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The map label for this gene is greA

Identifier: 222524620

GI number: 222524620

Start: 1716868

End: 1717341

Strand: Reverse

Name: greA

Synonym: Chy400_1344

Alternate gene names: 222524620

Gene position: 1717341-1716868 (Counterclockwise)

Preceding gene: 222524626

Following gene: 222524619

Centisome position: 32.59

GC content: 54.01

Gene sequence:

>474_bases
ATGACAGAGAAGCCAACGTATCTGACTCGCGAAGGTCGCGCCCGTTTGGAGGCAGAGCTTGAGTATCTAACCACGGTTGA
GCGCAAGCAGATTGCCGAACGGATCGCAGCGGCCAAAGAATTGGGCGATATTTCCGAGAGCGGTGAATATGAGGATGCCA
AAAAGGCACAGGCGCTCCTTGAAGGTCGTATTCGCGAGCTGAAGCACTTGCTCTCACGCGCTGAAGTCATTGATGAGGAT
CAGGCCAGCAATGGTGAAGTACGGGTTGGTTCATCTGTAACCGTGCGCTTTGAAGACGATGGTACAGAAGAGACCTGGAC
GATTGTTGGTAGTGCGGAAGCCAATCCGCGCCAGGGCCGTATCTCAAATGAGTCACCGCTCGGTGCTGCACTGCTCGGCA
AGCGCGCACGTAATAAGGTGACCGTTCACACGCCTTCCGGCGTCATGAAATTAACCATTCTCAAAGTGCGTTAG

Upstream 100 bases:

>100_bases
CCTGACTTCAACTGAGAGATGTGCTCTCAGTTATTCTTTTTATATTGAGGGTTTTCCGACCGTCCGGTTGGGCGGTGCGG
GCAGCGTAGAGGCAGTAGTG

Downstream 100 bases:

>100_bases
TAATTAACGATTGTGGACGGGTGAACCACCTTTGTCAGCAACAACTATCTGGTAGTTGCTGATTGCCTGGTGCTGATTGT
TGTTTTGAGAGGGCGTCGGT

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MTEKPTYLTREGRARLEAELEYLTTVERKQIAERIAAAKELGDISESGEYEDAKKAQALLEGRIRELKHLLSRAEVIDED
QASNGEVRVGSSVTVRFEDDGTEETWTIVGSAEANPRQGRISNESPLGAALLGKRARNKVTVHTPSGVMKLTILKVR

Sequences:

>Translated_157_residues
MTEKPTYLTREGRARLEAELEYLTTVERKQIAERIAAAKELGDISESGEYEDAKKAQALLEGRIRELKHLLSRAEVIDED
QASNGEVRVGSSVTVRFEDDGTEETWTIVGSAEANPRQGRISNESPLGAALLGKRARNKVTVHTPSGVMKLTILKVR
>Mature_156_residues
TEKPTYLTREGRARLEAELEYLTTVERKQIAERIAAAKELGDISESGEYEDAKKAQALLEGRIRELKHLLSRAEVIDEDQ
ASNGEVRVGSSVTVRFEDDGTEETWTIVGSAEANPRQGRISNESPLGAALLGKRARNKVTVHTPSGVMKLTILKVR

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=150, Percent_Identity=39.3333333333333, Blast_Score=105, Evalue=1e-24,
Organism=Escherichia coli, GI87082261, Length=140, Percent_Identity=35, Blast_Score=83, Evalue=9e-18,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_CHLAA (A9WK05)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001634845.1
- ProteinModelPortal:   A9WK05
- SMR:   A9WK05
- GeneID:   5828331
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_1227
- HOGENOM:   HBG753387
- OMA:   NHEVRIG
- ProtClustDB:   PRK00226
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17361; Mature: 17230

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKPTYLTREGRARLEAELEYLTTVERKQIAERIAAAKELGDISESGEYEDAKKAQALL
CCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
EGRIRELKHLLSRAEVIDEDQASNGEVRVGSSVTVRFEDDGTEETWTIVGSAEANPRQGR
HHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEECCCCCCEEEEEEECCCCCCCCCC
ISNESPLGAALLGKRARNKVTVHTPSGVMKLTILKVR
CCCCCCCCHHHHHHHCCCEEEEECCCCEEEEEEEEEC
>Mature Secondary Structure 
TEKPTYLTREGRARLEAELEYLTTVERKQIAERIAAAKELGDISESGEYEDAKKAQALL
CCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
EGRIRELKHLLSRAEVIDEDQASNGEVRVGSSVTVRFEDDGTEETWTIVGSAEANPRQGR
HHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEECCCCCCEEEEEEECCCCCCCCCC
ISNESPLGAALLGKRARNKVTVHTPSGVMKLTILKVR
CCCCCCCCHHHHHHHCCCEEEEECCCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA