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The map label for this gene is pimB [H]

Identifier: 222524052

GI number: 222524052

Start: 960147

End: 961391

Strand: Direct

Name: pimB [H]

Synonym: Chy400_0767

Alternate gene names: 222524052

Gene position: 960147-961391 (Clockwise)

Preceding gene: 222524051

Following gene: 222524053

Centisome position: 18.22

GC content: 62.49

Gene sequence:

>1245_bases
ATGCGCATCTTAATGCTGGCTTCATCACTACCAAAATATCCGCGAGAAACAACGGCACCGTTCATCGAAGAGATTGCCGC
CGGGATCGCGGCTCGTGGTCACGAGGTGACACTGGTGGCACCGTGGCATCCCGATCTGCGGCGCGGTACCTACGAACGTG
GGATTCATCTGCGTTTCTTCCGTTACGCACCGCATCCGGCACTCAATATCTGGGGCTATGCCCAATCATTGCAAAGCGAT
ACTCAGGTGCGCGAACGGGCGTGGCTGGCGGCGCCGTTTGCGCTTACTGCTTCGTTCAGTGCTCTGAGGCGCGAATTACA
CCAGGCGCGGGCCAGGGGAAAACCATTCGATCTGGTGCAGGCGCATTGGGTCTTACCCAACGGGCCGCCCGCTGCCCTCG
CAGCGCTCATGGCTCGCCTGCCGCTGGTGGTCAGCCTGCACGGCAGCGACATCTATCTGGCCGAGACACAACGCATCATG
GGTACCCTAGCCGGGCTGGTCTTTCAACGCGCTGCCGCCGTCACTGCCTGTAGTGCCGATCTGAGCATGCGAGGAGTACG
GTTGGGCGCTAACCCGACCCGTACCTTCGTCATTCCCTACGGTGTGCATCCCGATCAGTTTCGGCCTGATCCGGCAGCGG
CGCAGCAGTTTCGTACCGAATGGAATATTCCTGCCCACGCCCCGCTGGTGTTAGGGTTAGGGCGACTGGTGAGTAAAAAG
GGGTTCAGTGTTCTGCTCGATGCGTGGCCGGCGGTGTTGCGCATGCACCCTACAGCGCGTCTGGTTATCGTCGGCTACGG
CGATCTGCGTCCGGCCCTGGAAGCGCAGGCCGCCCGACTGGGTATTGCCACCACCGTGCTGTTTACCGGTCAGCTCGACC
GGGCGCGTACTGCGATGGCGATGGCCGCTGCCGATGTTTTTGCGCTGCCGATTGTGCGAGAGGGTGTTGACGGCTTGCCC
AACGTTTTGCTGGAAGCGATGGGGGCGGCGCGCCCGATTGTGGCGGCACGGGTAGCCGGGGTGCCCGATGTGATCGAGGA
TGGCGTGCATGGCCTGATTGTTCCCGAACGCGACGCAGCCGCCCTGGCAGCCGCTATTGGTCGCCTGATTGAAGATCGGG
CACTGGCAGCCCGCCTGGGGGCGGCAGCGCGCGAACGCATTCTGCATGAGCTGACGTGGGAGAATACAGCGGCACGCTAT
GAAGCTGCCTTTGCGGCTGCACTACGCGGTGAGGTCAGGCCATGA

Upstream 100 bases:

>100_bases
ATGATCATCGACGGTTTTGAATTGCTGTACGCCAGCCCAACACCTGATTTTCGGGCATTTATCTACCGTATCCCCGACTC
ATAATGGAACATCGTACCGT

Downstream 100 bases:

>100_bases
CGTGGCGATGGCTGATAGGCTGGCTTGTGATCGGCATCGCTGCGCTGGCCCTGCGCCTCTACCGGCTCGGCGATCAGAGT
CTCTGGCTGGATGAAGGCAA

Product: group 1 glycosyl transferase

Products: NA

Alternate protein names: Alpha-D-mannose-alpha-(1-6)-phosphatidylmyo-inositol-mannosyltransferase; Alpha-mannosyltransferase; Alpha-manT; Guanosine diphosphomannose-phosphatidyl-inositol alpha-mannosyltransferase; Phosphatidylinositol alpha-mannosyltransferase; PI alpha-mannosyltransferase [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MRILMLASSLPKYPRETTAPFIEEIAAGIAARGHEVTLVAPWHPDLRRGTYERGIHLRFFRYAPHPALNIWGYAQSLQSD
TQVRERAWLAAPFALTASFSALRRELHQARARGKPFDLVQAHWVLPNGPPAALAALMARLPLVVSLHGSDIYLAETQRIM
GTLAGLVFQRAAAVTACSADLSMRGVRLGANPTRTFVIPYGVHPDQFRPDPAAAQQFRTEWNIPAHAPLVLGLGRLVSKK
GFSVLLDAWPAVLRMHPTARLVIVGYGDLRPALEAQAARLGIATTVLFTGQLDRARTAMAMAAADVFALPIVREGVDGLP
NVLLEAMGAARPIVAARVAGVPDVIEDGVHGLIVPERDAAALAAAIGRLIEDRALAARLGAAARERILHELTWENTAARY
EAAFAAALRGEVRP

Sequences:

>Translated_414_residues
MRILMLASSLPKYPRETTAPFIEEIAAGIAARGHEVTLVAPWHPDLRRGTYERGIHLRFFRYAPHPALNIWGYAQSLQSD
TQVRERAWLAAPFALTASFSALRRELHQARARGKPFDLVQAHWVLPNGPPAALAALMARLPLVVSLHGSDIYLAETQRIM
GTLAGLVFQRAAAVTACSADLSMRGVRLGANPTRTFVIPYGVHPDQFRPDPAAAQQFRTEWNIPAHAPLVLGLGRLVSKK
GFSVLLDAWPAVLRMHPTARLVIVGYGDLRPALEAQAARLGIATTVLFTGQLDRARTAMAMAAADVFALPIVREGVDGLP
NVLLEAMGAARPIVAARVAGVPDVIEDGVHGLIVPERDAAALAAAIGRLIEDRALAARLGAAARERILHELTWENTAARY
EAAFAAALRGEVRP
>Mature_414_residues
MRILMLASSLPKYPRETTAPFIEEIAAGIAARGHEVTLVAPWHPDLRRGTYERGIHLRFFRYAPHPALNIWGYAQSLQSD
TQVRERAWLAAPFALTASFSALRRELHQARARGKPFDLVQAHWVLPNGPPAALAALMARLPLVVSLHGSDIYLAETQRIM
GTLAGLVFQRAAAVTACSADLSMRGVRLGANPTRTFVIPYGVHPDQFRPDPAAAQQFRTEWNIPAHAPLVLGLGRLVSKK
GFSVLLDAWPAVLRMHPTARLVIVGYGDLRPALEAQAARLGIATTVLFTGQLDRARTAMAMAAADVFALPIVREGVDGLP
NVLLEAMGAARPIVAARVAGVPDVIEDGVHGLIVPERDAAALAAAIGRLIEDRALAARLGAAARERILHELTWENTAARY
EAAFAAALRGEVRP

Specific function: Catalyzes the addition of a mannose residue from GDP-D- mannose to the position 6 of a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1) to generate phosphatidyl-myo-inositol bearing alpha-1,2- and alpha-1,6-linked mannose resid

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1788357, Length=291, Percent_Identity=26.4604810996564, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: =2.4.1.57 [H]

Molecular weight: Translated: 44758; Mature: 44758

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILMLASSLPKYPRETTAPFIEEIAAGIAARGHEVTLVAPWHPDLRRGTYERGIHLRFF
CEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHCCHHHCCCEEEEE
RYAPHPALNIWGYAQSLQSDTQVRERAWLAAPFALTASFSALRRELHQARARGKPFDLVQ
EECCCCCEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEE
AHWVLPNGPPAALAALMARLPLVVSLHGSDIYLAETQRIMGTLAGLVFQRAAAVTACSAD
EEEECCCCCHHHHHHHHHHCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCC
LSMRGVRLGANPTRTFVIPYGVHPDQFRPDPAAAQQFRTEWNIPAHAPLVLGLGRLVSKK
CCCCCEEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHC
GFSVLLDAWPAVLRMHPTARLVIVGYGDLRPALEAQAARLGIATTVLFTGQLDRARTAMA
CCHHHHHHHHHHHEECCCEEEEEEECCCCCHHHHHHHHHHHHHEEEEEECCCHHHHHHHH
MAAADVFALPIVREGVDGLPNVLLEAMGAARPIVAARVAGVPDVIEDGVHGLIVPERDAA
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHCCCCHHHHCCCCEEEECCCHHH
ALAAAIGRLIEDRALAARLGAAARERILHELTWENTAARYEAAFAAALRGEVRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRILMLASSLPKYPRETTAPFIEEIAAGIAARGHEVTLVAPWHPDLRRGTYERGIHLRFF
CEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHCCHHHCCCEEEEE
RYAPHPALNIWGYAQSLQSDTQVRERAWLAAPFALTASFSALRRELHQARARGKPFDLVQ
EECCCCCEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEE
AHWVLPNGPPAALAALMARLPLVVSLHGSDIYLAETQRIMGTLAGLVFQRAAAVTACSAD
EEEECCCCCHHHHHHHHHHCCEEEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCC
LSMRGVRLGANPTRTFVIPYGVHPDQFRPDPAAAQQFRTEWNIPAHAPLVLGLGRLVSKK
CCCCCEEECCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHC
GFSVLLDAWPAVLRMHPTARLVIVGYGDLRPALEAQAARLGIATTVLFTGQLDRARTAMA
CCHHHHHHHHHHHEECCCEEEEEEECCCCCHHHHHHHHHHHHHEEEEEECCCHHHHHHHH
MAAADVFALPIVREGVDGLPNVLLEAMGAARPIVAARVAGVPDVIEDGVHGLIVPERDAA
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHCCCCHHHHCCCCEEEECCCHHH
ALAAAIGRLIEDRALAARLGAAARERILHELTWENTAARYEAAFAAALRGEVRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]