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The map label for this gene is rnjA [H]

Identifier: 222524357

GI number: 222524357

Start: 1373279

End: 1374949

Strand: Direct

Name: rnjA [H]

Synonym: Chy400_1080

Alternate gene names: 222524357

Gene position: 1373279-1374949 (Clockwise)

Preceding gene: 222524356

Following gene: 222524362

Centisome position: 26.06

GC content: 57.27

Gene sequence:

>1671_bases
ATGGCCAAACAACGTCTCCGTGTGATTCCACTCGGTGGCGCCGGGGAGGTCGGTCGGAACATGTGGGTGGTCGAGTATGG
CGATGACATCGTCATTCTTGACTGTGGTGTCATGTTTCCCGATGCCGATATGCTCGGTGTTGATCTCGTGTTGCCCGACA
TTACCTACTTACGCGAGAAGACGGATCATATTCGCGGTATTCTCCTCACCCACGGTCACGAAGACCATATCGGCGCCGTG
CCACATCTGATCGCCGATTTAGGTTTTCCACCGATCTACGGTACCAAACTGACCCTGGGCCTGCTTGGCAATCGGTTGCG
CGAACATCGTTTGCACGATAAGGTGACGACCGTTCAAATCAATGAACGGGTGCCGTTTCAGGTTGGTCAGTTCAATGTCG
AAGCCTTCCACATTGCCCACTCCATCCCCGATGCAGTCGGGTTTGCCATTACCACACCTGCCGGTCTGCTGGTCTACCTG
ACCGACTGGAAGCTCGATCACACGCCGGTTGATGGCCGACCAACCGATCTGGAGAAGATTGCCGAACTTGGTAATCGCAA
GCCGCTGGTGCTGGTAACCGATTGTGTACGGGTTGAATCAAAAGGCTACACCCCCAGTGAAGCGACTGTTGGTGAAGCCT
TTGATAATATCTTCGCTACCGCACCGGGCCGGATCATTGTCGCAACCTTCGCCTCAAACATCAGTCGAGTGCAGCAGGTA
ATCGACTCGGCAGAGGCGTATGGCCGTAAAGTGATGCTGGCCGGGCGGAGTATGGAGACCAATGCCCGCATCGCCTTCGA
GTTAGGCTATTTGCGGGCCGATGAGAGCACCATCATCCGTCCGCACGAAATGTCGGCCTACCCCGACGAGGAGATTGCCA
TCGTCTGCACCGGTAGCCAGGGTGAGCCAATGTCGGCCCTTAACCGCATGGCCAACCGTGACTATCCGAATGTCAAGATC
AAACCCGGCGACACCGTAGTGCTCTCGGCTACGCCGATTCCCGGCAACGAGGTGAGTGTCAATAAGGTGATCAACAACCT
CTTCAAGCTGGGGGCTGATGTGATTTATGGCCCTGAAGCACGGGTCCACGTCTCCGGGCACGCCGCGCAGGAAGAGTTGA
AGCTGGTGCTGGCGTTGCTGCGCCCCAGTTACATTGTGCCCTTCCACGGCGACTATCGCCACCTGATGCTTTACCGCAAG
CTGGCGACAACAATTGGCGCGGTGCCCGACAGTGAGCGGGTGCTGCTGGCCGAAGGGGGCACGATTATGGAGTTCTCCCC
CGGCTTCGGTAAGATTACCGGCAAGGCCCCGGTTGGGTATATCTACGTTGATGGTACGACGGTTGGTGATGTGGGCAATG
TGGTTGTCCGTGATCGCCAGTTGCTGGCTAAAGACGGTATGCTGATCGTGGTGGTGAGCATTGATAGCGCCACCGGTCAG
CTCGTTGCCGGGCCAGACGTGGTCTCGCGTGGCTTCGTCTATATGCGCCAGAGCCAGGATTTGATCGAGGCCACCAAAGA
AACGGTGCGCGCTGCCCTGGCCAACCGCAATGGCAGCAACCCGGTGGACGCAGCCTACATCAATCGCAAAATTCGCGATG
TGGTGAGCGAATTCCTCTACCGCGAGACGCGGCGGCGGCCAATGGTACTGCCGATGGTTATGGAAGTGTGA

Upstream 100 bases:

>100_bases
CGTGCGTAAGGCGCCTGCGCTGGAGCGCCTTTGTAAGCGAATATCTGGATACAACAGAAAGGAGTAGGGATCGTCTGCAC
AACGATCCTGGTAGAAATCT

Downstream 100 bases:

>100_bases
TTGTGGTTGCGAGCGAGACGTTGTGGTTGCCACAACGTCTCGCGGCTTTACGGATCGATCCGCGGCAGCACGATCCCCGT
CTGTCCCTGATATTTACCCT

Product: beta-lactamase domain-containing protein

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MAKQRLRVIPLGGAGEVGRNMWVVEYGDDIVILDCGVMFPDADMLGVDLVLPDITYLREKTDHIRGILLTHGHEDHIGAV
PHLIADLGFPPIYGTKLTLGLLGNRLREHRLHDKVTTVQINERVPFQVGQFNVEAFHIAHSIPDAVGFAITTPAGLLVYL
TDWKLDHTPVDGRPTDLEKIAELGNRKPLVLVTDCVRVESKGYTPSEATVGEAFDNIFATAPGRIIVATFASNISRVQQV
IDSAEAYGRKVMLAGRSMETNARIAFELGYLRADESTIIRPHEMSAYPDEEIAIVCTGSQGEPMSALNRMANRDYPNVKI
KPGDTVVLSATPIPGNEVSVNKVINNLFKLGADVIYGPEARVHVSGHAAQEELKLVLALLRPSYIVPFHGDYRHLMLYRK
LATTIGAVPDSERVLLAEGGTIMEFSPGFGKITGKAPVGYIYVDGTTVGDVGNVVVRDRQLLAKDGMLIVVVSIDSATGQ
LVAGPDVVSRGFVYMRQSQDLIEATKETVRAALANRNGSNPVDAAYINRKIRDVVSEFLYRETRRRPMVLPMVMEV

Sequences:

>Translated_556_residues
MAKQRLRVIPLGGAGEVGRNMWVVEYGDDIVILDCGVMFPDADMLGVDLVLPDITYLREKTDHIRGILLTHGHEDHIGAV
PHLIADLGFPPIYGTKLTLGLLGNRLREHRLHDKVTTVQINERVPFQVGQFNVEAFHIAHSIPDAVGFAITTPAGLLVYL
TDWKLDHTPVDGRPTDLEKIAELGNRKPLVLVTDCVRVESKGYTPSEATVGEAFDNIFATAPGRIIVATFASNISRVQQV
IDSAEAYGRKVMLAGRSMETNARIAFELGYLRADESTIIRPHEMSAYPDEEIAIVCTGSQGEPMSALNRMANRDYPNVKI
KPGDTVVLSATPIPGNEVSVNKVINNLFKLGADVIYGPEARVHVSGHAAQEELKLVLALLRPSYIVPFHGDYRHLMLYRK
LATTIGAVPDSERVLLAEGGTIMEFSPGFGKITGKAPVGYIYVDGTTVGDVGNVVVRDRQLLAKDGMLIVVVSIDSATGQ
LVAGPDVVSRGFVYMRQSQDLIEATKETVRAALANRNGSNPVDAAYINRKIRDVVSEFLYRETRRRPMVLPMVMEV
>Mature_555_residues
AKQRLRVIPLGGAGEVGRNMWVVEYGDDIVILDCGVMFPDADMLGVDLVLPDITYLREKTDHIRGILLTHGHEDHIGAVP
HLIADLGFPPIYGTKLTLGLLGNRLREHRLHDKVTTVQINERVPFQVGQFNVEAFHIAHSIPDAVGFAITTPAGLLVYLT
DWKLDHTPVDGRPTDLEKIAELGNRKPLVLVTDCVRVESKGYTPSEATVGEAFDNIFATAPGRIIVATFASNISRVQQVI
DSAEAYGRKVMLAGRSMETNARIAFELGYLRADESTIIRPHEMSAYPDEEIAIVCTGSQGEPMSALNRMANRDYPNVKIK
PGDTVVLSATPIPGNEVSVNKVINNLFKLGADVIYGPEARVHVSGHAAQEELKLVLALLRPSYIVPFHGDYRHLMLYRKL
ATTIGAVPDSERVLLAEGGTIMEFSPGFGKITGKAPVGYIYVDGTTVGDVGNVVVRDRQLLAKDGMLIVVVSIDSATGQL
VAGPDVVSRGFVYMRQSQDLIEATKETVRAALANRNGSNPVDAAYINRKIRDVVSEFLYRETRRRPMVLPMVMEV

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 60889; Mature: 60758

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQRLRVIPLGGAGEVGRNMWVVEYGDDIVILDCGVMFPDADMLGVDLVLPDITYLREK
CCCCCEEEEEECCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCEEEEECCCHHHHHHH
TDHIRGILLTHGHEDHIGAVPHLIADLGFPPIYGTKLTLGLLGNRLREHRLHDKVTTVQI
HHHHEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHCCEEEEEE
NERVPFQVGQFNVEAFHIAHSIPDAVGFAITTPAGLLVYLTDWKLDHTPVDGRPTDLEKI
CCCCCEEECCEEEEEEEEEHHCCHHHCEEEECCCCEEEEEECCEECCCCCCCCCCCHHHH
AELGNRKPLVLVTDCVRVESKGYTPSEATVGEAFDNIFATAPGRIIVATFASNISRVQQV
HHHCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHH
IDSAEAYGRKVMLAGRSMETNARIAFELGYLRADESTIIRPHEMSAYPDEEIAIVCTGSQ
HHHHHHCCCEEEEECCCCCCCCEEEEEEEEEECCCCCEECCCCCCCCCCCCEEEEEECCC
GEPMSALNRMANRDYPNVKIKPGDTVVLSATPIPGNEVSVNKVINNLFKLGADVIYGPEA
CCHHHHHHHHHCCCCCCEEECCCCEEEEEECCCCCCCEEHHHHHHHHHHCCCCEEECCCE
RVHVSGHAAQEELKLVLALLRPSYIVPFHGDYRHLMLYRKLATTIGAVPDSERVLLAEGG
EEEECCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCC
TIMEFSPGFGKITGKAPVGYIYVDGTTVGDVGNVVVRDRQLLAKDGMLIVVVSIDSATGQ
EEEEECCCCCEEECCCCEEEEEECCCEECCCCCEEEECHHHHHCCCEEEEEEEECCCCCC
LVAGPDVVSRGFVYMRQSQDLIEATKETVRAALANRNGSNPVDAAYINRKIRDVVSEFLY
EEECCHHHHCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
RETRRRPMVLPMVMEV
HHHCCCCEEEHHHHCC
>Mature Secondary Structure 
AKQRLRVIPLGGAGEVGRNMWVVEYGDDIVILDCGVMFPDADMLGVDLVLPDITYLREK
CCCCEEEEEECCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCEEEEECCCHHHHHHH
TDHIRGILLTHGHEDHIGAVPHLIADLGFPPIYGTKLTLGLLGNRLREHRLHDKVTTVQI
HHHHEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHCCEEEEEE
NERVPFQVGQFNVEAFHIAHSIPDAVGFAITTPAGLLVYLTDWKLDHTPVDGRPTDLEKI
CCCCCEEECCEEEEEEEEEHHCCHHHCEEEECCCCEEEEEECCEECCCCCCCCCCCHHHH
AELGNRKPLVLVTDCVRVESKGYTPSEATVGEAFDNIFATAPGRIIVATFASNISRVQQV
HHHCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHH
IDSAEAYGRKVMLAGRSMETNARIAFELGYLRADESTIIRPHEMSAYPDEEIAIVCTGSQ
HHHHHHCCCEEEEECCCCCCCCEEEEEEEEEECCCCCEECCCCCCCCCCCCEEEEEECCC
GEPMSALNRMANRDYPNVKIKPGDTVVLSATPIPGNEVSVNKVINNLFKLGADVIYGPEA
CCHHHHHHHHHCCCCCCEEECCCCEEEEEECCCCCCCEEHHHHHHHHHHCCCCEEECCCE
RVHVSGHAAQEELKLVLALLRPSYIVPFHGDYRHLMLYRKLATTIGAVPDSERVLLAEGG
EEEECCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCC
TIMEFSPGFGKITGKAPVGYIYVDGTTVGDVGNVVVRDRQLLAKDGMLIVVVSIDSATGQ
EEEEECCCCCEEECCCCEEEEEECCCEECCCCCEEEECHHHHHCCCEEEEEEEECCCCCC
LVAGPDVVSRGFVYMRQSQDLIEATKETVRAALANRNGSNPVDAAYINRKIRDVVSEFLY
EEECCHHHHCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
RETRRRPMVLPMVMEV
HHHCCCCEEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]