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The map label for this gene is leuD [H]

Identifier: 222523649

GI number: 222523649

Start: 421842

End: 422342

Strand: Direct

Name: leuD [H]

Synonym: Chy400_0355

Alternate gene names: 222523649

Gene position: 421842-422342 (Clockwise)

Preceding gene: 222523648

Following gene: 222523650

Centisome position: 8.01

GC content: 57.88

Gene sequence:

>501_bases
ATGGCTCGCGTGTGGCTCTTCGGCCCCGATATCAATACCGACCAGATCGTACCCGGTCGCTACGCACCCTACATGCTGAA
AGACGAGAGTGAATTACGGCGCTATCCGTTTATCGAGCATCGGCCAGATTTTGCGGCCAATGTGCGACCGGGCGACATCA
TCGTAGCCGGCAAGAACTTTGGTTGCGGCAGTTCGCGTGAATACGCCCCGCTGGCGCTGCGAATGGTGGGTATCGGCGCG
ATTATTGCTCCCCTCTTTGCCCGCATCTTCTTTCGGAATGCGCTCAATCTTGGCATTCCATGCTTTACCGCCGATCTTAC
CGGCGAGCTAGCCGATGGTGATGAGGTTGAACTTGATCTGGAACAGGGCCGTATCACAACCGCCGATGGGCGCGTGATTC
ATCTGCCACCGCCACCGCTCTTCTTGCGAGAAGTCTGGGCGGCGGGAGGGATTGTGCCCTTTTACCGTACCTACGGACGT
TTTCCCGGTGAGGTGGCCTGA

Upstream 100 bases:

>100_bases
GCCACAGCACTTACCGGCTATCTAACTGATCCGCGCACGGTGATGGATGGACAACCGGCTATCGCTTCCCGTAACTAAGT
GAAGAAAGAGAGGTATCGCT

Downstream 100 bases:

>100_bases
TGCAGATCTGTGTTATTCCCGGCGATGGGATCGGCCCGGAAGTCATAGCCGTGGCAACTGCGGCCCTGCGTGTGCTGGCA
CCCGATCTGACCATCAAAGA

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MARVWLFGPDINTDQIVPGRYAPYMLKDESELRRYPFIEHRPDFAANVRPGDIIVAGKNFGCGSSREYAPLALRMVGIGA
IIAPLFARIFFRNALNLGIPCFTADLTGELADGDEVELDLEQGRITTADGRVIHLPPPPLFLREVWAAGGIVPFYRTYGR
FPGEVA

Sequences:

>Translated_166_residues
MARVWLFGPDINTDQIVPGRYAPYMLKDESELRRYPFIEHRPDFAANVRPGDIIVAGKNFGCGSSREYAPLALRMVGIGA
IIAPLFARIFFRNALNLGIPCFTADLTGELADGDEVELDLEQGRITTADGRVIHLPPPPLFLREVWAAGGIVPFYRTYGR
FPGEVA
>Mature_165_residues
ARVWLFGPDINTDQIVPGRYAPYMLKDESELRRYPFIEHRPDFAANVRPGDIIVAGKNFGCGSSREYAPLALRMVGIGAI
IAPLFARIFFRNALNLGIPCFTADLTGELADGDEVELDLEQGRITTADGRVIHLPPPPLFLREVWAAGGIVPFYRTYGRF
PGEVA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6320440, Length=132, Percent_Identity=35.6060606060606, Blast_Score=69, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6321429, Length=146, Percent_Identity=32.1917808219178, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 18393; Mature: 18262

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARVWLFGPDINTDQIVPGRYAPYMLKDESELRRYPFIEHRPDFAANVRPGDIIVAGKNF
CCEEEEECCCCCCCCCCCCCCCCEEECCHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCC
GCGSSREYAPLALRMVGIGAIIAPLFARIFFRNALNLGIPCFTADLTGELADGDEVELDL
CCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEEE
EQGRITTADGRVIHLPPPPLFLREVWAAGGIVPFYRTYGRFPGEVA
CCCEEEECCCEEEECCCCCHHHHHHHHCCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure 
ARVWLFGPDINTDQIVPGRYAPYMLKDESELRRYPFIEHRPDFAANVRPGDIIVAGKNF
CEEEEECCCCCCCCCCCCCCCCEEECCHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCC
GCGSSREYAPLALRMVGIGAIIAPLFARIFFRNALNLGIPCFTADLTGELADGDEVELDL
CCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEEE
EQGRITTADGRVIHLPPPPLFLREVWAAGGIVPFYRTYGRFPGEVA
CCCEEEECCCEEEECCCCCHHHHHHHHCCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]