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The map label for this gene is thrS [H]

Identifier: 222524584

GI number: 222524584

Start: 1674351

End: 1676135

Strand: Reverse

Name: thrS [H]

Synonym: Chy400_1308

Alternate gene names: 222524584

Gene position: 1676135-1674351 (Counterclockwise)

Preceding gene: 222524585

Following gene: 222524583

Centisome position: 31.81

GC content: 57.87

Gene sequence:

>1785_bases
ATGCCAGTCGATCCCAACAGCGATCCCTATTACCGCCTGCGGCATTCGCTGGCCCACGTGATGGCCCAGGCTGTGCTCGA
AATCTTTCCCGATGCCAAAATTGCGATTGGGCCACCGATTGAAAACGGCTTTTACTACGACTTTGATCTGCCGCGCCCGC
TCACGCCCGAAGACTTGAGCGAGATCGAGCGACGGATGAAGCGGATTATTGCCGGCAATCACCCCTTCATCTACCGTGAG
GTGACGGCTGAAGAGGCTCGTCAGATCTTTGCCAATCAACCGTATAAACTGGAATTGATCGAAGGCCTGGCCAAAGGGCT
TGATGAATATGGCGAGACGCACCACGGCGATACCGTCATCTCAACCTACCGCCAGGATACGTTCGAGGACCTCTGCCGCG
GTCCGCATCTCAACCATACCGGCGAGATCAATCCCGAAGCCTTCAAGCTGATGAGCATCGCCGGCGCCTACTGGCGGGGT
GATGAGAAGAATCGGCAGTTGCAGCGTATCTACGGCACCGGCTGGAATACGAAAGAGGAGCTGGAAGCCTATCTGCACCG
GTTGGAAGAGGCGCGTAAGCGCGACCACCGCCGTCTGGGCAAAGAGCTGGGACTCTTCTTCTTTTCAGATGATATTGGTC
CCGGTCTGCCGATCTTCACCCCCAAAGGGGAAATCATTCGCCACGAAATGGAGAAGTTTGTGCGCGAAGTCCAGACCCGC
TACGGGTATCAACATGTCTGGACGGGCAATGTGGTGAAGGAACAGCTTTACCGCAAGTCGGGCCACTACGACAACTACCG
TGACAGCATGTTCCCGCCGATGGTCGAGAGCGAAGACCTTGTCTTCCGGCTGAAGCCGATGAACTGCCCCAGCCACATGA
CGCTCTACAACCAGCTTGGCGTGATCAGTTACCGCGACCTTCCGCTAAGGTTTGCCGAATTTGCGACGCTCTATCGGTAT
GAAGATAGTGGAGCGCTGAGCGGCTTAACGCGCGTGCGCGCCTTAACCCAAGACGACTGCCACATCTTCTGTCGGCCCGA
TCAGATACAAGAAGAGTTCAGCCTGGCGCTCCAGGTCATTCGCGAAGTGCTGGGCACCTATCAGTTTACCGATTACAAAG
TGCGGCTATCGTTGCGCGGCAGCGAAGGCAAATACGTTCAGGACGATGAGAAGTGGGAGCTGGCAACCGCCGCGCTGCGC
AGTGCACTGGAAGCCAACGGTGTCGAGTACTTTGAAGTCGAAGGTGAAGCGGCATTCTACGGCCCGAAAGCCGACTTCCT
TGCCCGCGATGTGCTGGGGCGTGAATGGCAACTGAGCACCATCCAGGTCGATTTTATCCAGCCGGCCCGTCTGGGGTGTG
AATATATCGGCGAAGACGACAAACCCCACACACCGGTGGTCTTGCACCGCGCCGTTACCGGCACGACCGAGCGGTTTATG
GGTATCCTGATCGAGCACTACGGCGGCGCGTTTCCGCTCTGGCTGGCGCCGGTGCAGGCGGTCGTCATCCCGATTACCGA
TAAACAGATGGAGTTTGCCCGCCAGGTGCGCCAGCGCCTGTTCATGGCGGGGCTGCGGGTCGAACTCGACGACAACCGCG
ACCGCATGCAGGGCAAGATTCGCCGTGCTCAGTTGCAGAAGATTCCGTATATGCTTATAATCGGCGCCAGGGAGGAAGCT
GCTGACTCTGTTGCAGTACGCACCCGCGCAGGTGAAGACCTTGGCGCAATGCCGGTTGATGCATTCCTGGCCCGTGCTCT
GGAGGAGATTCGCACCCGCAGTTGA

Upstream 100 bases:

>100_bases
TGAACTGAACCGTCAAAACCGCCAGATTGTTGATGCACGTATCACCGACGGCTACCTGAGCATTGTCACAACCAGTAGAT
AACAAGAAGGAGAACCCAGT

Downstream 100 bases:

>100_bases
GGAGTGTTCCAATGACGGTTGAGAACGATGCTATCGCCGAAGTGGCTCGCCGCTTTGCCGAGTCGATCCACGCTGAACGC
ACGGCAACCCTCGAAGCATT

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 594; Mature: 593

Protein sequence:

>594_residues
MPVDPNSDPYYRLRHSLAHVMAQAVLEIFPDAKIAIGPPIENGFYYDFDLPRPLTPEDLSEIERRMKRIIAGNHPFIYRE
VTAEEARQIFANQPYKLELIEGLAKGLDEYGETHHGDTVISTYRQDTFEDLCRGPHLNHTGEINPEAFKLMSIAGAYWRG
DEKNRQLQRIYGTGWNTKEELEAYLHRLEEARKRDHRRLGKELGLFFFSDDIGPGLPIFTPKGEIIRHEMEKFVREVQTR
YGYQHVWTGNVVKEQLYRKSGHYDNYRDSMFPPMVESEDLVFRLKPMNCPSHMTLYNQLGVISYRDLPLRFAEFATLYRY
EDSGALSGLTRVRALTQDDCHIFCRPDQIQEEFSLALQVIREVLGTYQFTDYKVRLSLRGSEGKYVQDDEKWELATAALR
SALEANGVEYFEVEGEAAFYGPKADFLARDVLGREWQLSTIQVDFIQPARLGCEYIGEDDKPHTPVVLHRAVTGTTERFM
GILIEHYGGAFPLWLAPVQAVVIPITDKQMEFARQVRQRLFMAGLRVELDDNRDRMQGKIRRAQLQKIPYMLIIGAREEA
ADSVAVRTRAGEDLGAMPVDAFLARALEEIRTRS

Sequences:

>Translated_594_residues
MPVDPNSDPYYRLRHSLAHVMAQAVLEIFPDAKIAIGPPIENGFYYDFDLPRPLTPEDLSEIERRMKRIIAGNHPFIYRE
VTAEEARQIFANQPYKLELIEGLAKGLDEYGETHHGDTVISTYRQDTFEDLCRGPHLNHTGEINPEAFKLMSIAGAYWRG
DEKNRQLQRIYGTGWNTKEELEAYLHRLEEARKRDHRRLGKELGLFFFSDDIGPGLPIFTPKGEIIRHEMEKFVREVQTR
YGYQHVWTGNVVKEQLYRKSGHYDNYRDSMFPPMVESEDLVFRLKPMNCPSHMTLYNQLGVISYRDLPLRFAEFATLYRY
EDSGALSGLTRVRALTQDDCHIFCRPDQIQEEFSLALQVIREVLGTYQFTDYKVRLSLRGSEGKYVQDDEKWELATAALR
SALEANGVEYFEVEGEAAFYGPKADFLARDVLGREWQLSTIQVDFIQPARLGCEYIGEDDKPHTPVVLHRAVTGTTERFM
GILIEHYGGAFPLWLAPVQAVVIPITDKQMEFARQVRQRLFMAGLRVELDDNRDRMQGKIRRAQLQKIPYMLIIGAREEA
ADSVAVRTRAGEDLGAMPVDAFLARALEEIRTRS
>Mature_593_residues
PVDPNSDPYYRLRHSLAHVMAQAVLEIFPDAKIAIGPPIENGFYYDFDLPRPLTPEDLSEIERRMKRIIAGNHPFIYREV
TAEEARQIFANQPYKLELIEGLAKGLDEYGETHHGDTVISTYRQDTFEDLCRGPHLNHTGEINPEAFKLMSIAGAYWRGD
EKNRQLQRIYGTGWNTKEELEAYLHRLEEARKRDHRRLGKELGLFFFSDDIGPGLPIFTPKGEIIRHEMEKFVREVQTRY
GYQHVWTGNVVKEQLYRKSGHYDNYRDSMFPPMVESEDLVFRLKPMNCPSHMTLYNQLGVISYRDLPLRFAEFATLYRYE
DSGALSGLTRVRALTQDDCHIFCRPDQIQEEFSLALQVIREVLGTYQFTDYKVRLSLRGSEGKYVQDDEKWELATAALRS
ALEANGVEYFEVEGEAAFYGPKADFLARDVLGREWQLSTIQVDFIQPARLGCEYIGEDDKPHTPVVLHRAVTGTTERFMG
ILIEHYGGAFPLWLAPVQAVVIPITDKQMEFARQVRQRLFMAGLRVELDDNRDRMQGKIRRAQLQKIPYMLIIGAREEAA
DSVAVRTRAGEDLGAMPVDAFLARALEEIRTRS

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=584, Percent_Identity=37.3287671232877, Blast_Score=379, Evalue=1e-105,
Organism=Homo sapiens, GI38570111, Length=583, Percent_Identity=36.7066895368782, Blast_Score=378, Evalue=1e-104,
Organism=Homo sapiens, GI20070344, Length=597, Percent_Identity=36.6834170854271, Blast_Score=360, Evalue=2e-99,
Organism=Escherichia coli, GI1788013, Length=584, Percent_Identity=44.5205479452055, Blast_Score=509, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI71984184, Length=574, Percent_Identity=37.8048780487805, Blast_Score=387, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71984192, Length=574, Percent_Identity=37.8048780487805, Blast_Score=387, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6322112, Length=569, Percent_Identity=36.7311072056239, Blast_Score=358, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6322654, Length=392, Percent_Identity=33.9285714285714, Blast_Score=229, Evalue=7e-61,
Organism=Drosophila melanogaster, GI24583842, Length=579, Percent_Identity=37.13298791019, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI28574141, Length=579, Percent_Identity=37.13298791019, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24583839, Length=579, Percent_Identity=37.13298791019, Blast_Score=380, Evalue=1e-105,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 68502; Mature: 68371

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVDPNSDPYYRLRHSLAHVMAQAVLEIFPDAKIAIGPPIENGFYYDFDLPRPLTPEDLS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEEECCCCCCCCHHHHH
EIERRMKRIIAGNHPFIYREVTAEEARQIFANQPYKLELIEGLAKGLDEYGETHHGDTVI
HHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCCCCCHHH
STYRQDTFEDLCRGPHLNHTGEINPEAFKLMSIAGAYWRGDEKNRQLQRIYGTGWNTKEE
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHH
LEAYLHRLEEARKRDHRRLGKELGLFFFSDDIGPGLPIFTPKGEIIRHEMEKFVREVQTR
HHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
YGYQHVWTGNVVKEQLYRKSGHYDNYRDSMFPPMVESEDLVFRLKPMNCPSHMTLYNQLG
CCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHCC
VISYRDLPLRFAEFATLYRYEDSGALSGLTRVRALTQDDCHIFCRPDQIQEEFSLALQVI
EEEECCCCHHHHHHHHHHEECCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHH
REVLGTYQFTDYKVRLSLRGSEGKYVQDDEKWELATAALRSALEANGVEYFEVEGEAAFY
HHHHHCCEECCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCEEEE
GPKADFLARDVLGREWQLSTIQVDFIQPARLGCEYIGEDDKPHTPVVLHRAVTGTTERFM
CCCHHHHHHHHCCCCEEEEEEEEEECCHHHHCHHHCCCCCCCCCCEEEEEHHHCCHHHHH
GILIEHYGGAFPLWLAPVQAVVIPITDKQMEFARQVRQRLFMAGLRVELDDNRDRMQGKI
HHHHHHCCCCCHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHH
RRAQLQKIPYMLIIGAREEAADSVAVRTRAGEDLGAMPVDAFLARALEEIRTRS
HHHHHHHCCEEEEEECCHHHHCHHEEEECCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PVDPNSDPYYRLRHSLAHVMAQAVLEIFPDAKIAIGPPIENGFYYDFDLPRPLTPEDLS
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEEEECCCCCCCCHHHHH
EIERRMKRIIAGNHPFIYREVTAEEARQIFANQPYKLELIEGLAKGLDEYGETHHGDTVI
HHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCCCCCHHH
STYRQDTFEDLCRGPHLNHTGEINPEAFKLMSIAGAYWRGDEKNRQLQRIYGTGWNTKEE
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHH
LEAYLHRLEEARKRDHRRLGKELGLFFFSDDIGPGLPIFTPKGEIIRHEMEKFVREVQTR
HHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
YGYQHVWTGNVVKEQLYRKSGHYDNYRDSMFPPMVESEDLVFRLKPMNCPSHMTLYNQLG
CCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHCC
VISYRDLPLRFAEFATLYRYEDSGALSGLTRVRALTQDDCHIFCRPDQIQEEFSLALQVI
EEEECCCCHHHHHHHHHHEECCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHH
REVLGTYQFTDYKVRLSLRGSEGKYVQDDEKWELATAALRSALEANGVEYFEVEGEAAFY
HHHHHCCEECCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCEEEE
GPKADFLARDVLGREWQLSTIQVDFIQPARLGCEYIGEDDKPHTPVVLHRAVTGTTERFM
CCCHHHHHHHHCCCCEEEEEEEEEECCHHHHCHHHCCCCCCCCCCEEEEEHHHCCHHHHH
GILIEHYGGAFPLWLAPVQAVVIPITDKQMEFARQVRQRLFMAGLRVELDDNRDRMQGKI
HHHHHHCCCCCHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHH
RRAQLQKIPYMLIIGAREEAADSVAVRTRAGEDLGAMPVDAFLARALEEIRTRS
HHHHHHHCCEEEEEECCHHHHCHHEEEECCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA