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The map label for this gene is speE [H]

Identifier: 222151906

GI number: 222151906

Start: 1800718

End: 1801578

Strand: Direct

Name: speE [H]

Synonym: MCCL_1663

Alternate gene names: 222151906

Gene position: 1800718-1801578 (Clockwise)

Preceding gene: 222151905

Following gene: 222151907

Centisome position: 85.65

GC content: 38.1

Gene sequence:

>861_bases
ATGTTAGATTTATGGTATACAGAGAATCACACGGACAACGTAAAATTTTCTATTAAAACAACAGAACATCTATTAACCGA
AAAAACACCATTCCAGCAAATTGATTTCTTCAAGTCTGAAGAATTTGGTACGTTCTTTACGTTAGATGGTTTAATGATGG
TAAACGAAAAAGATGAATTCGTATATCACGATATGATTACACACGTTGCAATGGCAACAAACCCTAATATTAAGAAAGTA
CTCGTTATCGGTGGCGGTGATGGGGGTACAGTACGAGAACTGACACGTTATGCCTCCCTCGAACAAATCGACATGGTAGA
AATTGATGAGCGCGTTGTGCGTTTATCTCAAGAATATCTTCCGATTACAGCAGGAAAACTCTCAGATCCACGTGTCACTT
TATTATTCGAAGACGGTTTAGCATATGTTAAGAATAAAGAAGATGGTTACTACGATTTAATTTTAGTAGATTCTACTGAT
CCTATCGGTCCTGGTGAAGGTTTATTCTCACATGACTTCTATAACAACTGCTTCCGTGTATTAACAGATGACGGTATTTT
AATCAATCAGCATGAATCACCGTATTATCCAAGCTACGCACATGAAATGAAGCGTGCACACTCTAAGATCAAAGGCTTAT
TCCCGATTACGCGTGTGTACCAGTTCCATATGCCAACTTACCCATCAGGTCACTGGTTATTCGGATTTGCATCTAAAAAG
TTCGACCCATTAAAAGATGCTGACTTAGATGCATGGAATGCACTTGGCTTAAAAACAAAATATTATAACACAGATATTCA
TACAGGTGCATTCGCCCTTCCAACATTCGTTAAGGAGATGCTCGATGATGCAACCGAATAA

Upstream 100 bases:

>100_bases
CGTGGTATGGCTGATAAAATTCACAAATATTCTAATGGTGAAATCGTCGTAGAATCATTCAAACCTGTGGAAGTAGCAGC
TAAATAAGGAGGAATTATAC

Downstream 100 bases:

>100_bases
ACATGTATATATGAGCTGCGAAGCTTCATTCGATGAAGCGACAACAGTAATATATGGCGCGCCATTTGACGGTACTGTTT
CAAACCGCCCTGGCACACGC

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MLDLWYTENHTDNVKFSIKTTEHLLTEKTPFQQIDFFKSEEFGTFFTLDGLMMVNEKDEFVYHDMITHVAMATNPNIKKV
LVIGGGDGGTVRELTRYASLEQIDMVEIDERVVRLSQEYLPITAGKLSDPRVTLLFEDGLAYVKNKEDGYYDLILVDSTD
PIGPGEGLFSHDFYNNCFRVLTDDGILINQHESPYYPSYAHEMKRAHSKIKGLFPITRVYQFHMPTYPSGHWLFGFASKK
FDPLKDADLDAWNALGLKTKYYNTDIHTGAFALPTFVKEMLDDATE

Sequences:

>Translated_286_residues
MLDLWYTENHTDNVKFSIKTTEHLLTEKTPFQQIDFFKSEEFGTFFTLDGLMMVNEKDEFVYHDMITHVAMATNPNIKKV
LVIGGGDGGTVRELTRYASLEQIDMVEIDERVVRLSQEYLPITAGKLSDPRVTLLFEDGLAYVKNKEDGYYDLILVDSTD
PIGPGEGLFSHDFYNNCFRVLTDDGILINQHESPYYPSYAHEMKRAHSKIKGLFPITRVYQFHMPTYPSGHWLFGFASKK
FDPLKDADLDAWNALGLKTKYYNTDIHTGAFALPTFVKEMLDDATE
>Mature_286_residues
MLDLWYTENHTDNVKFSIKTTEHLLTEKTPFQQIDFFKSEEFGTFFTLDGLMMVNEKDEFVYHDMITHVAMATNPNIKKV
LVIGGGDGGTVRELTRYASLEQIDMVEIDERVVRLSQEYLPITAGKLSDPRVTLLFEDGLAYVKNKEDGYYDLILVDSTD
PIGPGEGLFSHDFYNNCFRVLTDDGILINQHESPYYPSYAHEMKRAHSKIKGLFPITRVYQFHMPTYPSGHWLFGFASKK
FDPLKDADLDAWNALGLKTKYYNTDIHTGAFALPTFVKEMLDDATE

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=288, Percent_Identity=33.6805555555556, Blast_Score=189, Evalue=3e-48,
Organism=Escherichia coli, GI1786312, Length=263, Percent_Identity=40.6844106463878, Blast_Score=195, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI25154106, Length=274, Percent_Identity=35.4014598540146, Blast_Score=161, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI212645616, Length=325, Percent_Identity=31.3846153846154, Blast_Score=148, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6325326, Length=273, Percent_Identity=35.8974358974359, Blast_Score=178, Evalue=9e-46,
Organism=Saccharomyces cerevisiae, GI6323175, Length=292, Percent_Identity=33.5616438356164, Blast_Score=167, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24645443, Length=284, Percent_Identity=34.8591549295775, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI21355941, Length=221, Percent_Identity=35.2941176470588, Blast_Score=137, Evalue=7e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 32816; Mature: 32816

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLWYTENHTDNVKFSIKTTEHLLTEKTPFQQIDFFKSEEFGTFFTLDGLMMVNEKDEF
CEEEEEECCCCCCEEEEEECHHHHHHCCCCHHHHHCCCCCCCCEEEEECCEEEECCCCCH
VYHDMITHVAMATNPNIKKVLVIGGGDGGTVRELTRYASLEQIDMVEIDERVVRLSQEYL
HHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHC
PITAGKLSDPRVTLLFEDGLAYVKNKEDGYYDLILVDSTDPIGPGEGLFSHDFYNNCFRV
CEECCCCCCCEEEEEEECCEEEEECCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHE
LTDDGILINQHESPYYPSYAHEMKRAHSKIKGLFPITRVYQFHMPTYPSGHWLFGFASKK
EECCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCCEEEEECCCC
FDPLKDADLDAWNALGLKTKYYNTDIHTGAFALPTFVKEMLDDATE
CCCCCCCCCCCHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDLWYTENHTDNVKFSIKTTEHLLTEKTPFQQIDFFKSEEFGTFFTLDGLMMVNEKDEF
CEEEEEECCCCCCEEEEEECHHHHHHCCCCHHHHHCCCCCCCCEEEEECCEEEECCCCCH
VYHDMITHVAMATNPNIKKVLVIGGGDGGTVRELTRYASLEQIDMVEIDERVVRLSQEYL
HHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHC
PITAGKLSDPRVTLLFEDGLAYVKNKEDGYYDLILVDSTDPIGPGEGLFSHDFYNNCFRV
CEECCCCCCCEEEEEEECCEEEEECCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHE
LTDDGILINQHESPYYPSYAHEMKRAHSKIKGLFPITRVYQFHMPTYPSGHWLFGFASKK
EECCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCCEEEEECCCC
FDPLKDADLDAWNALGLKTKYYNTDIHTGAFALPTFVKEMLDDATE
CCCCCCCCCCCHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]