Click here to switch to the map view.

The map label for this gene is braE [H]

Identifier: 222524461

GI number: 222524461

Start: 1505374

End: 1506432

Strand: Reverse

Name: braE [H]

Synonym: Chy400_1185

Alternate gene names: 222524461

Gene position: 1506432-1505374 (Counterclockwise)

Preceding gene: 222524462

Following gene: 222524460

Centisome position: 28.59

GC content: 54.58

Gene sequence:

>1059_bases
ATGCAGAGCGGAACATTTCATACCACGTATGCCAGTGATGTCAGTCTGCGCCCCTATTGGCCGCAACGGATGCGGATTGT
CCTTGTTCTGGTGGCAGTGCTGATCTTTCCCTGGTTTGCCGACCGCTACTGGCTCAATCTGGCCAACACGATTGCCATCG
CTGCAATCGGTGCTATCGGCCTGAACATTCTGGTCGGTTACACCGGACAGATCAGTATCGGGCATGGTGGCTTTCTGGCG
GTCGGGGCGTATACCGCCGGTTTGCTGGCCGTGCATCTTGGTACCCCGATGTGGCTGACCATTCCTATCGCCAGCTTTTT
CACTGCGGCAGTTGGTGCCTTCTTCGGTCTGCCGTCACTGCGCCTGAAAGGTCTGTATCTGGCGATTGCCACCCTCGCGT
CGCAAGAGATTATCATCTGGCTCCTGACACACGGCAAACTGCTCGGCATCGGCGAGTCACTTTCGTTACCGCGGGCGACC
AATAATCTCTTCGGTCTGCCAATGGAGTGGCTCGGTAACAATGATTTTGCCTTCTACTGGGTGTGTCTCGCCTTTTTGAT
CCTGACGGTTATCTTCGTGAGCAATCTGTTCCGAACCCGTACCGGGCGCGCCTTTGTTGCGATCCGCGATCAAGACATTG
CGGCTCAGGTGATCGGTGTCGATCTCTTTCGGTATAAACTCCTGGCCTTCGCCACGTCTTCATTCTTCGCCGGTCTGGCC
GGTGCGCTGACTGCGCACTACCGTGGTGTGATCTCGTGGGAGCGGTTTACTATCGAAACCAGCATTCTCTACCTGGCGAT
GATTATTATCGGCGGTTTAGGGAGCGTTTCAGGATCAATCTACGGCGCTGCATTCATGATCTTGCTCCCAGCCTTTCTGT
CGAATATGGCCCGCACGCTGAGTGGCGTCGTTCCCGACATCAACTCGTATCTGCCGTATATTCAGCAGGGTGCTTTTGGT
CTGGCAATCATCCTCTTCCTCATCTTCGAGCCGGAAGGCATCGTTAAGATGTGGCGCAATGTTAAAGATTATTTCCGGCT
ATGGCCGTTCAGTTACTGA

Upstream 100 bases:

>100_bases
CATCCTCTTGTTCATTCTGCTGGTACGTCCTTACGGTCTCTTCGGACAGCGCATTATCGAGCGGGTGTAGGGGATACAGA
CCATACAGACAGGGTTGCCT

Downstream 100 bases:

>100_bases
GGTGTGTAGCAAGGAGGATGGCATGCACAGGTGGAAACGCACGTTCGGTTGGTGGATGGTAATGGCGTTGGTTGCCACAA
TGCTGGCAGCATGTGGTGGC

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MQSGTFHTTYASDVSLRPYWPQRMRIVLVLVAVLIFPWFADRYWLNLANTIAIAAIGAIGLNILVGYTGQISIGHGGFLA
VGAYTAGLLAVHLGTPMWLTIPIASFFTAAVGAFFGLPSLRLKGLYLAIATLASQEIIIWLLTHGKLLGIGESLSLPRAT
NNLFGLPMEWLGNNDFAFYWVCLAFLILTVIFVSNLFRTRTGRAFVAIRDQDIAAQVIGVDLFRYKLLAFATSSFFAGLA
GALTAHYRGVISWERFTIETSILYLAMIIIGGLGSVSGSIYGAAFMILLPAFLSNMARTLSGVVPDINSYLPYIQQGAFG
LAIILFLIFEPEGIVKMWRNVKDYFRLWPFSY

Sequences:

>Translated_352_residues
MQSGTFHTTYASDVSLRPYWPQRMRIVLVLVAVLIFPWFADRYWLNLANTIAIAAIGAIGLNILVGYTGQISIGHGGFLA
VGAYTAGLLAVHLGTPMWLTIPIASFFTAAVGAFFGLPSLRLKGLYLAIATLASQEIIIWLLTHGKLLGIGESLSLPRAT
NNLFGLPMEWLGNNDFAFYWVCLAFLILTVIFVSNLFRTRTGRAFVAIRDQDIAAQVIGVDLFRYKLLAFATSSFFAGLA
GALTAHYRGVISWERFTIETSILYLAMIIIGGLGSVSGSIYGAAFMILLPAFLSNMARTLSGVVPDINSYLPYIQQGAFG
LAIILFLIFEPEGIVKMWRNVKDYFRLWPFSY
>Mature_352_residues
MQSGTFHTTYASDVSLRPYWPQRMRIVLVLVAVLIFPWFADRYWLNLANTIAIAAIGAIGLNILVGYTGQISIGHGGFLA
VGAYTAGLLAVHLGTPMWLTIPIASFFTAAVGAFFGLPSLRLKGLYLAIATLASQEIIIWLLTHGKLLGIGESLSLPRAT
NNLFGLPMEWLGNNDFAFYWVCLAFLILTVIFVSNLFRTRTGRAFVAIRDQDIAAQVIGVDLFRYKLLAFATSSFFAGLA
GALTAHYRGVISWERFTIETSILYLAMIIIGGLGSVSGSIYGAAFMILLPAFLSNMARTLSGVVPDINSYLPYIQQGAFG
LAIILFLIFEPEGIVKMWRNVKDYFRLWPFSY

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=324, Percent_Identity=34.2592592592593, Blast_Score=137, Evalue=8e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 38740; Mature: 38740

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSGTFHTTYASDVSLRPYWPQRMRIVLVLVAVLIFPWFADRYWLNLANTIAIAAIGAIG
CCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNILVGYTGQISIGHGGFLAVGAYTAGLLAVHLGTPMWLTIPIASFFTAAVGAFFGLPSL
HEEEEECCCEEEECCCCEEHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCH
RLKGLYLAIATLASQEIIIWLLTHGKLLGIGESLSLPRATNNLFGLPMEWLGNNDFAFYW
HHHHHHHHHHHHCCCCEEEEEEECCEEEECCCCCCCCCCCCCCCCCCHHHCCCCCHHHHH
VCLAFLILTVIFVSNLFRTRTGRAFVAIRDQDIAAQVIGVDLFRYKLLAFATSSFFAGLA
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GALTAHYRGVISWERFTIETSILYLAMIIIGGLGSVSGSIYGAAFMILLPAFLSNMARTL
HHHHHHHHCEEEEEHEEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SGVVPDINSYLPYIQQGAFGLAIILFLIFEPEGIVKMWRNVKDYFRLWPFSY
HCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQSGTFHTTYASDVSLRPYWPQRMRIVLVLVAVLIFPWFADRYWLNLANTIAIAAIGAIG
CCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNILVGYTGQISIGHGGFLAVGAYTAGLLAVHLGTPMWLTIPIASFFTAAVGAFFGLPSL
HEEEEECCCEEEECCCCEEHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCH
RLKGLYLAIATLASQEIIIWLLTHGKLLGIGESLSLPRATNNLFGLPMEWLGNNDFAFYW
HHHHHHHHHHHHCCCCEEEEEEECCEEEECCCCCCCCCCCCCCCCCCHHHCCCCCHHHHH
VCLAFLILTVIFVSNLFRTRTGRAFVAIRDQDIAAQVIGVDLFRYKLLAFATSSFFAGLA
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GALTAHYRGVISWERFTIETSILYLAMIIIGGLGSVSGSIYGAAFMILLPAFLSNMARTL
HHHHHHHHCEEEEEHEEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SGVVPDINSYLPYIQQGAFGLAIILFLIFEPEGIVKMWRNVKDYFRLWPFSY
HCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]