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The map label for this gene is epsM [H]

Identifier: 222524310

GI number: 222524310

Start: 1314856

End: 1315509

Strand: Reverse

Name: epsM [H]

Synonym: Chy400_1033

Alternate gene names: 222524310

Gene position: 1315509-1314856 (Counterclockwise)

Preceding gene: 222524311

Following gene: 222524309

Centisome position: 24.97

GC content: 58.26

Gene sequence:

>654_bases
ATGACTCAGCGCATTGTCATCATCGGCGCAGGTGGTCACGCGCAGGTAGTGGCCGATATTCTGTTACGTTCTGCTGCCCA
CGGTCAGGCATGCCAGCCTATCGGTTACCTCGATGATAATCCAACATGTCACGGTCAGCAGCGCCTGGGGTTACCGGTGC
TGGGGCCGGTCAGCATGCTTGCCACACTTCCTCACGATAGTGTGATTGTTGCCATTGGCGATAATCGGGTGCGCGCCCGG
CTTTTCGCCGAATTGAAGGCGCGTGGCGAACATTTTGCCCGTGCCATTCATCCAACGGCGATTATTGCTCCTGACGTGGT
CATTGGGCCGGGCACCATGATCTGCGCCGGTGCCATTGTCAATCCCGGTAGCGTTATCGGTGCCAACGTTATCCTCAATA
CCGCCTGTACCGTCGATCACCACAATCAGGTGGGGGATCATGCGCATCTGGCACCGGGTGTACATACCGGTGGTGCGGTC
ACCATCGGCACCGGTGCTTTAGTTGGCATCGGCGCCATCGTGATGCCACAGCGGCGGGTTGGTGATTGGAGTGTCGTCGG
CGCCGGTGCGCTTGTCCACCGTGATGTTACTGCTGAGACAGTGGTTACCGGTGTACCGGCACAACCATTATATGTGCGGG
CTGTCGGCGAATAA

Upstream 100 bases:

>100_bases
GTATTGGATAACTGTCATTGCATACAGATAACAGACGGAGCAAGGCTTTCCTGCAATTCACCATACCCTGCGTTGCCCTG
TCGAGAGAGCATTGCAAACT

Downstream 100 bases:

>100_bases
CGGTCGATCTGCACAGCTCCAGTTGGAGCAACCGTCAGTGTTGTGTGTGTTCAAGTTTTACGCGATATACCAGGGGAGTT
GAGCGTGTCAGTTCCAATGT

Product: transferase hexapeptide repeat containing protein

Products: UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine; [Acyl-Carrier Protein]. [C]

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MTQRIVIIGAGGHAQVVADILLRSAAHGQACQPIGYLDDNPTCHGQQRLGLPVLGPVSMLATLPHDSVIVAIGDNRVRAR
LFAELKARGEHFARAIHPTAIIAPDVVIGPGTMICAGAIVNPGSVIGANVILNTACTVDHHNQVGDHAHLAPGVHTGGAV
TIGTGALVGIGAIVMPQRRVGDWSVVGAGALVHRDVTAETVVTGVPAQPLYVRAVGE

Sequences:

>Translated_217_residues
MTQRIVIIGAGGHAQVVADILLRSAAHGQACQPIGYLDDNPTCHGQQRLGLPVLGPVSMLATLPHDSVIVAIGDNRVRAR
LFAELKARGEHFARAIHPTAIIAPDVVIGPGTMICAGAIVNPGSVIGANVILNTACTVDHHNQVGDHAHLAPGVHTGGAV
TIGTGALVGIGAIVMPQRRVGDWSVVGAGALVHRDVTAETVVTGVPAQPLYVRAVGE
>Mature_216_residues
TQRIVIIGAGGHAQVVADILLRSAAHGQACQPIGYLDDNPTCHGQQRLGLPVLGPVSMLATLPHDSVIVAIGDNRVRARL
FAELKARGEHFARAIHPTAIIAPDVVIGPGTMICAGAIVNPGSVIGANVILNTACTVDHHNQVGDHAHLAPGVHTGGAVT
IGTGALVGIGAIVMPQRRVGDWSVVGAGALVHRDVTAETVVTGVPAQPLYVRAVGE

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR013817
- InterPro:   IPR020019
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.- [C]

Molecular weight: Translated: 22196; Mature: 22065

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQRIVIIGAGGHAQVVADILLRSAAHGQACQPIGYLDDNPTCHGQQRLGLPVLGPVSML
CCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
ATLPHDSVIVAIGDNRVRARLFAELKARGEHFARAIHPTAIIAPDVVIGPGTMICAGAIV
HHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCEEECCCCEEEECEEE
NPGSVIGANVILNTACTVDHHNQVGDHAHLAPGVHTGGAVTIGTGALVGIGAIVMPQRRV
CCCCEEECEEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHCEEEECCCCC
GDWSVVGAGALVHRDVTAETVVTGVPAQPLYVRAVGE
CCEEEECCCEEEECCCCHHHEEECCCCCCEEEEEECC
>Mature Secondary Structure 
TQRIVIIGAGGHAQVVADILLRSAAHGQACQPIGYLDDNPTCHGQQRLGLPVLGPVSML
CCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
ATLPHDSVIVAIGDNRVRARLFAELKARGEHFARAIHPTAIIAPDVVIGPGTMICAGAIV
HHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCEEECCCCEEEECEEE
NPGSVIGANVILNTACTVDHHNQVGDHAHLAPGVHTGGAVTIGTGALVGIGAIVMPQRRV
CCCCEEECEEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHCEEEECCCCC
GDWSVVGAGALVHRDVTAETVVTGVPAQPLYVRAVGE
CCEEEECCCEEEECCCCHHHEEECCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine; (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] [C]

Specific reaction: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine + (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] = UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine + [Acyl-Carrier Protein]. [C]

General reaction: Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]