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The map label for this gene is nusA [H]

Identifier: 222151100

GI number: 222151100

Start: 931110

End: 932171

Strand: Direct

Name: nusA [H]

Synonym: MCCL_0851

Alternate gene names: 222151100

Gene position: 931110-932171 (Clockwise)

Preceding gene: 222151099

Following gene: 222151101

Centisome position: 44.29

GC content: 39.45

Gene sequence:

>1062_bases
ATGAAAAACAATGAATTATTAAATGCGATTGATTTTCTTGAAAAAGAAAAATCGATTCCAAGAGAAGTACTGATAGAAAC
GATTGAGGCAGCTTTGATTACTGCTTATAAGAAAAACTATAATTCGGCAAACAATGTACGTGTTGAACTGAATATGGATA
ACGGAGCATACCGAGTATATTCACGTAAGAACGTAGTTGAAGAAGTAATGAACTCAAGAGAAGAAGTAAGTCTTGAAACA
GCATTATTATCGAACCCTGCATATGAAATCGGCGATATATATGAAGAGGATGTTACACCTAAAGATTTTGGACGCGTTTC
AGCGCAAGCTGCAAAGCAAGCAGTATTACAAAGATTAAGAGATGCAGAACGTGGTATATTATATAATGAGTTTATCGATA
AGGAAGATGATATTATGACTGGTATCATCGACCGAGTGGATCATCGTTATGTCTATGTAACTTTAGGTAAGACAGAGGCT
GTTTTATCTGAAGCAGAAAGAAGTCCGAATGAAGTTTATCGTCCGACAGAACGAATTAAGGTCTATGTCAATAAAGTGGA
ACAGACGACTAAAGGACCTCAAATCTTTGTGTCACGTAGTCATCCAGGATTATTAAAGCGTTTATTCGAACAAGAAGTTC
CTGAAATTTTTGATGGTACGGTAGTGGTGAAATCTGTAGCTCGTGAAGCAGGAGATCGCTCGAAGATCAGTGTCTATTCG
GATAATCCTGATATCGATGCTGTGGGTGCATGTGTGGGTGCGAAAGGTGCACGTGTTGAAGCAGTTGTGGAAGAACTCGG
TGGAGAAAAAATTGACATCGTTGAATGGAGTGCAGATACGAAGACTTTCGTTAAGAATGCATTGAGTCCATCTCAAGTTG
TCGATGTATTGGTAGACGAAGCGAACCAGTCAACAACAGTAATCGTTCCAGATTACCAGTTGTCCCTCGCTATTGGTAAG
AAAGGTCAGAATGCACGTCTTGCTGCTAAGCTAACGGGCTGGAAGATTGATATTAAATCTGAGACAGATGCTAAAGAAGC
GGGCATTTATCCGGCAGAATAG

Upstream 100 bases:

>100_bases
AACATTATCAGCAAAGATTAAAACGAGAACAAAAGTGATTGAAATTGACCGTAAGCGTATTGCTAAGATTCGTCTTGCAG
TCATTCTATAGGAGGCAGTT

Downstream 100 bases:

>100_bases
GGGGATTTGATGAAACAACGTAAAATACCCATGAGAAAATGTATTCTTTCGAATGAAATGAAACCTAAGAAAGAAATGAT
TCGTGTCGTTAAAAATAAAG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MKNNELLNAIDFLEKEKSIPREVLIETIEAALITAYKKNYNSANNVRVELNMDNGAYRVYSRKNVVEEVMNSREEVSLET
ALLSNPAYEIGDIYEEDVTPKDFGRVSAQAAKQAVLQRLRDAERGILYNEFIDKEDDIMTGIIDRVDHRYVYVTLGKTEA
VLSEAERSPNEVYRPTERIKVYVNKVEQTTKGPQIFVSRSHPGLLKRLFEQEVPEIFDGTVVVKSVAREAGDRSKISVYS
DNPDIDAVGACVGAKGARVEAVVEELGGEKIDIVEWSADTKTFVKNALSPSQVVDVLVDEANQSTTVIVPDYQLSLAIGK
KGQNARLAAKLTGWKIDIKSETDAKEAGIYPAE

Sequences:

>Translated_353_residues
MKNNELLNAIDFLEKEKSIPREVLIETIEAALITAYKKNYNSANNVRVELNMDNGAYRVYSRKNVVEEVMNSREEVSLET
ALLSNPAYEIGDIYEEDVTPKDFGRVSAQAAKQAVLQRLRDAERGILYNEFIDKEDDIMTGIIDRVDHRYVYVTLGKTEA
VLSEAERSPNEVYRPTERIKVYVNKVEQTTKGPQIFVSRSHPGLLKRLFEQEVPEIFDGTVVVKSVAREAGDRSKISVYS
DNPDIDAVGACVGAKGARVEAVVEELGGEKIDIVEWSADTKTFVKNALSPSQVVDVLVDEANQSTTVIVPDYQLSLAIGK
KGQNARLAAKLTGWKIDIKSETDAKEAGIYPAE
>Mature_353_residues
MKNNELLNAIDFLEKEKSIPREVLIETIEAALITAYKKNYNSANNVRVELNMDNGAYRVYSRKNVVEEVMNSREEVSLET
ALLSNPAYEIGDIYEEDVTPKDFGRVSAQAAKQAVLQRLRDAERGILYNEFIDKEDDIMTGIIDRVDHRYVYVTLGKTEA
VLSEAERSPNEVYRPTERIKVYVNKVEQTTKGPQIFVSRSHPGLLKRLFEQEVPEIFDGTVVVKSVAREAGDRSKISVYS
DNPDIDAVGACVGAKGARVEAVVEELGGEKIDIVEWSADTKTFVKNALSPSQVVDVLVDEANQSTTVIVPDYQLSLAIGK
KGQNARLAAKLTGWKIDIKSETDAKEAGIYPAE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=342, Percent_Identity=38.5964912280702, Blast_Score=263, Evalue=1e-71,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 39307; Mature: 39307

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50084 KH_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNNELLNAIDFLEKEKSIPREVLIETIEAALITAYKKNYNSANNVRVELNMDNGAYRVY
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEE
SRKNVVEEVMNSREEVSLETALLSNPAYEIGDIYEEDVTPKDFGRVSAQAAKQAVLQRLR
HHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
DAERGILYNEFIDKEDDIMTGIIDRVDHRYVYVTLGKTEAVLSEAERSPNEVYRPTERIK
HHHCCCCHHHHCCCCHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHCCCHHHHCCHHHHH
VYVNKVEQTTKGPQIFVSRSHPGLLKRLFEQEVPEIFDGTVVVKSVAREAGDRSKISVYS
HHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEC
DNPDIDAVGACVGAKGARVEAVVEELGGEKIDIVEWSADTKTFVKNALSPSQVVDVLVDE
CCCCHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCHHHHHHHCCCHHHHHHHHHHC
ANQSTTVIVPDYQLSLAIGKKGQNARLAAKLTGWKIDIKSETDAKEAGIYPAE
CCCCCEEEECCCEEEEEECCCCCCCEEEEEEECEEEEECCCCCHHHCCCCCCC
>Mature Secondary Structure
MKNNELLNAIDFLEKEKSIPREVLIETIEAALITAYKKNYNSANNVRVELNMDNGAYRVY
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEE
SRKNVVEEVMNSREEVSLETALLSNPAYEIGDIYEEDVTPKDFGRVSAQAAKQAVLQRLR
HHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
DAERGILYNEFIDKEDDIMTGIIDRVDHRYVYVTLGKTEAVLSEAERSPNEVYRPTERIK
HHHCCCCHHHHCCCCHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHCCCHHHHCCHHHHH
VYVNKVEQTTKGPQIFVSRSHPGLLKRLFEQEVPEIFDGTVVVKSVAREAGDRSKISVYS
HHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEC
DNPDIDAVGACVGAKGARVEAVVEELGGEKIDIVEWSADTKTFVKNALSPSQVVDVLVDE
CCCCHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCHHHHHHHCCCHHHHHHHHHHC
ANQSTTVIVPDYQLSLAIGKKGQNARLAAKLTGWKIDIKSETDAKEAGIYPAE
CCCCCEEEECCCEEEEEECCCCCCCEEEEEEECEEEEECCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8491709; 9384377 [H]