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The map label for this gene is bglA [H]

Identifier: 222524452

GI number: 222524452

Start: 1493452

End: 1494276

Strand: Reverse

Name: bglA [H]

Synonym: Chy400_1176

Alternate gene names: 222524452

Gene position: 1494276-1493452 (Counterclockwise)

Preceding gene: 222524453

Following gene: 222524451

Centisome position: 28.36

GC content: 53.94

Gene sequence:

>825_bases
ATGATCAGGCGACAGATAGTGTGGCTGGGAATAGTGTGTCTGTTCCTGGTTGGATGCTCTGCTATGCCGACGACGCCTGT
GATCGGTGAACCGACCCCAACCCCCTCACCGTGGACGCTCGTCTGGAGTGATGAGTTTGAGAGCGAGACGCTCAACCCGC
AGAACTGGTTGTTCGACAAAGGGGCCGGGGGATGGGGGAATAACGAATTGCAGTACTACAGCAATCGGCCAGAGAATATC
CGTATTGAAGATGGTGTCTTGATTATCGAAGCCCGTAATGAAGAGTACATGGCCTGGGAATATACTTCTGCCCGCATCAA
GACCCACTATCTGCAGAGCTGGACGTATGGTCGGATCGAGGCGCGCATGAAGCTCCCGGTTGGTGGTAAAGGCATCTGGC
CGGCCTTCTGGATGTTAGGCGAAAACATCGCTACTGCGCGCTGGCCCAACTGTGGCGAGATCGACATTATGGAAAATATC
GGTAATCCAACCGTTGTTTATGGTTCGGTACACGGCCCAGGCTATTCTGGTGGAAATGCGATCACAAAGTCATTTGTCAG
TGCAGTGGATCTGTCGGCTGATTTTCACATCTACGCTGTCGAATGGGAGGCCGACGAGATCCGCTGGTACGTCGATGATC
AACTCTACCACACCCTGCGTCGTGAACAGGTACCGGGTGAATGGGTGTTTGATCATCCGTTCTTTCTCATTCTCAATCTA
GCCGTCGGCGGGAACTGGCCGGGGTACCCCGACGAAACAACCGTCTTTCCCCAGCAGCTCCTGGTTGATTATGTGCGTGT
GTATCAAGCTGGTGCGGCCTCGTGA

Upstream 100 bases:

>100_bases
CTGACCGGCGATGTTGCCCTCTACTGATAACGAGCCGGTCTTTATGTGAAACGTTGGTGGTCTGGAAAGGTTGTAGACCG
TGCAGACATTATTGTCACCC

Downstream 100 bases:

>100_bases
GCCGGCCTATTCAGAGGAAGGTTATGACTACACTCGTTGATCCAGAAACACTGCTTGCTCACCTGACTCTTGACGAAAAG
ATTGCCCTGCTTGCCGGTGC

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: 1,3-1,4-beta-D-glucan 4-glucanohydrolase; Endo-beta-1,3-1,4 glucanase; Lichenase [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MIRRQIVWLGIVCLFLVGCSAMPTTPVIGEPTPTPSPWTLVWSDEFESETLNPQNWLFDKGAGGWGNNELQYYSNRPENI
RIEDGVLIIEARNEEYMAWEYTSARIKTHYLQSWTYGRIEARMKLPVGGKGIWPAFWMLGENIATARWPNCGEIDIMENI
GNPTVVYGSVHGPGYSGGNAITKSFVSAVDLSADFHIYAVEWEADEIRWYVDDQLYHTLRREQVPGEWVFDHPFFLILNL
AVGGNWPGYPDETTVFPQQLLVDYVRVYQAGAAS

Sequences:

>Translated_274_residues
MIRRQIVWLGIVCLFLVGCSAMPTTPVIGEPTPTPSPWTLVWSDEFESETLNPQNWLFDKGAGGWGNNELQYYSNRPENI
RIEDGVLIIEARNEEYMAWEYTSARIKTHYLQSWTYGRIEARMKLPVGGKGIWPAFWMLGENIATARWPNCGEIDIMENI
GNPTVVYGSVHGPGYSGGNAITKSFVSAVDLSADFHIYAVEWEADEIRWYVDDQLYHTLRREQVPGEWVFDHPFFLILNL
AVGGNWPGYPDETTVFPQQLLVDYVRVYQAGAAS
>Mature_274_residues
MIRRQIVWLGIVCLFLVGCSAMPTTPVIGEPTPTPSPWTLVWSDEFESETLNPQNWLFDKGAGGWGNNELQYYSNRPENI
RIEDGVLIIEARNEEYMAWEYTSARIKTHYLQSWTYGRIEARMKLPVGGKGIWPAFWMLGENIATARWPNCGEIDIMENI
GNPTVVYGSVHGPGYSGGNAITKSFVSAVDLSADFHIYAVEWEADEIRWYVDDQLYHTLRREQVPGEWVFDHPFFLILNL
AVGGNWPGYPDETTVFPQQLLVDYVRVYQAGAAS

Specific function: Shows activity on lichenan, beta-glucan and laminarin but not on CMC cellulose or xylan [H]

COG id: COG2273

COG function: function code G; Beta-glucanase/Beta-glucan synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 16 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR013320
- InterPro:   IPR000757
- InterPro:   IPR008263 [H]

Pfam domain/function: PF00722 Glyco_hydro_16 [H]

EC number: =3.2.1.73 [H]

Molecular weight: Translated: 31162; Mature: 31162

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01034 GLYCOSYL_HYDROL_F16

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRRQIVWLGIVCLFLVGCSAMPTTPVIGEPTPTPSPWTLVWSDEFESETLNPQNWLFDK
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEC
GAGGWGNNELQYYSNRPENIRIEDGVLIIEARNEEYMAWEYTSARIKTHYLQSWTYGRIE
CCCCCCCCCEEEECCCCCEEEEECCEEEEEECCCCEEEEEECCHHEEEEHHHCCCCEEEE
ARMKLPVGGKGIWPAFWMLGENIATARWPNCGEIDIMENIGNPTVVYGSVHGPGYSGGNA
EEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEHHHCCCCCEEEEECCCCCCCCCCHH
ITKSFVSAVDLSADFHIYAVEWEADEIRWYVDDQLYHTLRREQVPGEWVFDHPFFLILNL
HHHHHHHHHCCCCCEEEEEEEEECCCEEEEECHHHHHHHHHHCCCCCEEECCCEEEEEEE
AVGGNWPGYPDETTVFPQQLLVDYVRVYQAGAAS
EECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIRRQIVWLGIVCLFLVGCSAMPTTPVIGEPTPTPSPWTLVWSDEFESETLNPQNWLFDK
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEC
GAGGWGNNELQYYSNRPENIRIEDGVLIIEARNEEYMAWEYTSARIKTHYLQSWTYGRIE
CCCCCCCCCEEEECCCCCEEEEECCEEEEEECCCCEEEEEECCHHEEEEHHHCCCCEEEE
ARMKLPVGGKGIWPAFWMLGENIATARWPNCGEIDIMENIGNPTVVYGSVHGPGYSGGNA
EEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEHHHCCCCCEEEEECCCCCCCCCCHH
ITKSFVSAVDLSADFHIYAVEWEADEIRWYVDDQLYHTLRREQVPGEWVFDHPFFLILNL
HHHHHHHHHCCCCCEEEEEEEEECCCEEEEECHHHHHHHHHHCCCCCEEECCCEEEEEEE
AVGGNWPGYPDETTVFPQQLLVDYVRVYQAGAAS
EECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7925416 [H]