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The map label for this gene is sigW [H]

Identifier: 222524287

GI number: 222524287

Start: 1287929

End: 1288531

Strand: Direct

Name: sigW [H]

Synonym: Chy400_1010

Alternate gene names: 222524287

Gene position: 1287929-1288531 (Clockwise)

Preceding gene: 222524286

Following gene: 222524288

Centisome position: 24.44

GC content: 51.74

Gene sequence:

>603_bases
ATGGCTAATGAAGAGCAACGACTGATCAGTGCAGCACAAGCGGGCGATCTCGATTCGTTTAACCAGATTGTACGGCTATA
CGAAACGCGGGTCTACAACCTTTGTTATCGTATGTTGGGCGATACCGATGCCGCAGCCGATGCCACCCAGGACACGTTTA
TCTCTGCTTTCCGTCATTTGGCGAACTTTCGCGGCGGTGCATTGAAATCCTGGCTCCTCCGCATTGCTACCAACACCTGC
TACGATGTACTACGTACCCGGAAACGGCGACCATCGGTGTCGCTTGACAAAGAGCCAGATGATGAGGGTAATCGGCTTGA
TCTCCCTGATCCGGGCGCCAGTCTCGATGAGCTTATGTTGCAGCAAGAGTTGAGTGCAGCGATCCAGCGTGGTTTGGCCG
AACTACCTGAAGATCAGCGCATCGTGATCATCCTCAGCGATATTCAAGGGTTAGCCTATGAGGAGATTGCCCACATTACC
GGTGTGCAGTTAGGAACTGTGAAATCGCGATTAAGTCGGGCACGGGCTAAATTGCGCGATATTCTCCGCGCCGGGGAACT
CTTACCGTTAAAATTTCGTCTTGATGATGAAACAACCACGTAA

Upstream 100 bases:

>100_bases
GAGTTGACGATATGCTCTTTCAACGGGCAAATATCGTTATCCCTCCTGACGCAAAACCGGTATAATACAGCTCAGGTTGG
TACAAGAGACGAGCGATCGT

Downstream 100 bases:

>100_bases
CAGTTACACCTGATGGTTACACGAGTACGCTGATGACAACCCTGTCGCCTTCACCATCTGAATTACCTGAGCATGATCGT
GAATTGCTCTCGGCATATAT

Product: ECF subfamily RNA polymerase sigma-24 subunit

Products: NA

Alternate protein names: Sigma-W factor [H]

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MANEEQRLISAAQAGDLDSFNQIVRLYETRVYNLCYRMLGDTDAAADATQDTFISAFRHLANFRGGALKSWLLRIATNTC
YDVLRTRKRRPSVSLDKEPDDEGNRLDLPDPGASLDELMLQQELSAAIQRGLAELPEDQRIVIILSDIQGLAYEEIAHIT
GVQLGTVKSRLSRARAKLRDILRAGELLPLKFRLDDETTT

Sequences:

>Translated_200_residues
MANEEQRLISAAQAGDLDSFNQIVRLYETRVYNLCYRMLGDTDAAADATQDTFISAFRHLANFRGGALKSWLLRIATNTC
YDVLRTRKRRPSVSLDKEPDDEGNRLDLPDPGASLDELMLQQELSAAIQRGLAELPEDQRIVIILSDIQGLAYEEIAHIT
GVQLGTVKSRLSRARAKLRDILRAGELLPLKFRLDDETTT
>Mature_199_residues
ANEEQRLISAAQAGDLDSFNQIVRLYETRVYNLCYRMLGDTDAAADATQDTFISAFRHLANFRGGALKSWLLRIATNTCY
DVLRTRKRRPSVSLDKEPDDEGNRLDLPDPGASLDELMLQQELSAAIQRGLAELPEDQRIVIILSDIQGLAYEEIAHITG
VQLGTVKSRLSRARAKLRDILRAGELLPLKFRLDDETTT

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma-W controls genes involved in transport processes and detoxification [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=177, Percent_Identity=38.9830508474576, Blast_Score=110, Evalue=8e-26,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014294
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 22447; Mature: 22316

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00622 HTH_LUXR_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANEEQRLISAAQAGDLDSFNQIVRLYETRVYNLCYRMLGDTDAAADATQDTFISAFRHL
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ANFRGGALKSWLLRIATNTCYDVLRTRKRRPSVSLDKEPDDEGNRLDLPDPGASLDELML
HHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCHHHHHH
QQELSAAIQRGLAELPEDQRIVIILSDIQGLAYEEIAHITGVQLGTVKSRLSRARAKLRD
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
ILRAGELLPLKFRLDDETTT
HHHCCCEEEEEEECCCCCCC
>Mature Secondary Structure 
ANEEQRLISAAQAGDLDSFNQIVRLYETRVYNLCYRMLGDTDAAADATQDTFISAFRHL
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ANFRGGALKSWLLRIATNTCYDVLRTRKRRPSVSLDKEPDDEGNRLDLPDPGASLDELML
HHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCHHHHHH
QQELSAAIQRGLAELPEDQRIVIILSDIQGLAYEEIAHITGVQLGTVKSRLSRARAKLRD
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
ILRAGELLPLKFRLDDETTT
HHHCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274029; 9384377; 12207695 [H]