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The map label for this gene is hemL2

Identifier: 222151837

GI number: 222151837

Start: 1727030

End: 1728316

Strand: Direct

Name: hemL2

Synonym: MCCL_1594

Alternate gene names: 222151837

Gene position: 1727030-1728316 (Clockwise)

Preceding gene: 222151833

Following gene: 222151838

Centisome position: 82.15

GC content: 42.5

Gene sequence:

>1287_bases
ATGAACTTTAGTAATTCAGAAGCAATACAAAATCGTGCAAATGCCGCAATTCTTGGCGGTGTTAACTCACCTTCTCGTTC
TTATAAAGCAGTTGGTGGCGGTGCGCCTGTAGTGATGGCCCGTGGCGAAGGTGCTTACTTCTACGATGTGGATGGTAATA
AATATATTGATTATCTTGCAGCATACGGCCCGATTATAACAGGTCATGCACACCCACACATTACAGAAGCAATTAAACGT
GCTGCAGAAAACGGTGTACTATATGGTACTCCGACTGAACACGAAGTTGTGTTTGCAGAAATGTTGCAAGAAGCAATCCC
ATCATTAGAAAAAGTTCGCTTCGTTAACTCAGGTACGGAAGCTGTGATGACAACGATTCGTGTTGCGCGTGCATACACTG
GCAGAGATAAGATCATTAAATTTGCAGGATGCTATCACGGTCATTCAGATTTAGTGCTTGTGGCAGCAGGTAGTGGCCCT
TCACAGCTTGGTACTCCAGACTCAGCAGGTGTACCAAAGAGTGTGGCACAAGAAGTAATTACAGTCCCATTTAATGATAT
CGAACAATTCAAAGAAGCATTCTATCACTTCGGCGATCAGATTGCGGGTGTATTAGTTGAGCCAATCGTCGGTAACTTCG
GTATTGTAGAACCCCATGCAGGATTTCTAGAAGCAGTGAACGAAGTAGCACATAGTCACGGGGCGCTTGTTATCTATGAC
GAAGTTATCACTGCATTCCGTTTCTGCTACGGTGGTGCACAAGATATGCTTGGTGTAAAACCTGACTTGACAGCTTTCGG
TAAAGTTATTGGTGGCGGCTTACCGATTGGAGCATACGGTGGACGTCAGGATATCATGGAACATGTCGCTCCTCTTGGTC
CAACTTATCAGGCAGGTACAATGGCAGGTAATCCATTAAGTATGCAGGCAGGTATCGCTCTACTTGAAGTAATCAAAGCT
GAAGGCACTTATGATTATCTGGATAAGCTTGGTGAGATGCTTGAGACAGGCATGAATGAACTGATTGAAAAATACAGTAT
CAAAGCCAATATTAATCGTCTTAAAGGTGCTATGACAATTTATTTTACAGATGAAAAAGTAGAGAATTATGAACAAGCTG
AAGCGACGGATGGAGAAGCTTTTGCCAAATTCTTTAAATTGATGCTACATCAAGGAATCAACCTTGCACCTTCTAAATAC
GAGGCATGGTTCATTACAATCGCTCATACCGAACAAGATATTCAAGATACACTAATCGCAGTTGAAAACAGTTTCAAACA
AATGTAG

Upstream 100 bases:

>100_bases
GCATTCCTTTTTCTAACATAAATAACACTCCATCCTCAAATATTGTATAAGTATATGATAGTATAGTAGACGAAAATACA
AAACAAAAGAGGTCGATAAA

Downstream 100 bases:

>100_bases
CTTGAAAAAGTATGCAATGTCATGTATAAGTGTACTAACGAATAAATAAATGAGGTCAAAATATGAAATTAGGTGCACGT
ATATTCAAAACTGGAATTGC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA 2; Glutamate-1-semialdehyde aminotransferase 2; GSA-AT 2

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MNFSNSEAIQNRANAAILGGVNSPSRSYKAVGGGAPVVMARGEGAYFYDVDGNKYIDYLAAYGPIITGHAHPHITEAIKR
AAENGVLYGTPTEHEVVFAEMLQEAIPSLEKVRFVNSGTEAVMTTIRVARAYTGRDKIIKFAGCYHGHSDLVLVAAGSGP
SQLGTPDSAGVPKSVAQEVITVPFNDIEQFKEAFYHFGDQIAGVLVEPIVGNFGIVEPHAGFLEAVNEVAHSHGALVIYD
EVITAFRFCYGGAQDMLGVKPDLTAFGKVIGGGLPIGAYGGRQDIMEHVAPLGPTYQAGTMAGNPLSMQAGIALLEVIKA
EGTYDYLDKLGEMLETGMNELIEKYSIKANINRLKGAMTIYFTDEKVENYEQAEATDGEAFAKFFKLMLHQGINLAPSKY
EAWFITIAHTEQDIQDTLIAVENSFKQM

Sequences:

>Translated_428_residues
MNFSNSEAIQNRANAAILGGVNSPSRSYKAVGGGAPVVMARGEGAYFYDVDGNKYIDYLAAYGPIITGHAHPHITEAIKR
AAENGVLYGTPTEHEVVFAEMLQEAIPSLEKVRFVNSGTEAVMTTIRVARAYTGRDKIIKFAGCYHGHSDLVLVAAGSGP
SQLGTPDSAGVPKSVAQEVITVPFNDIEQFKEAFYHFGDQIAGVLVEPIVGNFGIVEPHAGFLEAVNEVAHSHGALVIYD
EVITAFRFCYGGAQDMLGVKPDLTAFGKVIGGGLPIGAYGGRQDIMEHVAPLGPTYQAGTMAGNPLSMQAGIALLEVIKA
EGTYDYLDKLGEMLETGMNELIEKYSIKANINRLKGAMTIYFTDEKVENYEQAEATDGEAFAKFFKLMLHQGINLAPSKY
EAWFITIAHTEQDIQDTLIAVENSFKQM
>Mature_428_residues
MNFSNSEAIQNRANAAILGGVNSPSRSYKAVGGGAPVVMARGEGAYFYDVDGNKYIDYLAAYGPIITGHAHPHITEAIKR
AAENGVLYGTPTEHEVVFAEMLQEAIPSLEKVRFVNSGTEAVMTTIRVARAYTGRDKIIKFAGCYHGHSDLVLVAAGSGP
SQLGTPDSAGVPKSVAQEVITVPFNDIEQFKEAFYHFGDQIAGVLVEPIVGNFGIVEPHAGFLEAVNEVAHSHGALVIYD
EVITAFRFCYGGAQDMLGVKPDLTAFGKVIGGGLPIGAYGGRQDIMEHVAPLGPTYQAGTMAGNPLSMQAGIALLEVIKA
EGTYDYLDKLGEMLETGMNELIEKYSIKANINRLKGAMTIYFTDEKVENYEQAEATDGEAFAKFFKLMLHQGINLAPSKY
EAWFITIAHTEQDIQDTLIAVENSFKQM

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=335, Percent_Identity=31.9402985074627, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI13994255, Length=361, Percent_Identity=27.9778393351801, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI37574042, Length=346, Percent_Identity=26.5895953757225, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI24119277, Length=337, Percent_Identity=26.1127596439169, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI284507298, Length=261, Percent_Identity=31.4176245210728, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI226442705, Length=342, Percent_Identity=25.7309941520468, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI226442709, Length=305, Percent_Identity=27.2131147540984, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1786349, Length=427, Percent_Identity=47.0725995316159, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI1789016, Length=393, Percent_Identity=28.498727735369, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1787560, Length=346, Percent_Identity=28.9017341040462, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI145693181, Length=333, Percent_Identity=30.03003003003, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788044, Length=331, Percent_Identity=29.607250755287, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1789759, Length=334, Percent_Identity=28.4431137724551, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1786991, Length=298, Percent_Identity=25.8389261744966, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25144271, Length=322, Percent_Identity=27.639751552795, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI32564660, Length=326, Percent_Identity=30.3680981595092, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI71992977, Length=432, Percent_Identity=24.7685185185185, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25144274, Length=167, Percent_Identity=28.7425149700599, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323470, Length=360, Percent_Identity=28.6111111111111, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6324432, Length=343, Percent_Identity=25.6559766763848, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21357415, Length=348, Percent_Identity=28.448275862069, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161085790, Length=364, Percent_Identity=27.1978021978022, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI28574759, Length=364, Percent_Identity=27.1978021978022, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI21356575, Length=344, Percent_Identity=27.0348837209302, Blast_Score=101, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA2_MACCJ (B9E7Y3)

Other databases:

- EMBL:   AP009484
- RefSeq:   YP_002560997.1
- ProteinModelPortal:   B9E7Y3
- GeneID:   7389745
- GenomeReviews:   AP009484_GR
- KEGG:   mcl:MCCL_1594
- OMA:   VENYEQA
- ProtClustDB:   PRK12389
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 46176; Mature: 46176

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFSNSEAIQNRANAAILGGVNSPSRSYKAVGGGAPVVMARGEGAYFYDVDGNKYIDYLA
CCCCCHHHHHHCCCEEEEECCCCCCCCCEECCCCCCEEEECCCCEEEEECCCCHHHHHHH
AYGPIITGHAHPHITEAIKRAAENGVLYGTPTEHEVVFAEMLQEAIPSLEKVRFVNSGTE
HHCCEEECCCCHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHCCHHHHHHHHCCCHH
AVMTTIRVARAYTGRDKIIKFAGCYHGHSDLVLVAAGSGPSQLGTPDSAGVPKSVAQEVI
HHHHHHHHHHHHCCHHHHEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHE
TVPFNDIEQFKEAFYHFGDQIAGVLVEPIVGNFGIVEPHAGFLEAVNEVAHSHGALVIYD
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEHH
EVITAFRFCYGGAQDMLGVKPDLTAFGKVIGGGLPIGAYGGRQDIMEHVAPLGPTYQAGT
HHHHHHHHHHCCHHHHCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
MAGNPLSMQAGIALLEVIKAEGTYDYLDKLGEMLETGMNELIEKYSIKANINRLKGAMTI
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
YFTDEKVENYEQAEATDGEAFAKFFKLMLHQGINLAPSKYEAWFITIAHTEQDIQDTLIA
EEECHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCHHHHHHHHHH
VENSFKQM
HHHHHHCC
>Mature Secondary Structure
MNFSNSEAIQNRANAAILGGVNSPSRSYKAVGGGAPVVMARGEGAYFYDVDGNKYIDYLA
CCCCCHHHHHHCCCEEEEECCCCCCCCCEECCCCCCEEEECCCCEEEEECCCCHHHHHHH
AYGPIITGHAHPHITEAIKRAAENGVLYGTPTEHEVVFAEMLQEAIPSLEKVRFVNSGTE
HHCCEEECCCCHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHCCHHHHHHHHCCCHH
AVMTTIRVARAYTGRDKIIKFAGCYHGHSDLVLVAAGSGPSQLGTPDSAGVPKSVAQEVI
HHHHHHHHHHHHCCHHHHEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHE
TVPFNDIEQFKEAFYHFGDQIAGVLVEPIVGNFGIVEPHAGFLEAVNEVAHSHGALVIYD
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEHH
EVITAFRFCYGGAQDMLGVKPDLTAFGKVIGGGLPIGAYGGRQDIMEHVAPLGPTYQAGT
HHHHHHHHHHCCHHHHCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
MAGNPLSMQAGIALLEVIKAEGTYDYLDKLGEMLETGMNELIEKYSIKANINRLKGAMTI
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
YFTDEKVENYEQAEATDGEAFAKFFKLMLHQGINLAPSKYEAWFITIAHTEQDIQDTLIA
EEECHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCHHHHHHHHHH
VENSFKQM
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA