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The map label for this gene is hsaB [H]

Identifier: 222523862

GI number: 222523862

Start: 681591

End: 682058

Strand: Direct

Name: hsaB [H]

Synonym: Chy400_0570

Alternate gene names: 222523862

Gene position: 681591-682058 (Clockwise)

Preceding gene: 222523861

Following gene: 222523863

Centisome position: 12.94

GC content: 56.2

Gene sequence:

>468_bases
ATGATCGACGAATCACTTTTTCGCCAAACGATGAGTCACTTCGCCTCCGGTGTCACTGTGGTGACGACTGCGCTCGGTGA
ACGCCTGGCCGGCTTAACAGTCAGCTCTTTCGCATCGCTATCACTGAATCCACCGCTGGTACTGATTTGTATTGATCACG
CGGCATCAAGCCATGACGTGATTGCCGCTGCCGGAAAGTTTGCGGTGAACATCCTGGCCGAAGATCAGGAATACCTCTCG
CGCCGGTTTGCCATGCACGACGGCGCAAAGTTTGCCCCGCATAGTTATACCCTGTCAGAGAACGGACTCCCACTTCTCGA
AGGGGTGCTGGCCCAGATCGAATGTCGGCTCCATAGCGCCTTGCCTGGCGGTGACCACACCATCTTCGTGGGAGAAGTCA
TCGCGGCCCGGGTGTTTGGGGGAAGACCATTGCTGTACTACCGATCAGGCTACCATCAGTTGGGGTAA

Upstream 100 bases:

>100_bases
TGTTGTTGCTCGACGATGATGCCCGCGCAGAGCATGCCGTTGGTGCCGAGATTCACGCTGCTATCGCTGCACGTCGCAAT
AGCCGAACAACAGGACGATC

Downstream 100 bases:

>100_bases
ATCGGTGAGATTCATCAAGCAGCCAGCAGGGTACCGCCTGGTGTTGTTGTGCTTCTTCCTGTACGGATTGATATTTCTAT
TGACAGCCTGTACAGTAACG

Product: flavin reductase domain-containing protein

Products: NAD(+); reduced flavin [C]

Alternate protein names: Flavin:NADH reductase [H]

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MIDESLFRQTMSHFASGVTVVTTALGERLAGLTVSSFASLSLNPPLVLICIDHAASSHDVIAAAGKFAVNILAEDQEYLS
RRFAMHDGAKFAPHSYTLSENGLPLLEGVLAQIECRLHSALPGGDHTIFVGEVIAARVFGGRPLLYYRSGYHQLG

Sequences:

>Translated_155_residues
MIDESLFRQTMSHFASGVTVVTTALGERLAGLTVSSFASLSLNPPLVLICIDHAASSHDVIAAAGKFAVNILAEDQEYLS
RRFAMHDGAKFAPHSYTLSENGLPLLEGVLAQIECRLHSALPGGDHTIFVGEVIAARVFGGRPLLYYRSGYHQLG
>Mature_155_residues
MIDESLFRQTMSHFASGVTVVTTALGERLAGLTVSSFASLSLNPPLVLICIDHAASSHDVIAAAGKFAVNILAEDQEYLS
RRFAMHDGAKFAPHSYTLSENGLPLLEGVLAQIECRLHSALPGGDHTIFVGEVIAARVFGGRPLLYYRSGYHQLG

Specific function: Catalyzes the reduction of free flavins (FMN or FAD) by NADH. Subsequently, the reduced flavins diffuse to the HsaA oxygenase subunit [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=157, Percent_Identity=28.6624203821656, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 16639; Mature: 16639

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDESLFRQTMSHFASGVTVVTTALGERLAGLTVSSFASLSLNPPLVLICIDHAASSHDV
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHEECCCCCEEEEEECCCCCCCCE
IAAAGKFAVNILAEDQEYLSRRFAMHDGAKFAPHSYTLSENGLPLLEGVLAQIECRLHSA
EEECCCEEEEEEECCHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHC
LPGGDHTIFVGEVIAARVFGGRPLLYYRSGYHQLG
CCCCCCEEEHHHHHHHHHCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MIDESLFRQTMSHFASGVTVVTTALGERLAGLTVSSFASLSLNPPLVLICIDHAASSHDV
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHEECCCCCEEEEEECCCCCCCCE
IAAAGKFAVNILAEDQEYLSRRFAMHDGAKFAPHSYTLSENGLPLLEGVLAQIECRLHSA
EEECCCEEEEEEECCHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHC
LPGGDHTIFVGEVIAARVFGGRPLLYYRSGYHQLG
CCCCCCEEEHHHHHHHHHCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]