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The map label for this gene is dnaC [H]

Identifier: 222524314

GI number: 222524314

Start: 1319051

End: 1320406

Strand: Direct

Name: dnaC [H]

Synonym: Chy400_1037

Alternate gene names: 222524314

Gene position: 1319051-1320406 (Clockwise)

Preceding gene: 222524313

Following gene: 222524315

Centisome position: 25.03

GC content: 56.49

Gene sequence:

>1356_bases
ATGGTGGAAAAGCGGGAAGATCGCCTCGAACGCAGTGTACCATTCGATCTCCAGGCTGAGCGGGCAACGCTTGGTTCCAT
CTTGCTTGAACGTGATGCAATCATTGCGGTTGCCGGCTGGCTTCCTGCCGAGTATTTTTACCTTGAAAAGCATGCCTTGA
TTTACGAGGCGATGCTGGCCTGTTACAACCGACGTGAACCGGCCGATCTGGCTACAGTCGCGGCGGAACTGCGTCGGCGT
GGGCAGCTCGAACTGGTGGGCGGGTTGAGTATGTTGAGCGAGCTGGTCACCGAGGTGCCAACTGCCGTTCACATCGAATA
CTACGCCCGCATTGTAGAACGGACGGCACTGCTCCGCCGCCTGATCGAGGCCGGTGGCCGGATTGCGGCCATGGGGTACG
ATGAAGCCAGTGATCTCGAAGAGACGCTGGATCGGGCCGAAGCTGAGTTGTTTGCCGTTTCACAGCGGCGGAACAATCAA
GATTTTGTCCATATTGGCCGTGTCGTCAATACCCTCTTTTCCCAAATAGAGTCGATGCAGGAGCGGCGAGGCGAGGTGAT
CGGAGTACCAACCGGTTACCACGACCTCGATGAGCTGACCGGTGGTTTGCAGCCGAGCGATCTGATCATCCTCGCCGCAC
GTCCGAGCGTGGGTAAAACCTCTCTCGCCCTCTCGCTGGCCTATAATGTGGCGTTTCACGCAAACGGAACTGTTGCCATC
TTCAGCCTTGAAATGAGCCGCGAACAGTTAGTTCAGCGTATGCTGGCGATGCATACCGGGATTGATATGCAGCGCCTGCG
CACCGGCAACCTACGCGGCGAAGAGCTGAGTCTGGCGATTGAAGGGTTGGGCGTGCTGTCAGAATTGCCGATCTATATTG
AAGATACTCCCGGCCTGAGCATCACCGATGTGCGGGCACGTGCCCGACGCCTGCACAGCGAGGTTGGTATCACACTTGTC
ATGATCGACTACCTGCAACTCATGTCGGGGCGACGCACCGATAATCGGGTGCAAGAAGTGAGTGACATCAGTCGAGGACT
GAAAGCCCTTGCCCGTGAACTCAACGTACCGGTGATTGCCCTTTCCCAGCTCTCGCGTGCGGTTGAGGGGCGGCAAAATC
ATGTACCGATGCTGAGCGACCTCCGCGAGTCGGGTTCAATTGAGCAGGACGCGGATATTGTGATGTTTATTTATCGTGAA
GAGTTGTACGATAAGGAGACCGACAAGAAAGGGATCGCCGAGATTCACATCGCCAAGCACCGTAATGGCCCGCTAGGCGT
GATCCCATTGCGGTTTGAGTCGCGGACAACGCGCTTTCAAAATCTCGAACGGTATCAGGCGCCGGAGGGTTTTTAG

Upstream 100 bases:

>100_bases
GTCGCTCGCTCAACTCAGTTCCTTGCCAATGTGCCGCAAATTGCGGCACATTGGCGTTTTATACCATTGATGTGGATAAC
CGGTGGGCAACGGGGGAAAC

Downstream 100 bases:

>100_bases
AGAAGACCGGGTACGCCAGCCCACATTGCGGTGCAGTTCGTGGCAAGCGTACCAGTGCTGTGACCTATGGACGTACACGT
GTGACAGACACATACCCGCT

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MVEKREDRLERSVPFDLQAERATLGSILLERDAIIAVAGWLPAEYFYLEKHALIYEAMLACYNRREPADLATVAAELRRR
GQLELVGGLSMLSELVTEVPTAVHIEYYARIVERTALLRRLIEAGGRIAAMGYDEASDLEETLDRAEAELFAVSQRRNNQ
DFVHIGRVVNTLFSQIESMQERRGEVIGVPTGYHDLDELTGGLQPSDLIILAARPSVGKTSLALSLAYNVAFHANGTVAI
FSLEMSREQLVQRMLAMHTGIDMQRLRTGNLRGEELSLAIEGLGVLSELPIYIEDTPGLSITDVRARARRLHSEVGITLV
MIDYLQLMSGRRTDNRVQEVSDISRGLKALARELNVPVIALSQLSRAVEGRQNHVPMLSDLRESGSIEQDADIVMFIYRE
ELYDKETDKKGIAEIHIAKHRNGPLGVIPLRFESRTTRFQNLERYQAPEGF

Sequences:

>Translated_451_residues
MVEKREDRLERSVPFDLQAERATLGSILLERDAIIAVAGWLPAEYFYLEKHALIYEAMLACYNRREPADLATVAAELRRR
GQLELVGGLSMLSELVTEVPTAVHIEYYARIVERTALLRRLIEAGGRIAAMGYDEASDLEETLDRAEAELFAVSQRRNNQ
DFVHIGRVVNTLFSQIESMQERRGEVIGVPTGYHDLDELTGGLQPSDLIILAARPSVGKTSLALSLAYNVAFHANGTVAI
FSLEMSREQLVQRMLAMHTGIDMQRLRTGNLRGEELSLAIEGLGVLSELPIYIEDTPGLSITDVRARARRLHSEVGITLV
MIDYLQLMSGRRTDNRVQEVSDISRGLKALARELNVPVIALSQLSRAVEGRQNHVPMLSDLRESGSIEQDADIVMFIYRE
ELYDKETDKKGIAEIHIAKHRNGPLGVIPLRFESRTTRFQNLERYQAPEGF
>Mature_451_residues
MVEKREDRLERSVPFDLQAERATLGSILLERDAIIAVAGWLPAEYFYLEKHALIYEAMLACYNRREPADLATVAAELRRR
GQLELVGGLSMLSELVTEVPTAVHIEYYARIVERTALLRRLIEAGGRIAAMGYDEASDLEETLDRAEAELFAVSQRRNNQ
DFVHIGRVVNTLFSQIESMQERRGEVIGVPTGYHDLDELTGGLQPSDLIILAARPSVGKTSLALSLAYNVAFHANGTVAI
FSLEMSREQLVQRMLAMHTGIDMQRLRTGNLRGEELSLAIEGLGVLSELPIYIEDTPGLSITDVRARARRLHSEVGITLV
MIDYLQLMSGRRTDNRVQEVSDISRGLKALARELNVPVIALSQLSRAVEGRQNHVPMLSDLRESGSIEQDADIVMFIYRE
ELYDKETDKKGIAEIHIAKHRNGPLGVIPLRFESRTTRFQNLERYQAPEGF

Specific function: Participates In Initiation And Elongation During Chromosome Replication; It Exhibits DNA-Dependent ATPase Activity And Contains Distinct Active Sites For ATP Binding, DNA Binding, And Interaction With DNAc Protein, Primase, And Other Prepriming Proteins.

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=443, Percent_Identity=45.823927765237, Blast_Score=356, Evalue=2e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 50637; Mature: 50637

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEKREDRLERSVPFDLQAERATLGSILLERDAIIAVAGWLPAEYFYLEKHALIYEAMLA
CCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
CYNRREPADLATVAAELRRRGQLELVGGLSMLSELVTEVPTAVHIEYYARIVERTALLRR
HHCCCCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LIEAGGRIAAMGYDEASDLEETLDRAEAELFAVSQRRNNQDFVHIGRVVNTLFSQIESMQ
HHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ERRGEVIGVPTGYHDLDELTGGLQPSDLIILAARPSVGKTSLALSLAYNVAFHANGTVAI
HHCCCEEECCCCCCCHHHHCCCCCCCCEEEEEECCCCCCHHHEEEEEEEEEEECCCEEEE
FSLEMSREQLVQRMLAMHTGIDMQRLRTGNLRGEELSLAIEGLGVLSELPIYIEDTPGLS
EEECCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHEEHHHHHHHHHHCCEEEECCCCCC
ITDVRARARRLHSEVGITLVMIDYLQLMSGRRTDNRVQEVSDISRGLKALARELNVPVIA
CHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
LSQLSRAVEGRQNHVPMLSDLRESGSIEQDADIVMFIYREELYDKETDKKGIAEIHIAKH
HHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCEEEEEEHHHHHCCCCCCCCCEEEEEEEC
RNGPLGVIPLRFESRTTRFQNLERYQAPEGF
CCCCEEEEEEEECCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MVEKREDRLERSVPFDLQAERATLGSILLERDAIIAVAGWLPAEYFYLEKHALIYEAMLA
CCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
CYNRREPADLATVAAELRRRGQLELVGGLSMLSELVTEVPTAVHIEYYARIVERTALLRR
HHCCCCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LIEAGGRIAAMGYDEASDLEETLDRAEAELFAVSQRRNNQDFVHIGRVVNTLFSQIESMQ
HHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ERRGEVIGVPTGYHDLDELTGGLQPSDLIILAARPSVGKTSLALSLAYNVAFHANGTVAI
HHCCCEEECCCCCCCHHHHCCCCCCCCEEEEEECCCCCCHHHEEEEEEEEEEECCCEEEE
FSLEMSREQLVQRMLAMHTGIDMQRLRTGNLRGEELSLAIEGLGVLSELPIYIEDTPGLS
EEECCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHEEHHHHHHHHHHCCEEEECCCCCC
ITDVRARARRLHSEVGITLVMIDYLQLMSGRRTDNRVQEVSDISRGLKALARELNVPVIA
CHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
LSQLSRAVEGRQNHVPMLSDLRESGSIEQDADIVMFIYREELYDKETDKKGIAEIHIAKH
HHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCEEEEEEHHHHHCCCCCCCCCEEEEEEEC
RNGPLGVIPLRFESRTTRFQNLERYQAPEGF
CCCCEEEEEEEECCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 1312531 [H]