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The map label for this gene is yoaE [H]

Identifier: 222524389

GI number: 222524389

Start: 1410005

End: 1412074

Strand: Direct

Name: yoaE [H]

Synonym: Chy400_1113

Alternate gene names: 222524389

Gene position: 1410005-1412074 (Clockwise)

Preceding gene: 222524387

Following gene: 222524390

Centisome position: 26.76

GC content: 58.55

Gene sequence:

>2070_bases
ATGACAACACAACGTGTTCGTGGTTGCTGCCCGCATGATTGCCCCGATACCTGTGCCACCATAACGGAAGTGCGTGATGG
ACGGGCAGTCGCGTTTTATGCCGATCCTGAACATCCGATCACCCAGGGCTGGCTGTGTGCCAAAGTCCGTCCGTACCTCG
AACGAGTGTACAGTCCTGATCGGCTGCTCTACCCACTCCGTCGGGTAGGACCAAAAGGCAGCGGACGCTGGGAACGGATA
AGCTGGGACGAAGCGATCACTACCATCGCAGAGCGCTGGCAGGCAATTATCGCCGAATACGGTGCTGCTGCGATCCTGCC
CTATTCGTACAGCGGCACGCTGGGTTTGCTGCAAACGGCGATTGTTGATGCCCGCTTGTGGAACCGGATGGGAGCCAGCG
GTCTGGATCGAACGATCTGCTGTGCTGCTGCCCACGCTGCTGTCTATGCCACGCTCGGTGCCCGCCGCAGTCCTGACTAC
GACGACCTGCGGCACAGCCGGCTCATAATCCTTTGGGGACATAATCCGGCCAGTGCCGGCCCTCACGCAATGCCGTTTAT
CCGTCAGGCACAGCGTGCCGGTGCCTATGTGGTGGTGATCGATCCACGACGGACGGCTACCGCTCGCTCTGCCGATCTCC
ATCTGGCGATCAGGCCGGCAACCGATGGGGCGCTGGCCTTGGGCATGATGAATGTCATCTTCGCTGAAAACCTGCACGAT
GAGGCATGGTTAGAAGCGCATACGGTTGGCTGGCGTGAATTGCGCGCGCGGGCTGCCGAATATGATCCCGAACGGGTAGC
AACCATTACCGGACTGCCGGTATCAACCATCATTGAACTGGCCCGTCGCTATGCAACCACCAAACCGGCTATCATTAAAA
CAGCCGACGGTATTCAACGCCATCAGAATGGTGGTCAGACCTTCCGGGCACTCCTTTGCCTGCCGGCGGTTGTGGGACAG
TACGGTGTGCGTGGCGGTGGCCTGGCCTATTCCACCGGCGGTTATGCAACGTGGGATGCGGAAGCCGTTGGTCATGCCTC
TGAATGCCCACCAGTACCGCGGACTGTCAATATGAACCGTCTTGGCGCGGCTCTCACCGGCGAAGTAACCGATCCGCCGA
TCATGTCCCTCTTTGTCTACTGCGCTAACCCTCTCGCTTCATCACCCAATGCCGGCCTCATCGAACAGGGGTTGCGGCGT
GAAGACCTCTTTACCGTCGTCCACGAACTCTTTATGACCGATACGGCCCGCTACGCCGATATTGTCTTGCCGGCAACCAG
TCAACTCGAACACCTCGATCTGCACCGTGCCTATGGCCATCGTTATCTCACGCTTAACCAACCGGCAATTGCCCCCTTGG
GGGAAGCGAAGAGCAATTGGGACGTGACCCGCTTGCTGGCGCAGGCGATGGGCTATAGCGAACCGTGGCTGCACGAGAGT
GCGGAAGAGGCGATCCGTGGTGTCCTCGATGCCTCGCGGGCACGCAATCCATTTCTGGCCGGCATCACCTTTGAACGTCT
GCAACAAGAGGGAACCATCCGGCTCACACTGTCACCAGAGAACGAAGTGCCGTTTGCTGATGGTCACTTCCCAACCCCAT
CAGGCAAAGTTGAATTGTGGAGTGCGACCATGGCGGCTCAGGGGCTTGATCCGCTTCCCCATTATGAACCACCGGCTGAG
TATGTCGCACATCCGCCGGAGGAGGGATGGCTAACCCTCATCTCAGCCGCTGCTCACCATTTTGTCTCGTCAAGTATGGC
CAACCAGCCCAGTTTGCGGGCAAAAGAGGGAGAACCGACTATCGAGATTAATCCGGTCGATGCTGCCGCGCGCAACATTC
GTGATGGTGACGTTGTCGTTGTCAGTAATCAACGAGGGCAGTGCCATCTGCGTGCAGTTGTGAGTACCAACGTACCGCCC
GGTGTTGTCTCTGCGGTCAAGGGCCACTGGCCTTCACTCTCGCCCGATGGGCGAAATGTCAACTGGACAACTCCCGATGC
ACTGGCCGATCTGGGTGGACAGAGCACATTTCACAGTAATCGGGTACAGGTCTGGCCATCACGCATCTGA

Upstream 100 bases:

>100_bases
CACGACCAGATTTGATATCATCCAGTTCTGAAGCGGATTCTGGTCACAACAATTTGAGAGTGCTACAATACGTAAAATAA
CCGCTAGCGGAGAGATTGCT

Downstream 100 bases:

>100_bases
CAGCATCGTGACATTCCCTTCACAGCACAACCATGAAAGGAGTGGGTGTCGTGAAGCGGCTCGATTGGGGAAAAACCGGT
CAGTCGATGACTGAAATTAC

Product: molybdopterin oxidoreductase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 689; Mature: 688

Protein sequence:

>689_residues
MTTQRVRGCCPHDCPDTCATITEVRDGRAVAFYADPEHPITQGWLCAKVRPYLERVYSPDRLLYPLRRVGPKGSGRWERI
SWDEAITTIAERWQAIIAEYGAAAILPYSYSGTLGLLQTAIVDARLWNRMGASGLDRTICCAAAHAAVYATLGARRSPDY
DDLRHSRLIILWGHNPASAGPHAMPFIRQAQRAGAYVVVIDPRRTATARSADLHLAIRPATDGALALGMMNVIFAENLHD
EAWLEAHTVGWRELRARAAEYDPERVATITGLPVSTIIELARRYATTKPAIIKTADGIQRHQNGGQTFRALLCLPAVVGQ
YGVRGGGLAYSTGGYATWDAEAVGHASECPPVPRTVNMNRLGAALTGEVTDPPIMSLFVYCANPLASSPNAGLIEQGLRR
EDLFTVVHELFMTDTARYADIVLPATSQLEHLDLHRAYGHRYLTLNQPAIAPLGEAKSNWDVTRLLAQAMGYSEPWLHES
AEEAIRGVLDASRARNPFLAGITFERLQQEGTIRLTLSPENEVPFADGHFPTPSGKVELWSATMAAQGLDPLPHYEPPAE
YVAHPPEEGWLTLISAAAHHFVSSSMANQPSLRAKEGEPTIEINPVDAAARNIRDGDVVVVSNQRGQCHLRAVVSTNVPP
GVVSAVKGHWPSLSPDGRNVNWTTPDALADLGGQSTFHSNRVQVWPSRI

Sequences:

>Translated_689_residues
MTTQRVRGCCPHDCPDTCATITEVRDGRAVAFYADPEHPITQGWLCAKVRPYLERVYSPDRLLYPLRRVGPKGSGRWERI
SWDEAITTIAERWQAIIAEYGAAAILPYSYSGTLGLLQTAIVDARLWNRMGASGLDRTICCAAAHAAVYATLGARRSPDY
DDLRHSRLIILWGHNPASAGPHAMPFIRQAQRAGAYVVVIDPRRTATARSADLHLAIRPATDGALALGMMNVIFAENLHD
EAWLEAHTVGWRELRARAAEYDPERVATITGLPVSTIIELARRYATTKPAIIKTADGIQRHQNGGQTFRALLCLPAVVGQ
YGVRGGGLAYSTGGYATWDAEAVGHASECPPVPRTVNMNRLGAALTGEVTDPPIMSLFVYCANPLASSPNAGLIEQGLRR
EDLFTVVHELFMTDTARYADIVLPATSQLEHLDLHRAYGHRYLTLNQPAIAPLGEAKSNWDVTRLLAQAMGYSEPWLHES
AEEAIRGVLDASRARNPFLAGITFERLQQEGTIRLTLSPENEVPFADGHFPTPSGKVELWSATMAAQGLDPLPHYEPPAE
YVAHPPEEGWLTLISAAAHHFVSSSMANQPSLRAKEGEPTIEINPVDAAARNIRDGDVVVVSNQRGQCHLRAVVSTNVPP
GVVSAVKGHWPSLSPDGRNVNWTTPDALADLGGQSTFHSNRVQVWPSRI
>Mature_688_residues
TTQRVRGCCPHDCPDTCATITEVRDGRAVAFYADPEHPITQGWLCAKVRPYLERVYSPDRLLYPLRRVGPKGSGRWERIS
WDEAITTIAERWQAIIAEYGAAAILPYSYSGTLGLLQTAIVDARLWNRMGASGLDRTICCAAAHAAVYATLGARRSPDYD
DLRHSRLIILWGHNPASAGPHAMPFIRQAQRAGAYVVVIDPRRTATARSADLHLAIRPATDGALALGMMNVIFAENLHDE
AWLEAHTVGWRELRARAAEYDPERVATITGLPVSTIIELARRYATTKPAIIKTADGIQRHQNGGQTFRALLCLPAVVGQY
GVRGGGLAYSTGGYATWDAEAVGHASECPPVPRTVNMNRLGAALTGEVTDPPIMSLFVYCANPLASSPNAGLIEQGLRRE
DLFTVVHELFMTDTARYADIVLPATSQLEHLDLHRAYGHRYLTLNQPAIAPLGEAKSNWDVTRLLAQAMGYSEPWLHESA
EEAIRGVLDASRARNPFLAGITFERLQQEGTIRLTLSPENEVPFADGHFPTPSGKVELWSATMAAQGLDPLPHYEPPAEY
VAHPPEEGWLTLISAAAHHFVSSSMANQPSLRAKEGEPTIEINPVDAAARNIRDGDVVVVSNQRGQCHLRAVVSTNVPPG
VVSAVKGHWPSLSPDGRNVNWTTPDALADLGGQSTFHSNRVQVWPSRI

Specific function: Terminal Reductase During Anaerobic Growth On Various Sulfoxide And N-Oxide Compounds. Allows E.Coli To Grow Anaerobically On Me(2)So As Respiratory Oxidant. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=758, Percent_Identity=30.6068601583113, Blast_Score=252, Evalue=6e-68,
Organism=Escherichia coli, GI171474008, Length=756, Percent_Identity=29.1005291005291, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI1787870, Length=746, Percent_Identity=28.9544235924933, Blast_Score=228, Evalue=9e-61,
Organism=Escherichia coli, GI3868721, Length=685, Percent_Identity=26.7153284671533, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI87081994, Length=669, Percent_Identity=28.9985052316891, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI145693196, Length=682, Percent_Identity=26.6862170087977, Blast_Score=151, Evalue=2e-37,
Organism=Escherichia coli, GI1787231, Length=727, Percent_Identity=25.8596973865199, Blast_Score=147, Evalue=3e-36,
Organism=Escherichia coli, GI1788534, Length=796, Percent_Identity=22.8643216080402, Blast_Score=108, Evalue=2e-24,
Organism=Escherichia coli, GI3868720, Length=409, Percent_Identity=22.9828850855746, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI3868719, Length=406, Percent_Identity=21.9211822660099, Blast_Score=87, Evalue=5e-18,
Organism=Escherichia coli, GI1787778, Length=299, Percent_Identity=24.7491638795987, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 75207; Mature: 75076

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00490 MOLYBDOPTERIN_PROK_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQRVRGCCPHDCPDTCATITEVRDGRAVAFYADPEHPITQGWLCAKVRPYLERVYSPD
CCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCH
RLLYPLRRVGPKGSGRWERISWDEAITTIAERWQAIIAEYGAAAILPYSYSGTLGLLQTA
HHHHHHHHCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHH
IVDARLWNRMGASGLDRTICCAAAHAAVYATLGARRSPDYDDLRHSRLIILWGHNPASAG
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEEECCCCCCCC
PHAMPFIRQAQRAGAYVVVIDPRRTATARSADLHLAIRPATDGALALGMMNVIFAENLHD
CCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCC
EAWLEAHTVGWRELRARAAEYDPERVATITGLPVSTIIELARRYATTKPAIIKTADGIQR
HHHHHHHHCCHHHHHHHHHCCCHHHEEEECCCCHHHHHHHHHHHCCCCCCEEECCHHHHH
HQNGGQTFRALLCLPAVVGQYGVRGGGLAYSTGGYATWDAEAVGHASECPPVPRTVNMNR
HCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEECHHHCCCCCCCCCCCCCCCHHH
LGAALTGEVTDPPIMSLFVYCANPLASSPNAGLIEQGLRREDLFTVVHELFMTDTARYAD
HCCEEECCCCCCHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCEE
IVLPATSQLEHLDLHRAYGHRYLTLNQPAIAPLGEAKSNWDVTRLLAQAMGYSEPWLHES
EEECCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHH
AEEAIRGVLDASRARNPFLAGITFERLQQEGTIRLTLSPENEVPFADGHFPTPSGKVELW
HHHHHHHHHHHHHCCCCEEECCCHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEE
SATMAAQGLDPLPHYEPPAEYVAHPPEEGWLTLISAAAHHFVSSSMANQPSLRAKEGEPT
EHHHHHCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCE
IEINPVDAAARNIRDGDVVVVSNQRGQCHLRAVVSTNVPPGVVSAVKGHWPSLSPDGRNV
EEECCHHHHHHCCCCCCEEEEECCCCCEEEEEEEECCCCCHHHHHHHCCCCCCCCCCCCC
NWTTPDALADLGGQSTFHSNRVQVWPSRI
CCCCHHHHHHCCCCCCCCCCCEEECCCCC
>Mature Secondary Structure 
TTQRVRGCCPHDCPDTCATITEVRDGRAVAFYADPEHPITQGWLCAKVRPYLERVYSPD
CCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCH
RLLYPLRRVGPKGSGRWERISWDEAITTIAERWQAIIAEYGAAAILPYSYSGTLGLLQTA
HHHHHHHHCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHH
IVDARLWNRMGASGLDRTICCAAAHAAVYATLGARRSPDYDDLRHSRLIILWGHNPASAG
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEEECCCCCCCC
PHAMPFIRQAQRAGAYVVVIDPRRTATARSADLHLAIRPATDGALALGMMNVIFAENLHD
CCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCC
EAWLEAHTVGWRELRARAAEYDPERVATITGLPVSTIIELARRYATTKPAIIKTADGIQR
HHHHHHHHCCHHHHHHHHHCCCHHHEEEECCCCHHHHHHHHHHHCCCCCCEEECCHHHHH
HQNGGQTFRALLCLPAVVGQYGVRGGGLAYSTGGYATWDAEAVGHASECPPVPRTVNMNR
HCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEECHHHCCCCCCCCCCCCCCCHHH
LGAALTGEVTDPPIMSLFVYCANPLASSPNAGLIEQGLRREDLFTVVHELFMTDTARYAD
HCCEEECCCCCCHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCEE
IVLPATSQLEHLDLHRAYGHRYLTLNQPAIAPLGEAKSNWDVTRLLAQAMGYSEPWLHES
EEECCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHH
AEEAIRGVLDASRARNPFLAGITFERLQQEGTIRLTLSPENEVPFADGHFPTPSGKVELW
HHHHHHHHHHHHHCCCCEEECCCHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEE
SATMAAQGLDPLPHYEPPAEYVAHPPEEGWLTLISAAAHHFVSSSMANQPSLRAKEGEPT
EHHHHHCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCE
IEINPVDAAARNIRDGDVVVVSNQRGQCHLRAVVSTNVPPGVVSAVKGHWPSLSPDGRNV
EEECCHHHHHHCCCCCCEEEEECCCCCEEEEEEEECCCCCHHHHHHHCCCCCCCCCCCCC
NWTTPDALADLGGQSTFHSNRVQVWPSRI
CCCCHHHHHHCCCCCCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]