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The map label for this gene is yomI [H]

Identifier: 222524349

GI number: 222524349

Start: 1363374

End: 1365710

Strand: Reverse

Name: yomI [H]

Synonym: Chy400_1072

Alternate gene names: 222524349

Gene position: 1365710-1363374 (Counterclockwise)

Preceding gene: 222524350

Following gene: 222524348

Centisome position: 25.92

GC content: 63.33

Gene sequence:

>2337_bases
ATGGCGTTGATGCAGTTGTCTCGACAGCGTATCGCAGCGCTGTTGACCTGCTGGCTTTGTATGTACCTGGTCGCCTGCGT
GGCACCCATGGCGCAATCATCGGCAACTGTGACCCCTGAACCATTACCGGCAACACCAACTGCCGTTCCAATCACTGCCA
CCGATCTGCTGCAACGAGCGCAGGCAGCGCTTGAGATTGGTGATGATGACACCGCTGCCGAATTGCTCAGCCAGTTGTTG
CAGGTTTTCCCGGCTGCGCCGGAGACAACACCGGCACGCCTGTTATTGGCCCGTTCGTTCGCCGACCGTGGGCGCTGGAC
ATCGGCGGCTGAGGTGCTGCGGCCATTGCTCGCCGTACCGGGCACACCGGCGTATGCGCCGGCCCTGTTTCTGACCGCGC
GCGCCCATGAGGCTGCCGGTATGCACGAGGCCGCCGTAGCCACGTATGCCCAATATGAAGCGCTGAACACCCCGCTGGCA
CCGTATGCAGCAATGCGGGCTGCTGCACAGTTGCAGGCGCTTAATCGTCTGGCAGATGCCGAGACCACCTATCTGCGGGC
CGCCGCCGGTGAAATGGCCGCCGGTCAGCGTGCCGCTGCTTACGAACGGGCGATGATGCTGGCCGTTGCGCAGGAACGGC
TACCCGATGCCATCGATTACGCCCGCAACATCCTGTCCTTCGCCACCCAGGCTGATTACCGGGCACGCCTGCTGGTCCAG
GCAGCCGATCTGGCTGCAACCGCCGGTGATCCAGCGACCGCCAATGCGTTGCGCCGTGAAGCACTGGCAGCCTTTGCCGG
GGCAGAGACGGTCAGCGCCGTAGATGCGCTCCGCGCTGCCGGCGACCTGCAGTTCGATCCGTTTGCGGCGGCTGCGGCCT
ATCGCGCCGTTGAACGCTGGAATGACGTGATTGTCATGCTCGATATTGCCCTGGCGCGTGAGCAGAATCCCGGCGAAGCG
CTGCGCCAGCGCGGGTTGGCCCGGCGTGCGTTGGGGGATTTCGCGGGGGCATTGGCTGACCTGGCAGCGGCTCGCGAGCG
TGAGCCGGACAGTGATACCGCTCGCCAGGCCGCGCTGGACTGGATTCAAACCTACGGGCAGAGCGGGGCCACCGCCGAAG
CAGCAGCGCTCTACCGTCAGTATGCGGATGATCAGCCGGATGATCCGCGAGCGCCGATTGCCCTCGACCGGGCGGCTCAG
TTGTACGACCGGCTTGGCGATAGCGCAGCGGCAACCGCTACCCGGCTCGAACTCGGCCAACGGTATCCGACAACAACGGT
TGGCCTGACCGCGTTGCATCGGATTGCCCTGGCACGTTTCGATGCCGGTGATCTGGCCGGTGCCGGCGAACTCTGGCGGT
TGCTGGCAGAGCGGGGAGAAGGGATCGGACAGGCGTTAGGTGCATTCTGGGCCGGACGAGTGGCGAAACAGATGGGTGAT
GAGGCGGCTACCTCCTTCTTCCAACAGGCAATTCAGGCTGCACCGGAAAGTTACTATGCAGTGCGTGCGGCTGAAGAATC
AGGCACAATCACAGCAGGATCAATCCCTATCGCTGCACCCATTAGTAACGACGATTGGGCATTGCTGAGCGATTGGGTGC
TGAGCTGGGCAAGTGGTGAGACCGATCCGGGTCTGGTTGGCGTCGCTGAACGGGCACGGCTCCTGCGCGAGGTGGGACTG
TACACTGAAGCGCACGGCGAATGGCTCGATGGCCTGCGCCGGGCCGGCGACTCGCCGCTGAATCTGCTGGCGCTGGCGCA
GGCCGCCTATCAGGCCGGTGCCACCTACCCCGCATTACTGGCTGCGGAACGCATCAATCGCCTTGCGCCGGCCACTGCCG
CGCCGATACCGACAGCATTACTCCGCCTGCGCTTCCCGACACCGTATGCCGATGTGGTACAGCGTGAAGCAACTGCATTC
GGGGTTGATCCATTCTTACTCTACGCCCTCATCCGGCAAGAGAGCCTCTTTCAGCCGAACGCAACCTCGTGGGTAGGCGC
ACGCGGATTAACCCAGGTGATGCCCGATACCGGGCGCGGGATTGCTCAAAACCTGGGGGTCAGTGACTTCAACCTTGACG
ACCTGTATCGACCGCATGTCAGCATTCGCTTCGGCGCCTTCTACCTGGGTCGCCGGATCAGCGACATGAACGGCAGCCTG
CACGGTGCGCTGGCCGCCTACAACGGTGGACTCGGCAATGCACAGCGCTGGGCAAATGGCACTGTCGTCGGTGACCCGGA
TCGCTTTGTGGAGAGCATCGACTTTGCGGAAACGCGGAATTACGTCTGGGCAGTCTATGCGTTTTACGGCGTCTACCGCG
GGTTGTACGGGGAGTGA

Upstream 100 bases:

>100_bases
CCACGAATCGCGTGAGGAGTGCTCGCTCACCCACTCACCCTGCCGCCCGGCCTGACACACCCAGAAATGCTATACTGCCT
CTGAGTAGAAAGGAGCACGT

Downstream 100 bases:

>100_bases
GGTGAGAGGGCAATCCCACCCCGGCCCGCCCCCGCTGGCGGTGGGATTGCCCCACCCTGGCCTGCCCACGTTTAGAGGCA
GCTAGCATCTCCATAGTACT

Product: lytic transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 778; Mature: 777

Protein sequence:

>778_residues
MALMQLSRQRIAALLTCWLCMYLVACVAPMAQSSATVTPEPLPATPTAVPITATDLLQRAQAALEIGDDDTAAELLSQLL
QVFPAAPETTPARLLLARSFADRGRWTSAAEVLRPLLAVPGTPAYAPALFLTARAHEAAGMHEAAVATYAQYEALNTPLA
PYAAMRAAAQLQALNRLADAETTYLRAAAGEMAAGQRAAAYERAMMLAVAQERLPDAIDYARNILSFATQADYRARLLVQ
AADLAATAGDPATANALRREALAAFAGAETVSAVDALRAAGDLQFDPFAAAAAYRAVERWNDVIVMLDIALAREQNPGEA
LRQRGLARRALGDFAGALADLAAAREREPDSDTARQAALDWIQTYGQSGATAEAAALYRQYADDQPDDPRAPIALDRAAQ
LYDRLGDSAAATATRLELGQRYPTTTVGLTALHRIALARFDAGDLAGAGELWRLLAERGEGIGQALGAFWAGRVAKQMGD
EAATSFFQQAIQAAPESYYAVRAAEESGTITAGSIPIAAPISNDDWALLSDWVLSWASGETDPGLVGVAERARLLREVGL
YTEAHGEWLDGLRRAGDSPLNLLALAQAAYQAGATYPALLAAERINRLAPATAAPIPTALLRLRFPTPYADVVQREATAF
GVDPFLLYALIRQESLFQPNATSWVGARGLTQVMPDTGRGIAQNLGVSDFNLDDLYRPHVSIRFGAFYLGRRISDMNGSL
HGALAAYNGGLGNAQRWANGTVVGDPDRFVESIDFAETRNYVWAVYAFYGVYRGLYGE

Sequences:

>Translated_778_residues
MALMQLSRQRIAALLTCWLCMYLVACVAPMAQSSATVTPEPLPATPTAVPITATDLLQRAQAALEIGDDDTAAELLSQLL
QVFPAAPETTPARLLLARSFADRGRWTSAAEVLRPLLAVPGTPAYAPALFLTARAHEAAGMHEAAVATYAQYEALNTPLA
PYAAMRAAAQLQALNRLADAETTYLRAAAGEMAAGQRAAAYERAMMLAVAQERLPDAIDYARNILSFATQADYRARLLVQ
AADLAATAGDPATANALRREALAAFAGAETVSAVDALRAAGDLQFDPFAAAAAYRAVERWNDVIVMLDIALAREQNPGEA
LRQRGLARRALGDFAGALADLAAAREREPDSDTARQAALDWIQTYGQSGATAEAAALYRQYADDQPDDPRAPIALDRAAQ
LYDRLGDSAAATATRLELGQRYPTTTVGLTALHRIALARFDAGDLAGAGELWRLLAERGEGIGQALGAFWAGRVAKQMGD
EAATSFFQQAIQAAPESYYAVRAAEESGTITAGSIPIAAPISNDDWALLSDWVLSWASGETDPGLVGVAERARLLREVGL
YTEAHGEWLDGLRRAGDSPLNLLALAQAAYQAGATYPALLAAERINRLAPATAAPIPTALLRLRFPTPYADVVQREATAF
GVDPFLLYALIRQESLFQPNATSWVGARGLTQVMPDTGRGIAQNLGVSDFNLDDLYRPHVSIRFGAFYLGRRISDMNGSL
HGALAAYNGGLGNAQRWANGTVVGDPDRFVESIDFAETRNYVWAVYAFYGVYRGLYGE
>Mature_777_residues
ALMQLSRQRIAALLTCWLCMYLVACVAPMAQSSATVTPEPLPATPTAVPITATDLLQRAQAALEIGDDDTAAELLSQLLQ
VFPAAPETTPARLLLARSFADRGRWTSAAEVLRPLLAVPGTPAYAPALFLTARAHEAAGMHEAAVATYAQYEALNTPLAP
YAAMRAAAQLQALNRLADAETTYLRAAAGEMAAGQRAAAYERAMMLAVAQERLPDAIDYARNILSFATQADYRARLLVQA
ADLAATAGDPATANALRREALAAFAGAETVSAVDALRAAGDLQFDPFAAAAAYRAVERWNDVIVMLDIALAREQNPGEAL
RQRGLARRALGDFAGALADLAAAREREPDSDTARQAALDWIQTYGQSGATAEAAALYRQYADDQPDDPRAPIALDRAAQL
YDRLGDSAAATATRLELGQRYPTTTVGLTALHRIALARFDAGDLAGAGELWRLLAERGEGIGQALGAFWAGRVAKQMGDE
AATSFFQQAIQAAPESYYAVRAAEESGTITAGSIPIAAPISNDDWALLSDWVLSWASGETDPGLVGVAERARLLREVGLY
TEAHGEWLDGLRRAGDSPLNLLALAQAAYQAGATYPALLAAERINRLAPATAAPIPTALLRLRFPTPYADVVQREATAFG
VDPFLLYALIRQESLFQPNATSWVGARGLTQVMPDTGRGIAQNLGVSDFNLDDLYRPHVSIRFGAFYLGRRISDMNGSLH
GALAAYNGGLGNAQRWANGTVVGDPDRFVESIDFAETRNYVWAVYAFYGVYRGLYGE

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 10 TPR repeats [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=161, Percent_Identity=34.1614906832298, Blast_Score=79, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR008258
- InterPro:   IPR016047
- InterPro:   IPR010090
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01551 Peptidase_M23; PF10145 PhageMin_Tail; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 83078; Mature: 82947

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALMQLSRQRIAALLTCWLCMYLVACVAPMAQSSATVTPEPLPATPTAVPITATDLLQRA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
QAALEIGDDDTAAELLSQLLQVFPAAPETTPARLLLARSFADRGRWTSAAEVLRPLLAVP
HHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
GTPAYAPALFLTARAHEAAGMHEAAVATYAQYEALNTPLAPYAAMRAAAQLQALNRLADA
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ETTYLRAAAGEMAAGQRAAAYERAMMLAVAQERLPDAIDYARNILSFATQADYRARLLVQ
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHH
AADLAATAGDPATANALRREALAAFAGAETVSAVDALRAAGDLQFDPFAAAAAYRAVERW
HHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
NDVIVMLDIALAREQNPGEALRQRGLARRALGDFAGALADLAAAREREPDSDTARQAALD
CCEEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
WIQTYGQSGATAEAAALYRQYADDQPDDPRAPIALDRAAQLYDRLGDSAAATATRLELGQ
HHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCC
RYPTTTVGLTALHRIALARFDAGDLAGAGELWRLLAERGEGIGQALGAFWAGRVAKQMGD
CCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCH
EAATSFFQQAIQAAPESYYAVRAAEESGTITAGSIPIAAPISNDDWALLSDWVLSWASGE
HHHHHHHHHHHHHCCCHHEEEEEECCCCCEEECCCEEEECCCCCCHHHHHHHHHHHCCCC
TDPGLVGVAERARLLREVGLYTEAHGEWLDGLRRAGDSPLNLLALAQAAYQAGATYPALL
CCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHH
AAERINRLAPATAAPIPTALLRLRFPTPYADVVQREATAFGVDPFLLYALIRQESLFQPN
HHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
ATSWVGARGLTQVMPDTGRGIAQNLGVSDFNLDDLYRPHVSIRFGAFYLGRRISDMNGSL
CCCHHCCCCHHHHCCCCCCHHHHHCCCCCCCHHHHCCCCCEEEEHHHHHHHHHHHCCCCH
HGALAAYNGGLGNAQRWANGTVVGDPDRFVESIDFAETRNYVWAVYAFYGVYRGLYGE
HHHHHHHCCCCCCCHHHCCCCEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ALMQLSRQRIAALLTCWLCMYLVACVAPMAQSSATVTPEPLPATPTAVPITATDLLQRA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
QAALEIGDDDTAAELLSQLLQVFPAAPETTPARLLLARSFADRGRWTSAAEVLRPLLAVP
HHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
GTPAYAPALFLTARAHEAAGMHEAAVATYAQYEALNTPLAPYAAMRAAAQLQALNRLADA
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ETTYLRAAAGEMAAGQRAAAYERAMMLAVAQERLPDAIDYARNILSFATQADYRARLLVQ
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHH
AADLAATAGDPATANALRREALAAFAGAETVSAVDALRAAGDLQFDPFAAAAAYRAVERW
HHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
NDVIVMLDIALAREQNPGEALRQRGLARRALGDFAGALADLAAAREREPDSDTARQAALD
CCEEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
WIQTYGQSGATAEAAALYRQYADDQPDDPRAPIALDRAAQLYDRLGDSAAATATRLELGQ
HHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCC
RYPTTTVGLTALHRIALARFDAGDLAGAGELWRLLAERGEGIGQALGAFWAGRVAKQMGD
CCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCH
EAATSFFQQAIQAAPESYYAVRAAEESGTITAGSIPIAAPISNDDWALLSDWVLSWASGE
HHHHHHHHHHHHHCCCHHEEEEEECCCCCEEECCCEEEECCCCCCHHHHHHHHHHHCCCC
TDPGLVGVAERARLLREVGLYTEAHGEWLDGLRRAGDSPLNLLALAQAAYQAGATYPALL
CCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHH
AAERINRLAPATAAPIPTALLRLRFPTPYADVVQREATAFGVDPFLLYALIRQESLFQPN
HHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
ATSWVGARGLTQVMPDTGRGIAQNLGVSDFNLDDLYRPHVSIRFGAFYLGRRISDMNGSL
CCCHHCCCCHHHHCCCCCCHHHHHCCCCCCCHHHHCCCCCEEEEHHHHHHHHHHHCCCCH
HGALAAYNGGLGNAQRWANGTVVGDPDRFVESIDFAETRNYVWAVYAFYGVYRGLYGE
HHHHHHHCCCCCCCHHHCCCCEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]