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The map label for this gene is pdhC [H]

Identifier: 222524728

GI number: 222524728

Start: 1840553

End: 1841938

Strand: Reverse

Name: pdhC [H]

Synonym: Chy400_1455

Alternate gene names: 222524728

Gene position: 1841938-1840553 (Counterclockwise)

Preceding gene: 222524729

Following gene: 222524727

Centisome position: 34.96

GC content: 47.19

Gene sequence:

>1386_bases
ATGGTGCGTGAAGTAATTATGCCAAAATTTGGCTTTACCCAGGAGACTGCAGAAATTGTTCGCTGGATTAAACGTGAGGG
TGATTTGGTTGATATTGGCGAACCGATTGTTGAAGTAACTACTGATAAGGTCAATATGGAGGTTGAAGCACCTGCACGTG
GTGTACTTGGTAATGTGCGCTATCGAGAAGGTGACGTCGTACCGGTAACGGAAGTTATCGCGTACATTATGCCACTCGAA
GAAGTAGGGGTGCAATCCGCAAGTCAGAGCGGCACAGACCATCATATGGTTGTTAACTCTGCACCTTCACAACGCAGCGA
GCCGAAGGCAACACCGCTTGCTGAACGGGTAGCTCGCACAGTTGGATTGTCTCTTGACCGAATCCAGGGTAGTGGTCCAA
ACGGACGAATCCTTCGTCGTGACGTAGAAAACACTCTACGGCAGTTATCAATGAATAAAGTTCGAGCGGTGCCGGCAGCA
CGCCGATTAGCACGTGAAGCAGGTATTGATATAAGCAAAGTGAAAGGTACGGGTCCTCATCATCGTGTGCAATCCAGTGA
TGTGCAAAATTATTTGGCTACCAGATCTTCATTAGATGCGACATTTACCACGTCTGGGCCAATCAAGCAACCCGAAGATG
TTGTGACGTCTTCAACCATCACTGGTGATACCCAAACGCAATCCGGTTATCGCAAGCTGCCGCTCACAGGCATGCGTCGT
ACCATTGCTCGCCGACTCCAGCGGAGTGCACAGGAAGCTCCACATATCCAGCTTGAGGTACGCGTTGATGTAACTGAGGT
AGAAGCACTGCGAGCTAAAGCTAATCGACACCATCTTGACGAACAGCCTAAAGTTAGCTTGACAGCTATTTTTGTGAAGA
GTGCTGCATGGGCATTGAAACGGCACCCTTACCTAAATGCCTGGTTACAATCAATTGGGCATGAGGAACAGATCGTCTTA
GTTGATGAAATTAATATTGGTATTGCAGTTGCGCTTGAACAAGGGTTGATTGTACCAGTAATCCGAAAGGCAAATGAAAA
GGGGATATTACAAATAGCTCGAGAGATCGACGACCTGAGTCAGCGGGCTCGTACTGATAAACTACGTCCAGATGAGGTCG
TAGATGGTACTTTCTCTATCTCTAACCTTGGTATGTTTGGTATAGAGCGCTTTTCGGCAATTATCAATCCTCCACAGGTT
GCTATTTTGGCGGTTGGTGCAGTATGTCGAGAGGTTGTTGCTGATGAAAATAATGGAATTTCAGTTCGTCCCCTTGTGAA
GCTTACACTTTGTGTCGATCACAGAGTTGTCGATGGGGCGGTGGCTGCTGCTTTTCTTCACGATCTCAAGGTAGTGCTTG
AGGATCCACAGGTTATGTTGTTATAA

Upstream 100 bases:

>100_bases
ATCCCATATAACCGAACTCTTGAATATCACGCAGTGCCTCAGATTGAAAATATTATTGCGGCTGCTCGTGATTTAGTAGC
ATTAAGAGTGTGAGGAAACA

Downstream 100 bases:

>100_bases
AATGGGAGGTGATATGCAGCCAATAGATATTACTGCAAGTACTATCATTCTTGGGTTGGCCGCTGCCTGGATTTTGCAAT
TGTTCCTTTCCTATTTTCAA

Product: hypothetical protein

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MVREVIMPKFGFTQETAEIVRWIKREGDLVDIGEPIVEVTTDKVNMEVEAPARGVLGNVRYREGDVVPVTEVIAYIMPLE
EVGVQSASQSGTDHHMVVNSAPSQRSEPKATPLAERVARTVGLSLDRIQGSGPNGRILRRDVENTLRQLSMNKVRAVPAA
RRLAREAGIDISKVKGTGPHHRVQSSDVQNYLATRSSLDATFTTSGPIKQPEDVVTSSTITGDTQTQSGYRKLPLTGMRR
TIARRLQRSAQEAPHIQLEVRVDVTEVEALRAKANRHHLDEQPKVSLTAIFVKSAAWALKRHPYLNAWLQSIGHEEQIVL
VDEINIGIAVALEQGLIVPVIRKANEKGILQIAREIDDLSQRARTDKLRPDEVVDGTFSISNLGMFGIERFSAIINPPQV
AILAVGAVCREVVADENNGISVRPLVKLTLCVDHRVVDGAVAAAFLHDLKVVLEDPQVMLL

Sequences:

>Translated_461_residues
MVREVIMPKFGFTQETAEIVRWIKREGDLVDIGEPIVEVTTDKVNMEVEAPARGVLGNVRYREGDVVPVTEVIAYIMPLE
EVGVQSASQSGTDHHMVVNSAPSQRSEPKATPLAERVARTVGLSLDRIQGSGPNGRILRRDVENTLRQLSMNKVRAVPAA
RRLAREAGIDISKVKGTGPHHRVQSSDVQNYLATRSSLDATFTTSGPIKQPEDVVTSSTITGDTQTQSGYRKLPLTGMRR
TIARRLQRSAQEAPHIQLEVRVDVTEVEALRAKANRHHLDEQPKVSLTAIFVKSAAWALKRHPYLNAWLQSIGHEEQIVL
VDEINIGIAVALEQGLIVPVIRKANEKGILQIAREIDDLSQRARTDKLRPDEVVDGTFSISNLGMFGIERFSAIINPPQV
AILAVGAVCREVVADENNGISVRPLVKLTLCVDHRVVDGAVAAAFLHDLKVVLEDPQVMLL
>Mature_461_residues
MVREVIMPKFGFTQETAEIVRWIKREGDLVDIGEPIVEVTTDKVNMEVEAPARGVLGNVRYREGDVVPVTEVIAYIMPLE
EVGVQSASQSGTDHHMVVNSAPSQRSEPKATPLAERVARTVGLSLDRIQGSGPNGRILRRDVENTLRQLSMNKVRAVPAA
RRLAREAGIDISKVKGTGPHHRVQSSDVQNYLATRSSLDATFTTSGPIKQPEDVVTSSTITGDTQTQSGYRKLPLTGMRR
TIARRLQRSAQEAPHIQLEVRVDVTEVEALRAKANRHHLDEQPKVSLTAIFVKSAAWALKRHPYLNAWLQSIGHEEQIVL
VDEINIGIAVALEQGLIVPVIRKANEKGILQIAREIDDLSQRARTDKLRPDEVVDGTFSISNLGMFGIERFSAIINPPQV
AILAVGAVCREVVADENNGISVRPLVKLTLCVDHRVVDGAVAAAFLHDLKVVLEDPQVMLL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=476, Percent_Identity=31.3025210084034, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI203098816, Length=476, Percent_Identity=30.0420168067227, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI203098753, Length=476, Percent_Identity=30.0420168067227, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI110671329, Length=451, Percent_Identity=26.6075388026608, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI19923748, Length=225, Percent_Identity=36, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI260898739, Length=137, Percent_Identity=47.4452554744526, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1786305, Length=476, Percent_Identity=28.5714285714286, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1786946, Length=466, Percent_Identity=27.4678111587983, Blast_Score=153, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17560088, Length=469, Percent_Identity=34.5415778251599, Blast_Score=222, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI17538894, Length=326, Percent_Identity=32.2085889570552, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17537937, Length=449, Percent_Identity=26.7260579064588, Blast_Score=144, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI25146366, Length=225, Percent_Identity=34.6666666666667, Blast_Score=132, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6324258, Length=468, Percent_Identity=32.2649572649573, Blast_Score=200, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6320352, Length=258, Percent_Identity=34.4961240310078, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI20129315, Length=312, Percent_Identity=33.6538461538462, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24582497, Length=312, Percent_Identity=33.6538461538462, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI18859875, Length=319, Percent_Identity=30.7210031347962, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24645909, Length=232, Percent_Identity=31.4655172413793, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 50689; Mature: 50689

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL ; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVREVIMPKFGFTQETAEIVRWIKREGDLVDIGEPIVEVTTDKVNMEVEAPARGVLGNVR
CCCHHHCCCCCCCHHHHHHHHHHHHCCCEEECCCCCEEEECCCEEEEECCCCCCCCCCCE
YREGDVVPVTEVIAYIMPLEEVGVQSASQSGTDHHMVVNSAPSQRSEPKATPLAERVART
ECCCCCCCHHHHHHHHHCHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHH
VGLSLDRIQGSGPNGRILRRDVENTLRQLSMNKVRAVPAARRLAREAGIDISKVKGTGPH
HCCCHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCC
HRVQSSDVQNYLATRSSLDATFTTSGPIKQPEDVVTSSTITGDTQTQSGYRKLPLTGMRR
CCCCHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHCCCEECCCCCCCCCCCCCCCHHHHH
TIARRLQRSAQEAPHIQLEVRVDVTEVEALRAKANRHHLDEQPKVSLTAIFVKSAAWALK
HHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHH
RHPYLNAWLQSIGHEEQIVLVDEINIGIAVALEQGLIVPVIRKANEKGILQIAREIDDLS
CCCHHHHHHHHCCCCCEEEEEEECCCCEEEEECCCCEEEHHHCCCCCHHHHHHHHHHHHH
QRARTDKLRPDEVVDGTFSISNLGMFGIERFSAIINPPQVAILAVGAVCREVVADENNGI
HHHHHCCCCCCHHCCCCEEHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCE
SVRPLVKLTLCVDHRVVDGAVAAAFLHDLKVVLEDPQVMLL
EEEHHEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEC
>Mature Secondary Structure
MVREVIMPKFGFTQETAEIVRWIKREGDLVDIGEPIVEVTTDKVNMEVEAPARGVLGNVR
CCCHHHCCCCCCCHHHHHHHHHHHHCCCEEECCCCCEEEECCCEEEEECCCCCCCCCCCE
YREGDVVPVTEVIAYIMPLEEVGVQSASQSGTDHHMVVNSAPSQRSEPKATPLAERVART
ECCCCCCCHHHHHHHHHCHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHH
VGLSLDRIQGSGPNGRILRRDVENTLRQLSMNKVRAVPAARRLAREAGIDISKVKGTGPH
HCCCHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCC
HRVQSSDVQNYLATRSSLDATFTTSGPIKQPEDVVTSSTITGDTQTQSGYRKLPLTGMRR
CCCCHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHCCCEECCCCCCCCCCCCCCCHHHHH
TIARRLQRSAQEAPHIQLEVRVDVTEVEALRAKANRHHLDEQPKVSLTAIFVKSAAWALK
HHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHH
RHPYLNAWLQSIGHEEQIVLVDEINIGIAVALEQGLIVPVIRKANEKGILQIAREIDDLS
CCCHHHHHHHHCCCCCEEEEEEECCCCEEEEECCCCEEEHHHCCCCCHHHHHHHHHHHHH
QRARTDKLRPDEVVDGTFSISNLGMFGIERFSAIINPPQVAILAVGAVCREVVADENNGI
HHHHHCCCCCCHHCCCCEEHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCE
SVRPLVKLTLCVDHRVVDGAVAAAFLHDLKVVLEDPQVMLL
EEEHHEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]