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The map label for this gene is hisS [H]

Identifier: 222524658

GI number: 222524658

Start: 1764322

End: 1765773

Strand: Reverse

Name: hisS [H]

Synonym: Chy400_1385

Alternate gene names: 222524658

Gene position: 1765773-1764322 (Counterclockwise)

Preceding gene: 222524659

Following gene: 222524657

Centisome position: 33.51

GC content: 58.47

Gene sequence:

>1452_bases
ATGGGAGGCGCTGTGAGTATTGATCTGGTACGCGGCATGCGTGATGTCCATCCTGCGGAATTCGCGCATGGCCGATACAT
ACAACGTATTCTCGAACAGACGATGGCCGGTTACGGCTATCAGATGATTGATACGCCTGTCATCGAACATCGCGAACTCT
ACCTGCGTAAACTCGGTGAAGACCTGGTCGGTAAGGTCTACGAGTTTAGTTTTGGCGGTCGTGATCTGGCATTACGCCCG
GAATGGACGGCGTCGGTATTACGGGCTTACGTGGCTAGCATGCAGGATCAGCCATTGCCGTTACGCCTGAGCTATGCCGG
GCCGGTATTCCGCTATGAACGCCCCCAACGGTATACCTATCGCCAGTTTACCCAGGTCGGTGGTGAGATCATTGGTGGCC
TACCGCCACGTGCCGACGCTGAAGTTATTGCGCTTGCCTGTGCCGGTTTGCAGGCCGTAGGTGTAACCGAATACACGGTT
CGGATCGGGCATATTGGGCTGGCCCGCGAGTTGTTGAGTCGGTTTGCACTGCCGGAACGTACCCGTGGCCTGCTGGTCTG
GAGTCTCGAACGGTTGCGAGCTGAGGGAATTGCCCCGGTGCGTGAACGGGTGTTGGCCAATCTCGGTACCCCTCCAGAGG
GACTGGAACTTCCTGCCGGTCTGGATGATGAGCAGGCCGCACGCTGGCTTGAACGGGTGCTGACCGCGATGCAGATCGAT
CTACGCACGGGAACGCGCAGTCCGGCTGAAGTGCTTCAGCGCCTGCTCCGTACATTACGGCGCGCCGATGAACAGCCCCT
GGTCGAAGCGGCACTTGAGCAATTAGCCCGGCTGGCTGCCATCAGTGGCCCACCTGAACACGCTATCCCCATGCTGGCCG
ATCTGCCCGGCAGCGAGTCGGCAGCGTTTCGCGAACTACAGGCAATTCTCAATCTCCTCGCCGCTTACCATATTCCGGCT
GAGAGAGTTGTGATTGATGGTGCGCTGGGGCGCGGGTTACACTACTATACGGGCTTGATCTTTGAGATTTACGACCGCGA
GGGGAATCAGCTCTGTGGCGGTGGACGGTATGATGATCTGGTAGGCGCACTCGGTGGACGGCAGGCCACACCGGCGGTTG
GTTTTGCCTATGGGCTTGAACGGGTAACCGCAGCCGCAACGACCGGCAGCGGCGAGTCACCGACAACAGTGCTGGTTGCG
GCGGTGAGTGATGATGACTACCCCTATGCCTTGCATGTTGCCGAGCGGTTACGTGCCGGTGGTTATATTGTTGTACTGGA
TGTGCGTGGTCGCAGTGTTAAAGACAATCTCCGGGATGCGACACGACGCAATTACAGTGCGGTGGTGATTGTCGGTGCGG
CAGAACGGGCAGGTGAGTATGTGATTTGGCGCGATCTGGCGACACGTAGCGAGCGTCAGATACCCATCGCGATGTTGGGG
AGGCATCTGTGA

Upstream 100 bases:

>100_bases
CACAGCAATCTGTGCTCTGTTTTCGTCTACCGTATCAACCAGAGCGGTGTTGCACGCAACGTCTCTGCGTCGGTTACAAT
GGAATAAGGAGCAGCAACTG

Downstream 100 bases:

>100_bases
GGGTGGGTCTTACCTGGGGGCGTGCTGTTGAACTATTGCCTCATCAGCGCCAGCACATCCTCGCTCACTATCCGGTCTGC
TGGTTACCGTGGGCCATGGA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MGGAVSIDLVRGMRDVHPAEFAHGRYIQRILEQTMAGYGYQMIDTPVIEHRELYLRKLGEDLVGKVYEFSFGGRDLALRP
EWTASVLRAYVASMQDQPLPLRLSYAGPVFRYERPQRYTYRQFTQVGGEIIGGLPPRADAEVIALACAGLQAVGVTEYTV
RIGHIGLARELLSRFALPERTRGLLVWSLERLRAEGIAPVRERVLANLGTPPEGLELPAGLDDEQAARWLERVLTAMQID
LRTGTRSPAEVLQRLLRTLRRADEQPLVEAALEQLARLAAISGPPEHAIPMLADLPGSESAAFRELQAILNLLAAYHIPA
ERVVIDGALGRGLHYYTGLIFEIYDREGNQLCGGGRYDDLVGALGGRQATPAVGFAYGLERVTAAATTGSGESPTTVLVA
AVSDDDYPYALHVAERLRAGGYIVVLDVRGRSVKDNLRDATRRNYSAVVIVGAAERAGEYVIWRDLATRSERQIPIAMLG
RHL

Sequences:

>Translated_483_residues
MGGAVSIDLVRGMRDVHPAEFAHGRYIQRILEQTMAGYGYQMIDTPVIEHRELYLRKLGEDLVGKVYEFSFGGRDLALRP
EWTASVLRAYVASMQDQPLPLRLSYAGPVFRYERPQRYTYRQFTQVGGEIIGGLPPRADAEVIALACAGLQAVGVTEYTV
RIGHIGLARELLSRFALPERTRGLLVWSLERLRAEGIAPVRERVLANLGTPPEGLELPAGLDDEQAARWLERVLTAMQID
LRTGTRSPAEVLQRLLRTLRRADEQPLVEAALEQLARLAAISGPPEHAIPMLADLPGSESAAFRELQAILNLLAAYHIPA
ERVVIDGALGRGLHYYTGLIFEIYDREGNQLCGGGRYDDLVGALGGRQATPAVGFAYGLERVTAAATTGSGESPTTVLVA
AVSDDDYPYALHVAERLRAGGYIVVLDVRGRSVKDNLRDATRRNYSAVVIVGAAERAGEYVIWRDLATRSERQIPIAMLG
RHL
>Mature_482_residues
GGAVSIDLVRGMRDVHPAEFAHGRYIQRILEQTMAGYGYQMIDTPVIEHRELYLRKLGEDLVGKVYEFSFGGRDLALRPE
WTASVLRAYVASMQDQPLPLRLSYAGPVFRYERPQRYTYRQFTQVGGEIIGGLPPRADAEVIALACAGLQAVGVTEYTVR
IGHIGLARELLSRFALPERTRGLLVWSLERLRAEGIAPVRERVLANLGTPPEGLELPAGLDDEQAARWLERVLTAMQIDL
RTGTRSPAEVLQRLLRTLRRADEQPLVEAALEQLARLAAISGPPEHAIPMLADLPGSESAAFRELQAILNLLAAYHIPAE
RVVIDGALGRGLHYYTGLIFEIYDREGNQLCGGGRYDDLVGALGGRQATPAVGFAYGLERVTAAATTGSGESPTTVLVAA
VSDDDYPYALHVAERLRAGGYIVVLDVRGRSVKDNLRDATRRNYSAVVIVGAAERAGEYVIWRDLATRSERQIPIAMLGR
HL

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788861, Length=489, Percent_Identity=31.2883435582822, Blast_Score=146, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 53085; Mature: 52953

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGAVSIDLVRGMRDVHPAEFAHGRYIQRILEQTMAGYGYQMIDTPVIEHRELYLRKLGE
CCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEHHCCCHHHHHHHHHHHHCH
DLVGKVYEFSFGGRDLALRPEWTASVLRAYVASMQDQPLPLRLSYAGPVFRYERPQRYTY
HHHHHHHEECCCCCCEEECCHHHHHHHHHHHHHCCCCCCCEEEECCCCEEECCCCCCHHH
RQFTQVGGEIIGGLPPRADAEVIALACAGLQAVGVTEYTVRIGHIGLARELLSRFALPER
HHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHCCCEEEEEEECCHHHHHHHHHHHCCCCH
TRGLLVWSLERLRAEGIAPVRERVLANLGTPPEGLELPAGLDDEQAARWLERVLTAMQID
HCCCCEEEHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LRTGTRSPAEVLQRLLRTLRRADEQPLVEAALEQLARLAAISGPPEHAIPMLADLPGSES
HCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCH
AAFRELQAILNLLAAYHIPAERVVIDGALGRGLHYYTGLIFEIYDREGNQLCGGGRYDDL
HHHHHHHHHHHHHHHHCCCHHHEEEECHHCCCHHHHHHEEEEEEECCCCCCCCCCCHHHH
VGALGGRQATPAVGFAYGLERVTAAATTGSGESPTTVLVAAVSDDDYPYALHVAERLRAG
HHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCC
GYIVVLDVRGRSVKDNLRDATRRNYSAVVIVGAAERAGEYVIWRDLATRSERQIPIAMLG
CEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECHHHCCCEEEEECHHCCCCCCCCHHHHC
RHL
CCC
>Mature Secondary Structure 
GGAVSIDLVRGMRDVHPAEFAHGRYIQRILEQTMAGYGYQMIDTPVIEHRELYLRKLGE
CCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEHHCCCHHHHHHHHHHHHCH
DLVGKVYEFSFGGRDLALRPEWTASVLRAYVASMQDQPLPLRLSYAGPVFRYERPQRYTY
HHHHHHHEECCCCCCEEECCHHHHHHHHHHHHHCCCCCCCEEEECCCCEEECCCCCCHHH
RQFTQVGGEIIGGLPPRADAEVIALACAGLQAVGVTEYTVRIGHIGLARELLSRFALPER
HHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHCCCEEEEEEECCHHHHHHHHHHHCCCCH
TRGLLVWSLERLRAEGIAPVRERVLANLGTPPEGLELPAGLDDEQAARWLERVLTAMQID
HCCCCEEEHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LRTGTRSPAEVLQRLLRTLRRADEQPLVEAALEQLARLAAISGPPEHAIPMLADLPGSES
HCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCH
AAFRELQAILNLLAAYHIPAERVVIDGALGRGLHYYTGLIFEIYDREGNQLCGGGRYDDL
HHHHHHHHHHHHHHHHCCCHHHEEEECHHCCCHHHHHHEEEEEEECCCCCCCCCCCHHHH
VGALGGRQATPAVGFAYGLERVTAAATTGSGESPTTVLVAAVSDDDYPYALHVAERLRAG
HHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCC
GYIVVLDVRGRSVKDNLRDATRRNYSAVVIVGAAERAGEYVIWRDLATRSERQIPIAMLG
CEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECHHHCCCEEEEECHHCCCCCCCCHHHHC
RHL
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]