Counterclockwise)

Preceding gene: 222524755

Following gene: 222524740

Centisome position: 35.39

GC content: 58.24

Gene sequence:

>807_bases
ATGGAAGGACACGAAGTTGCTGTCGAACCATTCGTCTTGAGCGAGATTGATGGACGTGGAGTGGTTCGCCTTACCCTCAA
CCGGCCACGCCAGTTCAATGCCCTGTCGGAAGAGATGCTCGAAGCACTGCAACGTGAACTCGATGCGATTGCCGCCAATC
CGCAGGCACGGGTGGTGGTGTTGGCGGGCGCCGGTAAAGCCTTTTGCGCCGGTCATGACCTCAAGCAGATGCAGGCGCAT
CCCGATCAGGCTTACTACGAAGACCTCTTTGCCCGTTGTACGCAGATGATGCTGACGATACAGCGCCTGCCGCAACCGGT
CATTGCACGTGTGCACGGGATTGCCACTGCCGCCGGATGCCAGTTGGTAGCAATGTGTGACCTGGCTGTTGCGTCGACTG
AAGCAAGGTTTGCCGTGTCGGGGATTACGGTTGGTCTGTTCTGTTCGACACCATCGGTTGCGCTGTCGCGCAATATCGGG
CGTAAGGCCGCTTTCGAGATGCTGGTCACCGGTGAGTTTATCGATGCCGTTACCGCACAGCGGTTAGGGCTGGTCAATCG
GGTAGCAGCACCGGCAGACCTCGATGCCGAGGTGCAGCGATTGGTGGATGCAATTTTGGCGAAGCCGGCAGAGTCGATTG
CTATCGGCAAGTCGCTCTTCTACCGCCAGCTTGAACTGCCAATGGAAGAAGCTTACCGGCTGGCCGGGCAGACGATGGCA
TGCAACATGATGGAACCGTGTGCTCAAGAGGGGGTCAGTGCATTTGTCGAGAAGCGTCCGCCGGCGTGGGCGAATGTCGA
ACGCTAA

Upstream 100 bases:

>100_bases
AGTCGAACGCGGGTTGAGATTGTGGAAAATGTGCGTGACAACCTGTCTTCGTGTACAATGGTGACAGTTACCCCAAACCA
TATTCATACGGAGGTCTACG

Downstream 100 bases:

>100_bases
CAACAGGAGCTATACTACCCACCTTTTGGTGAGTAACGCACGAAAGGTAAGCGTCCGCGAAACGATGATTGTGTGTCCCT
CCGCTCGACGAGGCTTTGCA

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MEGHEVAVEPFVLSEIDGRGVVRLTLNRPRQFNALSEEMLEALQRELDAIAANPQARVVVLAGAGKAFCAGHDLKQMQAH
PDQAYYEDLFARCTQMMLTIQRLPQPVIARVHGIATAAGCQLVAMCDLAVASTEARFAVSGITVGLFCSTPSVALSRNIG
RKAAFEMLVTGEFIDAVTAQRLGLVNRVAAPADLDAEVQRLVDAILAKPAESIAIGKSLFYRQLELPMEEAYRLAGQTMA
CNMMEPCAQEGVSAFVEKRPPAWANVER

Sequences:

>Translated_268_residues
MEGHEVAVEPFVLSEIDGRGVVRLTLNRPRQFNALSEEMLEALQRELDAIAANPQARVVVLAGAGKAFCAGHDLKQMQAH
PDQAYYEDLFARCTQMMLTIQRLPQPVIARVHGIATAAGCQLVAMCDLAVASTEARFAVSGITVGLFCSTPSVALSRNIG
RKAAFEMLVTGEFIDAVTAQRLGLVNRVAAPADLDAEVQRLVDAILAKPAESIAIGKSLFYRQLELPMEEAYRLAGQTMA
CNMMEPCAQEGVSAFVEKRPPAWANVER
>Mature_268_residues
MEGHEVAVEPFVLSEIDGRGVVRLTLNRPRQFNALSEEMLEALQRELDAIAANPQARVVVLAGAGKAFCAGHDLKQMQAH
PDQAYYEDLFARCTQMMLTIQRLPQPVIARVHGIATAAGCQLVAMCDLAVASTEARFAVSGITVGLFCSTPSVALSRNIG
RKAAFEMLVTGEFIDAVTAQRLGLVNRVAAPADLDAEVQRLVDAILAKPAESIAIGKSLFYRQLELPMEEAYRLAGQTMA
CNMMEPCAQEGVSAFVEKRPPAWANVER

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=246, Percent_Identity=41.869918699187, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI194097323, Length=252, Percent_Identity=30.5555555555556, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI70995211, Length=182, Percent_Identity=32.4175824175824, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI4502327, Length=255, Percent_Identity=25.0980392156863, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI37594469, Length=150, Percent_Identity=30.6666666666667, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI37594471, Length=150, Percent_Identity=30.6666666666667, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI4757966, Length=194, Percent_Identity=27.319587628866, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI51538581, Length=194, Percent_Identity=27.319587628866, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4757968, Length=236, Percent_Identity=25.4237288135593, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI49355787, Length=236, Percent_Identity=25.4237288135593, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI25453481, Length=194, Percent_Identity=27.319587628866, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI51538573, Length=194, Percent_Identity=27.319587628866, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1787659, Length=249, Percent_Identity=32.9317269076305, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1788597, Length=263, Percent_Identity=32.319391634981, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI221142681, Length=255, Percent_Identity=32.9411764705882, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1787660, Length=270, Percent_Identity=29.6296296296296, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87082183, Length=247, Percent_Identity=26.3157894736842, Blast_Score=84, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=246, Percent_Identity=41.0569105691057, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=29.2, Blast_Score=104, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17554946, Length=246, Percent_Identity=26.0162601626016, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17560910, Length=228, Percent_Identity=28.9473684210526, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17540714, Length=188, Percent_Identity=31.9148936170213, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=183, Percent_Identity=26.2295081967213, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=226, Percent_Identity=27.8761061946903, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=182, Percent_Identity=27.4725274725275, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=197, Percent_Identity=28.4263959390863, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=189, Percent_Identity=26.4550264550265, Blast_Score=72, Evalue=8e-14,
Organism=Drosophila melanogaster, GI19922422, Length=247, Percent_Identity=39.2712550607287, Blast_Score=185, Evalue=2e-47,
Organism=Drosophila melanogaster, GI20129971, Length=258, Percent_Identity=29.0697674418605, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24653477, Length=258, Percent_Identity=29.0697674418605, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24653139, Length=273, Percent_Identity=27.4725274725275, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24650670, Length=285, Percent_Identity=26.6666666666667, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI19920382, Length=186, Percent_Identity=29.5698924731183, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21357171, Length=192, Percent_Identity=27.6041666666667, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24654903, Length=196, Percent_Identity=26.0204081632653, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 29042; Mature: 29042

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGHEVAVEPFVLSEIDGRGVVRLTLNRPRQFNALSEEMLEALQRELDAIAANPQARVVV
CCCCCEEECCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
LAGAGKAFCAGHDLKQMQAHPDQAYYEDLFARCTQMMLTIQRLPQPVIARVHGIATAAGC
EECCCCHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
QLVAMCDLAVASTEARFAVSGITVGLFCSTPSVALSRNIGRKAAFEMLVTGEFIDAVTAQ
HHHHHHHHHHHCCCCCEEEECEEEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHH
RLGLVNRVAAPADLDAEVQRLVDAILAKPAESIAIGKSLFYRQLELPMEEAYRLAGQTMA
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHC
CNMMEPCAQEGVSAFVEKRPPAWANVER
CHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MEGHEVAVEPFVLSEIDGRGVVRLTLNRPRQFNALSEEMLEALQRELDAIAANPQARVVV
CCCCCEEECCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
LAGAGKAFCAGHDLKQMQAHPDQAYYEDLFARCTQMMLTIQRLPQPVIARVHGIATAAGC
EECCCCHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
QLVAMCDLAVASTEARFAVSGITVGLFCSTPSVALSRNIGRKAAFEMLVTGEFIDAVTAQ
HHHHHHHHHHHCCCCCEEEECEEEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHH
RLGLVNRVAAPADLDAEVQRLVDAILAKPAESIAIGKSLFYRQLELPMEEAYRLAGQTMA
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHC
CNMMEPCAQEGVSAFVEKRPPAWANVER
CHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA