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The map label for this gene is lys20 [H]

Identifier: 222523647

GI number: 222523647

Start: 419420

End: 420547

Strand: Direct

Name: lys20 [H]

Synonym: Chy400_0353

Alternate gene names: 222523647

Gene position: 419420-420547 (Clockwise)

Preceding gene: 222523646

Following gene: 222523648

Centisome position: 7.96

GC content: 56.38

Gene sequence:

>1128_bases
ATGTCACTGCCTGAGCGCTTGTTTTTTGTTGATACGACATTGCGCGAAGGCGAACAATTCGCCAGCGCTCGCTTTACAAC
TGCTCAACGAATTGCCATTGCTGAAATGCTCGACGCCTTTGGCGTAGAGTATATCGAACTCACCTCCCCTGCTGCCTCAC
CACAAAGTGCGCGTGATCTTGCAACTATCGCCCGTCGCGGTTTACGTGCCCGCATCCTCACCCATATCCGCTGCCATATG
GCCGATGCTCGGCTCGCTGTTGAACACGGCGCGCAGGGTGCAAATCTGCTCTTTGCCACGTCCGAACCGTTACGCACGGT
AAGCCACGGTCGCAGCCTGAGCGAAATCCTGGCTGAGGCCAAACGTGTGATCTGCTATCTCCGTGACCACGACATTGAAG
TACGCTTTTCGTGCGAAGATAGCTTTCGTACCGACCTCGCCGATCTGATCCGTATTTACCGCGCCGTTGAAACAATGGGT
GTGCAACGGGTCGGTCTGGCTGATACGGTGGGCATCGCCACCCCACGCCAGGTCTACGACGTGGTGCGGGCAGTACGTAA
CGAAGTACAGTGCGACATCGAATTTCACGGCCACAATGATAGCGGCTGCGCAATTGCCAATACCTTCTCGGCCTATGAGG
CCGGTGCAACGCATCTTGATGTGACGGTACTCGGCATCGGTGAACGGAATGGGATCGCCAGTTTGAGTGGTATGATTGCG
CGCATCGCCAGCATTGATCCATCCCGCGTGCAACGCTATCGCCTCGATCTGCTGCCCAAAATTGATGAGACGGTTGCAAC
GATGCTCGGCATTGAGATTCCGTTCAACCAGTGTATTACCAGTCCGACCGCGTTCCACCACAAGGCTGGGATGCATACCA
AAGCGGTGTTGAGCGACCCGCGCAGCTATGAAGTGCTTGATCCCAATCTGTTCGGTCGGCAGCGCACTATCGCGATTGCC
CATCGGCTGGTTGGATGGCACGCTGTCGCCGAACGTGCGCGTGAACTGGGCATTACGCTCAGCGAAGCCCAGGCCCGTGC
TGCCGCTGCCCGGATCAAGGCACTCGGTGACGAACACGATCTTGATGGCGCAATGATTGACGAGATTCTCTATAGCTACG
CAGAGTAA

Upstream 100 bases:

>100_bases
AGTATGAAACACATTGCTAGCGTCAGCCCAATCGCCTACACGCCACGCTATTGCCGGCCAGAACTCTTTGGCTCCTGTCA
AGTGAGAGAGGTGCTGATCT

Downstream 100 bases:

>100_bases
GGGGAGGATACGGCTATGCCAACGATGAGCGAGCAAATTTTGAGCCGTGTCGCCGGGCGGACGGTGCGCGCCGGTGATGT
GGTGACGGCAAACGTTGATC

Product: pyruvate carboxyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MSLPERLFFVDTTLREGEQFASARFTTAQRIAIAEMLDAFGVEYIELTSPAASPQSARDLATIARRGLRARILTHIRCHM
ADARLAVEHGAQGANLLFATSEPLRTVSHGRSLSEILAEAKRVICYLRDHDIEVRFSCEDSFRTDLADLIRIYRAVETMG
VQRVGLADTVGIATPRQVYDVVRAVRNEVQCDIEFHGHNDSGCAIANTFSAYEAGATHLDVTVLGIGERNGIASLSGMIA
RIASIDPSRVQRYRLDLLPKIDETVATMLGIEIPFNQCITSPTAFHHKAGMHTKAVLSDPRSYEVLDPNLFGRQRTIAIA
HRLVGWHAVAERARELGITLSEAQARAAAARIKALGDEHDLDGAMIDEILYSYAE

Sequences:

>Translated_375_residues
MSLPERLFFVDTTLREGEQFASARFTTAQRIAIAEMLDAFGVEYIELTSPAASPQSARDLATIARRGLRARILTHIRCHM
ADARLAVEHGAQGANLLFATSEPLRTVSHGRSLSEILAEAKRVICYLRDHDIEVRFSCEDSFRTDLADLIRIYRAVETMG
VQRVGLADTVGIATPRQVYDVVRAVRNEVQCDIEFHGHNDSGCAIANTFSAYEAGATHLDVTVLGIGERNGIASLSGMIA
RIASIDPSRVQRYRLDLLPKIDETVATMLGIEIPFNQCITSPTAFHHKAGMHTKAVLSDPRSYEVLDPNLFGRQRTIAIA
HRLVGWHAVAERARELGITLSEAQARAAAARIKALGDEHDLDGAMIDEILYSYAE
>Mature_374_residues
SLPERLFFVDTTLREGEQFASARFTTAQRIAIAEMLDAFGVEYIELTSPAASPQSARDLATIARRGLRARILTHIRCHMA
DARLAVEHGAQGANLLFATSEPLRTVSHGRSLSEILAEAKRVICYLRDHDIEVRFSCEDSFRTDLADLIRIYRAVETMGV
QRVGLADTVGIATPRQVYDVVRAVRNEVQCDIEFHGHNDSGCAIANTFSAYEAGATHLDVTVLGIGERNGIASLSGMIAR
IASIDPSRVQRYRLDLLPKIDETVATMLGIEIPFNQCITSPTAFHHKAGMHTKAVLSDPRSYEVLDPNLFGRQRTIAIAH
RLVGWHAVAERARELGITLSEAQARAAAARIKALGDEHDLDGAMIDEILYSYAE

Specific function: Catalyzes The Condensation Of The Acetyl Group Of Acetyl-CoA With 3-Methyl-2-Oxobutanoate (2-Oxoisovalerate) To Form 3-Carboxy-3-Hydroxy-4-Methylpentanoate (2-Isopropylmalate). [C]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=240, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1786261, Length=357, Percent_Identity=29.6918767507003, Blast_Score=149, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6320071, Length=374, Percent_Identity=50, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6320019, Length=366, Percent_Identity=50.5464480874317, Blast_Score=389, Evalue=1e-109,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011872
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.14 [H]

Molecular weight: Translated: 41307; Mature: 41175

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPERLFFVDTTLREGEQFASARFTTAQRIAIAEMLDAFGVEYIELTSPAASPQSARDL
CCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHH
ATIARRGLRARILTHIRCHMADARLAVEHGAQGANLLFATSEPLRTVSHGRSLSEILAEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHCCCCHHHHHHHH
KRVICYLRDHDIEVRFSCEDSFRTDLADLIRIYRAVETMGVQRVGLADTVGIATPRQVYD
HHHEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHH
VVRAVRNEVQCDIEFHGHNDSGCAIANTFSAYEAGATHLDVTVLGIGERNGIASLSGMIA
HHHHHHHCCEEEEEEECCCCCCCEEECCHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHH
RIASIDPSRVQRYRLDLLPKIDETVATMLGIEIPFNQCITSPTAFHHKAGMHTKAVLSDP
HHHCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHCCCCHHHHHCCCCHHHHHCCC
RSYEVLDPNLFGRQRTIAIAHRLVGWHAVAERARELGITLSEAQARAAAARIKALGDEHD
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
LDGAMIDEILYSYAE
CCHHHHHHHHHHHCC
>Mature Secondary Structure 
SLPERLFFVDTTLREGEQFASARFTTAQRIAIAEMLDAFGVEYIELTSPAASPQSARDL
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHH
ATIARRGLRARILTHIRCHMADARLAVEHGAQGANLLFATSEPLRTVSHGRSLSEILAEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHCCCCHHHHHHHH
KRVICYLRDHDIEVRFSCEDSFRTDLADLIRIYRAVETMGVQRVGLADTVGIATPRQVYD
HHHEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHH
VVRAVRNEVQCDIEFHGHNDSGCAIANTFSAYEAGATHLDVTVLGIGERNGIASLSGMIA
HHHHHHHCCEEEEEEECCCCCCCEEECCHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHH
RIASIDPSRVQRYRLDLLPKIDETVATMLGIEIPFNQCITSPTAFHHKAGMHTKAVLSDP
HHHCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHCCCCHHHHHCCCCHHHHHCCC
RSYEVLDPNLFGRQRTIAIAHRLVGWHAVAERARELGITLSEAQARAAAARIKALGDEHD
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
LDGAMIDEILYSYAE
CCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9868782 [H]