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The map label for this gene is hpaIM [H]

Identifier: 222523998

GI number: 222523998

Start: 894913

End: 895926

Strand: Direct

Name: hpaIM [H]

Synonym: Chy400_0713

Alternate gene names: 222523998

Gene position: 894913-895926 (Clockwise)

Preceding gene: 222523997

Following gene: 222523999

Centisome position: 16.98

GC content: 47.63

Gene sequence:

>1014_bases
ATGCAAGATCACCACGATTCATATCCTTTGTTCCCCGAAGTCAGAGAGGCTTCGGCATCGTATACGCGGAACAAAGAACT
CCCCATGTTCTTTGGTCAGCCGATACGAAAGGGAACGATCCTCAATATCGAAGATGTGCCATCAGACCCGCACGAACCTG
TGCTCTACTACACGCACCCTCATGGAGAGATTTGGATTGGCGACGCCATTGCCTGGCTCCGGTCGCTTGAAACCGAGTCG
GTAGATATGATCTTTGCCGATCCACCATATAACATTCGCAAGGCAGAGTGGGATAGCTTCGAGTCGCAGGCAGCGTATGT
TGAATGGTCGTTAGAGTGGATCAGCGAAGCCGCTCGTGTTCTCAAACCTACCGGTACGTTATACATTTGTGGATTTTCCG
AAATTATTGCTGATCTCAAATTACCGGCATCGCGCTTTTTTAAAGGATGTCGCTGGCTGATCTGGCATTATAAAAACAAA
GCAAACCTTGGAAAAGACTGGGGACGTTCTCATGAAAGTATCCTTCATTTCCGCAAAGACAGACAATTTACATTCAACAT
TGATGATATTCGCATCCCTTATGGTCATCACACGCTGAAATATCCCGACCATCCACAGGCAGAGACCAGCCAATACAGCC
GGGGAAGACGACGCCAGGCTGTCTGGCGACCGCATCCACGCGGTGCAAAGCCGAGAGACGTGCTGGAAATACCAACCACC
TGCAACGGGATGCACGAGAAAACACCGCATCCAACCCAAAAACCTGAAGAATTGCTACGAAAGCTAGTTCTGGCATCCTC
GAATGTTGGTGATGTGATCGTTGATCCATTTCTTGGCTCTGGAACTACTGCGGTCGTTGCAGAACAACTCAAACGTCGGT
GGAAAGGATGTGATATTTCGCTCGAATATTGTCAATGGGCAGTCCAAAGAATTGAATTAGTAGAAGATTGGCCAATCGAA
AAATGGATTCAATACGATTTTGAGAATGCTGAACGAAGGAAATCCATTCGATGA

Upstream 100 bases:

>100_bases
CTGTTCTTGACAAACCCATACCTCTTTGCTACAGTGACGAACATCAATTGTGTTATCCACGCGAAACTCAGACAATACAC
CCAGAGATATAGCAAATCAT

Downstream 100 bases:

>100_bases
CCACAGTCACATTATCTGGTTTACAAGTCAGAGTACAGGATAAGACTGCCTTCCAGAGCCTTGATGAATACTACAAACTA
TGCTACGACTTTCTTTCATT

Product: DNA methylase N-4/N-6 domain-containing protein

Products: NA

Alternate protein names: M.HpaI; Adenine-specific methyltransferase HpaI [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MQDHHDSYPLFPEVREASASYTRNKELPMFFGQPIRKGTILNIEDVPSDPHEPVLYYTHPHGEIWIGDAIAWLRSLETES
VDMIFADPPYNIRKAEWDSFESQAAYVEWSLEWISEAARVLKPTGTLYICGFSEIIADLKLPASRFFKGCRWLIWHYKNK
ANLGKDWGRSHESILHFRKDRQFTFNIDDIRIPYGHHTLKYPDHPQAETSQYSRGRRRQAVWRPHPRGAKPRDVLEIPTT
CNGMHEKTPHPTQKPEELLRKLVLASSNVGDVIVDPFLGSGTTAVVAEQLKRRWKGCDISLEYCQWAVQRIELVEDWPIE
KWIQYDFENAERRKSIR

Sequences:

>Translated_337_residues
MQDHHDSYPLFPEVREASASYTRNKELPMFFGQPIRKGTILNIEDVPSDPHEPVLYYTHPHGEIWIGDAIAWLRSLETES
VDMIFADPPYNIRKAEWDSFESQAAYVEWSLEWISEAARVLKPTGTLYICGFSEIIADLKLPASRFFKGCRWLIWHYKNK
ANLGKDWGRSHESILHFRKDRQFTFNIDDIRIPYGHHTLKYPDHPQAETSQYSRGRRRQAVWRPHPRGAKPRDVLEIPTT
CNGMHEKTPHPTQKPEELLRKLVLASSNVGDVIVDPFLGSGTTAVVAEQLKRRWKGCDISLEYCQWAVQRIELVEDWPIE
KWIQYDFENAERRKSIR
>Mature_337_residues
MQDHHDSYPLFPEVREASASYTRNKELPMFFGQPIRKGTILNIEDVPSDPHEPVLYYTHPHGEIWIGDAIAWLRSLETES
VDMIFADPPYNIRKAEWDSFESQAAYVEWSLEWISEAARVLKPTGTLYICGFSEIIADLKLPASRFFKGCRWLIWHYKNK
ANLGKDWGRSHESILHFRKDRQFTFNIDDIRIPYGHHTLKYPDHPQAETSQYSRGRRRQAVWRPHPRGAKPRDVLEIPTT
CNGMHEKTPHPTQKPEELLRKLVLASSNVGDVIVDPFLGSGTTAVVAEQLKRRWKGCDISLEYCQWAVQRIELVEDWPIE
KWIQYDFENAERRKSIR

Specific function: This methylase recognizes the double-stranded sequence GTTAAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the HpaI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=260, Percent_Identity=35, Blast_Score=145, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 39439; Mature: 39439

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDHHDSYPLFPEVREASASYTRNKELPMFFGQPIRKGTILNIEDVPSDPHEPVLYYTHP
CCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHCCCCCCCCEEEECCCCCCCCCCEEEEECC
HGEIWIGDAIAWLRSLETESVDMIFADPPYNIRKAEWDSFESQAAYVEWSLEWISEAARV
CCCEEEHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHH
LKPTGTLYICGFSEIIADLKLPASRFFKGCRWLIWHYKNKANLGKDWGRSHESILHFRKD
HCCCCCEEEEEHHHHHHHHCCCHHHHHHHHEEEEEEECCCCCCCHHHCCCHHHHHHHHCC
RQFTFNIDDIRIPYGHHTLKYPDHPQAETSQYSRGRRRQAVWRPHPRGAKPRDVLEIPTT
CCEEEECCCEEECCCCCCCCCCCCCCHHHHHHHCCHHHCCCCCCCCCCCCCCCEEECCCC
CNGMHEKTPHPTQKPEELLRKLVLASSNVGDVIVDPFLGSGTTAVVAEQLKRRWKGCDIS
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEE
LEYCQWAVQRIELVEDWPIEKWIQYDFENAERRKSIR
HHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHCCC
>Mature Secondary Structure
MQDHHDSYPLFPEVREASASYTRNKELPMFFGQPIRKGTILNIEDVPSDPHEPVLYYTHP
CCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHCCCCCCCCEEEECCCCCCCCCCEEEEECC
HGEIWIGDAIAWLRSLETESVDMIFADPPYNIRKAEWDSFESQAAYVEWSLEWISEAARV
CCCEEEHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHH
LKPTGTLYICGFSEIIADLKLPASRFFKGCRWLIWHYKNKANLGKDWGRSHESILHFRKD
HCCCCCEEEEEHHHHHHHHCCCHHHHHHHHEEEEEEECCCCCCCHHHCCCHHHHHHHHCC
RQFTFNIDDIRIPYGHHTLKYPDHPQAETSQYSRGRRRQAVWRPHPRGAKPRDVLEIPTT
CCEEEECCCEEECCCCCCCCCCCCCCHHHHHHHCCHHHCCCCCCCCCCCCCCCEEECCCC
CNGMHEKTPHPTQKPEELLRKLVLASSNVGDVIVDPFLGSGTTAVVAEQLKRRWKGCDIS
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEE
LEYCQWAVQRIELVEDWPIEKWIQYDFENAERRKSIR
HHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1542567 [H]