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The map label for this gene is dapE [H]

Identifier: 222524220

GI number: 222524220

Start: 1185301

End: 1186647

Strand: Direct

Name: dapE [H]

Synonym: Chy400_0941

Alternate gene names: 222524220

Gene position: 1185301-1186647 (Clockwise)

Preceding gene: 222524219

Following gene: 222524222

Centisome position: 22.5

GC content: 60.36

Gene sequence:

>1347_bases
ATGGAACGTTTTGCAGCTTACGTGGAAAGCCAGCGCTCACGCTTTCTGGCTGAATTGGCCGACATTGTACGTATCCCCTC
GGTGGCTGCCCAAAACCGTGGCATCGACGAGACGGCAAGCTTTGTCGAACAGCGCTTGCGTCGGTTAGGCGCCGAAACCC
GCCTCTTGCAGGCTGACGGCGGCAACCCGATGGTCTACGCCACGATTGGTAACGGCTCACGCACCCTGCTCATCTACGAC
CACTACGATGTGCAGCCGGCTGAGCCGTTTGAGTTGTGGCAGAGTCCGCCCTTTGAATTGACCGAACGTGACGGTAAGCT
CTACGGACGTGGTGTTGCCGACAACAAGGGCAACCTGATGCTGCGCATTCAGGCGATTGAGGCGTGGCTGGCGACTGAGG
GTGAACTGCCGATCCGCATTATCTTCGCCGTTGAAGGCGAAGAAGAGGTCAGTTCGCGCACCCTACCGGCGGTCTGTCGC
GAATATGCCGACCTCCTCCGCGCCGATGGCTGTCTCTGGGAGACAGGCGGGGTTGACATCAACGAACGACCGGTCGTAAC
CTGTGGCGCCAAAGGTATTCAATACGTCGAACTGGTGGCCCGTGGCGCCAGCTACGATCTCCACTCCTCGTATGCGACCT
TTGTGCCAAACCCGGCCTGGCGACTGACCTGGGCACTCAACACACTCAAAGGCCCTGATGGTCGGGTGCGCATCCCTGGC
TTCTACGACCGTGTCCGGCCCCCCTCAGCCGCCGATCTGGCCGCTCTGACCACCATTCCAACCAATGACGAAGAATTATT
GGCCGATTTTGGTCTGGATGAATTCCTTGATGGTCTGCGCGGTCTGGATCGTCTGCGCCGCCACCTGTTTGAACCAACCT
GTACGATTTGTGGGTTGGTGAGTGGTTACACCGGTGAAGGCAGCAAAACCGTCCTGCCCAACGAAGCGCGCGCCAAGATC
GACTTTCGCCTGGTACCGGATATGGAGCCGGCTGAAGTGTTGCACCAGTTGCGCCGGCATCTCGACGCCGAGGGCTTTGC
GGACATCGAAATTATTGATTTGGGTGGGGAACATCCGGCTCGTGGGCCGCTCGATGCAGCGATTGTCCAGGCAGCGCTCG
CCTCGGCCCGCGAGGTCTACGGCAGCGAACCCATCCTCTACCCCACCATGGCCGGTACCGGGCCGGTCTACCCACTGTGT
ACAGCATTGGGCACACCCGTCGCCAGTGGTGCCGGCTGCGGCTACCACGGCACGCGCGTCCACTCCCCGAACGAAAACAT
CCGGCTCGCCGACTACTGGCGTGCTATCCATTGGATGGGGAGTCTGATCCGACATTTCGCCGGGTAA

Upstream 100 bases:

>100_bases
GCGCATCGAGCAGGTATATGCCGAGGCGCTGACAGTATCACCCTGAAATGACAGTGCTATAATGACTGCCTGAACCGCTT
TTGTACAATTGGAGCAGCTC

Downstream 100 bases:

>100_bases
TGATCTCTGTCGCTCCAGGGGCGCACGTCTGTGCGTCCCTCCCTGCCAATGGTGAGAGCGTTTACCCGCTGTCGCATATT
AAATCCGGCTTACCAGGAGT

Product: peptidase M20

Products: NA

Alternate protein names: SDAP desuccinylase [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MERFAAYVESQRSRFLAELADIVRIPSVAAQNRGIDETASFVEQRLRRLGAETRLLQADGGNPMVYATIGNGSRTLLIYD
HYDVQPAEPFELWQSPPFELTERDGKLYGRGVADNKGNLMLRIQAIEAWLATEGELPIRIIFAVEGEEEVSSRTLPAVCR
EYADLLRADGCLWETGGVDINERPVVTCGAKGIQYVELVARGASYDLHSSYATFVPNPAWRLTWALNTLKGPDGRVRIPG
FYDRVRPPSAADLAALTTIPTNDEELLADFGLDEFLDGLRGLDRLRRHLFEPTCTICGLVSGYTGEGSKTVLPNEARAKI
DFRLVPDMEPAEVLHQLRRHLDAEGFADIEIIDLGGEHPARGPLDAAIVQAALASAREVYGSEPILYPTMAGTGPVYPLC
TALGTPVASGAGCGYHGTRVHSPNENIRLADYWRAIHWMGSLIRHFAG

Sequences:

>Translated_448_residues
MERFAAYVESQRSRFLAELADIVRIPSVAAQNRGIDETASFVEQRLRRLGAETRLLQADGGNPMVYATIGNGSRTLLIYD
HYDVQPAEPFELWQSPPFELTERDGKLYGRGVADNKGNLMLRIQAIEAWLATEGELPIRIIFAVEGEEEVSSRTLPAVCR
EYADLLRADGCLWETGGVDINERPVVTCGAKGIQYVELVARGASYDLHSSYATFVPNPAWRLTWALNTLKGPDGRVRIPG
FYDRVRPPSAADLAALTTIPTNDEELLADFGLDEFLDGLRGLDRLRRHLFEPTCTICGLVSGYTGEGSKTVLPNEARAKI
DFRLVPDMEPAEVLHQLRRHLDAEGFADIEIIDLGGEHPARGPLDAAIVQAALASAREVYGSEPILYPTMAGTGPVYPLC
TALGTPVASGAGCGYHGTRVHSPNENIRLADYWRAIHWMGSLIRHFAG
>Mature_448_residues
MERFAAYVESQRSRFLAELADIVRIPSVAAQNRGIDETASFVEQRLRRLGAETRLLQADGGNPMVYATIGNGSRTLLIYD
HYDVQPAEPFELWQSPPFELTERDGKLYGRGVADNKGNLMLRIQAIEAWLATEGELPIRIIFAVEGEEEVSSRTLPAVCR
EYADLLRADGCLWETGGVDINERPVVTCGAKGIQYVELVARGASYDLHSSYATFVPNPAWRLTWALNTLKGPDGRVRIPG
FYDRVRPPSAADLAALTTIPTNDEELLADFGLDEFLDGLRGLDRLRRHLFEPTCTICGLVSGYTGEGSKTVLPNEARAKI
DFRLVPDMEPAEVLHQLRRHLDAEGFADIEIIDLGGEHPARGPLDAAIVQAALASAREVYGSEPILYPTMAGTGPVYPLC
TALGTPVASGAGCGYHGTRVHSPNENIRLADYWRAIHWMGSLIRHFAG

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; fifth step. [C]

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family [H]

Homologues:

Organism=Homo sapiens, GI21071039, Length=468, Percent_Identity=27.5641025641026, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI271398239, Length=362, Percent_Identity=30.3867403314917, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI271398277, Length=224, Percent_Identity=26.3392857142857, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI1788816, Length=212, Percent_Identity=32.0754716981132, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17563146, Length=342, Percent_Identity=32.4561403508772, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI193211059, Length=474, Percent_Identity=27.6371308016878, Blast_Score=135, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6321159, Length=477, Percent_Identity=26.4150943396226, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6319758, Length=462, Percent_Identity=26.6233766233766, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24585880, Length=474, Percent_Identity=27.4261603375527, Blast_Score=156, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR010182
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 49243; Mature: 49243

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERFAAYVESQRSRFLAELADIVRIPSVAAQNRGIDETASFVEQRLRRLGAETRLLQADG
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHCCCHHEEEEECC
GNPMVYATIGNGSRTLLIYDHYDVQPAEPFELWQSPPFELTERDGKLYGRGVADNKGNLM
CCEEEEEEECCCCEEEEEEECCCCCCCCHHHHHCCCCCCEECCCCCEEECCCCCCCCCEE
LRIQAIEAWLATEGELPIRIIFAVEGEEEVSSRTLPAVCREYADLLRADGCLWETGGVDI
EEEEEEHHHHHCCCCCCEEEEEEECCCHHHHHCCHHHHHHHHHHHHHCCCCEEECCCCCC
NERPVVTCGAKGIQYVELVARGASYDLHSSYATFVPNPAWRLTWALNTLKGPDGRVRIPG
CCCCEEEECCCCHHHHHHHHCCCCCCCCCCCHHCCCCCCEEEEEEEECCCCCCCCEECCC
FYDRVRPPSAADLAALTTIPTNDEELLADFGLDEFLDGLRGLDRLRRHLFEPTCTICGLV
HHHHCCCCCCCCCEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
SGYTGEGSKTVLPNEARAKIDFRLVPDMEPAEVLHQLRRHLDAEGFADIEIIDLGGEHPA
CCCCCCCCCEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCC
RGPLDAAIVQAALASAREVYGSEPILYPTMAGTGPVYPLCTALGTPVASGAGCGYHGTRV
CCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEE
HSPNENIRLADYWRAIHWMGSLIRHFAG
ECCCCCEEHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MERFAAYVESQRSRFLAELADIVRIPSVAAQNRGIDETASFVEQRLRRLGAETRLLQADG
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHCCCHHEEEEECC
GNPMVYATIGNGSRTLLIYDHYDVQPAEPFELWQSPPFELTERDGKLYGRGVADNKGNLM
CCEEEEEEECCCCEEEEEEECCCCCCCCHHHHHCCCCCCEECCCCCEEECCCCCCCCCEE
LRIQAIEAWLATEGELPIRIIFAVEGEEEVSSRTLPAVCREYADLLRADGCLWETGGVDI
EEEEEEHHHHHCCCCCCEEEEEEECCCHHHHHCCHHHHHHHHHHHHHCCCCEEECCCCCC
NERPVVTCGAKGIQYVELVARGASYDLHSSYATFVPNPAWRLTWALNTLKGPDGRVRIPG
CCCCEEEECCCCHHHHHHHHCCCCCCCCCCCHHCCCCCCEEEEEEEECCCCCCCCEECCC
FYDRVRPPSAADLAALTTIPTNDEELLADFGLDEFLDGLRGLDRLRRHLFEPTCTICGLV
HHHHCCCCCCCCCEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
SGYTGEGSKTVLPNEARAKIDFRLVPDMEPAEVLHQLRRHLDAEGFADIEIIDLGGEHPA
CCCCCCCCCEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCC
RGPLDAAIVQAALASAREVYGSEPILYPTMAGTGPVYPLCTALGTPVASGAGCGYHGTRV
CCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEE
HSPNENIRLADYWRAIHWMGSLIRHFAG
ECCCCCEEHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA