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The map label for this gene is lysS

Identifier: 222524619

GI number: 222524619

Start: 1715194

End: 1716669

Strand: Reverse

Name: lysS

Synonym: Chy400_1343

Alternate gene names: 222524619

Gene position: 1716669-1715194 (Counterclockwise)

Preceding gene: 222524620

Following gene: 222524618

Centisome position: 32.58

GC content: 55.28

Gene sequence:

>1476_bases
ATGGAATTGAATGATTTACAGGCCCAACGTGCGGCAAAGCTGGCCGAATTGCGTGCTGCCGGTCTCGATCCATACCCACC
TCGCTGTTACCGCAGCCATACCATTGCCGAAGCTCTGGCTGCGTTTGATGATCTGGTCGCCCAGTCCACCACACTGACGC
TGACCGGACGCATCATCGGCGCTCGCCGCATTATGGGTAAGATCGCCTTTGCTCATATTGAAGATGGCACGGGTGAGATT
CAGCTCTGGCTCAGCCGGGCCGATCTCGGTGATGAATGGTTCGAGCGTTTTCGCGATCAGCTCGACACCTTCGATATTGT
CCAGGCGAGTGGTGTATTACGTTGCACCAAGACCGGTGAACGCTCGCTCTTTGTGCGTGAGCTGGCAATACTGGCCAAGG
CGATTAATCCACCCCCTGAAAAATGGGCCGGTTTGCAAGATGTCGAAGAGCGCCATCGCCAGCGCTATCTCGACCTGATC
GTTAACCGTGACCGCCGCGAGATTTTCCGGGCGCGGGCCCGTGTCATCAGCACGATGCGGCGCGTCCTGGATGAACGGGG
CTTTCTCGAAGTTGAGACACCGGTGTTGCAACCACTCTACGGTGGGGCTGCTGCACGCCCTTTTATCACCTATCACAATG
CGCTGGGCCAGAATCTCTATTTGCGGATCGCCACCGAACTCTACCTCAAGCGCCTGATTGTCGGCGGCTTTCCCGGTGTG
TACGAAATCGGGAAGAACTTTCGCAATGAAGGGGTTGATCGCTCACACAATCCAGAGTTCACCATGATGGAATGTTATCA
GGCGTATGCTGATTATCACGCCATGATGACTCTGGTGGAAGAGATGCTGAGCGAGATTTGTCTGGCTGTCCACGGCACCA
CAACGATCACCTATCAGGGCCGTGAACTTGATTTTCGTCCACCCTGGCCGCGCATTGCGATGGCAACAGCGATTGCCGAC
CGCACCGGTATCGACATTACCCAGATCACCGATCTGGATGCCTTACAAGAAGCTATCAGCGCACGTGGATTGCGGGTTGA
ACGCAAGGCGTCGTGGGCAAAGCAGGTTGACGAACTCTTCTCGGAGTTTGTACAACCTCATCTGTTCCAGCCAACCTTCA
TCATCGATTATCCGGTGGCGATGTCGCCGCTGGCAAAGCGGATTCCTGACCGGCCTGATTTTACCGAGCGCTTCGAGGCC
TTTATCGCCGGTATGGAGATTGGCAATGCCTTCACCGAGTTGAATGATCCATTCGACCAGGAAGAACGCTTCCGTGAACA
GTTACGCGCCTTCGCTGCCGGTGATGAAGAGGCGCACCAGATGGACGAAGATTTTATCAATGCGCTACGTTACGGTATGC
CACCGACCGGTGGTCTTGGGGTAGGTATCGACCGCCTGGTGATGGTCTTGACCGATCAATCCAACATTCGCGAAGTGATT
CTCTTCCCGCATTTGCGGGAACGGTCTGATGAGTAG

Upstream 100 bases:

>100_bases
GTAAGCCGATGCCCTATTGTCTTTTTTCCGGTATAATGGGAAGAGCTGTGCCGGTGCTTTGAAGATATTCTCTGGCTTGA
TACTATCGTGAAAACATGCT

Downstream 100 bases:

>100_bases
CGAGCGGTGCAGGCAATCTATCGCCGGAAAGGAGGAGCGTATGCCCCGCTCATTTACGGTTGAGCGTGAGAGTTTGCCTG
CGGTTGTGCAACGCTGGATC

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MELNDLQAQRAAKLAELRAAGLDPYPPRCYRSHTIAEALAAFDDLVAQSTTLTLTGRIIGARRIMGKIAFAHIEDGTGEI
QLWLSRADLGDEWFERFRDQLDTFDIVQASGVLRCTKTGERSLFVRELAILAKAINPPPEKWAGLQDVEERHRQRYLDLI
VNRDRREIFRARARVISTMRRVLDERGFLEVETPVLQPLYGGAAARPFITYHNALGQNLYLRIATELYLKRLIVGGFPGV
YEIGKNFRNEGVDRSHNPEFTMMECYQAYADYHAMMTLVEEMLSEICLAVHGTTTITYQGRELDFRPPWPRIAMATAIAD
RTGIDITQITDLDALQEAISARGLRVERKASWAKQVDELFSEFVQPHLFQPTFIIDYPVAMSPLAKRIPDRPDFTERFEA
FIAGMEIGNAFTELNDPFDQEERFREQLRAFAAGDEEAHQMDEDFINALRYGMPPTGGLGVGIDRLVMVLTDQSNIREVI
LFPHLRERSDE

Sequences:

>Translated_491_residues
MELNDLQAQRAAKLAELRAAGLDPYPPRCYRSHTIAEALAAFDDLVAQSTTLTLTGRIIGARRIMGKIAFAHIEDGTGEI
QLWLSRADLGDEWFERFRDQLDTFDIVQASGVLRCTKTGERSLFVRELAILAKAINPPPEKWAGLQDVEERHRQRYLDLI
VNRDRREIFRARARVISTMRRVLDERGFLEVETPVLQPLYGGAAARPFITYHNALGQNLYLRIATELYLKRLIVGGFPGV
YEIGKNFRNEGVDRSHNPEFTMMECYQAYADYHAMMTLVEEMLSEICLAVHGTTTITYQGRELDFRPPWPRIAMATAIAD
RTGIDITQITDLDALQEAISARGLRVERKASWAKQVDELFSEFVQPHLFQPTFIIDYPVAMSPLAKRIPDRPDFTERFEA
FIAGMEIGNAFTELNDPFDQEERFREQLRAFAAGDEEAHQMDEDFINALRYGMPPTGGLGVGIDRLVMVLTDQSNIREVI
LFPHLRERSDE
>Mature_491_residues
MELNDLQAQRAAKLAELRAAGLDPYPPRCYRSHTIAEALAAFDDLVAQSTTLTLTGRIIGARRIMGKIAFAHIEDGTGEI
QLWLSRADLGDEWFERFRDQLDTFDIVQASGVLRCTKTGERSLFVRELAILAKAINPPPEKWAGLQDVEERHRQRYLDLI
VNRDRREIFRARARVISTMRRVLDERGFLEVETPVLQPLYGGAAARPFITYHNALGQNLYLRIATELYLKRLIVGGFPGV
YEIGKNFRNEGVDRSHNPEFTMMECYQAYADYHAMMTLVEEMLSEICLAVHGTTTITYQGRELDFRPPWPRIAMATAIAD
RTGIDITQITDLDALQEAISARGLRVERKASWAKQVDELFSEFVQPHLFQPTFIIDYPVAMSPLAKRIPDRPDFTERFEA
FIAGMEIGNAFTELNDPFDQEERFREQLRAFAAGDEEAHQMDEDFINALRYGMPPTGGLGVGIDRLVMVLTDQSNIREVI
LFPHLRERSDE

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI5031815, Length=495, Percent_Identity=41.010101010101, Blast_Score=373, Evalue=1e-103,
Organism=Homo sapiens, GI194272210, Length=501, Percent_Identity=40.7185628742515, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI45439306, Length=463, Percent_Identity=22.2462203023758, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1790571, Length=492, Percent_Identity=46.7479674796748, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI1789256, Length=492, Percent_Identity=46.7479674796748, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI87082379, Length=319, Percent_Identity=31.3479623824451, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1788173, Length=341, Percent_Identity=26.099706744868, Blast_Score=76, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=487, Percent_Identity=40.6570841889117, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17535927, Length=487, Percent_Identity=40.6570841889117, Blast_Score=364, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI71994340, Length=487, Percent_Identity=40.6570841889117, Blast_Score=363, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17551876, Length=336, Percent_Identity=25, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI32566633, Length=170, Percent_Identity=28.8235294117647, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320242, Length=502, Percent_Identity=41.6334661354582, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6324256, Length=478, Percent_Identity=31.3807531380753, Blast_Score=189, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6323011, Length=328, Percent_Identity=25.609756097561, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24640849, Length=503, Percent_Identity=39.9602385685885, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24640851, Length=503, Percent_Identity=39.9602385685885, Blast_Score=374, Evalue=1e-103,
Organism=Drosophila melanogaster, GI17136276, Length=458, Percent_Identity=22.2707423580786, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYK_CHLAA (A9WK04)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001634844.1
- ProteinModelPortal:   A9WK04
- SMR:   A9WK04
- GeneID:   5828330
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_1226
- HOGENOM:   HBG631383
- OMA:   RNEGMDH
- ProtClustDB:   PRK00484
- GO:   GO:0005737
- HAMAP:   MF_00252
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF4
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00499

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.6

Molecular weight: Translated: 56004; Mature: 56004

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELNDLQAQRAAKLAELRAAGLDPYPPRCYRSHTIAEALAAFDDLVAQSTTLTLTGRIIG
CCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHH
ARRIMGKIAFAHIEDGTGEIQLWLSRADLGDEWFERFRDQLDTFDIVQASGVLRCTKTGE
HHHHHHHHHHEEECCCCCEEEEEEECCCCCHHHHHHHHHHCCHHHHHHHCCCEEEECCCC
RSLFVRELAILAKAINPPPEKWAGLQDVEERHRQRYLDLIVNRDRREIFRARARVISTMR
HHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RVLDERGFLEVETPVLQPLYGGAAARPFITYHNALGQNLYLRIATELYLKRLIVGGFPGV
HHHHHCCCEEECCHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCH
YEIGKNFRNEGVDRSHNPEFTMMECYQAYADYHAMMTLVEEMLSEICLAVHGTTTITYQG
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
RELDFRPPWPRIAMATAIADRTGIDITQITDLDALQEAISARGLRVERKASWAKQVDELF
CCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
SEFVQPHLFQPTFIIDYPVAMSPLAKRIPDRPDFTERFEAFIAGMEIGNAFTELNDPFDQ
HHHHCCCCCCCEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCH
EERFREQLRAFAAGDEEAHQMDEDFINALRYGMPPTGGLGVGIDRLVMVLTDQSNIREVI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHEEEEECCCCCCEEE
LFPHLRERSDE
ECCCHHCCCCC
>Mature Secondary Structure
MELNDLQAQRAAKLAELRAAGLDPYPPRCYRSHTIAEALAAFDDLVAQSTTLTLTGRIIG
CCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHH
ARRIMGKIAFAHIEDGTGEIQLWLSRADLGDEWFERFRDQLDTFDIVQASGVLRCTKTGE
HHHHHHHHHHEEECCCCCEEEEEEECCCCCHHHHHHHHHHCCHHHHHHHCCCEEEECCCC
RSLFVRELAILAKAINPPPEKWAGLQDVEERHRQRYLDLIVNRDRREIFRARARVISTMR
HHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RVLDERGFLEVETPVLQPLYGGAAARPFITYHNALGQNLYLRIATELYLKRLIVGGFPGV
HHHHHCCCEEECCHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCH
YEIGKNFRNEGVDRSHNPEFTMMECYQAYADYHAMMTLVEEMLSEICLAVHGTTTITYQG
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
RELDFRPPWPRIAMATAIADRTGIDITQITDLDALQEAISARGLRVERKASWAKQVDELF
CCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
SEFVQPHLFQPTFIIDYPVAMSPLAKRIPDRPDFTERFEAFIAGMEIGNAFTELNDPFDQ
HHHHCCCCCCCEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCH
EERFREQLRAFAAGDEEAHQMDEDFINALRYGMPPTGGLGVGIDRLVMVLTDQSNIREVI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHEEEEECCCCCCEEE
LFPHLRERSDE
ECCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA