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The map label for this gene is leuC [H]

Identifier: 222523648

GI number: 222523648

Start: 420563

End: 421819

Strand: Direct

Name: leuC [H]

Synonym: Chy400_0354

Alternate gene names: 222523648

Gene position: 420563-421819 (Clockwise)

Preceding gene: 222523647

Following gene: 222523649

Centisome position: 7.98

GC content: 60.86

Gene sequence:

>1257_bases
ATGCCAACGATGAGCGAGCAAATTTTGAGCCGTGTCGCCGGGCGGACGGTGCGCGCCGGTGATGTGGTGACGGCAAACGT
TGATCTGGTGATGGTGCACGATAGTCTGGCCCCCGGTATTATTCGCATCTTGCATCAAGAGTTAGGTGCAGAACGGGTCT
GGGATCCGCAACGGGTCGCCGTCGTGATCGATCACGTCGCCCCCGCCGCCAGTGTCCAGACCGCCGAGAAGCAGCAAGAG
GTGCGGCGCTGGGTACGTGCTCAGGGCATTCCTCATCTGTTTGATGTTGGGCGTGGCATCTCGCACCCGGTGCTGGTCGA
GGAAGGGCTGGCCCAGCCGGGGATGCTCATTCTGGGGAGTGATAGCCACAGCACGGCCTATGGCTGCGTGGGTGCGTTTG
GTACCGGAATGGGCAGTACCGATATTGCCCTGGCGCTGGCCACCGGCAAGACATGGCTGCGCGTACCTGAAACAATTGTT
GTCCGGGCACGCGGTAGGTTTGGATTTGGCGTTGGCCCCAAAGACCTGGCGCTGCGTGCGGCCCGTTTGCTGCGTGCCGA
TGGCGCAACTTACGCCGCAATTGAATGGCACGGTGTCGAATTCCTGAGTGTGATGGAGCGTATGACGCTGGCGACACTTT
CAATTGAAATGGGAGCAAAAGCCGGCATTGTGCCGCCGACCGGTCTCAATGTCACCGGCCCGCTCTTGCCCACAGTCACC
GCTGATGCCGGCTATCAGGAAGTGGTTGAGATCGATCTCGACCAGCTTGAACCACAAGTCTCAGCTCCCCATTACGTTGA
CAACGTCGCCAACCTCAGCGATCTGGGTCGGGTCGCAGTTGATGTCGTGTACCTCGGCACCTGCACCAACGGTCATTATG
AAGATATGGCAGTTGCCGCCCAGATTCTGGCCGGACGCCGACTCGCGCCGGGTGTGCGCATGATTGTGGTACCAGCAAGT
GCGCAGGCCCTGCAGCGTGCTGCTGCCGATGGCACACTCGCGACGCTCCTGGCTGCCGGTGCCACCATCGGCACACCGGG
ATGCGGTGCCTGCATCGGTCGTCACATGGGAGTCCTTGCCCCAGGCGAGGTCTGTCTGTTTACCGGCAACCGCAACTTCC
GTGGTCGGATGGGTAGCCCTGAAGCGCAAATCTATCTGGCATCGCCGGCGGTTGCCGCAGCCACAGCACTTACCGGCTAT
CTAACTGATCCGCGCACGGTGATGGATGGACAACCGGCTATCGCTTCCCGTAACTAA

Upstream 100 bases:

>100_bases
CGCTGCCCGGATCAAGGCACTCGGTGACGAACACGATCTTGATGGCGCAATGATTGACGAGATTCTCTATAGCTACGCAG
AGTAAGGGGAGGATACGGCT

Downstream 100 bases:

>100_bases
GTGAAGAAAGAGAGGTATCGCTATGGCTCGCGTGTGGCTCTTCGGCCCCGATATCAATACCGACCAGATCGTACCCGGTC
GCTACGCACCCTACATGCTG

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MPTMSEQILSRVAGRTVRAGDVVTANVDLVMVHDSLAPGIIRILHQELGAERVWDPQRVAVVIDHVAPAASVQTAEKQQE
VRRWVRAQGIPHLFDVGRGISHPVLVEEGLAQPGMLILGSDSHSTAYGCVGAFGTGMGSTDIALALATGKTWLRVPETIV
VRARGRFGFGVGPKDLALRAARLLRADGATYAAIEWHGVEFLSVMERMTLATLSIEMGAKAGIVPPTGLNVTGPLLPTVT
ADAGYQEVVEIDLDQLEPQVSAPHYVDNVANLSDLGRVAVDVVYLGTCTNGHYEDMAVAAQILAGRRLAPGVRMIVVPAS
AQALQRAAADGTLATLLAAGATIGTPGCGACIGRHMGVLAPGEVCLFTGNRNFRGRMGSPEAQIYLASPAVAAATALTGY
LTDPRTVMDGQPAIASRN

Sequences:

>Translated_418_residues
MPTMSEQILSRVAGRTVRAGDVVTANVDLVMVHDSLAPGIIRILHQELGAERVWDPQRVAVVIDHVAPAASVQTAEKQQE
VRRWVRAQGIPHLFDVGRGISHPVLVEEGLAQPGMLILGSDSHSTAYGCVGAFGTGMGSTDIALALATGKTWLRVPETIV
VRARGRFGFGVGPKDLALRAARLLRADGATYAAIEWHGVEFLSVMERMTLATLSIEMGAKAGIVPPTGLNVTGPLLPTVT
ADAGYQEVVEIDLDQLEPQVSAPHYVDNVANLSDLGRVAVDVVYLGTCTNGHYEDMAVAAQILAGRRLAPGVRMIVVPAS
AQALQRAAADGTLATLLAAGATIGTPGCGACIGRHMGVLAPGEVCLFTGNRNFRGRMGSPEAQIYLASPAVAAATALTGY
LTDPRTVMDGQPAIASRN
>Mature_417_residues
PTMSEQILSRVAGRTVRAGDVVTANVDLVMVHDSLAPGIIRILHQELGAERVWDPQRVAVVIDHVAPAASVQTAEKQQEV
RRWVRAQGIPHLFDVGRGISHPVLVEEGLAQPGMLILGSDSHSTAYGCVGAFGTGMGSTDIALALATGKTWLRVPETIVV
RARGRFGFGVGPKDLALRAARLLRADGATYAAIEWHGVEFLSVMERMTLATLSIEMGAKAGIVPPTGLNVTGPLLPTVTA
DAGYQEVVEIDLDQLEPQVSAPHYVDNVANLSDLGRVAVDVVYLGTCTNGHYEDMAVAAQILAGRRLAPGVRMIVVPASA
QALQRAAADGTLATLLAAGATIGTPGCGACIGRHMGVLAPGEVCLFTGNRNFRGRMGSPEAQIYLASPAVAAATALTGYL
TDPRTVMDGQPAIASRN

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=377, Percent_Identity=30.2387267904509, Blast_Score=123, Evalue=4e-28,
Organism=Escherichia coli, GI1786259, Length=446, Percent_Identity=31.390134529148, Blast_Score=165, Evalue=6e-42,
Organism=Escherichia coli, GI87081781, Length=350, Percent_Identity=26, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787531, Length=391, Percent_Identity=23.2736572890026, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI2367097, Length=334, Percent_Identity=26.3473053892216, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25149337, Length=357, Percent_Identity=29.9719887955182, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI32564738, Length=375, Percent_Identity=29.3333333333333, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI25149342, Length=297, Percent_Identity=29.6296296296296, Blast_Score=101, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6320440, Length=449, Percent_Identity=32.0712694877506, Blast_Score=200, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6321429, Length=447, Percent_Identity=28.4116331096197, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6323335, Length=355, Percent_Identity=31.2676056338028, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6322261, Length=436, Percent_Identity=26.3761467889908, Blast_Score=120, Evalue=3e-28,
Organism=Drosophila melanogaster, GI28571643, Length=353, Percent_Identity=31.728045325779, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI281365315, Length=358, Percent_Identity=29.608938547486, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17864292, Length=358, Percent_Identity=29.608938547486, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161076999, Length=358, Percent_Identity=29.608938547486, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 43766; Mature: 43635

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTMSEQILSRVAGRTVRAGDVVTANVDLVMVHDSLAPGIIRILHQELGAERVWDPQRVA
CCCHHHHHHHHHHCCEEECCCEEEECEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEE
VVIDHVAPAASVQTAEKQQEVRRWVRAQGIPHLFDVGRGISHPVLVEEGLAQPGMLILGS
EEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEHHCCCCCCCEEEEEC
DSHSTAYGCVGAFGTGMGSTDIALALATGKTWLRVPETIVVRARGRFGFGVGPKDLALRA
CCCCCHHHHHHHHCCCCCCCCEEEEEECCCEEEECCCEEEEEECCCCCCCCCHHHHHHHH
ARLLRADGATYAAIEWHGVEFLSVMERMTLATLSIEMGAKAGIVPPTGLNVTGPLLPTVT
HHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCEEE
ADAGYQEVVEIDLDQLEPQVSAPHYVDNVANLSDLGRVAVDVVYLGTCTNGHYEDMAVAA
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCHHHHHHHEEEEEEEEECCCCCHHHHHHHH
QILAGRRLAPGVRMIVVPASAQALQRAAADGTLATLLAAGATIGTPGCGACIGRHMGVLA
HHHHCCCCCCCCEEEEEECCHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHCCCCC
PGEVCLFTGNRNFRGRMGSPEAQIYLASPAVAAATALTGYLTDPRTVMDGQPAIASRN
CCCEEEEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCEEECCCCCCCCCC
>Mature Secondary Structure 
PTMSEQILSRVAGRTVRAGDVVTANVDLVMVHDSLAPGIIRILHQELGAERVWDPQRVA
CCHHHHHHHHHHCCEEECCCEEEECEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEE
VVIDHVAPAASVQTAEKQQEVRRWVRAQGIPHLFDVGRGISHPVLVEEGLAQPGMLILGS
EEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEHHCCCCCCCEEEEEC
DSHSTAYGCVGAFGTGMGSTDIALALATGKTWLRVPETIVVRARGRFGFGVGPKDLALRA
CCCCCHHHHHHHHCCCCCCCCEEEEEECCCEEEECCCEEEEEECCCCCCCCCHHHHHHHH
ARLLRADGATYAAIEWHGVEFLSVMERMTLATLSIEMGAKAGIVPPTGLNVTGPLLPTVT
HHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCCCEEE
ADAGYQEVVEIDLDQLEPQVSAPHYVDNVANLSDLGRVAVDVVYLGTCTNGHYEDMAVAA
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCHHHHHHHEEEEEEEEECCCCCHHHHHHHH
QILAGRRLAPGVRMIVVPASAQALQRAAADGTLATLLAAGATIGTPGCGACIGRHMGVLA
HHHHCCCCCCCCEEEEEECCHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHCCCCC
PGEVCLFTGNRNFRGRMGSPEAQIYLASPAVAAATALTGYLTDPRTVMDGQPAIASRN
CCCEEEEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA