Click here to switch to the map view.

The map label for this gene is aksF [H]

Identifier: 222523650

GI number: 222523650

Start: 422342

End: 423340

Strand: Direct

Name: aksF [H]

Synonym: Chy400_0356

Alternate gene names: 222523650

Gene position: 422342-423340 (Clockwise)

Preceding gene: 222523649

Following gene: 222523651

Centisome position: 8.02

GC content: 60.06

Gene sequence:

>999_bases
ATGCAGATCTGTGTTATTCCCGGCGATGGGATCGGCCCGGAAGTCATAGCCGTGGCAACTGCGGCCCTGCGTGTGCTGGC
ACCCGATCTGACCATCAAAGAGGCAGAAGCGGGATGGGCTGTCTTTCAGCGCACCGGTACGGCACTTCCTGAAACAACCC
TGGCTCTGGCGCGTGAGGCGACGGCGATCCTCTTTGGTGCGGTCGCGTCACCAAGCCATCCGGTTCCCGGTTATCGCAGT
CCGATTGTCGAGTTGCGGCGCACCCTCGACCTTTACGCCAATATTCGACCAACCGTGGGCAATGAGGTTGACCTGGTGGT
TGTGCGTGAAAACACCGAAGACCTGTACATCGGGCGGGAACGGTTAGAAGATGATGGCGCGACCGCCATTGCCGAACGTG
TCATTACCCGGGCCGCCTCGGCCCGGATCGTGCGTACCGCCTGCGAACTGGCTCGCACGCGCCAGGCTTACGGCCATCCC
GGCAAAGTGACTATCGTCCACAAAGCCAATGTCTTGCGGGTCAGCGACGGTCTTTTCCGCACGGTAGCGCTCGAAGTCGC
CGCCGATTATCCCGAACTGACCTTCGAGGAACGGCTGGTTGATGTTGCGGCCATGCAACTCGCTGCGCAACCGCAACGAT
TTGACGTGATCGTAACCACCAACATGTTCGGCGATATTTTGTCGGACATTGCCTGCATTCACGGCGGTGGATTGGGCGTT
GCCGCCAGCAGTAATCTCGGCCATGGCCGGGCGTTGTTTGAACCAGTGCATGGTGCAGCGCCCGACATTGCCGGACGGGG
AATCGCTAATCCAACCGCAGCACTCAATTGTGTCGTAATGCTGCTCGACTGGATCGGGCGTCCGCATGCGGCGGAACGCC
TCCGTTCTGCCATCACGGCTGTGGCGGCTGCCGGCATTCGTACACCCGATGTCGGTGGTCAGGCAACCACCCGCGAAGTC
GCCGATGAGATTCTTAACCAGTTATCTGCTCAGATGTGA

Upstream 100 bases:

>100_bases
ATCTGCCACCGCCACCGCTCTTCTTGCGAGAAGTCTGGGCGGCGGGAGGGATTGTGCCCTTTTACCGTACCTACGGACGT
TTTCCCGGTGAGGTGGCCTG

Downstream 100 bases:

>100_bases
AAGGAGAATCAGCCATGCACGCCGAATGCCCTGAATGTGTTGCCCAGATTACCTTACCTGCCAGCACGCTGGAGAGCGAA
ATTGTCGCCTGTCCCGACTG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: HICDH; Isohomocitrate dehydrogenase; IHDH; NAD-dependent threo-isohomocitrate dehydrogenase [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MQICVIPGDGIGPEVIAVATAALRVLAPDLTIKEAEAGWAVFQRTGTALPETTLALAREATAILFGAVASPSHPVPGYRS
PIVELRRTLDLYANIRPTVGNEVDLVVVRENTEDLYIGRERLEDDGATAIAERVITRAASARIVRTACELARTRQAYGHP
GKVTIVHKANVLRVSDGLFRTVALEVAADYPELTFEERLVDVAAMQLAAQPQRFDVIVTTNMFGDILSDIACIHGGGLGV
AASSNLGHGRALFEPVHGAAPDIAGRGIANPTAALNCVVMLLDWIGRPHAAERLRSAITAVAAAGIRTPDVGGQATTREV
ADEILNQLSAQM

Sequences:

>Translated_332_residues
MQICVIPGDGIGPEVIAVATAALRVLAPDLTIKEAEAGWAVFQRTGTALPETTLALAREATAILFGAVASPSHPVPGYRS
PIVELRRTLDLYANIRPTVGNEVDLVVVRENTEDLYIGRERLEDDGATAIAERVITRAASARIVRTACELARTRQAYGHP
GKVTIVHKANVLRVSDGLFRTVALEVAADYPELTFEERLVDVAAMQLAAQPQRFDVIVTTNMFGDILSDIACIHGGGLGV
AASSNLGHGRALFEPVHGAAPDIAGRGIANPTAALNCVVMLLDWIGRPHAAERLRSAITAVAAAGIRTPDVGGQATTREV
ADEILNQLSAQM
>Mature_332_residues
MQICVIPGDGIGPEVIAVATAALRVLAPDLTIKEAEAGWAVFQRTGTALPETTLALAREATAILFGAVASPSHPVPGYRS
PIVELRRTLDLYANIRPTVGNEVDLVVVRENTEDLYIGRERLEDDGATAIAERVITRAASARIVRTACELARTRQAYGHP
GKVTIVHKANVLRVSDGLFRTVALEVAADYPELTFEERLVDVAAMQLAAQPQRFDVIVTTNMFGDILSDIACIHGGGLGV
AASSNLGHGRALFEPVHGAAPDIAGRGIANPTAALNCVVMLLDWIGRPHAAERLRSAITAVAAAGIRTPDVGGQATTREV
ADEILNQLSAQM

Specific function: Catalyzes the NAD-dependent oxidation and decarboxylation of (2R,3S)-homoisocitrate ((1R,2S)-1- hydroxybutane-1,2,4-tricarboxylate), (2R,3S)-homo(2)-isocitrate and (2R,3S)-homo(3)-isocitrate, into 2-oxoadipate, 2-oxopimelate, and 2-oxosuberate, respective

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=345, Percent_Identity=37.3913043478261, Blast_Score=194, Evalue=9e-50,
Organism=Homo sapiens, GI4758582, Length=338, Percent_Identity=35.207100591716, Blast_Score=184, Evalue=9e-47,
Organism=Homo sapiens, GI28178838, Length=309, Percent_Identity=35.5987055016181, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI28178821, Length=343, Percent_Identity=31.4868804664723, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI28178816, Length=340, Percent_Identity=31.7647058823529, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI28178819, Length=203, Percent_Identity=34.4827586206897, Blast_Score=112, Evalue=6e-25,
Organism=Escherichia coli, GI1788101, Length=361, Percent_Identity=37.3961218836565, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI87081683, Length=359, Percent_Identity=35.3760445682451, Blast_Score=171, Evalue=9e-44,
Organism=Escherichia coli, GI1787381, Length=375, Percent_Identity=28.2666666666667, Blast_Score=114, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17505779, Length=342, Percent_Identity=36.5497076023392, Blast_Score=188, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI71986051, Length=331, Percent_Identity=38.0664652567976, Blast_Score=187, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI25144293, Length=352, Percent_Identity=34.9431818181818, Blast_Score=174, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI17550882, Length=343, Percent_Identity=31.4868804664723, Blast_Score=166, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6322097, Length=348, Percent_Identity=42.816091954023, Blast_Score=255, Evalue=9e-69,
Organism=Saccharomyces cerevisiae, GI6324709, Length=341, Percent_Identity=36.950146627566, Blast_Score=195, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6324291, Length=347, Percent_Identity=34.5821325648415, Blast_Score=169, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6319830, Length=359, Percent_Identity=30.6406685236769, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24643268, Length=343, Percent_Identity=36.1516034985423, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI24643270, Length=343, Percent_Identity=36.1516034985423, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI24661184, Length=343, Percent_Identity=35.2769679300292, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI161078637, Length=343, Percent_Identity=32.3615160349854, Blast_Score=165, Evalue=3e-41,
Organism=Drosophila melanogaster, GI161078633, Length=343, Percent_Identity=32.3615160349854, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24650122, Length=343, Percent_Identity=32.3615160349854, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI161078635, Length=341, Percent_Identity=31.6715542521994, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI161078639, Length=341, Percent_Identity=32.5513196480938, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI281362242, Length=340, Percent_Identity=33.8235294117647, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24648872, Length=340, Percent_Identity=33.8235294117647, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI20130355, Length=236, Percent_Identity=31.7796610169492, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.87 [H]

Molecular weight: Translated: 35141; Mature: 35141

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQICVIPGDGIGPEVIAVATAALRVLAPDLTIKEAEAGWAVFQRTGTALPETTLALAREA
CEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCCCHHHHHHHHHH
TAILFGAVASPSHPVPGYRSPIVELRRTLDLYANIRPTVGNEVDLVVVRENTEDLYIGRE
HHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEHHHH
RLEDDGATAIAERVITRAASARIVRTACELARTRQAYGHPGKVTIVHKANVLRVSDGLFR
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEECCCHHH
TVALEVAADYPELTFEERLVDVAAMQLAAQPQRFDVIVTTNMFGDILSDIACIHGGGLGV
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCE
AASSNLGHGRALFEPVHGAAPDIAGRGIANPTAALNCVVMLLDWIGRPHAAERLRSAITA
EECCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VAAAGIRTPDVGGQATTREVADEILNQLSAQM
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQICVIPGDGIGPEVIAVATAALRVLAPDLTIKEAEAGWAVFQRTGTALPETTLALAREA
CEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCCCHHHHHHHHHH
TAILFGAVASPSHPVPGYRSPIVELRRTLDLYANIRPTVGNEVDLVVVRENTEDLYIGRE
HHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEHHHH
RLEDDGATAIAERVITRAASARIVRTACELARTRQAYGHPGKVTIVHKANVLRVSDGLFR
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEECCCHHH
TVALEVAADYPELTFEERLVDVAAMQLAAQPQRFDVIVTTNMFGDILSDIACIHGGGLGV
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCE
AASSNLGHGRALFEPVHGAAPDIAGRGIANPTAALNCVVMLLDWIGRPHAAERLRSAITA
EECCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VAAAGIRTPDVGGQATTREVADEILNQLSAQM
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087; 10940051 [H]