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The map label for this gene is ycdT [H]

Identifier: 222524524

GI number: 222524524

Start: 1589953

End: 1590915

Strand: Reverse

Name: ycdT [H]

Synonym: Chy400_1248

Alternate gene names: 222524524

Gene position: 1590915-1589953 (Counterclockwise)

Preceding gene: 222524525

Following gene: 222524523

Centisome position: 30.19

GC content: 55.35

Gene sequence:

>963_bases
ATGCCAGACATAACAACTATTCTGCGCCGTGACCCCAACACCGGTACCGCTAACCTACTCGCTTTCGCCGAATGGCTGGC
GCAACCGACCACGCCATGTGGCCTCATCGACATTCAACTACCACATCCGCCAACACCCGCAACATTACGCTGGCTGGCCC
TCTTGTTGCGCGAAGAGCTGGCGGGAACAATCTACCAACCGGGGAATACCCAATTTGTGATCGTAGTTCCACCAGGCGCT
CCAGCCACACAGGTTGCACAGATTGAACGGTTACAGACACTCTTGCACCGGCAGGCACAACGTCTCGATCTGAGCACAAT
ACCGGTTATTCGCTACCATCACGTTCCCGCGGACAGTGACCCGGCGACGATTCTGCAATTTCTGATCCCCGATACCGCCG
ATCCGGGGACACTGCTCAACCGATTGATTGCTCAACTCGCAGCGTTAGGGGCTACGCTCAATGATCAGCATCAATTCGAT
CTCGACCCGGTTACCGGTTTGCCAAATAGCTTTGCTGCCCGGCAACGCCTTGAGGAGCTGATTCTAACAGCGCAGAGACA
AAGTCAGGCGTGTAGTGTGTTGCTGATGAATGGTGATGACCTCCGTCGCTACAACGAAATGAGTTATGCTGCCGGCGATC
AGATGATCCGCGATCTGGCTACCGTCCTCCGTGAACAATTACGACCGAGTGATTTTCTCGCCCGCTGGCAGACGGGAGAC
CAGTTTCTGGCGGTATTACCCAACACCGGTGTAAGTGGAGCGGCTGCACTGGCTGAACGATTACGCCAGCGTGTCATCGA
ACGATCACAGCTCTGGCCGTTTCCAGTCACAATTTCGGTTGGAATTGCCTCGATCCCACAACACGGTCATACTGCCGGGG
TTGCGCTGCAAATTGCCACCACAGCACTCTTACAGGCGAAAGCCGCCGGCAAAAATCGGATCGTTGTCGGACGACCAGGA
TAA

Upstream 100 bases:

>100_bases
TCACCAAAATGACTCGTATAATGAGATAGCATACCATCAAACGACAATCGGGCCAACATCATGCCGGGTTGGCACTATTG
AATAAAGCTGAGAGCAACCT

Downstream 100 bases:

>100_bases
CTACCGACACGGGTAGTAATATACCGACAAAACATAAGTCAGCGTGGTACAATATAAAATAAAGAATAATCTGCATCGTT
TCGGTTTCGGTAGGCGCCTA

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MPDITTILRRDPNTGTANLLAFAEWLAQPTTPCGLIDIQLPHPPTPATLRWLALLLREELAGTIYQPGNTQFVIVVPPGA
PATQVAQIERLQTLLHRQAQRLDLSTIPVIRYHHVPADSDPATILQFLIPDTADPGTLLNRLIAQLAALGATLNDQHQFD
LDPVTGLPNSFAARQRLEELILTAQRQSQACSVLLMNGDDLRRYNEMSYAAGDQMIRDLATVLREQLRPSDFLARWQTGD
QFLAVLPNTGVSGAAALAERLRQRVIERSQLWPFPVTISVGIASIPQHGHTAGVALQIATTALLQAKAAGKNRIVVGRPG

Sequences:

>Translated_320_residues
MPDITTILRRDPNTGTANLLAFAEWLAQPTTPCGLIDIQLPHPPTPATLRWLALLLREELAGTIYQPGNTQFVIVVPPGA
PATQVAQIERLQTLLHRQAQRLDLSTIPVIRYHHVPADSDPATILQFLIPDTADPGTLLNRLIAQLAALGATLNDQHQFD
LDPVTGLPNSFAARQRLEELILTAQRQSQACSVLLMNGDDLRRYNEMSYAAGDQMIRDLATVLREQLRPSDFLARWQTGD
QFLAVLPNTGVSGAAALAERLRQRVIERSQLWPFPVTISVGIASIPQHGHTAGVALQIATTALLQAKAAGKNRIVVGRPG
>Mature_319_residues
PDITTILRRDPNTGTANLLAFAEWLAQPTTPCGLIDIQLPHPPTPATLRWLALLLREELAGTIYQPGNTQFVIVVPPGAP
ATQVAQIERLQTLLHRQAQRLDLSTIPVIRYHHVPADSDPATILQFLIPDTADPGTLLNRLIAQLAALGATLNDQHQFDL
DPVTGLPNSFAARQRLEELILTAQRQSQACSVLLMNGDDLRRYNEMSYAAGDQMIRDLATVLREQLRPSDFLARWQTGDQ
FLAVLPNTGVSGAAALAERLRQRVIERSQLWPFPVTISVGIASIPQHGHTAGVALQIATTALLQAKAAGKNRIVVGRPG

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=179, Percent_Identity=31.2849162011173, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787541, Length=161, Percent_Identity=31.6770186335404, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1788381, Length=162, Percent_Identity=30.2469135802469, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI145693134, Length=184, Percent_Identity=27.7173913043478, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87081921, Length=208, Percent_Identity=29.3269230769231, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI87082007, Length=183, Percent_Identity=28.4153005464481, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 34921; Mature: 34789

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDITTILRRDPNTGTANLLAFAEWLAQPTTPCGLIDIQLPHPPTPATLRWLALLLREEL
CCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
AGTIYQPGNTQFVIVVPPGAPATQVAQIERLQTLLHRQAQRLDLSTIPVIRYHHVPADSD
CCCEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
PATILQFLIPDTADPGTLLNRLIAQLAALGATLNDQHQFDLDPVTGLPNSFAARQRLEEL
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ILTAQRQSQACSVLLMNGDDLRRYNEMSYAAGDQMIRDLATVLREQLRPSDFLARWQTGD
HHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCC
QFLAVLPNTGVSGAAALAERLRQRVIERSQLWPFPVTISVGIASIPQHGHTAGVALQIAT
CEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHCCCCCCCHHHHHHHHH
TALLQAKAAGKNRIVVGRPG
HHHHHHHCCCCCEEEEECCC
>Mature Secondary Structure 
PDITTILRRDPNTGTANLLAFAEWLAQPTTPCGLIDIQLPHPPTPATLRWLALLLREEL
CCHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
AGTIYQPGNTQFVIVVPPGAPATQVAQIERLQTLLHRQAQRLDLSTIPVIRYHHVPADSD
CCCEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
PATILQFLIPDTADPGTLLNRLIAQLAALGATLNDQHQFDLDPVTGLPNSFAARQRLEEL
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ILTAQRQSQACSVLLMNGDDLRRYNEMSYAAGDQMIRDLATVLREQLRPSDFLARWQTGD
HHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCC
QFLAVLPNTGVSGAAALAERLRQRVIERSQLWPFPVTISVGIASIPQHGHTAGVALQIAT
CEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHCCCCCCCHHHHHHHHH
TALLQAKAAGKNRIVVGRPG
HHHHHHHCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]