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The map label for this gene is oppA [H]

Identifier: 222524542

GI number: 222524542

Start: 1611803

End: 1613701

Strand: Reverse

Name: oppA [H]

Synonym: Chy400_1266

Alternate gene names: 222524542

Gene position: 1613701-1611803 (Counterclockwise)

Preceding gene: 222524543

Following gene: 222524541

Centisome position: 30.63

GC content: 54.4

Gene sequence:

>1899_bases
ATGAAACACCGCTTCAGTCTTTTGATTGTTTTCATGGTGAGCGTGATACTAACCGCTTGTGGCCCATCTCCTGCACCGGT
GGGGCAACCGACTACGGTGCAGCCAGCGCAGGCTCCAACTGCGCTGACCGAAGCGTCTGCACCGACACAGGTTGCAGCCC
CGATAAGCGCTGATGCATTGCCACGTAAGGAAACGCTCTATTTCAATGGCTTACAGTGGGGACCGGTCGTCGGTTGGAAT
CCCTATTCCAATAACAACAACAACGGCATGGTGATTGCGCTACAAGACAGCGCTCGCGTGACCATGTTCGAGACGCCGTA
TTTGTATAACATGTTAGACGGTAAGCAATATCCATTGCTGGCTGATGGCGACTGGACATGGAACGCAGATCGCACCGAAA
TCACCTTCAAGATCAAATCGGCTGCCCGCTGGAGCGACGGCACGCCGGTGACTGCCGAAGATGTCGCCTACACCTGGGCC
ACGCATGTGAAGTACGAAACCGCGGTTGCTGCTGCGAATAGGGATTACATCGAGACCATCGAAGCAGTTGATCCACAAAC
TGTTGTGATCAAGGCAAAGCTCGATGCAAACGGACGGGCTGTCAATCCTTTGATCGTCCAGGCATACCTGAGCACGTACT
ACGTCATCCAGAAAGCCTGGACGCAGAAGCTGGAAGAGCGTAGTGGTGGTGATCCTACCAAACTGTTAGCCGATCCTGCG
GAAGATGTCGTATATTCCGGTCCCTATGGCAAATTCTTTGCCGACGATACTAAGGTTGTATTGGTCCGCAACGACAATTA
CTGGGGCCAAGACCCCAGCATGTGGGGCAAGTTGCCGATGCCCAAGTACCTGGCGCACGTAATCTTCAAGGACAACGCAG
CCGGTTCAACGGCCCTGGCCGCCGGTGAAGTGGACGTCAGCCAGCAATTCAACTCTAACGTGCAGGACCTGTGGGAGAAG
CAGAATCTGCCCATCTCCACCTATCTACCCGAACCGCCCTATCACATTGGCGCTAGCCTGCCAACTGCGTTCTTTAATCT
GAAAGCTGCCGGTCTGGATCAGGTTTGCATTCGCAAGGCGATTGCAATTGCAGTTGATTATTCAGCAATCATTGCCAACG
CCATGACCAATCAGTCCGCCACTTTCGAGCAGGTGCCGCGCTCCCTGATGAACCCGACGCCCTTTGAGCAGGCGCTGTAC
AACCGCGAGGCGGTTGCACATTTGCAGTGGACGGGCAACGATATTGAAGGGGCTAAGAAGCTGCTCGATGACTGCGGGGT
TGTTGATACCGACGGCGATGGATGGCGAGAGTATAACGGTCAGAAGCTGAGCTATGTGGCCACCTGCCCCAACGGTTGGT
CTGACTGGCAAGCTGCTATCGAGATTGTCGCCGCAGCCGGCAAGCAGATTGGTATTGACATCACTACTAATTTCCCCGAG
TGGTCGGTTTATCAGACAGTCGTCACCAAATCTGACACCCCCTTACCTGCTGGCTATGAGATCTTTATGATGTGGAGCGC
AGGCGCCGGCCCGACGCAACCATGGGGACGTATTCGCAACCTGCTCAGCTCAGAGTGGATCGGCATGCCGAGCAACTGGA
GCGGTAACTGGGGCCAATACTCCAACCCAGAGGTGGATGAGCTGTTGAAATTGATCCCGCATGAGACCGATGAAGCCAGG
TTGAAAGAAATGTATACCCGGCTGGTCGAGATTTATCTGACCGACGTGCCTTCGTTCACCCTGATGTATCGCCCACAGAA
CTTCCACACTGTCAACGAAAGTATCTGGACAAACTTCCCGCATGAAGGTGATGGCACCAACCCGCCTGTCCCACCGCTTA
ACCTGATCGATGGCTGGGGCATTGCTGGTCTCTACAATCTCAGGCTTGTAGAGCCGTAG

Upstream 100 bases:

>100_bases
CAAGGAGGTGAACCAGGCCAGGACTACCAGGAGTAAATGTTGTACCTTATCGATTTATTTCCATGTTTTGGATCCCAGAC
GTTTTTTCGTGGAGGTACCT

Downstream 100 bases:

>100_bases
CGAGGCTGAAGAGACCCTTTCCGGACTATCTCCGAAAGGGTCTCTTCAAATCACTGACATGCTCAACTCACAGGCTCAAA
CGTGTTTCCAGGCATTTTGG

Product: family 5 extracellular solute-binding protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: Stage 0 sporulation protein KA [H]

Number of amino acids: Translated: 632; Mature: 632

Protein sequence:

>632_residues
MKHRFSLLIVFMVSVILTACGPSPAPVGQPTTVQPAQAPTALTEASAPTQVAAPISADALPRKETLYFNGLQWGPVVGWN
PYSNNNNNGMVIALQDSARVTMFETPYLYNMLDGKQYPLLADGDWTWNADRTEITFKIKSAARWSDGTPVTAEDVAYTWA
THVKYETAVAAANRDYIETIEAVDPQTVVIKAKLDANGRAVNPLIVQAYLSTYYVIQKAWTQKLEERSGGDPTKLLADPA
EDVVYSGPYGKFFADDTKVVLVRNDNYWGQDPSMWGKLPMPKYLAHVIFKDNAAGSTALAAGEVDVSQQFNSNVQDLWEK
QNLPISTYLPEPPYHIGASLPTAFFNLKAAGLDQVCIRKAIAIAVDYSAIIANAMTNQSATFEQVPRSLMNPTPFEQALY
NREAVAHLQWTGNDIEGAKKLLDDCGVVDTDGDGWREYNGQKLSYVATCPNGWSDWQAAIEIVAAAGKQIGIDITTNFPE
WSVYQTVVTKSDTPLPAGYEIFMMWSAGAGPTQPWGRIRNLLSSEWIGMPSNWSGNWGQYSNPEVDELLKLIPHETDEAR
LKEMYTRLVEIYLTDVPSFTLMYRPQNFHTVNESIWTNFPHEGDGTNPPVPPLNLIDGWGIAGLYNLRLVEP

Sequences:

>Translated_632_residues
MKHRFSLLIVFMVSVILTACGPSPAPVGQPTTVQPAQAPTALTEASAPTQVAAPISADALPRKETLYFNGLQWGPVVGWN
PYSNNNNNGMVIALQDSARVTMFETPYLYNMLDGKQYPLLADGDWTWNADRTEITFKIKSAARWSDGTPVTAEDVAYTWA
THVKYETAVAAANRDYIETIEAVDPQTVVIKAKLDANGRAVNPLIVQAYLSTYYVIQKAWTQKLEERSGGDPTKLLADPA
EDVVYSGPYGKFFADDTKVVLVRNDNYWGQDPSMWGKLPMPKYLAHVIFKDNAAGSTALAAGEVDVSQQFNSNVQDLWEK
QNLPISTYLPEPPYHIGASLPTAFFNLKAAGLDQVCIRKAIAIAVDYSAIIANAMTNQSATFEQVPRSLMNPTPFEQALY
NREAVAHLQWTGNDIEGAKKLLDDCGVVDTDGDGWREYNGQKLSYVATCPNGWSDWQAAIEIVAAAGKQIGIDITTNFPE
WSVYQTVVTKSDTPLPAGYEIFMMWSAGAGPTQPWGRIRNLLSSEWIGMPSNWSGNWGQYSNPEVDELLKLIPHETDEAR
LKEMYTRLVEIYLTDVPSFTLMYRPQNFHTVNESIWTNFPHEGDGTNPPVPPLNLIDGWGIAGLYNLRLVEP
>Mature_632_residues
MKHRFSLLIVFMVSVILTACGPSPAPVGQPTTVQPAQAPTALTEASAPTQVAAPISADALPRKETLYFNGLQWGPVVGWN
PYSNNNNNGMVIALQDSARVTMFETPYLYNMLDGKQYPLLADGDWTWNADRTEITFKIKSAARWSDGTPVTAEDVAYTWA
THVKYETAVAAANRDYIETIEAVDPQTVVIKAKLDANGRAVNPLIVQAYLSTYYVIQKAWTQKLEERSGGDPTKLLADPA
EDVVYSGPYGKFFADDTKVVLVRNDNYWGQDPSMWGKLPMPKYLAHVIFKDNAAGSTALAAGEVDVSQQFNSNVQDLWEK
QNLPISTYLPEPPYHIGASLPTAFFNLKAAGLDQVCIRKAIAIAVDYSAIIANAMTNQSATFEQVPRSLMNPTPFEQALY
NREAVAHLQWTGNDIEGAKKLLDDCGVVDTDGDGWREYNGQKLSYVATCPNGWSDWQAAIEIVAAAGKQIGIDITTNFPE
WSVYQTVVTKSDTPLPAGYEIFMMWSAGAGPTQPWGRIRNLLSSEWIGMPSNWSGNWGQYSNPEVDELLKLIPHETDEAR
LKEMYTRLVEIYLTDVPSFTLMYRPQNFHTVNESIWTNFPHEGDGTNPPVPPLNLIDGWGIAGLYNLRLVEP

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, It binds peptides up to five amino acids long with high affinity. Also required for sporulation and competence [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=486, Percent_Identity=24.4855967078189, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 69965; Mature: 69965

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHRFSLLIVFMVSVILTACGPSPAPVGQPTTVQPAQAPTALTEASAPTQVAAPISADAL
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCEEECCCCCCCC
PRKETLYFNGLQWGPVVGWNPYSNNNNNGMVIALQDSARVTMFETPYLYNMLDGKQYPLL
CCCCEEEECCEEECCEECCCCCCCCCCCEEEEEEECCCEEEEEECCHHHHHCCCCCCCEE
ADGDWTWNADRTEITFKIKSAARWSDGTPVTAEDVAYTWATHVKYETAVAAANRDYIETI
ECCCEEECCCCEEEEEEEECCCCCCCCCCCCHHHHEEEEHHHEEEEHHHHHCCCHHHHHH
EAVDPQTVVIKAKLDANGRAVNPLIVQAYLSTYYVIQKAWTQKLEERSGGDPTKLLADPA
HHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCH
EDVVYSGPYGKFFADDTKVVLVRNDNYWGQDPSMWGKLPMPKYLAHVIFKDNAAGSTALA
HHCEECCCCCCEECCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHEEECCCCCCCEEE
AGEVDVSQQFNSNVQDLWEKQNLPISTYLPEPPYHIGASLPTAFFNLKAAGLDQVCIRKA
ECCCCHHHHHCCCHHHHHHHCCCCEEECCCCCCCCCCCCCCHHHHEEECCCCHHHHHHHH
IAIAVDYSAIIANAMTNQSATFEQVPRSLMNPTPFEQALYNREAVAHLQWTGNDIEGAKK
HHHEECHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHCCCEEEEEEECCCCHHHHHH
LLDDCGVVDTDGDGWREYNGQKLSYVATCPNGWSDWQAAIEIVAAAGKQIGIDITTNFPE
HHHHCCEEECCCCCHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEECCCCC
WSVYQTVVTKSDTPLPAGYEIFMMWSAGAGPTQPWGRIRNLLSSEWIGMPSNWSGNWGQY
CCCEEEEEECCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
SNPEVDELLKLIPHETDEARLKEMYTRLVEIYLTDVPSFTLMYRPQNFHTVNESIWTNFP
CCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEECCHHHHCCCC
HEGDGTNPPVPPLNLIDGWGIAGLYNLRLVEP
CCCCCCCCCCCCCCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MKHRFSLLIVFMVSVILTACGPSPAPVGQPTTVQPAQAPTALTEASAPTQVAAPISADAL
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCEEECCCCCCCC
PRKETLYFNGLQWGPVVGWNPYSNNNNNGMVIALQDSARVTMFETPYLYNMLDGKQYPLL
CCCCEEEECCEEECCEECCCCCCCCCCCEEEEEEECCCEEEEEECCHHHHHCCCCCCCEE
ADGDWTWNADRTEITFKIKSAARWSDGTPVTAEDVAYTWATHVKYETAVAAANRDYIETI
ECCCEEECCCCEEEEEEEECCCCCCCCCCCCHHHHEEEEHHHEEEEHHHHHCCCHHHHHH
EAVDPQTVVIKAKLDANGRAVNPLIVQAYLSTYYVIQKAWTQKLEERSGGDPTKLLADPA
HHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCH
EDVVYSGPYGKFFADDTKVVLVRNDNYWGQDPSMWGKLPMPKYLAHVIFKDNAAGSTALA
HHCEECCCCCCEECCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHEEECCCCCCCEEE
AGEVDVSQQFNSNVQDLWEKQNLPISTYLPEPPYHIGASLPTAFFNLKAAGLDQVCIRKA
ECCCCHHHHHCCCHHHHHHHCCCCEEECCCCCCCCCCCCCCHHHHEEECCCCHHHHHHHH
IAIAVDYSAIIANAMTNQSATFEQVPRSLMNPTPFEQALYNREAVAHLQWTGNDIEGAKK
HHHEECHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHCCCEEEEEEECCCCHHHHHH
LLDDCGVVDTDGDGWREYNGQKLSYVATCPNGWSDWQAAIEIVAAAGKQIGIDITTNFPE
HHHHCCEEECCCCCHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEECCCCC
WSVYQTVVTKSDTPLPAGYEIFMMWSAGAGPTQPWGRIRNLLSSEWIGMPSNWSGNWGQY
CCCEEEEEECCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
SNPEVDELLKLIPHETDEARLKEMYTRLVEIYLTDVPSFTLMYRPQNFHTVNESIWTNFP
CCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEECCHHHHCCCC
HEGDGTNPPVPPLNLIDGWGIAGLYNLRLVEP
CCCCCCCCCCCCCCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1901616; 1899858; 9384377 [H]