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The map label for this gene is pfkA [H]

Identifier: 222524223

GI number: 222524223

Start: 1189555

End: 1191798

Strand: Direct

Name: pfkA [H]

Synonym: Chy400_0944

Alternate gene names: 222524223

Gene position: 1189555-1191798 (Clockwise)

Preceding gene: 222524222

Following gene: 222524235

Centisome position: 22.58

GC content: 58.51

Gene sequence:

>2244_bases
ATGACACATCCAACGATCCGAATTGGAGTCTTAACCAGTGGTGGCGATGCGCCGGGTATGAACGCCGCAGTACGGGCTGT
TGTACGCACCGGCATCAGTCGTGGCTGTGAAGTCTTTGCAATCTACGAAGGCTATCAGGGTCTGATCGAAGGTGGAAAAT
TGATCCGGCGCATGGATTGGAGTTCCGTTGGCGGCATCATCCAGCGTGGCGGTACGATCATTGGTACGGCGCGCAGCGCG
GCATTCCGCACCCGCGAGGGCCGACGCCGCGCAGCAGCGAATCTCTTGGCCCACGGGATCGACCGCCTGGTCGTGATCGG
TGGTGATGGCAGCCTCACCGGTGCCGACCTCTTTCGTCGTGAATGGCCGGAACTGCTGGCCGAACTGGTCGCAGCTGGTG
AGATCAGCAGCGAGCAGGCCGCCGCCCATCCCAATCTCCTCATTGCCGGGATTGTGGGCAGTATCGACAATGACTTCTGC
GGTACCGATATGACCATTGGTGCCGACACGGCGCTGCATCGTATCACCGAGGCGATTGATGCAATCAGCTCGACGGCGGC
CAGTCATCAGCGTACCTTTGTCATCGAGGTGATGGGGCGGAATTGTGGCTACCTGGCGCTGATGGGGGCGATTGCCGGTG
GCGCAGACTGGGCATTTATCCCTGAAATGCCTCCCCAAATGGATGACTGGGAGCAGCATATGTGCGATGTGCTGCGGATC
GGACGCGAACAGGGGCGACGCGACAGCATTGTGGTGGTAGCCGAAGGGGCGTGTGATCGCGACGGGAAACCAATCACGGC
TTCGTATGTCAAGAAAATTTTGGAAGAGCGTCTGGGTGAAGATACCAGAGTGACCATCCTCGGCCACGTGCAGCGTGGTG
GCGCTCCCAGCGCATTTGATCGCTGGATGAGTTCATTATTGGGGGCAACGGCGGTTGAAGAGTTACTGGAAGCCGATCCC
AATGCCGAACCGAAACTGATCGGGTTGCGTGAGAATCGGGTTGTGCGTCTACCACTGATGACCTGTGTCCGCGATACCCA
TCAGGTTGCCGAGGCGATTGCCCAGCGCGACTACGAACGTGCGCTGGCGATGCGGGGCAATAGCTTTGTGGAGGCGTACC
GCACCCTCGGCACCCTCATTCAGTCGCAGCCCTCGCCCCCCGACCGATTACGGCGCGGGCATCGCTTCGCAGTGCTGCAC
AGTGGCGGCCCGGCACCGGGAATGAATACGGCGGTACGGGCAGCGGTGCGGATCGGCATTGACCGCGGCCATACGATGCT
CGGTGTGCGCAATGGGTTTCAGGGACTGATCGATGGCGACATCCACGAAATGGATTGGATGAGCGTAAGTGGTTGGGCAA
CGATGGGTGGCGCCGAGCTGGGCACCAATCGCAAGGTGCCGCAAGGCAGCGAGCTATACCAGATTGCCCGTAACATCGAG
CGCTTCGGAATTGACGGTATCTTGATGATCGGTGGTTGGACCGGGTATCAGGCAATCTACAAGCTGTACAGCGAGCGACA
CATCTTCCCGGCATTCAACATCCCTATGATCTGCTTGCCGGCAACGATTGACAACAATCTTCCCGGTTCTGAGCTGAGTC
TGGGTGCAGATACCGCATTGAACAGCATCGTCAGTGCAATTGATCGGATCAAGCAGTCGGCGGTGGCTTCACGGCGCTGT
TTCATTGTCGAAGTTATGGGGCGTGACTGTGGTTATCTGGCCTTGATGAGCGGATTGGCAACCGGCGCCGAGCGGGTCTA
CCTCCCCGAAGAAGGCATCAGCCTGCGCAAATTGCAAGCCGATCTCGACGAGATGATGCGCTGGTTTCGTGCTGGTAAGC
GCCTGAGTTTGATCATTCGCAATGAAAAAGCGAATCCGATCTACACCACCTCGTTCATCTGCTCGTTGTTTGAAGAAGAG
GGTGGTAAGCTATTCGAGGTACGGCAGGCGATTCTCGGTCATTTGCAGCAGGGAGGTGATCCTTCGCCGTTCGACCGTAT
TCAGGCTACCCGTCTGGCCGTGCGCTGTATCGAGTTCCTGGTCGAGCACGCTGACCGTCGCGAACCAATCGGGGCGTTCA
TCGGCTATCGGGGCGGTAAAGTCCAGATACATCAGCTCGACGATATGCCACGCCTGATGGATCCGGTGCATGCCCGTCCG
CGAGAGCAGTGGTGGATGTCTTTGCGGCAAATGACCGATTTGCTCACGACGCCGCCATCACCTACCGTTGAGAGCGGGAA
GTAA

Upstream 100 bases:

>100_bases
TCGTCCGGCATGGAGACTCTGCGCATTCTTCACCCCGCGCTCCTGTGATATACTTGCGCATGCAACCATATCGCTATCCG
CCAAGAGGGCGAGGAATACA

Downstream 100 bases:

>100_bases
TAGCAGGTGCGCTGGCCCGGCAATGATGAATATCAAATCTGGTGCCACAGAAGGCTTGGCACACGATCCGAAAATGAGCC
TGATGCCGAACCTGAGCGTG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 747; Mature: 746

Protein sequence:

>747_residues
MTHPTIRIGVLTSGGDAPGMNAAVRAVVRTGISRGCEVFAIYEGYQGLIEGGKLIRRMDWSSVGGIIQRGGTIIGTARSA
AFRTREGRRRAAANLLAHGIDRLVVIGGDGSLTGADLFRREWPELLAELVAAGEISSEQAAAHPNLLIAGIVGSIDNDFC
GTDMTIGADTALHRITEAIDAISSTAASHQRTFVIEVMGRNCGYLALMGAIAGGADWAFIPEMPPQMDDWEQHMCDVLRI
GREQGRRDSIVVVAEGACDRDGKPITASYVKKILEERLGEDTRVTILGHVQRGGAPSAFDRWMSSLLGATAVEELLEADP
NAEPKLIGLRENRVVRLPLMTCVRDTHQVAEAIAQRDYERALAMRGNSFVEAYRTLGTLIQSQPSPPDRLRRGHRFAVLH
SGGPAPGMNTAVRAAVRIGIDRGHTMLGVRNGFQGLIDGDIHEMDWMSVSGWATMGGAELGTNRKVPQGSELYQIARNIE
RFGIDGILMIGGWTGYQAIYKLYSERHIFPAFNIPMICLPATIDNNLPGSELSLGADTALNSIVSAIDRIKQSAVASRRC
FIVEVMGRDCGYLALMSGLATGAERVYLPEEGISLRKLQADLDEMMRWFRAGKRLSLIIRNEKANPIYTTSFICSLFEEE
GGKLFEVRQAILGHLQQGGDPSPFDRIQATRLAVRCIEFLVEHADRREPIGAFIGYRGGKVQIHQLDDMPRLMDPVHARP
REQWWMSLRQMTDLLTTPPSPTVESGK

Sequences:

>Translated_747_residues
MTHPTIRIGVLTSGGDAPGMNAAVRAVVRTGISRGCEVFAIYEGYQGLIEGGKLIRRMDWSSVGGIIQRGGTIIGTARSA
AFRTREGRRRAAANLLAHGIDRLVVIGGDGSLTGADLFRREWPELLAELVAAGEISSEQAAAHPNLLIAGIVGSIDNDFC
GTDMTIGADTALHRITEAIDAISSTAASHQRTFVIEVMGRNCGYLALMGAIAGGADWAFIPEMPPQMDDWEQHMCDVLRI
GREQGRRDSIVVVAEGACDRDGKPITASYVKKILEERLGEDTRVTILGHVQRGGAPSAFDRWMSSLLGATAVEELLEADP
NAEPKLIGLRENRVVRLPLMTCVRDTHQVAEAIAQRDYERALAMRGNSFVEAYRTLGTLIQSQPSPPDRLRRGHRFAVLH
SGGPAPGMNTAVRAAVRIGIDRGHTMLGVRNGFQGLIDGDIHEMDWMSVSGWATMGGAELGTNRKVPQGSELYQIARNIE
RFGIDGILMIGGWTGYQAIYKLYSERHIFPAFNIPMICLPATIDNNLPGSELSLGADTALNSIVSAIDRIKQSAVASRRC
FIVEVMGRDCGYLALMSGLATGAERVYLPEEGISLRKLQADLDEMMRWFRAGKRLSLIIRNEKANPIYTTSFICSLFEEE
GGKLFEVRQAILGHLQQGGDPSPFDRIQATRLAVRCIEFLVEHADRREPIGAFIGYRGGKVQIHQLDDMPRLMDPVHARP
REQWWMSLRQMTDLLTTPPSPTVESGK
>Mature_746_residues
THPTIRIGVLTSGGDAPGMNAAVRAVVRTGISRGCEVFAIYEGYQGLIEGGKLIRRMDWSSVGGIIQRGGTIIGTARSAA
FRTREGRRRAAANLLAHGIDRLVVIGGDGSLTGADLFRREWPELLAELVAAGEISSEQAAAHPNLLIAGIVGSIDNDFCG
TDMTIGADTALHRITEAIDAISSTAASHQRTFVIEVMGRNCGYLALMGAIAGGADWAFIPEMPPQMDDWEQHMCDVLRIG
REQGRRDSIVVVAEGACDRDGKPITASYVKKILEERLGEDTRVTILGHVQRGGAPSAFDRWMSSLLGATAVEELLEADPN
AEPKLIGLRENRVVRLPLMTCVRDTHQVAEAIAQRDYERALAMRGNSFVEAYRTLGTLIQSQPSPPDRLRRGHRFAVLHS
GGPAPGMNTAVRAAVRIGIDRGHTMLGVRNGFQGLIDGDIHEMDWMSVSGWATMGGAELGTNRKVPQGSELYQIARNIER
FGIDGILMIGGWTGYQAIYKLYSERHIFPAFNIPMICLPATIDNNLPGSELSLGADTALNSIVSAIDRIKQSAVASRRCF
IVEVMGRDCGYLALMSGLATGAERVYLPEEGISLRKLQADLDEMMRWFRAGKRLSLIIRNEKANPIYTTSFICSLFEEEG
GKLFEVRQAILGHLQQGGDPSPFDRIQATRLAVRCIEFLVEHADRREPIGAFIGYRGGKVQIHQLDDMPRLMDPVHARPR
EQWWMSLRQMTDLLTTPPSPTVESGK

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI48762920, Length=739, Percent_Identity=50.8795669824087, Blast_Score=733, Evalue=0.0,
Organism=Homo sapiens, GI266453768, Length=739, Percent_Identity=49.5263870094723, Blast_Score=714, Evalue=0.0,
Organism=Homo sapiens, GI266453748, Length=739, Percent_Identity=49.5263870094723, Blast_Score=714, Evalue=0.0,
Organism=Homo sapiens, GI4505749, Length=739, Percent_Identity=49.5263870094723, Blast_Score=714, Evalue=0.0,
Organism=Homo sapiens, GI266453619, Length=739, Percent_Identity=49.5263870094723, Blast_Score=714, Evalue=0.0,
Organism=Homo sapiens, GI11321601, Length=744, Percent_Identity=49.1935483870968, Blast_Score=694, Evalue=0.0,
Organism=Escherichia coli, GI1790350, Length=311, Percent_Identity=43.0868167202572, Blast_Score=224, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI25147584, Length=752, Percent_Identity=47.4734042553192, Blast_Score=704, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17558788, Length=750, Percent_Identity=38.2666666666667, Blast_Score=503, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI72003601, Length=575, Percent_Identity=44.5217391304348, Blast_Score=501, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6321679, Length=693, Percent_Identity=48.6291486291486, Blast_Score=646, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323861, Length=763, Percent_Identity=41.6775884665793, Blast_Score=611, Evalue=1e-175,
Organism=Drosophila melanogaster, GI17647809, Length=736, Percent_Identity=49.5923913043478, Blast_Score=693, Evalue=0.0,
Organism=Drosophila melanogaster, GI24652337, Length=736, Percent_Identity=49.5923913043478, Blast_Score=693, Evalue=0.0,
Organism=Drosophila melanogaster, GI28573326, Length=736, Percent_Identity=49.1847826086957, Blast_Score=685, Evalue=0.0,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 81700; Mature: 81569

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHPTIRIGVLTSGGDAPGMNAAVRAVVRTGISRGCEVFAIYEGYQGLIEGGKLIRRMDW
CCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCH
SSVGGIIQRGGTIIGTARSAAFRTREGRRRAAANLLAHGIDRLVVIGGDGSLTGADLFRR
HHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHH
EWPELLAELVAAGEISSEQAAAHPNLLIAGIVGSIDNDFCGTDMTIGADTALHRITEAID
HHHHHHHHHHHHCCCCCHHHHCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AISSTAASHQRTFVIEVMGRNCGYLALMGAIAGGADWAFIPEMPPQMDDWEQHMCDVLRI
HHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHH
GREQGRRDSIVVVAEGACDRDGKPITASYVKKILEERLGEDTRVTILGHVQRGGAPSAFD
HHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHH
RWMSSLLGATAVEELLEADPNAEPKLIGLRENRVVRLPLMTCVRDTHQVAEAIAQRDYER
HHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
ALAMRGNSFVEAYRTLGTLIQSQPSPPDRLRRGHRFAVLHSGGPAPGMNTAVRAAVRIGI
HHHHCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCC
DRGHTMLGVRNGFQGLIDGDIHEMDWMSVSGWATMGGAELGTNRKVPQGSELYQIARNIE
CCCCEEEEECCCCCCCCCCCCHHCCCEEECCCEECCCCCCCCCCCCCCCHHHHHHHHHHH
RFGIDGILMIGGWTGYQAIYKLYSERHIFPAFNIPMICLPATIDNNLPGSELSLGADTAL
HHCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHH
NSIVSAIDRIKQSAVASRRCFIVEVMGRDCGYLALMSGLATGAERVYLPEEGISLRKLQA
HHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHH
DLDEMMRWFRAGKRLSLIIRNEKANPIYTTSFICSLFEEEGGKLFEVRQAILGHLQQGGD
HHHHHHHHHHCCCEEEEEEECCCCCCEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
PSPFDRIQATRLAVRCIEFLVEHADRREPIGAFIGYRGGKVQIHQLDDMPRLMDPVHARP
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEEEECCCCHHHHCHHCCCC
REQWWMSLRQMTDLLTTPPSPTVESGK
HHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
THPTIRIGVLTSGGDAPGMNAAVRAVVRTGISRGCEVFAIYEGYQGLIEGGKLIRRMDW
CCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCH
SSVGGIIQRGGTIIGTARSAAFRTREGRRRAAANLLAHGIDRLVVIGGDGSLTGADLFRR
HHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHH
EWPELLAELVAAGEISSEQAAAHPNLLIAGIVGSIDNDFCGTDMTIGADTALHRITEAID
HHHHHHHHHHHHCCCCCHHHHCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AISSTAASHQRTFVIEVMGRNCGYLALMGAIAGGADWAFIPEMPPQMDDWEQHMCDVLRI
HHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHH
GREQGRRDSIVVVAEGACDRDGKPITASYVKKILEERLGEDTRVTILGHVQRGGAPSAFD
HHHCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHH
RWMSSLLGATAVEELLEADPNAEPKLIGLRENRVVRLPLMTCVRDTHQVAEAIAQRDYER
HHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
ALAMRGNSFVEAYRTLGTLIQSQPSPPDRLRRGHRFAVLHSGGPAPGMNTAVRAAVRIGI
HHHHCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCC
DRGHTMLGVRNGFQGLIDGDIHEMDWMSVSGWATMGGAELGTNRKVPQGSELYQIARNIE
CCCCEEEEECCCCCCCCCCCCHHCCCEEECCCEECCCCCCCCCCCCCCCHHHHHHHHHHH
RFGIDGILMIGGWTGYQAIYKLYSERHIFPAFNIPMICLPATIDNNLPGSELSLGADTAL
HHCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHH
NSIVSAIDRIKQSAVASRRCFIVEVMGRDCGYLALMSGLATGAERVYLPEEGISLRKLQA
HHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHH
DLDEMMRWFRAGKRLSLIIRNEKANPIYTTSFICSLFEEEGGKLFEVRQAILGHLQQGGD
HHHHHHHHHHCCCEEEEEEECCCCCCEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
PSPFDRIQATRLAVRCIEFLVEHADRREPIGAFIGYRGGKVQIHQLDDMPRLMDPVHARP
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEEEECCCCHHHHCHHCCCC
REQWWMSLRQMTDLLTTPPSPTVESGK
HHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA