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The map label for this gene is grlB [H]

Identifier: 222151258

GI number: 222151258

Start: 1114205

End: 1116220

Strand: Direct

Name: grlB [H]

Synonym: MCCL_1011

Alternate gene names: 222151258

Gene position: 1114205-1116220 (Clockwise)

Preceding gene: 222151256

Following gene: 222151259

Centisome position: 53.0

GC content: 39.63

Gene sequence:

>2016_bases
TTGGCAAATAATCAGTATAATGATGAATCCATTCAAGTCTTAGAAGGTCTTGAGGCAGTTAGAAAAAGACCAGGGATGTA
TATAGGATCTACAGATTCAAGAGGACTACATCACCTTGTATATGAAATTCTTGATAATTCAATTGACGAAGCATTGAATG
GTCACGGTAATGAAATCAATGTTGTTCTCAATGCAGATGACTCTGTGACAGTAACAGATAATGGGCGTGGTATGCCGACA
GGACTGCATAAGACCGGTAAACCAACACCAGAAGTAATATTTACTGTGCTGCATGCAGGAGGGAAATTTGGTCAAGGAGG
CTATAAAACAACAGGAGGACTGCATGGTGTAGGTGCTTCTGTTGTTAATGCGTTGAGCAGCTGGCTCGAGGTGACGATAC
ATAATAATGGAAAGATATTCAAGCAGCGCTTTCAAGATGGTGGCGTACCCGTTACAACGTTAGAAGAAATCGGAAAATCA
AAAAAGACGGGGACGACTGTGACATTTAAACCTGACCCATCTATTTTTAAACAGACAGTTAAATTTAATTTTGATACGTT
AAGTGAAAGATTACGCGAATCTGCATTTCTACTTAATAATCTCAAAATCACGATCAGTGATCTTAGAAATAATGAGGAAC
GTCATGAAGTATTCTATTATGAGGATGGATTAAAAGATTTTGTCAACTTTGTCAACGAAGGCAAAGAAACGTTACATGAA
GTTGCGAAGTTTAGCGGTAAGTCACATGATATTGAAGTTGAAGTTGCATTCCAGTTTAACGATCAATACTCAGAGACTAT
TCTGTCATTTGTTAATAACGTGAGAACGAAGGATGGCGGGACACATGAGGTTGGAATGAAGACCGCTATCACACGAGTAT
TTAATGAATATGCCAGAAAGATCGGAGAGTTGAAGGCTAAGGATAAGAATCTGGATGGTAATGATATTCGTGAAGGGATG
ACCGCGATCATTTCGGTTAAGATTCCCGAAAATTTACTGCAGTTTGAAGGGCAGACGAAATCGAAGCTTGGAACGAGTGA
AGCACGTAGTGCAGTGGATGGTGTATTGAGCGAACAACTTCCGTTCTATTTTGAGGAAAACGGAGTACTAAGTCAGAAAT
TAGTGAAGAAAGCGGTAAAAGCAGCTCAGGCAAGAGATGCTGCACGTAAAGCGCGTGAAGAAGCGCGAAATGGTAAGAAG
AATAAACGTAAAGAAACATTATTGTCAGGTAAACTCACACCTGCACAGAGTAAGGATACGAGTCGTAACGAGCTTTACCT
TGTCGAAGGAGATTCAGCAGGTGGTTCTGCTAAACTCGGGCGTGATCGTAAGTTTCAGGCGATACTTCCGTTACGTGGTA
AAGTTATCAATACAGAAAAAGCAAAGCTCGAAGATATTCTGAAGAATGAAGAAATCAGTACCATTATTCATACGATTGGT
GCAGGGGTCGGAAATGATTTTGAAGTTAAAGATGCAAACTATGACAAAATCATTATAATGACCGATGCTGACACAGATGG
TGCACATATTCAGGTGCTGCTTCTGACATTCTTCTTTAAATATATGAAACCTTTGATTGATGCCGGCAAAGTTTATATTG
CATTACCACCGTTGTATAAAGTTTCTAAAGGTAAAGGGAAGAACGAAGTTATTGAATATGCCTGGACAGACGATGAACTT
AAAGGAGTTCAAAAGAAGGTAGGTAAGGGCTTTATTCTACAGCGATATAAAGGACTTGGAGAAATGAATGCAGATCAACT
GTGGGATACTACGATGAATCCTGAAACGAGAACATTAATTCGTGTTGAAATAGATGACGAACTTAGAAGTGCTAAACGTG
TTTCGACATTAATGGGAGATAAGGTTGAACCGCGACGTGACTGGATTGAACGCCATGTTGAGTTCGGTATGCAGGAAGAT
GTAAGCATTCTGGAAAACGATGAAGTCGAACGTTTAACAGAAATAGAGGATATCGAAACAATTCAGGATGATGAAAAGGG
TGAGGATAATGAATGA

Upstream 100 bases:

>100_bases
AAAATAATTACGTTTTGTAAAGACTTGCATTTTTAAGGGTTTCATTTAAAATAATGAATGTATATAAATTTTCGAACGTA
TGTTTGTAGGAGGTAAGCAC

Downstream 100 bases:

>100_bases
GAATATACAAAGTCTTCCACTTGAAGATGTAATCGGTGATCGTTTTGGTCGCTATAGTAAATATATTATACAGGATCGAG
CGATTCCGGATGTACGTGAT

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 671; Mature: 670

Protein sequence:

>671_residues
MANNQYNDESIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVYEILDNSIDEALNGHGNEINVVLNADDSVTVTDNGRGMPT
GLHKTGKPTPEVIFTVLHAGGKFGQGGYKTTGGLHGVGASVVNALSSWLEVTIHNNGKIFKQRFQDGGVPVTTLEEIGKS
KKTGTTVTFKPDPSIFKQTVKFNFDTLSERLRESAFLLNNLKITISDLRNNEERHEVFYYEDGLKDFVNFVNEGKETLHE
VAKFSGKSHDIEVEVAFQFNDQYSETILSFVNNVRTKDGGTHEVGMKTAITRVFNEYARKIGELKAKDKNLDGNDIREGM
TAIISVKIPENLLQFEGQTKSKLGTSEARSAVDGVLSEQLPFYFEENGVLSQKLVKKAVKAAQARDAARKAREEARNGKK
NKRKETLLSGKLTPAQSKDTSRNELYLVEGDSAGGSAKLGRDRKFQAILPLRGKVINTEKAKLEDILKNEEISTIIHTIG
AGVGNDFEVKDANYDKIIIMTDADTDGAHIQVLLLTFFFKYMKPLIDAGKVYIALPPLYKVSKGKGKNEVIEYAWTDDEL
KGVQKKVGKGFILQRYKGLGEMNADQLWDTTMNPETRTLIRVEIDDELRSAKRVSTLMGDKVEPRRDWIERHVEFGMQED
VSILENDEVERLTEIEDIETIQDDEKGEDNE

Sequences:

>Translated_671_residues
MANNQYNDESIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVYEILDNSIDEALNGHGNEINVVLNADDSVTVTDNGRGMPT
GLHKTGKPTPEVIFTVLHAGGKFGQGGYKTTGGLHGVGASVVNALSSWLEVTIHNNGKIFKQRFQDGGVPVTTLEEIGKS
KKTGTTVTFKPDPSIFKQTVKFNFDTLSERLRESAFLLNNLKITISDLRNNEERHEVFYYEDGLKDFVNFVNEGKETLHE
VAKFSGKSHDIEVEVAFQFNDQYSETILSFVNNVRTKDGGTHEVGMKTAITRVFNEYARKIGELKAKDKNLDGNDIREGM
TAIISVKIPENLLQFEGQTKSKLGTSEARSAVDGVLSEQLPFYFEENGVLSQKLVKKAVKAAQARDAARKAREEARNGKK
NKRKETLLSGKLTPAQSKDTSRNELYLVEGDSAGGSAKLGRDRKFQAILPLRGKVINTEKAKLEDILKNEEISTIIHTIG
AGVGNDFEVKDANYDKIIIMTDADTDGAHIQVLLLTFFFKYMKPLIDAGKVYIALPPLYKVSKGKGKNEVIEYAWTDDEL
KGVQKKVGKGFILQRYKGLGEMNADQLWDTTMNPETRTLIRVEIDDELRSAKRVSTLMGDKVEPRRDWIERHVEFGMQED
VSILENDEVERLTEIEDIETIQDDEKGEDNE
>Mature_670_residues
ANNQYNDESIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVYEILDNSIDEALNGHGNEINVVLNADDSVTVTDNGRGMPTG
LHKTGKPTPEVIFTVLHAGGKFGQGGYKTTGGLHGVGASVVNALSSWLEVTIHNNGKIFKQRFQDGGVPVTTLEEIGKSK
KTGTTVTFKPDPSIFKQTVKFNFDTLSERLRESAFLLNNLKITISDLRNNEERHEVFYYEDGLKDFVNFVNEGKETLHEV
AKFSGKSHDIEVEVAFQFNDQYSETILSFVNNVRTKDGGTHEVGMKTAITRVFNEYARKIGELKAKDKNLDGNDIREGMT
AIISVKIPENLLQFEGQTKSKLGTSEARSAVDGVLSEQLPFYFEENGVLSQKLVKKAVKAAQARDAARKAREEARNGKKN
KRKETLLSGKLTPAQSKDTSRNELYLVEGDSAGGSAKLGRDRKFQAILPLRGKVINTEKAKLEDILKNEEISTIIHTIGA
GVGNDFEVKDANYDKIIIMTDADTDGAHIQVLLLTFFFKYMKPLIDAGKVYIALPPLYKVSKGKGKNEVIEYAWTDDELK
GVQKKVGKGFILQRYKGLGEMNADQLWDTTMNPETRTLIRVEIDDELRSAKRVSTLMGDKVEPRRDWIERHVEFGMQEDV
SILENDEVERLTEIEDIETIQDDEKGEDNE

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=623, Percent_Identity=26.1637239165329, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI19913408, Length=685, Percent_Identity=24.963503649635, Blast_Score=125, Evalue=1e-28,
Organism=Escherichia coli, GI48994957, Length=551, Percent_Identity=48.6388384754991, Blast_Score=493, Evalue=1e-140,
Organism=Escherichia coli, GI1789408, Length=639, Percent_Identity=38.4976525821596, Blast_Score=410, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17535065, Length=664, Percent_Identity=25.3012048192771, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI212645845, Length=614, Percent_Identity=25.8957654723127, Blast_Score=112, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI212645657, Length=204, Percent_Identity=28.921568627451, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=627, Percent_Identity=28.0701754385965, Blast_Score=121, Evalue=4e-28,
Organism=Drosophila melanogaster, GI17136538, Length=678, Percent_Identity=24.0412979351032, Blast_Score=119, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005740 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.-

Molecular weight: Translated: 74959; Mature: 74828

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANNQYNDESIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVYEILDNSIDEALNGHGNEIN
CCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEE
VVLNADDSVTVTDNGRGMPTGLHKTGKPTPEVIFTVLHAGGKFGQGGYKTTGGLHGVGAS
EEEECCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
VVNALSSWLEVTIHNNGKIFKQRFQDGGVPVTTLEEIGKSKKTGTTVTFKPDPSIFKQTV
HHHHHHHHEEEEECCCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCEEEECCCHHHHHHHH
KFNFDTLSERLRESAFLLNNLKITISDLRNNEERHEVFYYEDGLKDFVNFVNEGKETLHE
HCCHHHHHHHHHHHHHHHHCCEEEEEHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHH
VAKFSGKSHDIEVEVAFQFNDQYSETILSFVNNVRTKDGGTHEVGMKTAITRVFNEYARK
HHHHCCCCCCEEEEEEEEECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
IGELKAKDKNLDGNDIREGMTAIISVKIPENLLQFEGQTKSKLGTSEARSAVDGVLSEQL
HHHHHCCCCCCCHHHHHHHHEEEEEEECCHHHHHCCCCHHHHCCHHHHHHHHHHHHHHCC
PFYFEENGVLSQKLVKKAVKAAQARDAARKAREEARNGKKNKRKETLLSGKLTPAQSKDT
CEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCC
SRNELYLVEGDSAGGSAKLGRDRKFQAILPLRGKVINTEKAKLEDILKNEEISTIIHTIG
CCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEECCHHHHHHHHHCCCHHHHHHHHHH
AGVGNDFEVKDANYDKIIIMTDADTDGAHIQVLLLTFFFKYMKPLIDAGKVYIALPPLYK
CCCCCCEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEE
VSKGKGKNEVIEYAWTDDELKGVQKKVGKGFILQRYKGLGEMNADQLWDTTMNPETRTLI
CCCCCCCCCEEEEECCCHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHCCCCCCCCEEEE
RVEIDDELRSAKRVSTLMGDKVEPRRDWIERHVEFGMQEDVSILENDEVERLTEIEDIET
EEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHH
IQDDEKGEDNE
HCCCCCCCCCC
>Mature Secondary Structure 
ANNQYNDESIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVYEILDNSIDEALNGHGNEIN
CCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCEEE
VVLNADDSVTVTDNGRGMPTGLHKTGKPTPEVIFTVLHAGGKFGQGGYKTTGGLHGVGAS
EEEECCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
VVNALSSWLEVTIHNNGKIFKQRFQDGGVPVTTLEEIGKSKKTGTTVTFKPDPSIFKQTV
HHHHHHHHEEEEECCCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCEEEECCCHHHHHHHH
KFNFDTLSERLRESAFLLNNLKITISDLRNNEERHEVFYYEDGLKDFVNFVNEGKETLHE
HCCHHHHHHHHHHHHHHHHCCEEEEEHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHH
VAKFSGKSHDIEVEVAFQFNDQYSETILSFVNNVRTKDGGTHEVGMKTAITRVFNEYARK
HHHHCCCCCCEEEEEEEEECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
IGELKAKDKNLDGNDIREGMTAIISVKIPENLLQFEGQTKSKLGTSEARSAVDGVLSEQL
HHHHHCCCCCCCHHHHHHHHEEEEEEECCHHHHHCCCCHHHHCCHHHHHHHHHHHHHHCC
PFYFEENGVLSQKLVKKAVKAAQARDAARKAREEARNGKKNKRKETLLSGKLTPAQSKDT
CEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCC
SRNELYLVEGDSAGGSAKLGRDRKFQAILPLRGKVINTEKAKLEDILKNEEISTIIHTIG
CCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEECCHHHHHHHHHCCCHHHHHHHHHH
AGVGNDFEVKDANYDKIIIMTDADTDGAHIQVLLLTFFFKYMKPLIDAGKVYIALPPLYK
CCCCCCEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEE
VSKGKGKNEVIEYAWTDDELKGVQKKVGKGFILQRYKGLGEMNADQLWDTTMNPETRTLI
CCCCCCCCCEEEEECCCHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHCCCCCCCCEEEE
RVEIDDELRSAKRVSTLMGDKVEPRRDWIERHVEFGMQEDVSILENDEVERLTEIEDIET
EEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHH
IQDDEKGEDNE
HCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA