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The map label for this gene is dapE [H]

Identifier: 222524381

GI number: 222524381

Start: 1399357

End: 1400688

Strand: Direct

Name: dapE [H]

Synonym: Chy400_1105

Alternate gene names: 222524381

Gene position: 1399357-1400688 (Clockwise)

Preceding gene: 222524377

Following gene: 222524385

Centisome position: 26.56

GC content: 57.28

Gene sequence:

>1332_bases
GTGACTGTCCATGCACCGCCGTCAATTGAGCAGCTTGTTCAGATCTTGCGGTACCTCTGCAAGCAGCCATCGACTGCCGG
TCAGATCGATGAGTTGCGCACAATGGCTGAACATATCGCCCTTCTTGTGCGTCGGTTAGGCATGCATGTGCGCATTGCGG
CTACCGATACCGCACCGGTGATTATTGCTCATCGGTCAGGACGACGGCCACAGACCCTTTTGCTCTATCATCACTACGAT
ACGCCACCAACCGGCCCGTGGCGACACTGGTCACACGAACCGTTTGATATTGCTGAACGTGATGGTCGTGTATTTGGACG
TGGTGTAGCCGGTGGCAAAGGTGCCCTGGCAGCGCATCTGGCAGCCCTGCAGACCATTCTGCACCGTGAAGGGGAATTGC
CGTGTGGGATCACCCTGGTCATTGAAGGGGCAGCTACAACGGGTAGTTCTGGTCTGCAAGAAGCCCTGCACGCGCATAGT
GATCTGCTGCAATGTGACGCGATCCTGGCCAGTGTTGGTGACCGCGATGCTACCGGTACGCCTATCTGCGTGAGTGGTAG
TAAAGGCTGGATCCGTCTGCGTCTGAGTGCGCGTGGGCCGGCGTATCCCTTGCCAACCGGCTTTGCCGCCAGCGTTGCCA
ATCCGCTCTGGCGGCTGATCTGGGCCCTGGCTTCTCTCAAGGGTGATGATGAAGATGTGCGGATCGAGGGGTTTTACGAC
GCGGTTGAAGGTCCGTCGCGCAATGATAATGCGGTGATCCGACAACTGCAACTGGCCTCGGCTGCACGACGGGATGCCTG
GCGGATCGATCAGTTTCTTTTCGGTATCGACGGTGCAGCGTTGAGTCGCTCGGAAGTGACGCTCCCCACCTGTAATGTGA
GTAGCATCACCGTTGAACCATCCGGTGAGACACCCGTTATCCCTGCACAGGCGACGGCGCTACTCGATATTCAGTTGGTA
CCCCATCAACAGCCGGATGCGATTGTGGCATTGATACAGCACCACCTGCACGAGAAGGGGTTTCCTGACATTGTGATTGA
GCGGTTGCCTGGCGGGTATCCCCCGTTAATGACTGCGACCGATAATCCCTTCGTGACGCAGGTGACTGCTATCGGCGCAA
CCGTATTTGAACGGCCACCGGTGGTTGTGCCGGCTGGCCCATACACGGTGCCTTTCAGCTTACTGGTTGGCCATCACCCA
ATCCCAACCGTGAGCCTTGGCCTGACACCGGTTGTCAGCGCGACCTTTGCTCCCGATGAATACATTCCGATCACCGACCT
GAGTCGTCATAGTCGTTTGCTGATCGAATTACTCGACCAGTTGTGGCGGTAA

Upstream 100 bases:

>100_bases
TCGCACTTCATTCTAGCATGACCCTCGCGTAGCGTGCAGTGAACACCGTGTTATAATTCGTTCACTAAACGCAAATGTGT
GAGCAAGAGGGGGCATCTTG

Downstream 100 bases:

>100_bases
GTACTTCCAGGAGCACGGTCACATTCTACTGCGTCGCTGCCGGTATATTAAACGTAGGCACCCCACTGCATAACAATCCC
ATTTCGGTAATACACGTTCC

Product: peptidase M20

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MTVHAPPSIEQLVQILRYLCKQPSTAGQIDELRTMAEHIALLVRRLGMHVRIAATDTAPVIIAHRSGRRPQTLLLYHHYD
TPPTGPWRHWSHEPFDIAERDGRVFGRGVAGGKGALAAHLAALQTILHREGELPCGITLVIEGAATTGSSGLQEALHAHS
DLLQCDAILASVGDRDATGTPICVSGSKGWIRLRLSARGPAYPLPTGFAASVANPLWRLIWALASLKGDDEDVRIEGFYD
AVEGPSRNDNAVIRQLQLASAARRDAWRIDQFLFGIDGAALSRSEVTLPTCNVSSITVEPSGETPVIPAQATALLDIQLV
PHQQPDAIVALIQHHLHEKGFPDIVIERLPGGYPPLMTATDNPFVTQVTAIGATVFERPPVVVPAGPYTVPFSLLVGHHP
IPTVSLGLTPVVSATFAPDEYIPITDLSRHSRLLIELLDQLWR

Sequences:

>Translated_443_residues
MTVHAPPSIEQLVQILRYLCKQPSTAGQIDELRTMAEHIALLVRRLGMHVRIAATDTAPVIIAHRSGRRPQTLLLYHHYD
TPPTGPWRHWSHEPFDIAERDGRVFGRGVAGGKGALAAHLAALQTILHREGELPCGITLVIEGAATTGSSGLQEALHAHS
DLLQCDAILASVGDRDATGTPICVSGSKGWIRLRLSARGPAYPLPTGFAASVANPLWRLIWALASLKGDDEDVRIEGFYD
AVEGPSRNDNAVIRQLQLASAARRDAWRIDQFLFGIDGAALSRSEVTLPTCNVSSITVEPSGETPVIPAQATALLDIQLV
PHQQPDAIVALIQHHLHEKGFPDIVIERLPGGYPPLMTATDNPFVTQVTAIGATVFERPPVVVPAGPYTVPFSLLVGHHP
IPTVSLGLTPVVSATFAPDEYIPITDLSRHSRLLIELLDQLWR
>Mature_442_residues
TVHAPPSIEQLVQILRYLCKQPSTAGQIDELRTMAEHIALLVRRLGMHVRIAATDTAPVIIAHRSGRRPQTLLLYHHYDT
PPTGPWRHWSHEPFDIAERDGRVFGRGVAGGKGALAAHLAALQTILHREGELPCGITLVIEGAATTGSSGLQEALHAHSD
LLQCDAILASVGDRDATGTPICVSGSKGWIRLRLSARGPAYPLPTGFAASVANPLWRLIWALASLKGDDEDVRIEGFYDA
VEGPSRNDNAVIRQLQLASAARRDAWRIDQFLFGIDGAALSRSEVTLPTCNVSSITVEPSGETPVIPAQATALLDIQLVP
HQQPDAIVALIQHHLHEKGFPDIVIERLPGGYPPLMTATDNPFVTQVTAIGATVFERPPVVVPAGPYTVPFSLLVGHHPI
PTVSLGLTPVVSATFAPDEYIPITDLSRHSRLLIELLDQLWR

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI21071039, Length=410, Percent_Identity=23.9024390243902, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI271398239, Length=364, Percent_Identity=24.4505494505494, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17563146, Length=412, Percent_Identity=25, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI193211059, Length=274, Percent_Identity=24.0875912408759, Blast_Score=86, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6319758, Length=351, Percent_Identity=21.6524216524217, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6321159, Length=290, Percent_Identity=24.8275862068966, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24585880, Length=327, Percent_Identity=24.1590214067278, Blast_Score=99, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 47933; Mature: 47802

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVHAPPSIEQLVQILRYLCKQPSTAGQIDELRTMAEHIALLVRRLGMHVRIAATDTAPV
CCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCE
IIAHRSGRRPQTLLLYHHYDTPPTGPWRHWSHEPFDIAERDGRVFGRGVAGGKGALAAHL
EEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHCCCCCEEECCCCCCCHHHHHHH
AALQTILHREGELPCGITLVIEGAATTGSSGLQEALHAHSDLLQCDAILASVGDRDATGT
HHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PICVSGSKGWIRLRLSARGPAYPLPTGFAASVANPLWRLIWALASLKGDDEDVRIEGFYD
CEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEH
AVEGPSRNDNAVIRQLQLASAARRDAWRIDQFLFGIDGAALSRSEVTLPTCNVSSITVEP
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEEECC
SGETPVIPAQATALLDIQLVPHQQPDAIVALIQHHLHEKGFPDIVIERLPGGYPPLMTAT
CCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEECC
DNPFVTQVTAIGATVFERPPVVVPAGPYTVPFSLLVGHHPIPTVSLGLTPVVSATFAPDE
CCCCEEEHHHHHHHHHCCCCEEECCCCCCCCHHHHCCCCCCCEEECCCCHHHEEECCCCC
YIPITDLSRHSRLLIELLDQLWR
CCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TVHAPPSIEQLVQILRYLCKQPSTAGQIDELRTMAEHIALLVRRLGMHVRIAATDTAPV
CCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCE
IIAHRSGRRPQTLLLYHHYDTPPTGPWRHWSHEPFDIAERDGRVFGRGVAGGKGALAAHL
EEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHCCCCCEEECCCCCCCHHHHHHH
AALQTILHREGELPCGITLVIEGAATTGSSGLQEALHAHSDLLQCDAILASVGDRDATGT
HHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PICVSGSKGWIRLRLSARGPAYPLPTGFAASVANPLWRLIWALASLKGDDEDVRIEGFYD
CEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEH
AVEGPSRNDNAVIRQLQLASAARRDAWRIDQFLFGIDGAALSRSEVTLPTCNVSSITVEP
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEEECC
SGETPVIPAQATALLDIQLVPHQQPDAIVALIQHHLHEKGFPDIVIERLPGGYPPLMTAT
CCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEECC
DNPFVTQVTAIGATVFERPPVVVPAGPYTVPFSLLVGHHPIPTVSLGLTPVVSATFAPDE
CCCCEEEHHHHHHHHHCCCCEEECCCCCCCCHHHHCCCCCCCEEECCCCHHHEEECCCCC
YIPITDLSRHSRLLIELLDQLWR
CCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA