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The map label for this gene is miaA

Identifier: 222523672

GI number: 222523672

Start: 445484

End: 446374

Strand: Direct

Name: miaA

Synonym: Chy400_0378

Alternate gene names: 222523672

Gene position: 445484-446374 (Clockwise)

Preceding gene: 222523668

Following gene: 222523675

Centisome position: 8.45

GC content: 59.37

Gene sequence:

>891_bases
TTGGAAGAGCTTATTGCCATTGTCGGACCGACCGCTGTCGGTAAGACAGAGCTGGCTGTCGCCTGGGCCAGACGTATCAA
CGGCGAGATTGTGTCTGCCGACTCACGGCAAATCTATCGCTGGATGGACATCGGTACTGCCAAGCCATCGCCTGAAGAGC
AGGCCCAGGCGCCGCACCACCTGATCGACATTCGTGATCCCGATCAACCGTTTTCACTGGCCGAGTTTTGCGATCTGGCC
ACGGCAGCAATCGCCGACATTCGTGGCCGGGGGCGCATCCCACTTCTGGTTGGCGGTACCGGTCAATACCTGGCAGCCTT
CCTGGAAGGCTGGCAGGTACCACGTGTGGCGCCACAACCTGACCTGCGTGCCGAACTTGAACTCATTGCCGCTCGTGAAG
GTCCGGCAGTGCTCCACGCACGTCTGGCCGCCGTCGATCCGGTGGCAGCATCACGCATTCCCCCTACGAATGTTCGGCGT
GTGGTACGAGCGCTGGAAGTGTACCTTGTCAGCGGTGAACCGATTTCGCGTCTGCAAGAGCGAAAAGAGCCTCCTTTTCG
CCCACGCACCATCTGGCTCCATCGGCCACGCGCCGAATTGTATGCCCGTGCCGATGCGCGGATTGAACGTATGATCGCTG
CCGGTCTGGTCGAGGAAGTTGCCGGTCTGCTGGCCCGTGGCTACGACTGGTCGCTTCCGGCAATGTCGAGTCTCGGTTAT
ATTCAGTTTCGACCATATTTTACCGGTGAAGCCGATCTACCGACCTGTATCGAGCGGCTACGCTTCGACACCCATGCCTT
CATCCGCCGCCAGGAGATGTGGTTTCGGCGTCTGCCCAATCTTGAAATCTGGACTCCTGATCATCCGTCCTGGCGTGAGA
TCACGGCTTGA

Upstream 100 bases:

>100_bases
AGAATGAAACGGACGTGAACATAAATAACGCAGTGCGTTATACCTGTATGATATGATGGTGAGGTGAAATACACATTATT
ACGTCAGATGAGGAACACGC

Downstream 100 bases:

>100_bases
GCGACGTGGTACTCGCGCAGCACATGTTCAATAAAACGTTCTGGATCGCCAGAACCCGATGGCAAAGCCAGCCGCTGCCG
GAGAACAGCCGCCAGATGAG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MEELIAIVGPTAVGKTELAVAWARRINGEIVSADSRQIYRWMDIGTAKPSPEEQAQAPHHLIDIRDPDQPFSLAEFCDLA
TAAIADIRGRGRIPLLVGGTGQYLAAFLEGWQVPRVAPQPDLRAELELIAAREGPAVLHARLAAVDPVAASRIPPTNVRR
VVRALEVYLVSGEPISRLQERKEPPFRPRTIWLHRPRAELYARADARIERMIAAGLVEEVAGLLARGYDWSLPAMSSLGY
IQFRPYFTGEADLPTCIERLRFDTHAFIRRQEMWFRRLPNLEIWTPDHPSWREITA

Sequences:

>Translated_296_residues
MEELIAIVGPTAVGKTELAVAWARRINGEIVSADSRQIYRWMDIGTAKPSPEEQAQAPHHLIDIRDPDQPFSLAEFCDLA
TAAIADIRGRGRIPLLVGGTGQYLAAFLEGWQVPRVAPQPDLRAELELIAAREGPAVLHARLAAVDPVAASRIPPTNVRR
VVRALEVYLVSGEPISRLQERKEPPFRPRTIWLHRPRAELYARADARIERMIAAGLVEEVAGLLARGYDWSLPAMSSLGY
IQFRPYFTGEADLPTCIERLRFDTHAFIRRQEMWFRRLPNLEIWTPDHPSWREITA
>Mature_296_residues
MEELIAIVGPTAVGKTELAVAWARRINGEIVSADSRQIYRWMDIGTAKPSPEEQAQAPHHLIDIRDPDQPFSLAEFCDLA
TAAIADIRGRGRIPLLVGGTGQYLAAFLEGWQVPRVAPQPDLRAELELIAAREGPAVLHARLAAVDPVAASRIPPTNVRR
VVRALEVYLVSGEPISRLQERKEPPFRPRTIWLHRPRAELYARADARIERMIAAGLVEEVAGLLARGYDWSLPAMSSLGY
IQFRPYFTGEADLPTCIERLRFDTHAFIRRQEMWFRRLPNLEIWTPDHPSWREITA

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=313, Percent_Identity=33.5463258785943, Blast_Score=157, Evalue=1e-38,
Organism=Escherichia coli, GI1790613, Length=276, Percent_Identity=42.0289855072464, Blast_Score=188, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI25144712, Length=274, Percent_Identity=25.5474452554745, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324848, Length=291, Percent_Identity=29.553264604811, Blast_Score=149, Evalue=6e-37,
Organism=Drosophila melanogaster, GI24649830, Length=360, Percent_Identity=30, Blast_Score=149, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_CHLAA (A9WDJ6)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001633991.1
- ProteinModelPortal:   A9WDJ6
- SMR:   A9WDJ6
- GeneID:   5825751
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_0351
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 33373; Mature: 33373

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEELIAIVGPTAVGKTELAVAWARRINGEIVSADSRQIYRWMDIGTAKPSPEEQAQAPHH
CCCEEEEECCCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHEECCCCCCCCHHHHCCCCE
LIDIRDPDQPFSLAEFCDLATAAIADIRGRGRIPLLVGGTGQYLAAFLEGWQVPRVAPQP
EEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCC
DLRAELELIAAREGPAVLHARLAAVDPVAASRIPPTNVRRVVRALEVYLVSGEPISRLQE
CHHHHHHHEECCCCCHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHEECCCHHHHHHH
RKEPPFRPRTIWLHRPRAELYARADARIERMIAAGLVEEVAGLLARGYDWSLPAMSSLGY
HCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCE
IQFRPYFTGEADLPTCIERLRFDTHAFIRRQEMWFRRLPNLEIWTPDHPSWREITA
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCC
>Mature Secondary Structure
MEELIAIVGPTAVGKTELAVAWARRINGEIVSADSRQIYRWMDIGTAKPSPEEQAQAPHH
CCCEEEEECCCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHEECCCCCCCCHHHHCCCCE
LIDIRDPDQPFSLAEFCDLATAAIADIRGRGRIPLLVGGTGQYLAAFLEGWQVPRVAPQP
EEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCC
DLRAELELIAAREGPAVLHARLAAVDPVAASRIPPTNVRRVVRALEVYLVSGEPISRLQE
CHHHHHHHEECCCCCHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHEECCCHHHHHHH
RKEPPFRPRTIWLHRPRAELYARADARIERMIAAGLVEEVAGLLARGYDWSLPAMSSLGY
HCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCE
IQFRPYFTGEADLPTCIERLRFDTHAFIRRQEMWFRRLPNLEIWTPDHPSWREITA
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA