Click here to switch to the map view.

The map label for this gene is pckA [H]

Identifier: 222151746

GI number: 222151746

Start: 1623528

End: 1625201

Strand: Direct

Name: pckA [H]

Synonym: MCCL_1499

Alternate gene names: 222151746

Gene position: 1623528-1625201 (Clockwise)

Preceding gene: 222151743

Following gene: 222151749

Centisome position: 77.23

GC content: 40.98

Gene sequence:

>1674_bases
ATGAAACCATCAAATCGATATGTTATACTATTCCATGAAACTGCTTACAAAAGCAGAACAATAACTTTTTGGAGGCTGAC
CATGGGGGAAAATACTCAAGTAATTGAGCAACTGAACAAATTAAACACTGGGAATTTAAAACGTCAATTATCAAGAACAC
AATTGATTACGGAAATTCTATTCAACGGTGAAGGTGTGTTAACAGAATCCGGTGCGGTACGTAGCGAAACGGGTAAATAT
ACTGGACGCTCACCGAAAGATAAGTTCATCGTGCGTGAAGCTGAAACTGAAGATAAAATCGACTGGGGCACAATCAACCA
GCCTATCGAAGAAGAAGTATTCTTAAACCTACTGGATAAAGTGACAGACTACTTAAACAAGAAAGAAAAAGTCTACGAAT
TTCAAGGGTATGCAGGTGCTGATAAATCATCACAGCTACAACTGCGCGTGGTGAACGAACTTGCTTGGCATAACTTGTTT
GCACGTACAATGTTTATCCGTCCTGAAACAACGGAAGAAGGATTAAACATTACACCTGACTTCACAATTATCTCTGCACC
ACACTTTAAGGCTGATCCATCAGTTGATGGCACGAAATCTGAAACATTCATCATCACAAGTTTCCGTCATAAGATCATAT
TAATTGGTGGTACAGAATATGCTGGCGAAATGAAAAAGGGTATCTTCGGTGTGATGAACTACTTATTACCGACACAGGGT
ATTATGAGTATGCACTGTTCAGCAAACGTTGGAGAGAATAAGGACGTTGCGTTATTCTTCGGGTTATCTGGAACAGGTAA
AACGACGTTATCTGCAGATCCACAACGTAAATTAATCGGTGATGATGAGCACGGTTGGAACGACAACGGTGTATTCAATA
TCGAAGGCGGCTGCTATGCGAAGACTATTGACTTATCACGCGAGAAGGAACCCCAGATCTACGATGCAATTCGTTACGGT
GCCATCCTCGAGAATGTTAAGGTCGATGAAGAAGGTGTGCCGGACTATACGGATAAATCTTTAACTGAGAATACTCGTGC
AGCTTATCCAATCGATAATATCGATAATATCGTTCTACCATCAATTGCAGGACATGCGAAGACAATCATCTTCTTAACTG
CTGATGCGTTCGGTGTGTTACCTCCAATCTCTAAATTAACGAAAGAACAAGCGATGTATCACTTCTTAAGTGGATTTACA
TCTAAATTAGCAGGAACAGAACGCGGTGTAACAAGCCCGCAACCAGTATTCTCTACATGTTTCGGTTCACCGTTCTTACC
ATTACATGCAACAGTGTATGCAAACATGTTAGGGGATCTAATCGATAAACATGGTGCAGCAGTTTATCTTGTGAATACAG
GATGGACTGGCGGAGAGTATGGTGTTGGTAGCCGTATGAACTTAACACATACACGTTCTATGATTCGACGTGCCATTAGC
GGCGAACTGAAGACAGTTGAATATCATCAGGATGAAATCTTTGGACTTAATATTCCGAAGCATGTACCAGGTGTACCGGC
TGAGATTCTGTATCCTCGTCATGCATGGGTATCCCAAGATGCATATGATGAGAAAGCAAAAGATTTAGCATTACGCTTCC
AGGAGAACTTTAAGAAGTTTGGTGAGAAGTCAGCTGAAATCGAAGAAAAAGGCGGATTTAATTTTAGAGGTTAG

Upstream 100 bases:

>100_bases
CAAGTTAGATAATACTAAACTTCCTGTTCACTGTCAACAATACGACTTATAGATATAATTTTCTGAATTATTTATTATAT
AGATTAAATACATTTATTAT

Downstream 100 bases:

>100_bases
CATAAAAAAACGTCACTTTTTTAAAGTGGCGTTTTTTTAATCTATTAATTTATGAGTTTATTTCCTTCTCCACTTCTTCC
ATCCACCTTTCAATTTCCTG

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MKPSNRYVILFHETAYKSRTITFWRLTMGENTQVIEQLNKLNTGNLKRQLSRTQLITEILFNGEGVLTESGAVRSETGKY
TGRSPKDKFIVREAETEDKIDWGTINQPIEEEVFLNLLDKVTDYLNKKEKVYEFQGYAGADKSSQLQLRVVNELAWHNLF
ARTMFIRPETTEEGLNITPDFTIISAPHFKADPSVDGTKSETFIITSFRHKIILIGGTEYAGEMKKGIFGVMNYLLPTQG
IMSMHCSANVGENKDVALFFGLSGTGKTTLSADPQRKLIGDDEHGWNDNGVFNIEGGCYAKTIDLSREKEPQIYDAIRYG
AILENVKVDEEGVPDYTDKSLTENTRAAYPIDNIDNIVLPSIAGHAKTIIFLTADAFGVLPPISKLTKEQAMYHFLSGFT
SKLAGTERGVTSPQPVFSTCFGSPFLPLHATVYANMLGDLIDKHGAAVYLVNTGWTGGEYGVGSRMNLTHTRSMIRRAIS
GELKTVEYHQDEIFGLNIPKHVPGVPAEILYPRHAWVSQDAYDEKAKDLALRFQENFKKFGEKSAEIEEKGGFNFRG

Sequences:

>Translated_557_residues
MKPSNRYVILFHETAYKSRTITFWRLTMGENTQVIEQLNKLNTGNLKRQLSRTQLITEILFNGEGVLTESGAVRSETGKY
TGRSPKDKFIVREAETEDKIDWGTINQPIEEEVFLNLLDKVTDYLNKKEKVYEFQGYAGADKSSQLQLRVVNELAWHNLF
ARTMFIRPETTEEGLNITPDFTIISAPHFKADPSVDGTKSETFIITSFRHKIILIGGTEYAGEMKKGIFGVMNYLLPTQG
IMSMHCSANVGENKDVALFFGLSGTGKTTLSADPQRKLIGDDEHGWNDNGVFNIEGGCYAKTIDLSREKEPQIYDAIRYG
AILENVKVDEEGVPDYTDKSLTENTRAAYPIDNIDNIVLPSIAGHAKTIIFLTADAFGVLPPISKLTKEQAMYHFLSGFT
SKLAGTERGVTSPQPVFSTCFGSPFLPLHATVYANMLGDLIDKHGAAVYLVNTGWTGGEYGVGSRMNLTHTRSMIRRAIS
GELKTVEYHQDEIFGLNIPKHVPGVPAEILYPRHAWVSQDAYDEKAKDLALRFQENFKKFGEKSAEIEEKGGFNFRG
>Mature_557_residues
MKPSNRYVILFHETAYKSRTITFWRLTMGENTQVIEQLNKLNTGNLKRQLSRTQLITEILFNGEGVLTESGAVRSETGKY
TGRSPKDKFIVREAETEDKIDWGTINQPIEEEVFLNLLDKVTDYLNKKEKVYEFQGYAGADKSSQLQLRVVNELAWHNLF
ARTMFIRPETTEEGLNITPDFTIISAPHFKADPSVDGTKSETFIITSFRHKIILIGGTEYAGEMKKGIFGVMNYLLPTQG
IMSMHCSANVGENKDVALFFGLSGTGKTTLSADPQRKLIGDDEHGWNDNGVFNIEGGCYAKTIDLSREKEPQIYDAIRYG
AILENVKVDEEGVPDYTDKSLTENTRAAYPIDNIDNIVLPSIAGHAKTIIFLTADAFGVLPPISKLTKEQAMYHFLSGFT
SKLAGTERGVTSPQPVFSTCFGSPFLPLHATVYANMLGDLIDKHGAAVYLVNTGWTGGEYGVGSRMNLTHTRSMIRRAIS
GELKTVEYHQDEIFGLNIPKHVPGVPAEILYPRHAWVSQDAYDEKAKDLALRFQENFKKFGEKSAEIEEKGGFNFRG

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=493, Percent_Identity=52.1298174442191, Blast_Score=496, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6322950, Length=505, Percent_Identity=48.9108910891089, Blast_Score=474, Evalue=1e-134,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 62278; Mature: 62278

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSNRYVILFHETAYKSRTITFWRLTMGENTQVIEQLNKLNTGNLKRQLSRTQLITEIL
CCCCCCEEEEEECCCCCCCEEEEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
FNGEGVLTESGAVRSETGKYTGRSPKDKFIVREAETEDKIDWGTINQPIEEEVFLNLLDK
HCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
VTDYLNKKEKVYEFQGYAGADKSSQLQLRVVNELAWHNLFARTMFIRPETTEEGLNITPD
HHHHHCCHHHEEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCC
FTIISAPHFKADPSVDGTKSETFIITSFRHKIILIGGTEYAGEMKKGIFGVMNYLLPTQG
EEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEEEECCHHHHHHHHHHHHHHHHHHCCCC
IMSMHCSANVGENKDVALFFGLSGTGKTTLSADPQRKLIGDDEHGWNDNGVFNIEGGCYA
HHEEECCCCCCCCCCEEEEEEECCCCCCEECCCCCHHCCCCCCCCCCCCCEEEECCCCEE
KTIDLSREKEPQIYDAIRYGAILENVKVDEEGVPDYTDKSLTENTRAAYPIDNIDNIVLP
EEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECC
SIAGHAKTIIFLTADAFGVLPPISKLTKEQAMYHFLSGFTSKLAGTERGVTSPQPVFSTC
CCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
FGSPFLPLHATVYANMLGDLIDKHGAAVYLVNTGWTGGEYGVGSRMNLTHTRSMIRRAIS
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHC
GELKTVEYHQDEIFGLNIPKHVPGVPAEILYPRHAWVSQDAYDEKAKDLALRFQENFKKF
CCCEEEEECCCCEEECCCCCCCCCCCHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHH
GEKSAEIEEKGGFNFRG
CCCCCCHHHCCCCCCCC
>Mature Secondary Structure
MKPSNRYVILFHETAYKSRTITFWRLTMGENTQVIEQLNKLNTGNLKRQLSRTQLITEIL
CCCCCCEEEEEECCCCCCCEEEEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
FNGEGVLTESGAVRSETGKYTGRSPKDKFIVREAETEDKIDWGTINQPIEEEVFLNLLDK
HCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
VTDYLNKKEKVYEFQGYAGADKSSQLQLRVVNELAWHNLFARTMFIRPETTEEGLNITPD
HHHHHCCHHHEEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCC
FTIISAPHFKADPSVDGTKSETFIITSFRHKIILIGGTEYAGEMKKGIFGVMNYLLPTQG
EEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEEEECCHHHHHHHHHHHHHHHHHHCCCC
IMSMHCSANVGENKDVALFFGLSGTGKTTLSADPQRKLIGDDEHGWNDNGVFNIEGGCYA
HHEEECCCCCCCCCCEEEEEEECCCCCCEECCCCCHHCCCCCCCCCCCCCEEEECCCCEE
KTIDLSREKEPQIYDAIRYGAILENVKVDEEGVPDYTDKSLTENTRAAYPIDNIDNIVLP
EEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECC
SIAGHAKTIIFLTADAFGVLPPISKLTKEQAMYHFLSGFTSKLAGTERGVTSPQPVFSTC
CCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
FGSPFLPLHATVYANMLGDLIDKHGAAVYLVNTGWTGGEYGVGSRMNLTHTRSMIRRAIS
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHC
GELKTVEYHQDEIFGLNIPKHVPGVPAEILYPRHAWVSQDAYDEKAKDLALRFQENFKKF
CCCEEEEECCCCEEECCCCCCCCCCCHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHH
GEKSAEIEEKGGFNFRG
CCCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA