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The map label for this gene is yisK [H]

Identifier: 222524498

GI number: 222524498

Start: 1557666

End: 1558529

Strand: Reverse

Name: yisK [H]

Synonym: Chy400_1222

Alternate gene names: 222524498

Gene position: 1558529-1557666 (Counterclockwise)

Preceding gene: 222524499

Following gene: 222524497

Centisome position: 29.58

GC content: 57.87

Gene sequence:

>864_bases
ATGAAGCTCTTAACCTATCGGTATGGTGGGGTTGAACGGGTTGGTGCCCTGCGGGGCGATGAGGTGATCGATCTGTCGGC
GGTGGCAGCGTCCATGCTCGATCTGATCGATGGTGGGCCAGAGTTACTGGCTGCGGCACAGCAGGTCGTCGCTACGGCGG
AAGGAGGGGTGGCGCTGGCTGCGGTTGAGTTGCGTGCGCCAATTCCGCGCCCGCGCAAGAACATCATCTGTCTGGGGATG
AATTATGCTGCTCATGCGATTGAGTCGTTGCGGGCGAAGGGGTTGCCGGAAAAGCTGCCCGAATACCCCGTCTTCTTCAG
TAAGATGCCCACGGCAGTCAATCATCCCAATGCACCGGTACCGTTGATGGCTGATGTCTCGGCGCAGCGCGACTGGGAGG
TTGAGCTGGCGGTGATTATCGGGCGGCGTGGGCGGAATATTCCGGCAAGTGCTGCGCTCGATTACGTCTTCGGCTATACC
ATTATGAATGATGTGAGCGCACGCGATCTTCAGACGCGCCATCAACAGTTTTTCTACAGCAAGAGCCTCGACGGTAGTGC
CCCGCTGGGACCGTGGATTGTCACCGCTGATGAAATTCCCGATCCACACGCGCTCGGCATCCGGCTGCGCCTCAATGGCG
AGCTGGTACAAAATTCGGTGACGCGCGATATGATCTTTGATATTCCCACCTGTATTGCCACCTTCAGCCGTGGCGTGACG
CTCGAACCGGGTGATATTATTGCGACCGGTACACCTTCGGGGGTCGGCATGGGCATGACGCCACAACGCTGGTTGAAGGT
AGGCGATGTGATGGAGGCCGAGATTGATGGGATTGGGGTGCTGAAGAATGTTGTAAGTGAGTAG

Upstream 100 bases:

>100_bases
CGCTGGCTGGCGTATCTTGGTCTCTTGACGTTTATTCTGGTCTTCACACCGGTTCCGATTACGGTCATGACGCCGTAAGT
GTGGGTGATACGGAGGTCAC

Downstream 100 bases:

>100_bases
ACCAGGTTGCCCCTATTCCATATCCACGGCAGACGAATGCACAGAGCATTTTTCTGTGCATTCGTGCTTTAAAACCTACG
TACCCGGCATAGTTCAGCCA

Product: 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MKLLTYRYGGVERVGALRGDEVIDLSAVAASMLDLIDGGPELLAAAQQVVATAEGGVALAAVELRAPIPRPRKNIICLGM
NYAAHAIESLRAKGLPEKLPEYPVFFSKMPTAVNHPNAPVPLMADVSAQRDWEVELAVIIGRRGRNIPASAALDYVFGYT
IMNDVSARDLQTRHQQFFYSKSLDGSAPLGPWIVTADEIPDPHALGIRLRLNGELVQNSVTRDMIFDIPTCIATFSRGVT
LEPGDIIATGTPSGVGMGMTPQRWLKVGDVMEAEIDGIGVLKNVVSE

Sequences:

>Translated_287_residues
MKLLTYRYGGVERVGALRGDEVIDLSAVAASMLDLIDGGPELLAAAQQVVATAEGGVALAAVELRAPIPRPRKNIICLGM
NYAAHAIESLRAKGLPEKLPEYPVFFSKMPTAVNHPNAPVPLMADVSAQRDWEVELAVIIGRRGRNIPASAALDYVFGYT
IMNDVSARDLQTRHQQFFYSKSLDGSAPLGPWIVTADEIPDPHALGIRLRLNGELVQNSVTRDMIFDIPTCIATFSRGVT
LEPGDIIATGTPSGVGMGMTPQRWLKVGDVMEAEIDGIGVLKNVVSE
>Mature_287_residues
MKLLTYRYGGVERVGALRGDEVIDLSAVAASMLDLIDGGPELLAAAQQVVATAEGGVALAAVELRAPIPRPRKNIICLGM
NYAAHAIESLRAKGLPEKLPEYPVFFSKMPTAVNHPNAPVPLMADVSAQRDWEVELAVIIGRRGRNIPASAALDYVFGYT
IMNDVSARDLQTRHQQFFYSKSLDGSAPLGPWIVTADEIPDPHALGIRLRLNGELVQNSVTRDMIFDIPTCIATFSRGVT
LEPGDIIATGTPSGVGMGMTPQRWLKVGDVMEAEIDGIGVLKNVVSE

Specific function: Unknown

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI156231349, Length=273, Percent_Identity=39.5604395604396, Blast_Score=185, Evalue=4e-47,
Organism=Homo sapiens, GI40786394, Length=273, Percent_Identity=39.5604395604396, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI215422413, Length=210, Percent_Identity=40.9523809523809, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI13654274, Length=210, Percent_Identity=40.9523809523809, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI66348062, Length=210, Percent_Identity=40.9523809523809, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1787428, Length=219, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17557057, Length=203, Percent_Identity=36.9458128078818, Blast_Score=121, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6324161, Length=238, Percent_Identity=26.4705882352941, Blast_Score=72, Evalue=8e-14,
Organism=Drosophila melanogaster, GI28572127, Length=297, Percent_Identity=38.3838383838384, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24663695, Length=292, Percent_Identity=32.5342465753425, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI28571789, Length=205, Percent_Identity=30.7317073170732, Blast_Score=98, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: NA

Molecular weight: Translated: 30857; Mature: 30857

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLTYRYGGVERVGALRGDEVIDLSAVAASMLDLIDGGPELLAAAQQVVATAEGGVALA
CEEEEEECCCHHHHHCCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEE
AVELRAPIPRPRKNIICLGMNYAAHAIESLRAKGLPEKLPEYPVFFSKMPTAVNHPNAPV
EEEEECCCCCCCCCEEEEECCHHHHHHHHHHHCCCHHHCCCCCCHHHHCCCCCCCCCCCC
PLMADVSAQRDWEVELAVIIGRRGRNIPASAALDYVFGYTIMNDVSARDLQTRHQQFFYS
CEEECCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
KSLDGSAPLGPWIVTADEIPDPHALGIRLRLNGELVQNSVTRDMIFDIPTCIATFSRGVT
CCCCCCCCCCCEEEEECCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCE
LEPGDIIATGTPSGVGMGMTPQRWLKVGDVMEAEIDGIGVLKNVVSE
ECCCCEEEECCCCCCCCCCCHHHHHHHCCHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MKLLTYRYGGVERVGALRGDEVIDLSAVAASMLDLIDGGPELLAAAQQVVATAEGGVALA
CEEEEEECCCHHHHHCCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEE
AVELRAPIPRPRKNIICLGMNYAAHAIESLRAKGLPEKLPEYPVFFSKMPTAVNHPNAPV
EEEEECCCCCCCCCEEEEECCHHHHHHHHHHHCCCHHHCCCCCCHHHHCCCCCCCCCCCC
PLMADVSAQRDWEVELAVIIGRRGRNIPASAALDYVFGYTIMNDVSARDLQTRHQQFFYS
CEEECCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
KSLDGSAPLGPWIVTADEIPDPHALGIRLRLNGELVQNSVTRDMIFDIPTCIATFSRGVT
CCCCCCCCCCCEEEEECCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCE
LEPGDIIATGTPSGVGMGMTPQRWLKVGDVMEAEIDGIGVLKNVVSE
ECCCCEEEECCCCCCCCCCCHHHHHHHCCHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377 [H]