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The map label for this gene is ptsH [H]

Identifier: 222524721

GI number: 222524721

Start: 1836224

End: 1836523

Strand: Reverse

Name: ptsH [H]

Synonym: Chy400_1448

Alternate gene names: 222524721

Gene position: 1836523-1836224 (Counterclockwise)

Preceding gene: 222524722

Following gene: 222524720

Centisome position: 34.86

GC content: 46.0

Gene sequence:

>300_bases
ATGAACACACAAGTCTCAACAATCATTACTATTGATCATCCAGCAGGTCTGCATGCTCGTCCTGCAGCGCGTTTCGTGAC
ACTGGCAGCTAGATTTCCCTGTTCCATTACGGTTCGTAAGTTGAATGGTTCGAAGCCACCAGTCAATGCTAAAAGTGCGC
TAGGTGTACTAACGCTAGCAGTTAATCAAGGCGATACAGTAGAGATCGTAGCTCAGGGCGATCAAGCTCAAGAAGCGATT
AGCGCTTTAATCGCTCTTATTCGGCATAACTTTGAGGAATCGACAACTTCTCATTCCTGA

Upstream 100 bases:

>100_bases
AAGCACATTGTCCAATTCACTATGATGAGGTTGAATTGAACAAAAGCCAGGTTATTTTTCAGCTTCGTCAGGAACAAGAT
CAATTATTGGGGTGTTAGCT

Downstream 100 bases:

>100_bases
TCAGGGATCTTTCTGAGTAGAGGATCTGGAAAAGATGCCACGATATCAAGGTCAATCAGGTTCACCTGGGATAGCTATCG
GTCCAGTATTCGTTTACCAT

Product: phosphotransferase system, phosphocarrier protein HPr

Products: D-sorbitol 6-phosphate [Cytoplasm]; pyruvate; alpha,alpha-trehalose 6-phosphate [Cytoplasm]; mannitol-1-phosphate [Cytoplasm]; D-glucosamine-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; galactitol-1-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm]; fructose-1-phosphate [Cytoplasm]; glucose-6-phosphate [Cytoplasm]; mannitol-1-phosphate [Cytoplasm]; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm]; salicin-6-phosphate [Cytoplasm]; arbutin-6-phosphate [Cytoplasm] [C]

Alternate protein names: Histidine-containing protein [H]

Number of amino acids: Translated: 99; Mature: 99

Protein sequence:

>99_residues
MNTQVSTIITIDHPAGLHARPAARFVTLAARFPCSITVRKLNGSKPPVNAKSALGVLTLAVNQGDTVEIVAQGDQAQEAI
SALIALIRHNFEESTTSHS

Sequences:

>Translated_99_residues
MNTQVSTIITIDHPAGLHARPAARFVTLAARFPCSITVRKLNGSKPPVNAKSALGVLTLAVNQGDTVEIVAQGDQAQEAI
SALIALIRHNFEESTTSHS
>Mature_99_residues
MNTQVSTIITIDHPAGLHARPAARFVTLAARFPCSITVRKLNGSKPPVNAKSALGVLTLAVNQGDTVEIVAQGDQAQEAI
SALIALIRHNFEESTTSHS

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1925

COG function: function code G; Phosphotransferase system, HPr-related proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HPr domain [H]

Homologues:

None

Paralogues:

None

Copy number: 4180 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 2100 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 3235 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114 [H]

Pfam domain/function: PF00381 PTS-HPr [H]

EC number: NA

Molecular weight: Translated: 10457; Mature: 10457

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: PS00369 PTS_HPR_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTQVSTIITIDHPAGLHARPAARFVTLAARFPCSITVRKLNGSKPPVNAKSALGVLTLA
CCCCEEEEEEECCCCCCCCCCHHHEEEHEECCCCEEEEEECCCCCCCCCCCCCCEEEEEE
VNQGDTVEIVAQGDQAQEAISALIALIRHNFEESTTSHS
ECCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNTQVSTIITIDHPAGLHARPAARFVTLAARFPCSITVRKLNGSKPPVNAKSALGVLTLA
CCCCEEEEEEECCCCCCCCCCHHHEEEHEECCCCEEEEEECCCCCCCCCCCCCCEEEEEE
VNQGDTVEIVAQGDQAQEAISALIALIRHNFEESTTSHS
ECCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sorbitol [Periplasm]; phosphoenolpyruvate; trehalose [Periplasm]; mannitol [Periplasm]; glucosamine [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; galactitol [Periplasm]; fructose [Periplasm]; beta-D-glucose [Periplasm]; diacetylchitobiose [Periplasm]; cellobiose [Periplasm]; salicin [Periplasm]; arbutin [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + sorbitol [Periplasm] = D-sorbitol 6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + trehalose [Periplasm] = alpha,alpha-trehalose 6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + mannitol [Periplasm] = mannitol-1-phosp

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]